BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144481.8 + phase: 0 /pseudo
(119 letters)
Database: uniref100
2,790,947 sequences; 848,049,833 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef100_Q9LW56 Similarity to long chain fatty alcohol oxidase ... 135 2e-31
UniRef100_Q8LGV0 Alcohol oxidase [Arabidopsis thaliana] 135 2e-31
UniRef100_Q9ZWB9 F21M11.7 protein [Arabidopsis thaliana] 135 2e-31
UniRef100_Q8LDP0 Hypothetical protein [Arabidopsis thaliana] 132 1e-30
UniRef100_Q9M0H4 Hypothetical protein AT4g28570 [Arabidopsis tha... 131 3e-30
UniRef100_Q94BP3 Hypothetical protein At4g28570 [Arabidopsis tha... 131 3e-30
UniRef100_Q6K9N5 Putative alcohol oxidase [Oryza sativa] 125 2e-28
UniRef100_Q8W5P5 Putative alcohol oxidase [Oryza sativa] 112 3e-24
UniRef100_Q8W5P8 Putative oxidase [Oryza sativa] 108 3e-23
UniRef100_Q8W5N7 Putative alcohol oxidase [Oryza sativa] 107 8e-23
UniRef100_Q8W5P3 Putative alcohol oxidase [Oryza sativa] 100 6e-21
UniRef100_O65709 Hypothetical protein AT4g19380 [Arabidopsis tha... 81 6e-15
UniRef100_Q5Z168 Putative oxidoreductase [Nocardia farcinica] 60 9e-09
UniRef100_Q72WA0 GMC oxidoreductase [Leptospira interrogans] 59 2e-08
UniRef100_Q7S2Z2 Hypothetical protein [Neurospora crassa] 57 1e-07
UniRef100_Q7NV56 Probable glucose-methanol-choline oxidoreductas... 53 1e-06
UniRef100_UPI00002A1D5A UPI00002A1D5A UniRef100 entry 52 2e-06
UniRef100_UPI0000321EAB UPI0000321EAB UniRef100 entry 52 4e-06
UniRef100_UPI00002D8D0E UPI00002D8D0E UniRef100 entry 51 7e-06
UniRef100_UPI000023F2E2 UPI000023F2E2 UniRef100 entry 50 9e-06
>UniRef100_Q9LW56 Similarity to long chain fatty alcohol oxidase [Arabidopsis
thaliana]
Length = 746
Score = 135 bits (341), Expect = 2e-31
Identities = 67/107 (62%), Positives = 84/107 (77%), Gaps = 1/107 (0%)
Query: 6 NGENQAWEAIGYHVDNDEST-SNIQKERPLEKGIVEAMNEDDLSLAQSLSNKGLEIDIDA 64
NGEN AWEAIGY V+ DE+ S ERPLEKGIVE M E + +L +SL++KGLE +D
Sbjct: 165 NGENPAWEAIGYRVNPDENKPSETHNERPLEKGIVETMEETEQTLLESLAHKGLEAVLDT 224
Query: 65 NSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRD 111
+ +++KCDVV+VGSG GGGVAA+VLA SGLKV+VLEKG+YFTP +
Sbjct: 225 EHDAIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSE 271
>UniRef100_Q8LGV0 Alcohol oxidase [Arabidopsis thaliana]
Length = 678
Score = 135 bits (341), Expect = 2e-31
Identities = 67/107 (62%), Positives = 84/107 (77%), Gaps = 1/107 (0%)
Query: 6 NGENQAWEAIGYHVDNDEST-SNIQKERPLEKGIVEAMNEDDLSLAQSLSNKGLEIDIDA 64
NGEN AWEAIGY V+ DE+ S ERPLEKGIVE M E + +L +SL++KGLE +D
Sbjct: 97 NGENPAWEAIGYRVNPDENKPSETHNERPLEKGIVETMEETEQTLLESLAHKGLEAVLDT 156
Query: 65 NSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRD 111
+ +++KCDVV+VGSG GGGVAA+VLA SGLKV+VLEKG+YFTP +
Sbjct: 157 EHDAIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSE 203
>UniRef100_Q9ZWB9 F21M11.7 protein [Arabidopsis thaliana]
Length = 736
Score = 135 bits (340), Expect = 2e-31
Identities = 60/111 (54%), Positives = 84/111 (75%)
Query: 4 DENGENQAWEAIGYHVDNDESTSNIQKERPLEKGIVEAMNEDDLSLAQSLSNKGLEIDID 63
+EN EN W+AI Y V+ E+ QKERPL++GI+E ED++++ Q + NKGL++ D
Sbjct: 155 NENSENPVWDAINYSVEIGENEDMEQKERPLDEGIIETAKEDEMTIKQRMINKGLKVTED 214
