>KMC000542A_C01 KMC000542A_c01
ataTGATAAAATTATTTTTTATTAAATATAAAAGTAAAGAATATAAAATTACAATAACAT
AAATTATTACTTAGTCCCGAATGACACCTCAAGTAGTAAGAGTTGATGACATGAGTTGAG
GAGGGTTCTTAACCCTGGCCGCAAGCACCTTATTGTGACATCTAGAGAAAACAATGGTCC
CATCAACCTAACACAAAATTTTTAAAACAAAGATTAAATCAAACAAAACTTACAAAGGAA
TAAGCACGGCATTTCCTTCTTTTTCTCTCTAACTCATGCCTATAATTTAAACCTTCCCTC
ATGATGTCTATAACATATAATTTACTTCCTGTCTGGTTACACTCATCTGGCGGCCAGCAC
CGGCTTTTCTCGCCGGAGGAGCAGTGTCCTCATCATCTGACTCGACATCTCCATACTGCC
ACATTCCACGCCGTTCCTTGCGTGCATCGTCCTGGAATTTTTGCAGACTATCAAGACCCA
CCTTTCTTTCTTTTCTGTCCCACCGGTTCCTTTTTTCCATCCTGGCAAGTCCTTCCTGTA
GCATGGCAGCGTTAACGCTGATCTC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC000542A_C01 KMC000542A_c01
         (565 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

dbj|BAC06184.1| 110 kDa 4SNc-Tudor domain protein [Pisum sativum]     113  2e-24
dbj|BAB32793.1| 110 kDa 4SNc-Tudor domain protein [Pisum sativum]     113  2e-24
ref|NP_196352.2| putative protein; protein id: At5g07350.1, supp...   104  7e-22
ref|NP_200986.1| 100 kDa coactivator - like protein; protein id:...   103  1e-21
pir||T49874 hypothetical protein T2I1.60 - Arabidopsis thaliana ...    99  5e-20

>dbj|BAC06184.1| 110 kDa 4SNc-Tudor domain protein [Pisum sativum]
          Length = 989

 Score =  113 bits (282), Expect = 2e-24
 Identities = 52/72 (72%), Positives = 60/72 (83%)
 Frame = -1

Query: 565  EISVNAAMLQEGLARMEKRNRWDRKERKVGLDSLQKFQDDARKERRGMWQYGDVESDDED 386
            EISVNAAMLQEGLARMEKRNRWD+  RK  LD+L+ FQ +AR  RRG+WQYGD++SDDED
Sbjct: 918  EISVNAAMLQEGLARMEKRNRWDKSARKQALDNLEMFQGEARTSRRGIWQYGDIQSDDED 977

Query: 385  TAPPARKAGAGR 350
            TAPP + AG  R
Sbjct: 978  TAPPRKPAGGRR 989

>dbj|BAB32793.1| 110 kDa 4SNc-Tudor domain protein [Pisum sativum]
          Length = 699

 Score =  113 bits (282), Expect = 2e-24
 Identities = 52/72 (72%), Positives = 60/72 (83%)
 Frame = -1

Query: 565 EISVNAAMLQEGLARMEKRNRWDRKERKVGLDSLQKFQDDARKERRGMWQYGDVESDDED 386
           EISVNAAMLQEGLARMEKRNRWD+  RK  LD+L+ FQ +AR  RRG+WQYGD++SDDED
Sbjct: 628 EISVNAAMLQEGLARMEKRNRWDKSARKQALDNLEMFQGEARTSRRGIWQYGDIQSDDED 687

Query: 385 TAPPARKAGAGR 350
           TAPP + AG  R
Sbjct: 688 TAPPRKPAGGRR 699

>ref|NP_196352.2| putative protein; protein id: At5g07350.1, supported by cDNA:
            gi_18086331 [Arabidopsis thaliana]
            gi|18086332|gb|AAL57629.1| AT5g07350/T2I1_60 [Arabidopsis
            thaliana]
          Length = 991

