BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124966.6 + phase: 0
(176 letters)
Database: uniref100
2,790,947 sequences; 848,049,833 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef100_O82396 Putative bHLH transcription factor [Arabidopsis... 60 2e-08
UniRef100_Q8S3F3 Putative bHLH transcription factor [Arabidopsis... 60 2e-08
UniRef100_O81348 Symbiotic ammonium transporter [Glycine max] 59 4e-08
UniRef100_Q8S3F0 Putative bHLH transcription factor [Arabidopsis... 56 5e-07
UniRef100_Q9T072 Hypothetical protein AT4g37850 [Arabidopsis tha... 56 5e-07
UniRef100_Q75GI1 Putative symbiotic ammonium transport protein [... 50 3e-05
UniRef100_O82397 Putative bHLH transcription factor [Arabidopsis... 49 6e-05
UniRef100_Q8S3F2 Putative bHLH transcription factor [Arabidopsis... 49 6e-05
UniRef100_Q67Z70 Putative bHLH transcription factor [Arabidopsis... 48 1e-04
UniRef100_O82398 Putative bHLH transcription factor [Arabidopsis... 48 1e-04
UniRef100_Q8S3F1 Putative bHLH transcription factor [Arabidopsis... 48 1e-04
UniRef100_Q9ZSQ9 Transporter homolog [Mesembryanthemum crystalli... 48 1e-04
UniRef100_Q8LSP3 Putative bHLH transcription factor [Oryza sativa] 47 3e-04
UniRef100_Q84R60 Putative ammonium transporter [Oryza sativa] 46 5e-04
UniRef100_Q8IE49 DNA-directed RNA polymerase 3 largest subunit [... 43 0.003
UniRef100_P27625 DNA-directed RNA polymerase III largest subunit... 43 0.003
UniRef100_Q657A4 Regulatory protein B-Peru-like [Oryza sativa] 42 0.009
UniRef100_Q84R79 Putative ammonium transporter [Oryza sativa] 41 0.015
UniRef100_Q39204 Transcription factor AtMYC2 [Arabidopsis thaliana] 41 0.015
UniRef100_Q8QQT9 Envelope glycoprotein [Porcine reproductive and... 40 0.020
>UniRef100_O82396 Putative bHLH transcription factor [Arabidopsis thaliana]
Length = 288
Score = 60.5 bits (145), Expect = 2e-08
Identities = 36/91 (39%), Positives = 51/91 (55%)
Query: 73 PSQYILSFENSTMQPSPNSDIATCSSIMVQETTTLNNNVSSELPKIIKKRTKNLRSSCEM 132
PS ILSFE + + ++ S +E + + + + K+ RS
Sbjct: 65 PSSRILSFEKTGLHVMNHNSPNLIFSPKDEEIGLPEHKKAELIIRGTKRAQSLTRSQSNA 124
Query: 133 QDHIMAERKRRQVLTERFIALSATIPGLKKV 163
QDHI+AERKRR+ LT+RF+ALSA IPGLKK+
Sbjct: 125 QDHILAERKRREKLTQRFVALSALIPGLKKM 155
>UniRef100_Q8S3F3 Putative bHLH transcription factor [Arabidopsis thaliana]
Length = 304
Score = 60.5 bits (145), Expect = 2e-08
Identities = 36/91 (39%), Positives = 51/91 (55%)
Query: 73 PSQYILSFENSTMQPSPNSDIATCSSIMVQETTTLNNNVSSELPKIIKKRTKNLRSSCEM 132
PS ILSFE + + ++ S +E + + + + K+ RS
Sbjct: 65 PSSRILSFEKTGLHVMNHNSPNLIFSPKDEEIGLPEHKKAELIIRGTKRAQSLTRSQSNA 124
Query: 133 QDHIMAERKRRQVLTERFIALSATIPGLKKV 163
QDHI+AERKRR+ LT+RF+ALSA IPGLKK+
Sbjct: 125 QDHILAERKRREKLTQRFVALSALIPGLKKM 155
>UniRef100_O81348 Symbiotic ammonium transporter [Glycine max]
Length = 347
Score = 59.3 bits (142), Expect = 4e-08
Identities = 55/174 (31%), Positives = 83/174 (47%), Gaps = 36/174 (20%)
Query: 5 KSHFYLDEDKFQREIVQHQPSFSFESGNSQSSTIQYMNNIVDNVCVTNTVHENIAQEYTW 64
