BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146561.3 + phase: 0
(262 letters)
Database: uniref100
2,790,947 sequences; 848,049,833 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef100_Q9SZV0 Hypothetical protein F6G17.160 [Arabidopsis tha... 370 e-101
UniRef100_Q8S1Z0 Hypothetical protein P0468B07.33 [Oryza sativa] 317 2e-85
UniRef100_UPI000034B21C UPI000034B21C UniRef100 entry 44 0.004
UniRef100_UPI000025C112 UPI000025C112 UniRef100 entry 44 0.005
UniRef100_UPI00002E51AF UPI00002E51AF UniRef100 entry 43 0.007
UniRef100_UPI000032AB7B UPI000032AB7B UniRef100 entry 43 0.009
UniRef100_UPI000027E55C UPI000027E55C UniRef100 entry 43 0.009
UniRef100_Q732M1 Ribonuclease III [Bacillus cereus] 42 0.016
UniRef100_Q9CHD0 Ribonuclease III [Lactococcus lactis] 42 0.016
UniRef100_Q819V8 Ribonuclease III [Bacillus cereus] 42 0.016
UniRef100_Q81WI8 Ribonuclease III [Bacillus anthracis] 42 0.016
UniRef100_Q636H7 Ribonuclease III [Bacillus cereus] 42 0.021
UniRef100_Q6BCJ9 Dicer-related RNase III protein Dcr2p [Tetrahym... 42 0.021
UniRef100_UPI00002C9A18 UPI00002C9A18 UniRef100 entry 41 0.027
UniRef100_UPI0000293917 UPI0000293917 UniRef100 entry 41 0.027
UniRef100_UPI00002694E9 UPI00002694E9 UniRef100 entry 41 0.027
UniRef100_Q65JQ5 Rnc [Bacillus licheniformis] 41 0.027
UniRef100_P51833 Ribonuclease III [Bacillus subtilis] 41 0.027
UniRef100_Q9Z5U2 Ribonuclease III [Zymomonas mobilis] 41 0.035
UniRef100_Q5XDA6 Ribonuclease III [Streptococcus pyogenes] 40 0.046
>UniRef100_Q9SZV0 Hypothetical protein F6G17.160 [Arabidopsis thaliana]
Length = 537
Score = 370 bits (949), Expect = e-101
Identities = 185/253 (73%), Positives = 208/253 (82%), Gaps = 1/253 (0%)
Query: 10 SPKPTNFSFSSSFSPFPIQILIKNTKSRNYPLHVLAVAIDPTKDFPRNNNPQRLLKELAE 69
S SFSSS S FP + TKSR L + A T+ PR++ PQRLLKELA+
Sbjct: 17 SSSAPEISFSSSISQFPSKTQSILTKSRFQNLRICASVTAETQGLPRDS-PQRLLKELAQ 75
Query: 70 RKKITNPKSKSPPRRYILKPPLDDKKLAERFLNSPQLSLKSFPLLSSCLPSLRLNNADKL 129
RK T PK K PP+R+IL+PPLDDKKLAERFLNSPQLSLKSFPLLSSCLPS +LNNADK
Sbjct: 76 RKTATGPKKKVPPKRFILRPPLDDKKLAERFLNSPQLSLKSFPLLSSCLPSSKLNNADKT 135
Query: 130 WIDEYLLEAKQALGYSLEPSETLEDDNPAKQFDTLLYLAFQHPSCERTKQKHVRSGHSRL 189
WIDEYLLE KQALGYSLEPSE+L DDNPAK FDTLLYLAFQHPSC+R + +HV++GHSRL
Sbjct: 136 WIDEYLLEVKQALGYSLEPSESLGDDNPAKHFDTLLYLAFQHPSCDRARARHVKNGHSRL 195
Query: 190 FFLGQYVLELALAEFFLQRYPRELPGPMRERVYGLIGKENLPKWIKAASLQNLIFPYDNM 249
+FLGQYVLELAL EFFLQRYPRE PGPMRERV+ LIGK LPKWIKAASLQNLIFPYD+M
Sbjct: 196 WFLGQYVLELALTEFFLQRYPRESPGPMRERVFALIGKRYLPKWIKAASLQNLIFPYDDM 255
Query: 250 DKIVRRDREGPVK 262
DK++R++RE PVK
Sbjct: 256 DKLIRKEREPPVK 268
Score = 43.9 bits (102), Expect = 0.004
Identities = 37/144 (25%), Positives = 72/144 (49%), Gaps = 7/144 (4%)
Query: 104 PQLSLKSFP-LLSSCLPSLRLNNADKLWIDEYLLEAKQALGYSLEPSETLEDDNPAKQFD 162