 Score =  104 bits (260), Expect = 7e-22
 Identities = 49/73 (67%), Positives = 60/73 (82%)
 Frame = -1

Query: 565  EISVNAAMLQEGLARMEKRNRWDRKERKVGLDSLQKFQDDARKERRGMWQYGDVESDDED 386
            EISVNAAMLQEG+ARMEKR +W  K ++  LD+L+KFQ++ARK R G+WQYGD+ESDDED
Sbjct: 920  EISVNAAMLQEGIARMEKRQKWGHKGKQAALDALEKFQEEARKSRIGIWQYGDIESDDED 979

Query: 385  TAPPARKAGAGRQ 347
            T  PARK   GR+
Sbjct: 980  TG-PARKPAGGRR 991

>ref|NP_200986.1| 100 kDa coactivator - like protein; protein id: At5g61780.1
            [Arabidopsis thaliana] gi|10176871|dbj|BAB10078.1|
            transcription factor-like protein [Arabidopsis thaliana]
            gi|25083258|gb|AAN72055.1| 100 kDa coactivator - like
            protein [Arabidopsis thaliana]
          Length = 985

 Score =  103 bits (258), Expect = 1e-21
 Identities = 47/71 (66%), Positives = 59/71 (82%)
 Frame = -1

Query: 565  EISVNAAMLQEGLARMEKRNRWDRKERKVGLDSLQKFQDDARKERRGMWQYGDVESDDED 386
            EISVNAAMLQEG+ARMEKR RW+ K+++  LD+L+KFQD+ARK R G+W+YGD++SDDED
Sbjct: 916  EISVNAAMLQEGIARMEKRRRWEPKDKQAALDALEKFQDEARKSRTGIWEYGDIQSDDED 975

Query: 385  TAPPARKAGAG 353
               P RK G G
Sbjct: 976  NV-PVRKPGRG 985

>pir||T49874 hypothetical protein T2I1.60 - Arabidopsis thaliana
            gi|7576173|emb|CAB87924.1| putative protein [Arabidopsis
            thaliana]
          Length = 1051

 Score = 98.6 bits (244), Expect = 5e-20
 Identities = 49/78 (62%), Positives = 60/78 (76%), Gaps = 5/78 (6%)
 Frame = -1

Query: 565  EISVNAAMLQ-----EGLARMEKRNRWDRKERKVGLDSLQKFQDDARKERRGMWQYGDVE 401
            EISVNAAMLQ     EG+ARMEKR +W  K ++  LD+L+KFQ++ARK R G+WQYGD+E
Sbjct: 920  EISVNAAMLQDDDEQEGIARMEKRQKWGHKGKQAALDALEKFQEEARKSRIGIWQYGDIE 979

Query: 400  SDDEDTAPPARKAGAGRQ 347
            SDDEDT  PARK   GR+
Sbjct: 980  SDDEDTG-PARKPAGGRR 996

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 494,918,874
Number of Sequences: 1393205
Number of extensions: 11031415
Number of successful extensions: 32226
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 31255
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32195
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20382500157
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 GNf064e02 BP072124 1 446 2 GNLf013h09 BP075593 28 545 3 GENLf053f07 BP065190 28 531 4 SPDL021b12_f BP053293 31 495 5 GNLf010b06 BP075380 33 438 6 GENLf025g02 BP063666 33 533 7 SPDL064e06_f BP055983 35 562 8 MRL023a07_f BP084879 35 492 9 SPDL074d05_f BP056577 36 602 10 MRL030h04_f BP085233 36 410 11 GENLf022e02 BP063508 37 532 12 MPDL026g09_f AV777821 48 500 13 MRL046a11_f BP085916 50 409 14 MFBL003f10_f BP041432 55 470 15 SPDL041d08_f BP054578 56 532 16 SPDL065f07_f BP056055 56 564 17 GENLf065d03 BP065854 57 556 18 SPDL040h07_f BP054549 78 375

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