BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149576.7 + phase: 1 /pseudo
(576 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Y139_UREPA (Q9PR05) Hypothetical protein UU139 43 0.002
SYFB_CLOPE (Q8XJ76) Phenylalanyl-tRNA synthetase beta chain (EC ... 35 0.41
HIS4_YEAST (P40545) 1-(5-phosphoribosyl)-5-[(5-phosphoribosylami... 33 1.6
TRA5_LACLA (P35881) Transposase for insertion sequence element I... 33 2.0
YPT4_CAEEL (P41882) Hypothetical protein F37A4.4 in chromosome III 33 2.6
HIS4_CANGA (Q6FPN5) 1-(5-phosphoribosyl)-5-[(5-phosphoribosylami... 33 2.6
RBP2_PLAVB (Q00799) Reticulocyte binding protein 2 precursor (Pv... 32 3.5
GATB_AQUAE (O66766) Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransfe... 32 4.5
YHIJ_ECOLI (P37627) Hypothetical protein yhiJ 32 5.9
>Y139_UREPA (Q9PR05) Hypothetical protein UU139
Length = 236
Score = 43.1 bits (100), Expect = 0.002
Identities = 36/133 (27%), Positives = 63/133 (47%), Gaps = 8/133 (6%)
Query: 173 SITNIKRVYNECHKFKKAKRGDLTEMQYLISKLEENEYVNYVREKPESQIVQDIFWTHPT 232
SITN+ N+ H ++ + + + L + VNY+ EK ++ I FWT
Sbjct: 67 SITNLD--INKFHSYQIVNINENKNIYNERNSLILFDLVNYIIEKNQNSIRHINFWTIQM 124
Query: 233 SVK-----LFNTFPTVLIMDSTYKTNLYRMPLFEIVGVTSTYLTYSVGFA-FMMSKKEDN 286
SV + F ++D +K ++++ E GV ST L ++ GF F++ K N
Sbjct: 125 SVVNWIYFITYNFNNSYLIDPNWKKYVFKIKKDESKGVISTNLLHNYGFVDFVVQSKSQN 184
Query: 287 FTWALQMLLKLLK 299
F A ++ ++LK
Sbjct: 185 FLKAYRVQDEILK 197
>SYFB_CLOPE (Q8XJ76) Phenylalanyl-tRNA synthetase beta chain (EC
6.1.1.20) (Phenylalanine--tRNA ligase beta chain)
(PheRS)
Length = 793
Score = 35.4 bits (80), Expect = 0.41
Identities = 29/109 (26%), Positives = 51/109 (46%), Gaps = 7/109 (6%)
Query: 237 FNTFPTVLIMDSTYKTNLYRMPLFE---IVGVTSTYLTYSVGFAFMMSKKEDNFTW---- 289
+N P LI ST K + L + I + ++ L S+ ++F+ K D
Sbjct: 473 YNNIPATLISTSTEKEAKDKKQLLDEKVIDTMLASGLNQSISYSFVSPKVFDKICVPEDS 532
Query: 290 ALQMLLKLLKPNSDMPKVVVTDRDPSMMNAVANVLPDSSAILCYFHVGK 338
+L+ ++K+ P + V+ T PSMM ++A S+ I+ F +GK
Sbjct: 533 SLRNVVKIKNPLGEDYSVMRTSTLPSMMESLARNYSRSNEIVRLFEIGK 581
>HIS4_YEAST (P40545)
1-(5-phosphoribosyl)-5-[(5-
phosphoribosylamino)methylideneamino]
imidazole-4-carboxamide isomerase (EC 5.3.1.16)
(Phosphoribosylformimino-5-aminoimidazole carboxamide
ribotide isomerase) (5-proFAR isomerase)
Length = 261
Score = 33.5 bits (75), Expect = 1.6
Identities = 19/47 (40%), Positives = 28/47 (59%), Gaps = 9/47 (19%)
Query: 362 KIVDEESHSKLVDTIFDASEKLVESHTQELYAGNLVEFQDACKDHPK 408
K+VDE SH K VD F +S +++ GNLV+F+D C+ + K
Sbjct: 222 KLVDELSHGK-VDLTFGSS--------LDIFGGNLVKFEDCCRWNEK 259