P+ +L + P L +C+P +W + + Q LGY L ++ +++ A+ +
Sbjct: 338 PEDALFAHPRLFRACVPPGMHRFRGNIWDFDSKPKVMQTLGYPLTMNDRIKEITEARNIE 397
Query: 163 TLL--YLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGP-MRE 219
L L F HPS + K +H R RL ++GQ + ++A+AE L ++ + PG ++E
Sbjct: 398 LGLGLQLCFLHPS--KHKFEHPRFCFERLEYVGQKIQDIAMAERLLMKH-LDAPGKWLQE 454
Query: 220 RVYGLIGKENLPKWIKAASLQNLI 243
+ L+ + ++++ L N I
Sbjct: 455 KHRRLLMNKFCGRYLREKRLHNFI 478
>UniRef100_Q8S1Z0 Hypothetical protein P0468B07.33 [Oryza sativa]
Length = 533
Score = 317 bits (812), Expect = 2e-85
Identities = 163/256 (63%), Positives = 194/256 (75%), Gaps = 17/256 (6%)
Query: 21 SFSPFPIQILIKNTKSRNYPLHVLAVAIDPTKDFPRN--------NNPQRLLKELAERKK 72
+ P P+ + + R + VLAVA D T P + N+P RLL+ELAERKK
Sbjct: 12 ALGPLPLPLPAARRRRR---VRVLAVAADHTPPPPPSPSSPPEPANSPSRLLRELAERKK 68
Query: 73 ITNPKSKSPPRRYILKPPLDDKKLAERFLNSPQLSLKSFPLLSSCLPSLRLNNADKLWID 132
+PK K PPRR+ILKPPLDD++L +RFL+SPQLSLK+ PLLSSCLPS L+ AD+ W+D
Sbjct: 69 AVSPKKKHPPRRFILKPPLDDERLTQRFLSSPQLSLKALPLLSSCLPSAPLSAADRTWMD 128
Query: 133 EYLLEAKQALGYSLEPSETLEDDN----PAKQFDTLLYLAFQH--PSCERTKQKHVRSGH 186
EYLLEAKQALGY L PSETL D + PA+ FD LLYLAFQH SCERT+ +HVRSGH
Sbjct: 129 EYLLEAKQALGYPLAPSETLGDGDDDGCPARHFDVLLYLAFQHLDTSCERTRTRHVRSGH 188
Query: 187 SRLFFLGQYVLELALAEFFLQRYPRELPGPMRERVYGLIGKENLPKWIKAASLQNLIFPY 246
SRL+FLGQYVLELA EFFLQRYPRE PGPMRERV+ LIGK +PKWIKAASL NL+FPY
Sbjct: 189 SRLWFLGQYVLELAFCEFFLQRYPRESPGPMRERVFALIGKRAIPKWIKAASLHNLVFPY 248
Query: 247 DNMDKIVRRDREGPVK 262
D++DK++R+DRE P K
Sbjct: 249 DDLDKMIRKDREPPAK 264
Score = 36.2 bits (82), Expect = 0.87
Identities = 26/99 (26%), Positives = 46/99 (46%), Gaps = 4/99 (4%)
Query: 113 LLSSCLPSLRLNNADKLWIDEYLLEAKQALGYSLEPSETLEDDNPAKQFDTLL--YLAFQ 170
L +C+P +W + + LGY L ++ + + A+ + L L F
Sbjct: 344 LFRACVPPGMHRFRGNIWDFDNRPKVMNTLGYPLPMNDRIPEITEARNIELGLGLQLCFL 403
Query: 171 HPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRY 209
HPS + K +H R RL ++GQ + +L +AE L ++
Sbjct: 404 HPS--KHKFEHPRFCLERLEYVGQKIQDLVMAERLLMKH 440
>UniRef100_UPI000034B21C UPI000034B21C UniRef100 entry
Length = 125
Score = 43.9 bits (102), Expect = 0.004
Identities = 27/87 (31%), Positives = 41/87 (47%), Gaps = 6/87 (6%)
Query: 164 LLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERVYG 223
LL A HPS +R+ + + RL FLG VL L +AE + +P E G + R+
Sbjct: 17 LLLEALSHPSLDRS------ASYQRLEFLGDRVLGLVIAEALFKEFPDEAEGALNNRLSA 70
Query: 224 LIGKENLPKWIKAASLQNLIFPYDNMD 250
L+ +E + + L LI + D
Sbjct: 71 LVRRETVAEVALGLGLDQLIILSEGAD 97
>UniRef100_UPI000025C112 UPI000025C112 UniRef100 entry
Length = 198
Score = 43.5 bits (101), Expect = 0.005
Identities = 23/73 (31%), Positives = 39/73 (52%)
Query: 171 HPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERVYGLIGKENL 230
H S +++ K ++ + RL FLG VL L L+E+F + +P G + +ENL
Sbjct: 1 HSSYQKSNLKIKKNNYERLEFLGDRVLGLVLSEYFFKTFPTANEGILDNYFQKYANQENL 60