Query: 64 ANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSS 114
+ K++CD V+VGSGCGGGVAAA+LA SGL+V+V+EKGNYF PRD+S+
Sbjct: 215 RERDTYKIECDAVVVGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSA 265
>UniRef100_Q8LDP0 Hypothetical protein [Arabidopsis thaliana]
Length = 748
Score = 132 bits (333), Expect = 1e-30
Identities = 67/117 (57%), Positives = 85/117 (72%), Gaps = 5/117 (4%)
Query: 2 QYDENGENQAWEAIGYHVDNDESTSNI-----QKERPLEKGIVEAMNEDDLSLAQSLSNK 56
Q DEN +N A EAIGY +D E +SN +K RPLEKGI+E M+E DL++ QSL+ K
Sbjct: 162 QTDENLKNPALEAIGYCIDGTERSSNKKSEADEKRRPLEKGIIETMHESDLTITQSLTEK 221
Query: 57 GLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFS 113
G+ + D N+ +++CD V+VGSG GGGVAAA LA +GLKVLVLEKGNYFT D+S
Sbjct: 222 GVHVARDDGDNVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYS 278
>UniRef100_Q9M0H4 Hypothetical protein AT4g28570 [Arabidopsis thaliana]
Length = 748
Score = 131 bits (330), Expect = 3e-30
Identities = 66/117 (56%), Positives = 85/117 (72%), Gaps = 5/117 (4%)
Query: 2 QYDENGENQAWEAIGYHVDNDESTSNI-----QKERPLEKGIVEAMNEDDLSLAQSLSNK 56
Q DEN +N A EAIGY +D E +SN +K RPLEKGI+E M+E D+++ QSL+ K
Sbjct: 162 QTDENLKNPALEAIGYCIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEK 221
Query: 57 GLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFS 113
G+ + D N+ +++CD V+VGSG GGGVAAA LA +GLKVLVLEKGNYFT D+S
Sbjct: 222 GVHVARDDGDNVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYS 278
>UniRef100_Q94BP3 Hypothetical protein At4g28570 [Arabidopsis thaliana]
Length = 748
Score = 131 bits (330), Expect = 3e-30
Identities = 66/117 (56%), Positives = 85/117 (72%), Gaps = 5/117 (4%)
Query: 2 QYDENGENQAWEAIGYHVDNDESTSNI-----QKERPLEKGIVEAMNEDDLSLAQSLSNK 56
Q DEN +N A EAIGY +D E +SN +K RPLEKGI+E M+E D+++ QSL+ K
Sbjct: 162 QTDENLKNPALEAIGYCIDGTERSSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEK 221
Query: 57 GLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFS 113
G+ + D N+ +++CD V+VGSG GGGVAAA LA +GLKVLVLEKGNYFT D+S
Sbjct: 222 GVHVARDDGDNVYRIRCDAVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYS 278
>UniRef100_Q6K9N5 Putative alcohol oxidase [Oryza sativa]
Length = 770
Score = 125 bits (315), Expect = 2e-28
Identities = 64/114 (56%), Positives = 79/114 (69%), Gaps = 3/114 (2%)
Query: 4 DENGENQAWEAIGYHVDNDESTSNIQKE---RPLEKGIVEAMNEDDLSLAQSLSNKGLEI 60
DE+ EN W AIGY DE + E RPL+ G+VE +N D SL SL+ KGL +
Sbjct: 191 DESSENPHWRAIGYSPPTDEPPAEEHTEATKRPLDDGVVETINLTDASLPSSLAEKGLAV 250
Query: 61 DIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSS 114
DA N+ +V+CDV IVGSGCGGGVAAAVLA +G KV+V+EKGNYFT RD++S
Sbjct: 251 TDDAARNVCRVECDVAIVGSGCGGGVAAAVLAGAGHKVVVIEKGNYFTSRDYTS 304
>UniRef100_Q8W5P5 Putative alcohol oxidase [Oryza sativa]
Length = 590
Score = 112 bits (279), Expect = 3e-24
Identities = 62/124 (50%), Positives = 80/124 (64%), Gaps = 11/124 (8%)
Query: 1 MQYDENGENQAWEAIGYHVDNDE----------STSNIQKERPLEKGIVEAMNEDDLSLA 50
MQ +EN EN W+AIGY V+ S S + + RPL+ G+VE D +L
Sbjct: 1 MQVNENSENPYWKAIGYRVEEPRRDRAESMPSPSPSPVSR-RPLDNGVVETRALTDTTLL 59
Query: 51 QSLSNKGLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPR 110
+SL+ KGL + A+ V+CD VIVGSGCGGGVAAAVLAS+G KV+V+EKG+YFT