>TRA5_LACLA (P35881) Transposase for insertion sequence element
IS905
Length = 391
Score = 33.1 bits (74), Expect = 2.0
Identities = 29/139 (20%), Positives = 60/139 (42%), Gaps = 11/139 (7%)
Query: 242 TVLIMDSTY----KTNLYRMPLFEIVGVTSTYLTYSVGFAFMMSKKEDNFTWALQMLLKL 297
+VL +D TY + + + + +G+T +G+ ++ E+N +W+ +L KL
Sbjct: 158 SVLFLDGTYLPLRRGTVSKECIHIALGITPEGQKAVLGYE--IAPNENNASWST-LLDKL 214
Query: 298 LKPNSDMPKVVVTDRDPSMMNAVANVLPDSSAILCYFHVGKNIRSRIITDCKVKQNVVVV 357
+VVTD + ++ P + C H+ +N+ S++ K V++
Sbjct: 215 QNQGIQQVSLVVTDGFKGLEQIISQAYPLAKQQRCLIHISRNLASKV----KRADRAVIL 270
Query: 358 DGQKKIVDEESHSKLVDTI 376
+ K I E+ V +
Sbjct: 271 EQFKTIYRAENLEMAVQAL 289
>YPT4_CAEEL (P41882) Hypothetical protein F37A4.4 in chromosome III
Length = 1163
Score = 32.7 bits (73), Expect = 2.6
Identities = 39/227 (17%), Positives = 85/227 (37%), Gaps = 12/227 (5%)
Query: 166 LKKKKKESITNIKRVYNECHKFKKAKRGDLTEMQYLISKLEENEYVNYVREKPESQIVQD 225
LK K+ + N+ Y +RG ++ LI + ++N + E I +
Sbjct: 841 LKAVKRGAYINVCNKYGNTALHVATRRGYQNLVEILIKHGADRSFLNPQNKTAEQMIPVN 900
Query: 226 IFWTHPTSVKLFNTFPTVLIMDSTYKTNLYRMPLFEIVGVTSTYLTYSVGFAFMMSKKED 285
TH ++ F + ++ + Y+ +++ + E V+S ++ ++ + +D
Sbjct: 901 YQETHKEKIERFKSIESIY---NKYRKKKFKLCVPEKFPVSSFHI-------YIEDRTDD 950
Query: 286 NFTWALQMLLKLLKPNSDMPKVVVTDRDPSMMNAVANVLPDSSAILCYFHVGKNIRSRII 345
N T + K SD + T D + ++ FH +R +
Sbjct: 951 NVT--NEFTTKFQSITSDEAMITTTHVVVKTTEDGILETDDLNLLIWIFHGSIIVRDTWM 1008
Query: 346 TDCKVKQNVVVVDGQKKIVDEESHSKLVDTIFDASEKLVESHTQELY 392
DC + ++ D + + + DT+ S + ++ T LY
Sbjct: 1009 VDCLRDEKLIEKDCDYLVEKVKYKGIIYDTVTQWSNAMAKATTPFLY 1055
>HIS4_CANGA (Q6FPN5)
1-(5-phosphoribosyl)-5-[(5-
phosphoribosylamino)methylideneamino]
imidazole-4-carboxamide isomerase (EC 5.3.1.16)
(Phosphoribosylformimino-5-aminoimidazole carboxamide
ribotide isomerase) (5-proFAR isomerase)
Length = 261
Score = 32.7 bits (73), Expect = 2.6
Identities = 19/62 (30%), Positives = 30/62 (47%), Gaps = 4/62 (6%)
Query: 347 DCKVKQNVVVVDGQKKIVDEESHSKLVDTIFDASEKLVESHTQELYAGNLVEFQDACKDH 406
D + +V G K I D E LVD + + + L + +++ G LV FQD C+ +
Sbjct: 202 DINKEVKIVYAGGAKSISDLE----LVDKLSNGTVDLTYGSSLDIFGGKLVRFQDCCEWN 257
Query: 407 PK 408
K
Sbjct: 258 SK 259
>RBP2_PLAVB (Q00799) Reticulocyte binding protein 2 precursor
(PvRBP-2)
Length = 2867
Score = 32.3 bits (72), Expect = 3.5
Identities = 63/319 (19%), Positives = 123/319 (37%), Gaps = 44/319 (13%)
Query: 156 FGETKK--YLTNLKKKKKESITNIKRVYNECHKFKKAKRGD--LTEMQYLISK----LEE 207
F E K ++ N+KK K +I +K++ E + +KR + + E+ ++I K L +
