##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AclI AflII AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspI AviII BcgI BclI
BfrI BsaBI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DraI
DrdI Eco47III EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SexAI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2587 GGTACC G^GTACC
AccI 2241 GTCTAC GT^[AC][GT]AC
AccIII 1600 TCCGGA T^CCGGA
AcsI 2912 GAATTC [GA]^AATT[CT]
ApoI 2912 GAATTC [GA]^AATT[CT]
Asp718I 2587 GGTACC G^GTACC
BamHI 626 GGATCC G^GATCC
BbeI 1365 GGCGCC GGCGC^C
BbrPI 1275 CACGTG CAC^GTG
BbsI 1006 GAAGAC GAAGAC(2/6)
BciVI 1479 GGATAC GTATCC(6/5)
BglII 2056 AGATCT A^GATCT
BlpI 2799 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2799 GCTGAGC GC^T[ACGT]AGC
BpuAI 1006 GAAGAC GAAGAC(2/6)
BsaAI 1275 CACGTG [CT]AC^GT[GA]
BseAI 1600 TCCGGA T^CCGGA
BsmBI 69 CGTCTC CGTCTC(1/5)
BspEI 1600 TCCGGA T^CCGGA
BspHI 720 TCATGA T^CATGA
BsrBI 2368 CCGCTC CCGCTC(-3/-3)
BstBI 614 TTCGAA TT^CGAA
CelII 2799 GCTGAGC GC^T[ACGT]AGC
EcoRI 2912 GAATTC G^AATTC
FseI 1457 GGCCGGCC GGCCGG^CC
KasI 1365 GGCGCC G^GCGCC
KpnI 2587 GGTACC GGTAC^C
MroI 1600 TCCGGA T^CCGGA
MseI 3076 TTAA T^TAA
NarI 1365 GGCGCC GG^CGCC
NspV 614 TTCGAA TT^CGAA
PmaCI 1275 CACGTG CAC^GTG
PmlI 1275 CACGTG CAC^GTG
RcaI 720 TCATGA T^CATGA
SbfI 1778 CCTGCAGG CCTGCA^GG
ScaI 1980 AGTACT AGT^ACT
SfoI 1365 GGCGCC GGC^GCC
SfuI 614 TTCGAA TT^CGAA
SmlI 3019 CTCAAG C^T[CT][GA]AG
Sse8387I 1778 CCTGCAGG CCTGCA^GG
TfiI 612 GATTC G^A[AT]TC
Tru9I 3076 TTAA T^TAA
Tsp509I 2913 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 67 GACGTC GACGT^C
1596 GACGTC
AfaI 1981 GTAC GT^AC
2588 GTAC
BbvCI 1907 CCTCAGC CCTCAGC(-5/-2)
2537 CCTCAGC
BsaWI 1600 TCCGGA [AT]^CCGG[AT]
3056 ACCGGA
BstAPI 445 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2226 GCACATTGTGC
BstEII 1583 GGTGACC G^GT[ACGT]ACC
2896 GGTGACC
BstPI 1583 GGTGACC G^GT[ACGT]ACC
2896 GGTGACC
BstXI 395 CCAGTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2158 CCACATCGCTGG
DraIII 2227 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
3084 CACTGTGTG
EcoO65I 1583 GGTGACC G^GT[ACGT]ACC
2896 GGTGACC
KspI 572 CCGCGG CCGC^GG
1796 CCGCGG
MslI 2859 CATGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2880 CACAGGTGTG
NcoI 699 CCATGG C^CATGG
2437 CCATGG
PflMI 165 CCAACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2252 CCATTACATGG
PleI 1821 GACTC GAGTC(4/5)
231 GAGTC
PvuII 554 CAGCTG CAG^CTG
2650 CAGCTG
RsaI 1981 GTAC GT^AC
2588 GTAC
SacII 572 CCGCGG CCGC^GG
1796 CCGCGG
SfiI 982 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2519 GGCCGCCGTGGCC
SmaI 341 CCCGGG CCC^GGG
2030 CCCGGG
StuI 1783 AGGCCT AGG^CCT
2043 AGGCCT
Van91I 165 CCAACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2252 CCATTACATGG
XcmI 1410 CCAGCAGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1570 CCACCCCCAGCCTGG
XmaCI 341 CCCGGG C^CCGGG
2030 CCCGGG
XmaI 341 CCCGGG C^CCGGG
2030 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AhdI AluI
AlwI AlwNI AocI ApaI AspEI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbvI BcnI BfaI
BglI BlnI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PpuMI PstI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII