Asamizu E, Nakamura Y, Sato S, Tabata S
A large scale analysis of cDNA in Arabidopsis thaliana: Generation of 12,028 non-redundant expressed sequence tags from normalized and size-selected cDNA libraries
DNA Research, 7, 175-180 (2000)

Figures and Tables

Table 1 Number of 5'-end and 3'-end ESTs generated from cDNA libraries of five different tissues.
Tissue Library type Number of
5' end ESTs
Number of
3' end ESTs
Aboveground organs Normalized 4996 6863
Size-selected 2172 1753
Flower buds Normalized ND 5827
Roots Normalized 5798 8505
Size-selected 245 3161
Green siliques Normalized ND 11843
Size-selected ND 909
Liquid-cultured seedlings Normalized 815 346
Total   14026 39207
    ND: Not determined  

Table 2 Classification of 3'-end ESTs by similarity search against the non-redundant protein database.
Similarity Number of
Number of
non-redundant ESTs
Genes of known functiona) 24892 4816
Hypothetical genesb) 5071 1864
No similarityc) 9244 5348
Total 39207 12028
a) showed similarity to genes of known function,
b) showed similarity to hypothetical genes that have no definition of function,
c) showed no similarity

Table 3 Classification of the non-redundant EST groups with similarity to known protein genes by their functional categories
Functional categories Number of non-
redundant groups
Energy metabolism 538
Regulatory functions 483
Cellular structure, organization and biogenesis 416
Protein fate 369
Signal transduction 360
Protein synthesis 328
Transport and binding proteins 319
Cellular processes 218
Secondary metabolism 209
Growth and development 184
Fatty acid and phospholipid metabolism 132
Amino-acid biosynthesis 125
Environmental response 113
Pathogen responses 99
DNA metabolism 89
General transcription 76
Purines, pyrimidines, nucleosides, and nucleotides 68
Central intermediary metabolism 53
Biosynthesis of cofactors, prosthetic groups, and carriers 52
Other categories 23
Unclassified 562
Total 4816

Figure 1 The proportion of EST groups identified only in one of the five tissues and those identified in two to five tissues. The proportion of each category are given as percentages and indicated by color codes.

Arabidopsis thaliana
Kazusa DNA Research Institute
Department of Plant Gene Research