>KMC004651A_C01 KMC004651A_c01
GACAAAATGAGTGGGGTGGAGTGTATATTTTGAATAAGAATGGGGTGGAGTGTTTTTCTA
GCAAACCTTGAGGGGGGTAAGTGTATTTTACTCAAAAAAGAAAGACACAAAGTTACACTG
ACAGTAGTAGCATATTTGGATTAACTTTTATTTCTCGAGAATCATAGCTTACTCCATCAA
ATTTTATTGTTGACTTTAAGAATCAACTGCGGAAGAATTTCGACGAACAATAACTTAGTG
GCTAGTTTTAGTGGTGTATCATCTTCCCAGTGAGTCCACAAGTGAGAGCTTCTCTAGGTA
TGTTCTTCACCTTATGAACAGCCTCGGCGAAAATCACACCCATTCCCATATGCAAATGCG
GCTCAATGTGACAATGGGAACGCCCAAACACCAGGGGTTATCCCGCCTTAAACCTCAAAG
CAGTCCAACCATAAGGGAAAATCACCGCGGTATTTCTCAACGGAGCGCGAGTCAAGTTGA
AACTCTTCTCATCAACACCAGGTCTAAACTTCCCTTCACCATACCCCAGAACCCAGAAAT
CATGCCCATGCAAATGCCATGGATGAATCTCACTCCCATTCCCATTCAACTGGTTAGCAT
TCTGCAGAATCACATCAACAACCTCGTTCAGCTGGAACATGTAAACCCCATTTCCGACCG
TCGTGTTGCGGAACACCGGACTCTGGAAAATGTTGT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004651A_C01 KMC004651A_c01
         (696 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAA75577.1| L-ascorbate oxidase [Medicago truncatula]             255  4e-67
gb|AAM94614.1| ascorbate oxidase precursor [Glycine max]              243  2e-63
gb|AAF20932.1|AF206722_1 ascorbate oxidase [Brassica juncea]          212  5e-54
gb|AAF20933.1|AF206723_1 ascorbate oxidase [Brassica juncea]          212  5e-54
ref|NP_680176.1| Expressed protein; protein id: At5g21105.1 [Ara...   208  7e-53

>emb|CAA75577.1| L-ascorbate oxidase [Medicago truncatula]
          Length = 569

 Score =  255 bits (652), Expect = 4e-67
 Identities = 121/141 (85%), Positives = 129/141 (90%)
 Frame = -3

Query: 694 NIFQSPVFRNTTVGNGVYMFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 515
           +IF++PV  NTT GNGVY FQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE
Sbjct: 425 DIFKNPVNPNTTTGNGVYTFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 484

Query: 514 GKFRPGVDEKSFNLTRAPLRNTAVIFPYGWTALRFKAG*PLVFGRSHCHIEPHLHMGMGV 335
           G+FRPGVDE+SFNLTRAPLRNTAVIFPYGWTALRFKA  P V+   HCHIEPHLHMGMGV
Sbjct: 485 GRFRPGVDERSFNLTRAPLRNTAVIFPYGWTALRFKADNPGVWA-FHCHIEPHLHMGMGV 543

Query: 334 IFAEAVHKVKNIPREALTCGL 272
           IFAEAVHKV+NIP+EAL C L
Sbjct: 544 IFAEAVHKVRNIPKEALKCVL 564

>gb|AAM94614.1| ascorbate oxidase precursor [Glycine max]
          Length = 429

 Score =  243 bits (620), Expect = 2e-63
 Identities = 118/146 (80%), Positives = 125/146 (84%)
 Frame = -3

Query: 694 NIFQSPVFRNTTVGNGVYMFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 515
           +IF  PV  N T+GNGVYMF LNEVVDVILQN+NQL+ NGSEIHPWHLHGHDFWVLGYGE
Sbjct: 280 DIFNPPVNPNATIGNGVYMFNLNEVVDVILQNSNQLSVNGSEIHPWHLHGHDFWVLGYGE 339

Query: 514 GKFRPGVDEKSFNLTRAPLRNTAVIFPYGWTALRFKAG*PLVFGRSHCHIEPHLHMGMGV 335
           GKF+ G DEK FNLT APLRNTAVIFPYGWTALRFKA  P V+   HCHIEPHLHMGMGV
Sbjct: 340 GKFKLG-DEKKFNLTHAPLRNTAVIFPYGWTALRFKADNPGVWA-FHCHIEPHLHMGMGV 397

Query: 334 IFAEAVHKVKNIPREALTCGLTGKMI 257
           IFAE VHKV  IPREALTCGLTGK +
Sbjct: 398 IFAEGVHKVGKIPREALTCGLTGKKL 423

>gb|AAF20932.1|AF206722_1 ascorbate oxidase [Brassica juncea]
          Length = 574

 Score =  212 bits (539), Expect = 5e-54
 Identities = 99/152 (65%), Positives = 116/152 (76%)
 Frame = -3

