>KMC003103A_C01 KMC003103A_c01
GGGCAGGAACAATTCACTTAAATATAAAATATATTCCCATAAATATATAATGAGTACAAA
GATGACAGAAGAGAATAACATAAAAAGGAAAGAAGACACACTAAAGGGAATACAGAGGAA
AGTGCAAAAGGCTCCCCTGTCACAACCAAAGGGACTTTAAAATCTACACTTATCCACAAT
CTGGAAGACCTAATTGAGAGGGAGATAAAGAAACCTTGCAGCAAATACAAGCAAGGCACA
AGCACTTCACATTATTTTCCTTCAAACTCTTCAATTATCTTCTTCTTTCCATCATGAAGT
TGAAGATAACTTAAACAGTGGGGTTACTCCTGAATGATCCAAGAGCCTTCACTGCACCTG
CTCTCAAGATGAACTGGTGGTAGAATGCTGCAATGGCTGCCCCAATGAATGGTCCTACCC
AGAAGATCCAATGGTCATCCCAGGGTTTAGATTGGTTGAAGATAACAGCAGCTCCAAAAC
TCCTAGCAGGGTTAATGCCAGTACCAGTGACTGGGATGGTGGCCAAGTGAACCATGAACA
CAGCAAATCCAATGGGAAGTGGAGCCAAAACCGGCACATGGGAATCTCTGGCACTTCTTT
TGGGATCAGTA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003103A_C01 KMC003103A_c01
         (611 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAC17529.1| aquaporin 2 [Samanea saman]                            197  9e-50
pir||T06434 plasma membrane intrinsic protein 1 - soybean gi|886...   193  2e-48
gb|AAL49751.1|AF452013_1 aquaporin-like protein [Petunia x hybrida]   192  4e-48
dbj|BAA32778.1| Plasma membrane aquaporin (PAQ2) [Raphanus sativus]   190  1e-47
dbj|BAB40143.1| plasma membrane intrinsic protein 2-2 [Pyrus com...   190  1e-47

>gb|AAC17529.1| aquaporin 2 [Samanea saman]
          Length = 287

 Score =  197 bits (501), Expect = 9e-50
 Identities = 94/99 (94%), Positives = 95/99 (95%)
 Frame = -2

Query: 610 TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSFGAAVIFNQSKPWDDH 431
           TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARS GAAVIFNQ K WDDH
Sbjct: 189 TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIFNQQKAWDDH 248

Query: 430 WIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPTV 314
           WIFWVGPFIGAAIAAFYHQFILRAGA KALGSFRSNP+V
Sbjct: 249 WIFWVGPFIGAAIAAFYHQFILRAGAAKALGSFRSNPSV 287

>pir||T06434 plasma membrane intrinsic protein 1 - soybean
           gi|886100|gb|AAA69490.1| putative water channel protein;
           plasmalemma intrinsic protein; similar to Arabidopsis
           Pip2a gene product, PIR Accession Number S44084
          Length = 285

 Score =  193 bits (490), Expect = 2e-48
 Identities = 90/99 (90%), Positives = 94/99 (94%)
 Frame = -2

Query: 610 TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSFGAAVIFNQSKPWDDH 431
           TDPKR+ARDSHVPVLAPLPIGFAVFMVHLA IPVTGTGINPARS GAAV++NQ K WDDH
Sbjct: 187 TDPKRNARDSHVPVLAPLPIGFAVFMVHLANIPVTGTGINPARSLGAAVMYNQQKAWDDH 246

Query: 430 WIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPTV 314
           WIFWVGPFIGAAIAAFYHQFILRAGA KALGSFRSNPT+
Sbjct: 247 WIFWVGPFIGAAIAAFYHQFILRAGAAKALGSFRSNPTI 285

>gb|AAL49751.1|AF452013_1 aquaporin-like protein [Petunia x hybrida]
          Length = 256

 Score =  192 bits (487), Expect = 4e-48
 Identities = 90/98 (91%), Positives = 93/98 (94%)
 Frame = -2

Query: 610 TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSFGAAVIFNQSKPWDDH 431
           TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARSFGAAVI+NQ K WDD 
Sbjct: 158 TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNQDKAWDDQ 217

Query: 430 WIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPT 317
           WIFWVGPFIGA IAA YHQF+LRAGAVKALGSFRSNPT
Sbjct: 218 WIFWVGPFIGALIAALYHQFVLRAGAVKALGSFRSNPT 255

>dbj|BAA32778.1| Plasma membrane aquaporin (PAQ2) [Raphanus sativus]
          Length = 287

 Score =  190 bits (482), Expect = 1e-47
 Identities = 89/99 (89%), Positives = 93/99 (93%)
 Frame = -2

Query: 610 TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSFGAAVIFNQSKPWDDH 431
           TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARSFGAAVIFN+SKPWDDH
Sbjct: 189 TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNESKPWDDH 248

Query: 430 WIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPTV 314
           WIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS   V
Sbjct: 249 WIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 287

>dbj|BAB40143.1| plasma membrane intrinsic protein 2-2 [Pyrus communis]
          Length = 287

 Score =  190 bits (482), Expect = 1e-47
 Identities = 90/99 (90%), Positives = 94/99 (94%)
 Frame = -2

Query: 610 TDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSFGAAVIFNQSKPWDDH 431
           TDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS GAAVI+N+ K WDD 
Sbjct: 189 TDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPITGTGINPARSLGAAVIYNKDKAWDDQ 248

Query: 430 WIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPTV 314
           WIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP V
Sbjct: 249 WIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 287

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 577,425,482
Number of Sequences: 1393205
Number of extensions: 13320868
Number of successful extensions: 49847
Number of sequences better than 10.0: 592
Number of HSP's better than 10.0 without gapping: 46795
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 49452
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24568846532
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF038d02_f BP030282 1 511 2 GNf049f08 BP071012 1 502 3 SPD009c10_f BP044702 2 623 4 MF062d08_f BP031582 2 411 5 GNf055b05 BP071444 3 423 6 MFB076c03_f BP039536 3 467 7 SPD010f04_f BP044810 12 211 8 MFB076a03_f BP039513 14 510 9 MWM181g11_f AV767523 15 636 10 MR075c07_f BP081758 15 420 11 MF055g03_f BP031211 15 544 12 SPD089d09_f BP051110 15 527 13 SPD029a12_f BP046269 15 507 14 SPD016a09_f BP045229 15 444 15 MR033b07_f BP078521 15 439 16 MR017h01_f BP077308 15 138 17 SPD041g10_f BP047288 16 472 18 SPD043e03_f BP047430 18 555 19 MF062c01_f BP031572 19 535 20 GNf020a03 BP068788 20 453 21 SPD031h06_f BP046500 20 609 22 MF017c05_f BP029137 20 487 23 MR060g03_f BP080625 20 433 24 MF010a08_f BP028725 20 266 25 MF063d05_f BP031639 32 590

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