+ H + D F P S E+ SQ+ +Y + DN + + N +Q+ T
Sbjct: 49 QKHSFSDNSNFN-------PKTSMET--SQTGIERYAKQLGDN-----SWNHNKSQQQTP 94
Query: 65 ATNSQQLPPSQYILSFENST-------MQPS-----PNSDIATCSSIMVQETTTLNNNV- 111
T Q +LSF N+ ++P P D + +++ + T N N
Sbjct: 95 ET---QFASCSNLLSFVNTNYTSELGLVKPKVEMACPKIDNNALADMLISQGTLGNQNYI 151
Query: 112 --SSELPKIIKKRTKNLRSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
+S+ K IK R K + QDHI+AERKRR+ L++RFIALSA +PGLKK+
Sbjct: 152 FKASQETKKIKTRPK----LSQPQDHIIAERKRREKLSQRFIALSALVPGLKKM 201
>UniRef100_Q8S3F0 Putative bHLH transcription factor [Arabidopsis thaliana]
Length = 304
Score = 55.8 bits (133), Expect = 5e-07
Identities = 40/118 (33%), Positives = 56/118 (46%), Gaps = 15/118 (12%)
Query: 61 EYTWATNSQQLPP-----------SQYILSFENSTMQPSPNSDIATCSSIMVQETTTLNN 109
E T+ + S LPP S ILSFE+ + T + +
Sbjct: 40 ETTYISPSSHLPPNSKPHHIHRHSSSRILSFEDYGSNDMEHEYSPTYLNSIFSPKLEAQV 99
Query: 110 NVSSELPKIIKKRTKNL----RSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
+ + +K TK R+ QDHI+AERKRR+ LT+RF+ALSA +PGLKK+
Sbjct: 100 QPHQKSDEFNRKGTKRAQPFSRNQSNAQDHIIAERKRREKLTQRFVALSALVPGLKKM 157
>UniRef100_Q9T072 Hypothetical protein AT4g37850 [Arabidopsis thaliana]
Length = 314
Score = 55.8 bits (133), Expect = 5e-07
Identities = 40/118 (33%), Positives = 56/118 (46%), Gaps = 15/118 (12%)
Query: 61 EYTWATNSQQLPP-----------SQYILSFENSTMQPSPNSDIATCSSIMVQETTTLNN 109
E T+ + S LPP S ILSFE+ + T + +
Sbjct: 40 ETTYISPSSHLPPNSKPHHIHRHSSSRILSFEDYGSNDMEHEYSPTYLNSIFSPKLEAQV 99
Query: 110 NVSSELPKIIKKRTKNL----RSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
+ + +K TK R+ QDHI+AERKRR+ LT+RF+ALSA +PGLKK+
Sbjct: 100 QPHQKSDEFNRKGTKRAQPFSRNQSNAQDHIIAERKRREKLTQRFVALSALVPGLKKM 157
>UniRef100_Q75GI1 Putative symbiotic ammonium transport protein [Oryza sativa]
Length = 359
Score = 49.7 bits (117), Expect = 3e-05
Identities = 22/37 (59%), Positives = 31/37 (83%)
Query: 127 RSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
R + + Q+HI+AERKRR+ L++RFIALS +PGLKK+
Sbjct: 174 RPASQNQEHILAERKRREKLSQRFIALSKIVPGLKKM 210
>UniRef100_O82397 Putative bHLH transcription factor [Arabidopsis thaliana]
Length = 284
Score = 48.9 bits (115), Expect = 6e-05
Identities = 25/54 (46%), Positives = 37/54 (68%), Gaps = 6/54 (11%)
Query: 115 LPKIIKKRTKN------LRSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKK 162
+ K++ + TK RS ++H++AERKRR+ L+E+FIALSA +PGLKK
Sbjct: 94 MDKLVGRGTKRKTCSHGTRSPVLAKEHVLAERKRREKLSEKFIALSALLPGLKK 147
>UniRef100_Q8S3F2 Putative bHLH transcription factor [Arabidopsis thaliana]
Length = 295
Score = 48.9 bits (115), Expect = 6e-05
Identities = 25/54 (46%), Positives = 37/54 (68%), Gaps = 6/54 (11%)
Query: 115 LPKIIKKRTKN------LRSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKK 162
+ K++ + TK RS ++H++AERKRR+ L+E+FIALSA +PGLKK
Sbjct: 94 MDKLVGRGTKRKTCSHGTRSPVLAKEHVLAERKRREKLSEKFIALSALLPGLKK 147
>UniRef100_Q67Z70 Putative bHLH transcription factor [Arabidopsis thaliana]
Length = 320
Score = 47.8 bits (112), Expect = 1e-04