Sbjct: 332 FDEKKSISFMDNMKKIHKSAIDTLKKMKAELNTSLDSKRTEFIIGEIGHMIEKFNLHLTK 391
Query: 208 NEYVN-YVREKPESQIVQDIFWTHPTSVKLFNTFPTVLIMDSTYKTNLYRMPLFEIVGVT 266
Y + +++ P ++ DI+ V+L F + +K +L + +FE + +
Sbjct: 392 IRYASAFIKSIPLQKVESDIY-----RVELKTLFYVAAKHYADFKFSLEHLKMFENLSKS 446
Query: 267 STYLTYSVGFAFMMSKKEDNFTWALQMLLKLLKPNSDMPKVVVTDRDPSMMNAVANVLPD 326
+ YS K E + + L+ + SD+ ++ D + + +A + +
Sbjct: 447 KEKMLYST-----FEKLEGDLLNKINTLMGSEQSTSDLTS-IIADSEKIIKSAESLINSS 500
Query: 327 SSAILCYF--------HVGKNIRSRI--ITDCKVKQNVVVVDGQKKIVDEESHSKLVDTI 376
S I Y + KN I + + K N ++ + ES + ++T
Sbjct: 501 SEEIAKYALDSNEKINEIKKNYDQNILKVREFINKSNGLITSVKGTSQLSESDKQQIETK 560
Query: 377 FDASEKLVESHTQELYAGNLVEFQDACKDHPKFLEYVETTTLKPFKDKLVRAWMELVLHL 436
+ +K + + + + K K +T K F DKL E
Sbjct: 561 IEEIKKKKKDILER--GKEFINIMNEIKKKKKSNSSNSSTNSKEFTDKLKELETEF---- 614
Query: 437 GCRTTNRVEGAHGVVKEYL 455
EG + VK YL
Sbjct: 615 --------EGLNKTVKGYL 625
>GATB_AQUAE (O66766) Aspartyl/glutamyl-tRNA(Asn/Gln)
amidotransferase subunit B (EC 6.3.5.-) (Asp/Glu-ADT
subunit B)
Length = 478
Score = 32.0 bits (71), Expect = 4.5
Identities = 24/97 (24%), Positives = 40/97 (40%), Gaps = 5/97 (5%)
Query: 289 WALQMLLKLLKPNSDMPKVVVTDRDPSMMNAVANVLPDSSAILCYFHVGKNIRSRIITDC 348
W + LL LL+ K + + P +A ++ + +GK + ++
Sbjct: 346 WLINDLLGLLRD-----KGISIEESPVKPEHLAELVKLIKEKVISTKIGKEVIKEMVETG 400
Query: 349 KVKQNVVVVDGQKKIVDEESHSKLVDTIFDASEKLVE 385
K +V G K+I DE +LV IF+ K VE
Sbjct: 401 KTPSQIVEEKGLKQITDENQIKELVKKIFEKHPKEVE 437
>YHIJ_ECOLI (P37627) Hypothetical protein yhiJ
Length = 540
Score = 31.6 bits (70), Expect = 5.9
Identities = 19/82 (23%), Positives = 38/82 (46%)
Query: 447 AHGVVKEYLSTSKGDLGTCSQKIDEMLANQFGEIQSSFGRSVTVLEHRYKDVTLYSGLGG 506
A+G+ + S ++ LG +KID+ +F + S T+ + ++ SG+
Sbjct: 38 ANGIGDDISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRA 97
Query: 507 HMSRQAMNFIFVEEARSRKTLC 528
H+ R+ N+ F+ S+ C
Sbjct: 98 HILRRNNNYQFIVTINSKNYGC 119
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.334 0.144 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,736,335
Number of Sequences: 164201
Number of extensions: 2446478
Number of successful extensions: 10272
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 10265
Number of HSP's gapped (non-prelim): 15
length of query: 576
length of database: 59,974,054
effective HSP length: 116
effective length of query: 460
effective length of database: 40,926,738
effective search space: 18826299480
effective search space used: 18826299480
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 69 (31.2 bits)
Medicago: description of AC149576.7