Query: 231 PKWIKAASLQNLI 243
K+ K+ +L N +
Sbjct: 61 FKYAKSINLNNFL 73
>UniRef100_UPI00002E51AF UPI00002E51AF UniRef100 entry
Length = 143
Score = 43.1 bits (100), Expect = 0.007
Identities = 23/71 (32%), Positives = 38/71 (53%)
Query: 160 QFDTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRE 219
Q D LL+LA H S K K + + +L FLG VL++A++E + R+P G + +
Sbjct: 21 QDDELLHLALTHKSFTEFKSKSGQKNNEKLEFLGDAVLDMAISELLMDRFPELDEGGLSK 80
Query: 220 RVYGLIGKENL 230
L+ ++ L
Sbjct: 81 FRASLVNEDGL 91
>UniRef100_UPI000032AB7B UPI000032AB7B UniRef100 entry
Length = 149
Score = 42.7 bits (99), Expect = 0.009
Identities = 27/85 (31%), Positives = 42/85 (48%), Gaps = 3/85 (3%)
Query: 164 LLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERVYG 223
+L LA H S +K+ + RL FLG VL L +AE +++P E G + +R
Sbjct: 27 ILKLALTHSSFV---EKNSNKNYERLEFLGDRVLGLVIAEMLFKKFPNEKEGELSKRFAY 83
Query: 224 LIGKENLPKWIKAASLQNLIFPYDN 248
L+ K+ L K L+++I N
Sbjct: 84 LVNKKTLIKVADLLGLRDVIITAKN 108
>UniRef100_UPI000027E55C UPI000027E55C UniRef100 entry
Length = 145
Score = 42.7 bits (99), Expect = 0.009
Identities = 26/80 (32%), Positives = 38/80 (47%)
Query: 164 LLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERVYG 223
LL A H S +R K ++ + RL FLG VL L L+E+F + YP G +
Sbjct: 19 LLEEAITHSSFQRNNLKIKKNNYERLEFLGDRVLGLVLSEYFFKTYPNANEGVLDNYFQR 78
Query: 224 LIGKENLPKWIKAASLQNLI 243
+ENL + K +L +
Sbjct: 79 HANQENLFNYAKKINLSTYL 98
>UniRef100_Q732M1 Ribonuclease III [Bacillus cereus]
Length = 245
Score = 42.0 bits (97), Expect = 0.016
Identities = 23/82 (28%), Positives = 41/82 (49%)
Query: 162 DTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERV 221
+ LL AF H S +K + RL FLG VLEL ++++ Q+YP G + +
Sbjct: 33 EKLLIQAFTHSSYVNEHRKKPHEDNERLEFLGDAVLELTVSQYLFQKYPTMSEGELTKLR 92
Query: 222 YGLIGKENLPKWIKAASLQNLI 243
++ + +L ++ S +L+
Sbjct: 93 AAIVCEPSLVRFANELSFGSLV 114
>UniRef100_Q9CHD0 Ribonuclease III [Lactococcus lactis]
Length = 231
Score = 42.0 bits (97), Expect = 0.016
Identities = 24/97 (24%), Positives = 46/97 (46%)
Query: 162 DTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERV 221
+ LL AF H S ++ + + RL FLG L L ++++ + YP +L G + +
Sbjct: 19 EDLLKTAFTHSSFTNEERLPKIANNERLEFLGDVALSLVISDYLYRTYPEKLEGELSKMR 78
Query: 222 YGLIGKENLPKWIKAASLQNLIFPYDNMDKIVRRDRE 258
++ E+L + ++ + +K+ RDRE
Sbjct: 79 SSIVRTESLANFSRSCGFGEFLRLGHGEEKMGGRDRE 115
>UniRef100_Q819V8 Ribonuclease III [Bacillus cereus]
Length = 245
Score = 42.0 bits (97), Expect = 0.016
Identities = 23/82 (28%), Positives = 41/82 (49%)
Query: 162 DTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERV 221
+ LL AF H S +K + RL FLG VLEL ++++ Q+YP G + +
Sbjct: 33 EKLLIQAFTHSSYVNEHRKKPHEDNERLEFLGDAVLELTVSQYLFQKYPTMSEGELTKLR 92
Query: 222 YGLIGKENLPKWIKAASLQNLI 243
++ + +L ++ S +L+
Sbjct: 93 AAIVCEPSLVRFANELSFGSLV 114
