##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AcyI AscI Asp700I AviII
BamHI BbeI BlpI Bpu1102I BsaHI
BsiWI BspDI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI EagI EclXI Eco52I
EcoO65I EcoT22I FseI FspI Hin1I
KasI KspI MfeI MunI NaeI
NarI NdeI NgoMIV NotI NsiI
NspV PacI PmeI PshAI RsrII
SacII SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1661 GGTACC G^GTACC
AccI 4043 GTCGAC GT^[AC][GT]AC
AccIII 2782 TCCGGA T^CCGGA
AflII 5166 CTTAAG C^TTAAG
AgeI 3297 ACCGGT A^CCGGT
Alw44I 4357 GTGCAC G^TGCAC
Aor51HI 2482 AGCGCT AGC^GCT
ApaLI 4357 GTGCAC G^TGCAC
AseI 1878 ATTAAT AT^TAAT
AsnI 1878 ATTAAT AT^TAAT
Asp718I 1661 GGTACC G^GTACC
AspI 4845 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2164 CCTAGG C^CTAGG
BbrPI 63 CACGTG CAC^GTG
BbvCI 4936 CCTCAGC CCTCAGC(-5/-2)
BciVI 5274 GGATAC GTATCC(6/5)
BclI 2710 TGATCA T^GATCA
BfrI 5166 CTTAAG C^TTAAG
BlnI 2164 CCTAGG C^CTAGG
BsaBI 5509 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2782 TCCGGA T^CCGGA
BsiEI 4159 CGATCG CG[GA][CT]^CG
BsmBI 3052 GAGACG CGTCTC(1/5)
BspEI 2782 TCCGGA T^CCGGA
BspLU11I 2004 ACATGT A^CATGT
BsrDI 2508 GCAATG GCAATG(2/0)
BstAPI 841 GCATATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1423 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2527 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2482 AGCGCT AGC^GCT
EcoRV 5513 GATATC GAT^ATC
FbaI 2710 TGATCA T^GATCA
HaeII 2482 AGCGCT [GA]GCGC^[CT]
HindIII 196 AAGCTT A^AGCTT
HpaI 5027 GTTAAC GTT^AAC
KpnI 1661 GGTACC GGTAC^C
MamI 5509 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2782 TCCGGA T^CCGGA
NcoI 2969 CCATGG C^CATGG
NheI 3751 GCTAGC G^CTAGC
NruI 3105 TCGCGA TCG^CGA
PflFI 4845 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3297 ACCGGT A^CCGGT
PmaCI 63 CACGTG CAC^GTG
PmlI 63 CACGTG CAC^GTG
PshBI 1878 ATTAAT AT^TAAT
PvuI 4159 CGATCG CGAT^CG
SalI 4043 GTCGAC G^TCGAC
SapI 3851 GAAGAGC GCTCTTC(1/4)
StuI 3889 AGGCCT AGG^CCT
Tth111I 4845 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3416 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AclI 2664 AACGTT AA^CGTT
2775 AACGTT
AlwI 1025 GATCC GGATC(4/5)
5061 GATCC
AocI 352 CCTCAGG CC^T[ACGT]AGG
3570 CCTGAGG
BcgI 2271 GCAGCTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3096 GCAGTCATGTCG
BsaAI 63 CACGTG [CT]AC^GT[GA]
3501 CACGTA
BsmI 1648 GAATGC GAATGC(1/-1)
2938 GAATGC
Bsp1407I 2031 TGTACA T^GTACA
5470 TGTACA
BspHI 5057 TCATGA T^CATGA
5378 TCATGA
BspMI 1227 ACCTGC ACCTGC(4/8)
2422 GCAGGT
BsrFI 276 GCCGGT [GA]^CCGG[CT]
3297 ACCGGT
BsrGI 2031 TGTACA T^GTACA
5470 TGTACA
BssSI 3655 CACGAG CACGAG(-5/-1)
202 CTCGTG
Bsu36I 352 CCTCAGG CC^T[ACGT]AGG
3570 CCTGAGG
CfoI 476 GCGC GCG^C
2483 GCGC
Cfr10I 276 GCCGGT [GA]^CCGG[CT]
3297 ACCGGT
DsaI 1500 CCGTGG C^C[GA][CT]GG
2969 CCATGG
Eco81I 352 CCTCAGG CC^T[ACGT]AGG
3570 CCTGAGG
EcoRI 4820 GAATTC G^AATTC
5130 GAATTC
FauI 580 GCGGG CCCGC(4/6)
2198 GCGGG
HhaI 476 GCGC GCG^C
2483 GCGC
HinP1I 476 GCGC G^CGC
2483 GCGC
MluI 2082 ACGCGT A^CGCGT
4978 ACGCGT
PaeR7I 3608 CTCGAG C^TCGAG
3800 CTCGAG
Psp1406I 2664 AACGTT AA^CGTT
2775 AACGTT
RcaI 5057 TCATGA T^CATGA
5378 TCATGA
SacI 322 GAGCTC GAGCT^C
2887 GAGCTC
SmaI 3585 CCCGGG CCC^GGG
3918 CCCGGG
SphI 3864 GCATGC GCATG^C
4108 GCATGC
SspBI 2031 TGTACA T^GTACA
5470 TGTACA
SspI 1597 AATATT AAT^ATT
5340 AATATT
XhoI 3608 CTCGAG C^TCGAG
3800 CTCGAG
XmaCI 3585 CCCGGG C^CCGGG
3918 CCCGGG
XmaI 3585 CCCGGG C^CCGGG
3918 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AhdI AluI AlwNI ApaI ApoI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII