Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005025A_C01 KMC005025A_c01
(664 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q41738|TH41_MAIZE Thiazole biosynthetic enzyme 1-1, chloropla... 165 6e-40
sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplast p... 164 8e-40
dbj|BAC45141.1| putative thiamine biosynthetic enzyme [Oryza sat... 163 2e-39
sp|Q41739|TH42_MAIZE Thiazole biosynthetic enzyme 1-2, chloropla... 163 2e-39
sp|Q38709|THI4_ALNGL Thiazole biosynthetic enzyme, chloroplast p... 161 8e-39
>sp|Q41738|TH41_MAIZE Thiazole biosynthetic enzyme 1-1, chloroplast precursor
gi|2130146|pir||S61419 thiamin biosynthesis protein
thi1-1 - maize gi|596078|gb|AAA96738.1| thiamine
biosynthetic enzyme
Length = 354
Score = 165 bits (417), Expect = 6e-40
Identities = 94/149 (63%), Positives = 104/149 (69%), Gaps = 9/149 (6%)
Frame = -1
Query: 664 VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPG 485
V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDGPFGATGVKRL+ IGMI +VPG
Sbjct: 203 VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLQDIGMISAVPG 262
Query: 484 MKALDMNTADRRYC*VHQ--------GGCTRNDSDWHGGS*N*WCPQHSGPTFGAMMISG 329
MKALDMNTA+ + + G + D GPTFGAMMISG
Sbjct: 263 MKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDG---------APRMGPTFGAMMISG 313
Query: 328 QKAAHLALKALGKNTCLIDGTCE-AVREE 245
QKAAHLALKALG+ +DGT +REE
Sbjct: 314 QKAAHLALKALGRPNA-VDGTMSPPLREE 341
Score = 53.9 bits (128), Expect = 2e-06
Identities = 26/28 (92%), Positives = 26/28 (92%)
Frame = -2
Query: 453 DAIVRFTREVVPGMIVTGMEVAEIDGAP 370
D IVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 273 DEIVRLTREVVPGMIVTGMEVAEIDGAP 300
>sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplast precursor
gi|7446894|pir||T10474 thiamin biosynthesis protein thi1
- sweet orange gi|2582665|emb|CAB05370.1| thi [Citrus
sinensis]
Length = 356
Score = 164 bits (416), Expect = 8e-40
Identities = 92/141 (65%), Positives = 101/141 (71%), Gaps = 8/141 (5%)
Frame = -1
Query: 664 VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPG 485
V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDGPFGATGVKRLKSIGMI+ VPG
Sbjct: 204 VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPG 263
Query: 484 MKALDMNTADRRYC*VHQ--------GGCTRNDSDWHGGS*N*WCPQHSGPTFGAMMISG 329
MKALDMN+A+ + + G + D GPTFGAMMISG
Sbjct: 264 MKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDG---------APRMGPTFGAMMISG 314
Query: 328 QKAAHLALKALGKNTCLIDGT 266
QKAAHLALK+LG+ L DGT
Sbjct: 315 QKAAHLALKSLGQPNAL-DGT 334
Score = 55.8 bits (133), Expect = 5e-07
Identities = 27/28 (96%), Positives = 27/28 (96%)
Frame = -2
Query: 453 DAIVRFTREVVPGMIVTGMEVAEIDGAP 370
DAIVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 274 DAIVRLTREVVPGMIVTGMEVAEIDGAP 301
>dbj|BAC45141.1| putative thiamine biosynthetic enzyme [Oryza sativa (japonica
cultivar-group)]
Length = 352
Score = 163 bits (413), Expect = 2e-39
Identities = 90/141 (63%), Positives = 101/141 (70%), Gaps = 8/141 (5%)
Frame = -1
Query: 664 VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPG 485
V GVVTNWALVSMNHDTQSCMDPNVME+++VVSSCGHDGPFGATGVKRL+ IGMID+VPG