>UniRef100_Q81WI8 Ribonuclease III [Bacillus anthracis]
Length = 245
Score = 42.0 bits (97), Expect = 0.016
Identities = 23/82 (28%), Positives = 41/82 (49%)
Query: 162 DTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERV 221
+ LL AF H S +K + RL FLG VLEL ++++ Q+YP G + +
Sbjct: 33 EKLLIQAFTHSSYVNEHRKKPHEDNERLEFLGDAVLELTVSQYLFQKYPTMSEGELTKLR 92
Query: 222 YGLIGKENLPKWIKAASLQNLI 243
++ + +L ++ S +L+
Sbjct: 93 AAIVCEPSLVRFANELSFGSLV 114
>UniRef100_Q636H7 Ribonuclease III [Bacillus cereus]
Length = 245
Score = 41.6 bits (96), Expect = 0.021
Identities = 23/82 (28%), Positives = 40/82 (48%)
Query: 162 DTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERV 221
+ LL AF H S +K + RL FLG VLEL ++++ Q+YP G + +
Sbjct: 33 EKLLIQAFTHSSYVNEHRKKPHEDNERLEFLGDAVLELTVSQYLFQKYPTMSEGELTKLR 92
Query: 222 YGLIGKENLPKWIKAASLQNLI 243
++ + +L ++ S L+
Sbjct: 93 AAIVCEPSLVRFANELSFGGLV 114
>UniRef100_Q6BCJ9 Dicer-related RNase III protein Dcr2p [Tetrahymena thermophila]
Length = 1838
Score = 41.6 bits (96), Expect = 0.021
Identities = 32/124 (25%), Positives = 55/124 (43%), Gaps = 9/124 (7%)
Query: 107 SLKSFPLLSSCLPSLRLNNADKLWIDEYLLEAKQALGYSLEPSETLEDDNPAKQFDTLLY 166
+L S L +PSL+ N +KL ++ K+ G + E + + +N K
Sbjct: 1552 TLPSIESLCIQIPSLKNTNLNKLHQINNAIQKKENQG-TFEKIDQISKENQNK------- 1603
Query: 167 LAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERVYGLIG 226
+Q S E K+ + RL FLG VL+L + E+ ++P PG + + L+
Sbjct: 1604 -IYQKDSNEWENLKNYNLCNQRLEFLGDAVLDLIIVEYLFNKFPNSDPGELTQMKTSLVQ 1662
Query: 227 KENL 230
+ L
Sbjct: 1663 NKTL 1666
>UniRef100_UPI00002C9A18 UPI00002C9A18 UniRef100 entry
Length = 116
Score = 41.2 bits (95), Expect = 0.027
Identities = 22/71 (30%), Positives = 38/71 (52%)
Query: 160 QFDTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRE 219
Q + LL+LA H S K K + + +L FLG VL++A++E + R+P G + +
Sbjct: 21 QNEELLHLALTHKSFTEFKSKSGQRNNEKLEFLGDAVLDMAISELLMDRFPELDEGGLSK 80
Query: 220 RVYGLIGKENL 230
L+ ++ L
Sbjct: 81 FRASLVNEDGL 91
>UniRef100_UPI0000293917 UPI0000293917 UniRef100 entry
Length = 234
Score = 41.2 bits (95), Expect = 0.027
Identities = 25/67 (37%), Positives = 37/67 (54%), Gaps = 1/67 (1%)
Query: 164 LLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERVYG 223
LL LA H S K ++ S + RL FLG VL L++A+ ++R+P E G + R
Sbjct: 25 LLELALTHKSVLDPKGSYIES-NERLEFLGDRVLGLSIADMLIKRFPEENEGQIARRFSY 83
Query: 224 LIGKENL 230
L+ +E L
Sbjct: 84 LVSQEML 90
>UniRef100_UPI00002694E9 UPI00002694E9 UniRef100 entry
Length = 241
Score = 41.2 bits (95), Expect = 0.027
Identities = 22/71 (30%), Positives = 38/71 (52%)
Query: 160 QFDTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRE 219
Q + LL+LA H S K K + + +L FLG VL++A++E + R+P G + +
Sbjct: 21 QNEELLHLALTHKSFTEFKSKSGQRNNEKLEFLGDAVLDMAISELLMDRFPELDEGGLSK 80
Query: 220 RVYGLIGKENL 230
L+ ++ L