Sbjct: 60 RSLAAKGLAVRPGASDEHHTVRCDAVIVGSGCGGGVAAAVLASAGYKVVVVEKGDYFTKE 119
Query: 111 DFSS 114
D+SS
Sbjct: 120 DYSS 123
>UniRef100_Q8W5P8 Putative oxidase [Oryza sativa]
Length = 595
Score = 108 bits (270), Expect = 3e-23
Identities = 60/121 (49%), Positives = 78/121 (63%), Gaps = 11/121 (9%)
Query: 4 DENGENQAWEAIGYHVDNDE----------STSNIQKERPLEKGIVEAMNEDDLSLAQSL 53
+EN EN W+AIGY V+ S S + + RPL+ G+VE D +L +SL
Sbjct: 9 NENSENPYWKAIGYRVEEPRRDRAESMPSPSPSPVSR-RPLDNGVVETRALTDTTLLRSL 67
Query: 54 SNKGLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFS 113
+ KGL + A+ V+CD VIVGSGCGGGVAAAVLAS+G KV+V+EKG+YFT D+S
Sbjct: 68 AAKGLAVRPGASDEHHTVRCDAVIVGSGCGGGVAAAVLASAGYKVVVVEKGDYFTKEDYS 127
Query: 114 S 114
S
Sbjct: 128 S 128
>UniRef100_Q8W5N7 Putative alcohol oxidase [Oryza sativa]
Length = 756
Score = 107 bits (266), Expect = 8e-23
Identities = 65/134 (48%), Positives = 79/134 (58%), Gaps = 22/134 (16%)
Query: 1 MQYDENGENQAWEAIGYHVDNDESTSNIQKE-----------------RPLEKGIVEAMN 43
M DEN EN +W+AIGY V E QKE RPL+ G+VE
Sbjct: 143 MPVDENLENPSWKAIGYSVPAAEEEP--QKEEPTEATPTPAPATAPPCRPLDNGVVETKQ 200
Query: 44 EDDLSLAQSLSNKGLEIDIDANSNILK---VKCDVVIVGSGCGGGVAAAVLASSGLKVLV 100
DD +L SL+ KGL + A+S V CD V+VGSGCGGGVAAAVLAS+G KV+V
Sbjct: 201 LDDNALLMSLAEKGLALKTGASSPSAHHHTVLCDAVVVGSGCGGGVAAAVLASAGYKVVV 260
Query: 101 LEKGNYFTPRDFSS 114
+EKG+YF RD+SS
Sbjct: 261 VEKGDYFAARDYSS 274
>UniRef100_Q8W5P3 Putative alcohol oxidase [Oryza sativa]
Length = 637
Score = 100 bits (250), Expect = 6e-21
Identities = 55/117 (47%), Positives = 73/117 (62%), Gaps = 7/117 (5%)
Query: 4 DENGENQAWEAIGYHVDNDESTSNIQKE------RPLEKGIVEAMNEDDLSLAQSLSNKG 57
DE N +W+AIGY + + Q+ RPLE G+VE DD +L +SL KG
Sbjct: 61 DEKSRNPSWKAIGYAAPAAAAAAVEQRRPASPSRRPLEDGVVETRRMDDNALLRSLVEKG 120
Query: 58 LEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSS 114
L + ++ V+CD V+VGSGCGGGVAAAVLAS G KV+V+EKG+YF D++S
Sbjct: 121 LAVKTGTAAHHT-VQCDAVVVGSGCGGGVAAAVLASKGYKVVVVEKGDYFATEDYTS 176
>UniRef100_O65709 Hypothetical protein AT4g19380 [Arabidopsis thaliana]
Length = 678
Score = 80.9 bits (198), Expect = 6e-15
Identities = 48/125 (38%), Positives = 73/125 (58%), Gaps = 22/125 (17%)
Query: 4 DENGENQAWEAIGY------HVDNDESTSNIQKER-------PLEKGIVEAMNEDDLSLA 50
DE G N AW+AIGY H D++ + +K++ PL GIV+ + + ++
Sbjct: 87 DEKGRNLAWKAIGYNGPSPDHSDHEVELNEEKKKKKPEEIFGPLYNGIVDLKSPRE-AVE 145
Query: 51 QSLSNKGLEIDIDA-NSN-------ILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLE 102
+ L+ +G + N+N ++K++CD V+VGSG GGGVAA VLA +G KVLV+E
Sbjct: 146 KKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVGSGSGGGVAAGVLAKAGYKVLVIE 205
Query: 103 KGNYF 107
GNY+
Sbjct: 206 SGNYY 210
>UniRef100_Q5Z168 Putative oxidoreductase [Nocardia farcinica]
Length = 664
Score = 60.5 bits (145), Expect = 9e-09
Identities = 27/42 (64%), Positives = 34/42 (80%)
Query: 73 CDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSS 114
CDVV+VGSG GGG AAAVLA +GL V+VLE+G+Y+ DF +
Sbjct: 174 CDVVVVGSGAGGGTAAAVLAEAGLDVVVLERGDYYDDADFGN 215
>UniRef100_Q72WA0 GMC oxidoreductase [Leptospira interrogans]
Length = 537
Score = 59.3 bits (142), Expect = 2e-08
Identities = 25/53 (47%), Positives = 38/53 (71%)