Identities = 21/31 (67%), Positives = 27/31 (86%)
Query: 132 MQDHIMAERKRRQVLTERFIALSATIPGLKK 162
+++H++AERKRRQ L ER IALSA +PGLKK
Sbjct: 130 LKEHVLAERKRRQKLNERLIALSALLPGLKK 160
>UniRef100_O82398 Putative bHLH transcription factor [Arabidopsis thaliana]
Length = 322
Score = 47.8 bits (112), Expect = 1e-04
Identities = 21/31 (67%), Positives = 27/31 (86%)
Query: 132 MQDHIMAERKRRQVLTERFIALSATIPGLKK 162
+++H++AERKRRQ L ER IALSA +PGLKK
Sbjct: 130 LKEHVLAERKRRQKLNERLIALSALLPGLKK 160
>UniRef100_Q8S3F1 Putative bHLH transcription factor [Arabidopsis thaliana]
Length = 320
Score = 47.8 bits (112), Expect = 1e-04
Identities = 21/31 (67%), Positives = 27/31 (86%)
Query: 132 MQDHIMAERKRRQVLTERFIALSATIPGLKK 162
+++H++AERKRRQ L ER IALSA +PGLKK
Sbjct: 130 LKEHVLAERKRRQKLNERLIALSALLPGLKK 160
>UniRef100_Q9ZSQ9 Transporter homolog [Mesembryanthemum crystallinum]
Length = 300
Score = 47.8 bits (112), Expect = 1e-04
Identities = 20/31 (64%), Positives = 28/31 (89%)
Query: 133 QDHIMAERKRRQVLTERFIALSATIPGLKKV 163
Q+H++AERKRR+ +T+RF ALSA +PGLKK+
Sbjct: 117 QEHVLAERKRREKMTQRFHALSALVPGLKKM 147
>UniRef100_Q8LSP3 Putative bHLH transcription factor [Oryza sativa]
Length = 451
Score = 46.6 bits (109), Expect = 3e-04
Identities = 33/103 (32%), Positives = 51/103 (49%), Gaps = 8/103 (7%)
Query: 81 ENSTMQPSPNSDIATCSSIMVQ-ETTTLNNN-----VSSELPKIIKKRTKNLRSSCEMQD 134
+ QPSP+S + VQ TTTL+ + + + + R Q+
Sbjct: 228 KEQVQQPSPSSSNVLSFAGQVQGSTTTLDFSGRGWQQDDGVGVFQQPPERRSRPPANAQE 287
Query: 135 HIMAERKRRQVLTERFIALSATIPGLKKVS--FLYKPTISLIK 175
H++AERKRR+ L ++F+AL+ +PGLKK L TI +K
Sbjct: 288 HVIAERKRREKLQQQFVALATIVPGLKKTDKISLLGSTIDYVK 330
>UniRef100_Q84R60 Putative ammonium transporter [Oryza sativa]
Length = 353
Score = 45.8 bits (107), Expect = 5e-04
Identities = 20/31 (64%), Positives = 26/31 (83%)
Query: 133 QDHIMAERKRRQVLTERFIALSATIPGLKKV 163
Q+HI+AERKRR+ + +RFI LS IPGLKK+
Sbjct: 191 QEHIIAERKRREKINQRFIELSTVIPGLKKM 221
>UniRef100_Q8IE49 DNA-directed RNA polymerase 3 largest subunit [Plasmodium falciparum]
Length = 2356
Score = 43.1 bits (100), Expect = 0.003
Identities = 33/144 (22%), Positives = 68/144 (46%), Gaps = 16/144 (11%)
Query: 5 KSHFYLDEDKFQREIVQHQPSFSFESGNSQSSTIQYMN---NIVDNVCVTNTVHENIAQE 61
K+ F++++ K + H+ + E N+Q IQY N N + + + NT H + +
Sbjct: 1347 KAKFFIEKKKGK----MHECNDDIEYNNTQYDNIQYNNISCNYIKSQNLENT-HHQVNND 1401
Query: 62 YTWATNSQQLPPSQY--ILSFENSTMQPSPNSDIATCSSIMVQETTTLNNNVSSELPKII 119
++ N+ LPP +Y I F N ++ ++M ++ LNN+ + +
Sbjct: 1402 LSFIKNNVILPPKEYHSIFHFVNDYR------NVVEIKNLMDKKKIFLNNSEKNVVQSKY 1455
Query: 120 KKRTKNLRSSCEMQDHIMAERKRR 143
+ +KNL+ E+ ++I K++
Sbjct: 1456 NRMSKNLKKKIEIINNIYRNEKKK 1479
>UniRef100_P27625 DNA-directed RNA polymerase III largest subunit [Plasmodium
falciparum]
Length = 2339
Score = 43.1 bits (100), Expect = 0.003
Identities = 33/144 (22%), Positives = 68/144 (46%), Gaps = 16/144 (11%)
Query: 5 KSHFYLDEDKFQREIVQHQPSFSFESGNSQSSTIQYMN---NIVDNVCVTNTVHENIAQE 61
K+ F++++ K + H+ + E N+Q IQY N N + + + NT H + +