Sbjct: 81 FRASLVNEDGL 91
>UniRef100_Q65JQ5 Rnc [Bacillus licheniformis]
Length = 249
Score = 41.2 bits (95), Expect = 0.027
Identities = 25/84 (29%), Positives = 39/84 (45%)
Query: 160 QFDTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRE 219
Q + LLY AF H S +K + RL FLG VLEL +++F +YP G + +
Sbjct: 32 QNEKLLYQAFTHSSYVNEHRKKPYEDNERLEFLGDAVLELTISQFLYAKYPAMSEGDLTK 91
Query: 220 RVYGLIGKENLPKWIKAASLQNLI 243
++ + +L S L+
Sbjct: 92 LRAAIVCEPSLVSLAHELSFGELV 115
>UniRef100_P51833 Ribonuclease III [Bacillus subtilis]
Length = 249
Score = 41.2 bits (95), Expect = 0.027
Identities = 25/84 (29%), Positives = 39/84 (45%)
Query: 160 QFDTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRE 219
Q + LLY AF H S +K + RL FLG VLEL ++ F +YP G + +
Sbjct: 32 QNEKLLYQAFTHSSYVNEHRKKPYEDNERLEFLGDAVLELTISRFLFAKYPAMSEGDLTK 91
Query: 220 RVYGLIGKENLPKWIKAASLQNLI 243
++ + +L S +L+
Sbjct: 92 LRAAIVCEPSLVSLAHELSFGDLV 115
>UniRef100_Q9Z5U2 Ribonuclease III [Zymomonas mobilis]
Length = 222
Score = 40.8 bits (94), Expect = 0.035
Identities = 27/93 (29%), Positives = 41/93 (44%), Gaps = 16/93 (17%)
Query: 152 LEDDNPAKQFDTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPR 211
+E P+ + L A HPS H S + RL FLG VL L +A + +PR
Sbjct: 10 IEKFRPSAKDPALFLRAMTHPS-------HGNSDYQRLEFLGDRVLGLVIANWLYDLFPR 62
Query: 212 ELPGPMRERVYGLIGKEN---------LPKWIK 235
E G + R+ L+ + LP+W++
Sbjct: 63 EPEGKLSRRLNSLVSGASCASIARIVGLPQWLR 95
>UniRef100_Q5XDA6 Ribonuclease III [Streptococcus pyogenes]
Length = 230
Score = 40.4 bits (93), Expect = 0.046
Identities = 25/81 (30%), Positives = 41/81 (49%)
Query: 163 TLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERVY 222
TLL AF H S + S + RL FLG VL+L ++E+ +YP++ G M +
Sbjct: 20 TLLETAFTHTSYANEHRLLNVSHNERLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRS 79
Query: 223 GLIGKENLPKWIKAASLQNLI 243
++ +E+L + + S I
Sbjct: 80 MIVREESLAGFSRFCSFDAYI 100
Database: uniref100
Posted date: Jan 5, 2005 1:24 AM
Number of letters in database: 848,049,833
Number of sequences in database: 2,790,947
Lambda K H
0.318 0.137 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 462,614,776
Number of Sequences: 2790947
Number of extensions: 19600622
Number of successful extensions: 49857
Number of sequences better than 10.0: 169
Number of HSP's better than 10.0 without gapping: 83
Number of HSP's successfully gapped in prelim test: 86
Number of HSP's that attempted gapping in prelim test: 49720
Number of HSP's gapped (non-prelim): 192
length of query: 262
length of database: 848,049,833
effective HSP length: 125
effective length of query: 137
effective length of database: 499,181,458
effective search space: 68387859746
effective search space used: 68387859746
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 73 (32.7 bits)
Medicago: description of AC146561.3