Query: 66 SNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSSRRFL 118
+ I ++ DVV++GSG GG VAAA LA +G V+++E+G+YFTP F+ F+
Sbjct: 28 NGIWELNSDVVVIGSGAGGAVAAATLAKNGWNVILIEEGSYFTPAQFNGNEFI 80
>UniRef100_Q7S2Z2 Hypothetical protein [Neurospora crassa]
Length = 763
Score = 56.6 bits (135), Expect = 1e-07
Identities = 26/39 (66%), Positives = 31/39 (78%)
Query: 71 VKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTP 109
V DVVI+GSGCGGGV A VLA +G +VLV++KG YF P
Sbjct: 220 VDTDVVIIGSGCGGGVCAKVLAEAGHRVLVVDKGYYFNP 258
>UniRef100_Q7NV56 Probable glucose-methanol-choline oxidoreductase [Chromobacterium
violaceum]
Length = 516
Score = 53.1 bits (126), Expect = 1e-06
Identities = 25/44 (56%), Positives = 33/44 (74%)
Query: 69 LKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDF 112
L + DVVIVGSG GGG+AA VL+ +GL VL+LE+G ++ DF
Sbjct: 10 LSLAADVVIVGSGAGGGIAAEVLSRAGLDVLLLEEGGLYSSSDF 53
>UniRef100_UPI00002A1D5A UPI00002A1D5A UniRef100 entry
Length = 102
Score = 52.4 bits (124), Expect = 2e-06
Identities = 25/43 (58%), Positives = 31/43 (71%)
Query: 71 VKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFS 113
VK DVVIVG G GGVAA L ++GLKV+ +E G + PRDF+
Sbjct: 6 VKTDVVIVGMGAAGGVAALPLTNAGLKVVGIEAGGWLNPRDFA 48
>UniRef100_UPI0000321EAB UPI0000321EAB UniRef100 entry
Length = 263
Score = 51.6 bits (122), Expect = 4e-06
Identities = 23/44 (52%), Positives = 32/44 (72%), Gaps = 2/44 (4%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPR--DFSSR 115
DVV++GSGCGG AA +LA +G +V+++E+G YFT DF R
Sbjct: 6 DVVVIGSGCGGATAAKLLAEAGKRVIIVEQGGYFTAARGDFDQR 49
>UniRef100_UPI00002D8D0E UPI00002D8D0E UniRef100 entry
Length = 102
Score = 50.8 bits (120), Expect = 7e-06
Identities = 24/42 (57%), Positives = 30/42 (71%)
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFS 113
K DVVIVG G GGVAA L ++GLKV+ +E G + PRDF+
Sbjct: 7 KTDVVIVGMGAAGGVAALPLTNAGLKVVGIEAGGWLNPRDFA 48
>UniRef100_UPI000023F2E2 UPI000023F2E2 UniRef100 entry
Length = 925
Score = 50.4 bits (119), Expect = 9e-06
Identities = 25/48 (52%), Positives = 34/48 (70%)
Query: 60 IDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYF 107
I I+A ++ DVVIVGSGCGGGV+A LA +G KVLV++K ++
Sbjct: 206 IQIEAGDEPHVIETDVVIVGSGCGGGVSAKNLAEAGHKVLVVDKAYHY 253
Database: uniref100
Posted date: Jan 5, 2005 1:24 AM
Number of letters in database: 848,049,833
Number of sequences in database: 2,790,947
Lambda K H
0.313 0.134 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 206,929,592
Number of Sequences: 2790947
Number of extensions: 7987845
Number of successful extensions: 35878
Number of sequences better than 10.0: 2219
Number of HSP's better than 10.0 without gapping: 2051
Number of HSP's successfully gapped in prelim test: 168
Number of HSP's that attempted gapping in prelim test: 33677
Number of HSP's gapped (non-prelim): 2335
length of query: 119
length of database: 848,049,833
effective HSP length: 95
effective length of query: 24
effective length of database: 582,909,868
effective search space: 13989836832
effective search space used: 13989836832
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 67 (30.4 bits)
Medicago: description of AC144481.8