Sbjct: 196 VGGVVTNWALVSMNHDTQSCMDPNVMESRVVVSSCGHDGPFGATGVKRLQDIGMIDAVPG 255
Query: 484 MKALDMNTADRRYC*VHQ--------GGCTRNDSDWHGGS*N*WCPQHSGPTFGAMMISG 329
M+ALDMNTA+ + + G + D GPTFGAMMISG
Sbjct: 256 MRALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDG---------APRMGPTFGAMMISG 306
Query: 328 QKAAHLALKALGKNTCLIDGT 266
QKAAHLALKALG+ IDGT
Sbjct: 307 QKAAHLALKALGRPNA-IDGT 326
Score = 53.9 bits (128), Expect = 2e-06
Identities = 26/28 (92%), Positives = 26/28 (92%)
Frame = -2
Query: 453 DAIVRFTREVVPGMIVTGMEVAEIDGAP 370
D IVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 266 DEIVRLTREVVPGMIVTGMEVAEIDGAP 293
>sp|Q41739|TH42_MAIZE Thiazole biosynthetic enzyme 1-2, chloroplast precursor
gi|2130147|pir||S61420 thiamin biosynthesis protein
thi1-2 - maize gi|596080|gb|AAA96739.1| thiamine
biosynthetic enzyme
Length = 354
Score = 163 bits (413), Expect = 2e-39
Identities = 94/152 (61%), Positives = 104/152 (67%), Gaps = 12/152 (7%)
Frame = -1
Query: 664 VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPG 485
V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDGPFGATGVKRL+ IGMI +VPG
Sbjct: 200 VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLQDIGMISAVPG 259
Query: 484 MKALDMNTADRRYC*VHQ--------GGCTRNDSDWHGGS*N*WCPQHSGPTFGAMMISG 329
MKALDMN A+ + + G + D GPTFGAMMISG
Sbjct: 260 MKALDMNAAEDEIVRLTREVVPGMIVTGMEVAEIDG---------APRMGPTFGAMMISG 310
Query: 328 QKAAHLALKALGKNTCLIDGT----CEAVREE 245
QKAAHLALKALG+ +DGT A+REE
Sbjct: 311 QKAAHLALKALGRPNA-VDGTIPEVSPALREE 341
Score = 53.9 bits (128), Expect = 2e-06
Identities = 26/28 (92%), Positives = 26/28 (92%)
Frame = -2
Query: 453 DAIVRFTREVVPGMIVTGMEVAEIDGAP 370
D IVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 270 DEIVRLTREVVPGMIVTGMEVAEIDGAP 297
>sp|Q38709|THI4_ALNGL Thiazole biosynthetic enzyme, chloroplast precursor (AG6)
gi|1289204|emb|CAA66064.1| thaizole biosynthetic enzmye
[Alnus glutinosa]
Length = 352
Score = 161 bits (407), Expect = 8e-39
Identities = 91/141 (64%), Positives = 99/141 (69%), Gaps = 8/141 (5%)
Frame = -1
Query: 664 VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPG 485
V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDGPFGATGVK L+SIGMID+VPG
Sbjct: 200 VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKSLRSIGMIDTVPG 259
Query: 484 MKALDMNTADRRYC*VHQ--------GGCTRNDSDWHGGS*N*WCPQHSGPTFGAMMISG 329
MKALDMN A+ + + G + D GPTFGAMMISG
Sbjct: 260 MKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDG---------APRMGPTFGAMMISG 310
Query: 328 QKAAHLALKALGKNTCLIDGT 266
QKAAHLALKALG L DG+
Sbjct: 311 QKAAHLALKALGLPNAL-DGS 330
Score = 55.5 bits (132), Expect = 6e-07
Identities = 26/28 (92%), Positives = 27/28 (95%)
Frame = -2
Query: 453 DAIVRFTREVVPGMIVTGMEVAEIDGAP 370
DAIVR TRE+VPGMIVTGMEVAEIDGAP
Sbjct: 270 DAIVRLTREIVPGMIVTGMEVAEIDGAP 297
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 624,399,794
Number of Sequences: 1393205
Number of extensions: 14438951
Number of successful extensions: 46990
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 43744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 46798
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 28572683052
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