Sbjct: 1337 KAKFFIEKKKGK----MHECNDDIEYNNTQYDNIQYNNISCNYIKSQNLENT-HHQVNND 1391
Query: 62 YTWATNSQQLPPSQY--ILSFENSTMQPSPNSDIATCSSIMVQETTTLNNNVSSELPKII 119
++ N+ LPP +Y I F N ++ ++M ++ LNN+ + +
Sbjct: 1392 LSFIKNNVILPPKEYHSIFHFVNDYR------NVVEIKNLMDKKKIFLNNSEKNVVQSKY 1445
Query: 120 KKRTKNLRSSCEMQDHIMAERKRR 143
+ +KNL+ E+ ++I K++
Sbjct: 1446 NRMSKNLKKKIEIINNIYRNEKKK 1469
>UniRef100_Q657A4 Regulatory protein B-Peru-like [Oryza sativa]
Length = 370
Score = 41.6 bits (96), Expect = 0.009
Identities = 18/49 (36%), Positives = 33/49 (66%), Gaps = 2/49 (4%)
Query: 129 SCEMQDHIMAERKRRQVLTERFIALSATIPGLKKV--SFLYKPTISLIK 175
SC +++H+M+ER+RR+ L E F+ L + +P + KV + + TI+ +K
Sbjct: 183 SCSIKNHVMSERRRREKLNEMFLILKSLVPSIDKVDKASILSETIAYLK 231
>UniRef100_Q84R79 Putative ammonium transporter [Oryza sativa]
Length = 301
Score = 40.8 bits (94), Expect = 0.015
Identities = 17/30 (56%), Positives = 25/30 (82%)
Query: 134 DHIMAERKRRQVLTERFIALSATIPGLKKV 163
+H++AERKRR+ + +RF+ LSA IP LKK+
Sbjct: 130 EHVVAERKRREKINQRFMELSAVIPKLKKM 159
>UniRef100_Q39204 Transcription factor AtMYC2 [Arabidopsis thaliana]
Length = 623
Score = 40.8 bits (94), Expect = 0.015
Identities = 27/87 (31%), Positives = 44/87 (50%), Gaps = 11/87 (12%)
Query: 77 ILSFENSTMQPSPNSDIATCSSIMVQETTTLNNNVSSELPKIIKKRTKNLRSSCEMQDHI 136
+LSF + T S +SD+ E + + + PK ++ N R E +H+
Sbjct: 406 VLSFGDKTAGESDHSDL---------EASVVKEVAVEKRPKKRGRKPANGRE--EPLNHV 454
Query: 137 MAERKRRQVLTERFIALSATIPGLKKV 163
AER+RR+ L +RF AL A +P + K+
Sbjct: 455 EAERQRREKLNQRFYALRAVVPNVSKM 481
>UniRef100_Q8QQT9 Envelope glycoprotein [Porcine reproductive and respiratory
syndrome virus]
Length = 201
Score = 40.4 bits (93), Expect = 0.020
Identities = 32/110 (29%), Positives = 53/110 (48%), Gaps = 23/110 (20%)
Query: 26 FSFESGNSQSSTIQYMNNIVDNVCVTNTVHENIAQEYTWATNSQQLPP----------SQ 75
+SF +GN SST QY+ N+ +C N E ++ + WA + L P SQ
Sbjct: 29 WSFVAGNDNSSTYQYIYNL--TICELNGT-EWLSGHFAWAAETFVLYPVVNQSFHWVSSQ 85
Query: 76 YILSFENSTMQPSPNSDI-------ATCSSIMVQETTTLNNNVSSELPKI 118
+ S + P P D+ ATC+++++ + + + +SS LPKI
Sbjct: 86 QAIFLMRSVLAPCPLQDLLTGGMCSATCTALVLSQHSCV---LSSALPKI 132
Database: uniref100
Posted date: Jan 5, 2005 1:24 AM
Number of letters in database: 848,049,833
Number of sequences in database: 2,790,947
Lambda K H
0.314 0.127 0.354
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 269,387,407
Number of Sequences: 2790947
Number of extensions: 9480308
Number of successful extensions: 34814
Number of sequences better than 10.0: 202
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 118
Number of HSP's that attempted gapping in prelim test: 34674
Number of HSP's gapped (non-prelim): 239
length of query: 176
length of database: 848,049,833
effective HSP length: 119
effective length of query: 57
effective length of database: 515,927,140
effective search space: 29407846980
effective search space used: 29407846980
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 70 (31.6 bits)
Medicago: description of AC124966.6