Query: 694 NIFQSPVFRNTTVGNGVYMFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 515
           +I   P  RNTT GNG+Y+F  N  VDVILQNAN L+ N SEIHPWHLHGHDFWVLGYGE
Sbjct: 424 DIMNPPRNRNTTKGNGIYVFPFNVTVDVILQNANGLDANASEIHPWHLHGHDFWVLGYGE 483

Query: 514 GKFRPGVDEKSFNLTRAPLRNTAVIFPYGWTALRFKAG*PLVFGRSHCHIEPHLHMGMGV 335
           GKFRPGVDEK++NL   PL+NT  +FPYGWTALRF    P V+   HCHIEPHLHMGMGV
Sbjct: 484 GKFRPGVDEKTYNLKNPPLKNTVALFPYGWTALRFVTDNPGVW-FFHCHIEPHLHMGMGV 542

Query: 334 IFAEAVHKVKNIPREALTCGLTGKMIHH*N*P 239
           +FAE ++++  IP EAL CGLT + + + N P
Sbjct: 543 VFAEGLNRIGKIPDEALGCGLTKQFLMNRNNP 574

>gb|AAF20933.1|AF206723_1 ascorbate oxidase [Brassica juncea]
          Length = 573

 Score =  212 bits (539), Expect = 5e-54
 Identities = 99/152 (65%), Positives = 116/152 (76%)
 Frame = -3

Query: 694 NIFQSPVFRNTTVGNGVYMFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 515
           +I   P  RNTT GNG+Y+F  N  VDVILQNAN L+ N SEIHPWHLHGHDFWVLGYGE
Sbjct: 423 DIMNPPRNRNTTKGNGIYVFPFNVTVDVILQNANGLDANASEIHPWHLHGHDFWVLGYGE 482

Query: 514 GKFRPGVDEKSFNLTRAPLRNTAVIFPYGWTALRFKAG*PLVFGRSHCHIEPHLHMGMGV 335
           GKFRPGVDEK++NL   PL+NT  +FPYGWTALRF    P V+   HCHIEPHLHMGMGV
Sbjct: 483 GKFRPGVDEKTYNLKNPPLKNTVALFPYGWTALRFVTDNPGVW-FFHCHIEPHLHMGMGV 541

Query: 334 IFAEAVHKVKNIPREALTCGLTGKMIHH*N*P 239
           +FAE ++++  IP EAL CGLT + + + N P
Sbjct: 542 VFAEGLNRIGKIPDEALGCGLTKQFLMNRNNP 573

>ref|NP_680176.1| Expressed protein; protein id: At5g21105.1 [Arabidopsis thaliana]
          Length = 571

 Score =  208 bits (529), Expect = 7e-53
 Identities = 93/150 (62%), Positives = 116/150 (77%)
 Frame = -3

Query: 694 NIFQSPVFRNTTVGNGVYMFQLNEVVDVILQNANQLNGNGSEIHPWHLHGHDFWVLGYGE 515
           +I   P F NTT GNG+Y+F  N  VDVI+QNAN L G  SEIHPWHLHGHDFWVLGYG+
Sbjct: 421 DIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGD 480

Query: 514 GKFRPGVDEKSFNLTRAPLRNTAVIFPYGWTALRFKAG*PLVFGRSHCHIEPHLHMGMGV 335
           GKF+PG+DEK++NL   PLRNTA+++PYGWTA+RF    P V+   HCHIEPHLHMGMGV
Sbjct: 481 GKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVW-FFHCHIEPHLHMGMGV 539

Query: 334 IFAEAVHKVKNIPREALTCGLTGKMIHH*N 245
           +FAE ++++  +P EAL CGLT + + + N
Sbjct: 540 VFAEGLNRIGKVPDEALGCGLTKQFLMNRN 569

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 635,321,684
Number of Sequences: 1393205
Number of extensions: 14416428
Number of successful extensions: 40913
Number of sequences better than 10.0: 339
Number of HSP's better than 10.0 without gapping: 36136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 39241
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 31684559424
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFBL022g09_f BP042375 1 329 2 SPD080f11_f BP050404 42 184 3 MR073c03_f BP081597 112 485 4 MF066b04_f BP031795 112 563 5 MF059e03_f BP031415 115 613 6 MPDL002g10_f AV776643 115 395 7 MF003h04_f BP028416 162 598 8 MF024f09_f BP029544 164 267 9 MF045d01_f BP030662 170 705 10 SPD086a09_f BP050835 171 646 11 MF004h02_f BP028465 171 615 12 MF066e10_f BP031825 171 653 13 MPD057c04_f AV773804 175 660 14 SPD028g08_f BP046246 187 713 15 MPD087c10_f AV775707 189 647 16 SPDL073c12_f BP056516 196 705 17 MFB067b04_f BP038838 196 757 18 SPD004a10_f BP044286 202 657 19 MFB062e03_f BP038510 204 735 20 MF085c03_f BP032764 205 751 21 MF090a01_f BP032999 219 759 22 MF090f09_f BP033034 221 740 23 MFBL018b12_f BP042150 261 620

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