##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI AhdI AscI AspEI AviII
BalI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstBI BstUI BstXI
BstZ17I ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FseI FspI HpaI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1348 GGTACC G^GTACC
AccI 3715 GTAGAC GT^[AC][GT]AC
AflII 4380 CTTAAG C^TTAAG
Aor51HI 3842 AGCGCT AGC^GCT
Asp700I 358 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1348 GGTACC G^GTACC
AspI 2474 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 416 CCTAGG C^CTAGG
BamHI 3587 GGATCC G^GATCC
BbeI 4350 GGCGCC GGCGC^C
BbrPI 2889 CACGTG CAC^GTG
BbvCI 2256 GCTGAGG CCTCAGC(-5/-2)
BcgI 3266 GCATTTCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3752 GTATCC GTATCC(6/5)
BfrI 4380 CTTAAG C^TTAAG
BlnI 416 CCTAGG C^CTAGG
BsaAI 2889 CACGTG [CT]AC^GT[GA]
BsaBI 2672 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 4424 TGTACA T^GTACA
BspHI 861 TCATGA T^CATGA
BsrFI 2796 GCCGGC [GA]^CCGG[CT]
BsrGI 4424 TGTACA T^GTACA
BssSI 1113 CACGAG CACGAG(-5/-1)
BstEII 4334 GGTAACC G^GT[ACGT]ACC
BstPI 4334 GGTAACC G^GT[ACGT]ACC
Cfr10I 2796 GCCGGC [GA]^CCGG[CT]
DraIII 2613 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1373 GACCGTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3850 TGGCCG [CT]^GGCC[GA]
Eco47III 3842 AGCGCT AGC^GCT
EcoO65I 4334 GGTAACC G^GT[ACGT]ACC
HgaI 3405 GCGTC GACGC(5/10)
HincII 1684 GTCAAC GT[CT]^[GA]AC
HindII 1684 GTCAAC GT[CT]^[GA]AC
KasI 4350 GGCGCC G^GCGCC
KpnI 1348 GGTACC GGTAC^C
MamI 2672 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2796 GCCGGC GCC^GGC
NarI 4350 GGCGCC GG^CGCC
NgoMIV 2796 GCCGGC G^CCGGC
PaeR7I 422 CTCGAG C^TCGAG
PflFI 2474 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2889 CACGTG CAC^GTG
PmlI 2889 CACGTG CAC^GTG
RcaI 861 TCATGA T^CATGA
SacI 130 GAGCTC GAGCT^C
ScaI 2657 AGTACT AGT^ACT
SfoI 4350 GGCGCC GGC^GCC
SspBI 4424 TGTACA T^GTACA
Tth111I 2474 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2792 CCAGGCCGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 422 CTCGAG C^TCGAG
XmnI 358 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 3404 GGCGTC G[GA]^CG[CT]C
4350 GGCGCC
Alw44I 771 GTGCAC G^TGCAC
4371 GTGCAC
ApaI 1032 GGGCCC GGGCC^C
3680 GGGCCC
ApaLI 771 GTGCAC G^TGCAC
4371 GTGCAC
AseI 3359 ATTAAT AT^TAAT
4156 ATTAAT
AsnI 3359 ATTAAT AT^TAAT
4156 ATTAAT
BglI 107 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
945 GCCTCCCTGGC
BglII 2444 AGATCT A^GATCT
4542 AGATCT
BsaHI 3404 GGCGTC G[GA]^CG[CT]C
4350 GGCGCC
BsaWI 3584 ACCGGA [AT]^CCGG[AT]
3933 ACCGGA
BstAPI 2732 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2800 GCAGTGGCTGC
DraI 2581 TTTAAA TTT^AAA
4516 TTTAAA
FauI 1443 CCCGC CCCGC(4/6)
3591 CCCGC
Hin1I 3404 GGCGTC G[GA]^CG[CT]C
4350 GGCGCC
HindIII 2076 AAGCTT A^AGCTT
3518 AAGCTT
NspI 628 GCATGT [GA]CATG^[CT]
1300 GCATGT
PshBI 3359 ATTAAT AT^TAAT
4156 ATTAAT
PstI 3038 CTGCAG CTGCA^G
3420 CTGCAG
SspI 3349 AATATT AAT^ATT
3383 AATATT
StuI 1627 AGGCCT AGG^CCT
2869 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BclI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII