BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149495.3 + phase: 0
(412 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Y939_SULTO (Q973G3) Hypothetical protein ST0939 precursor 33 1.3
Y511_CLOAB (Q97LP3) Hypothetical UPF0042 protein CAC0511 33 1.7
MSP1_PLAF3 (P19598) Merozoite surface protein 1 precursor (Meroz... 33 1.7
RPC1_PLAFA (P27625) DNA-directed RNA polymerase III largest subu... 32 2.3
MSP1_PLAFM (P08569) Merozoite surface protein 1 precursor (Meroz... 32 3.0
MSP1_PLAFF (P13819) Merozoite surface protein 1 precursor (Meroz... 32 3.0
SYA_BUCBP (P59420) Alanyl-tRNA synthetase (EC 6.1.1.7) (Alanine-... 32 3.9
R8L2_ARATH (Q9MAG6) Probable disease resistance RPP8-like protein 2 32 3.9
DPOQ_HUMAN (O75417) DNA polymerase theta (EC 2.7.7.7) (DNA polym... 32 3.9
MSP1_PLAFW (P04933) Merozoite surface protein 1 precursor (Meroz... 31 6.6
MSP1_PLAFK (P04932) Merozoite surface protein 1 precursor (Meroz... 31 6.6
KTHY_STRPY (Q9A190) Thymidylate kinase (EC 2.7.4.9) (dTMP kinase) 30 8.6
KTHY_STRP8 (Q8P2B1) Thymidylate kinase (EC 2.7.4.9) (dTMP kinase) 30 8.6
KTHY_STRP3 (Q8K8H4) Thymidylate kinase (EC 2.7.4.9) (dTMP kinase) 30 8.6
EPD2_SALSA (P28772) Ependymin II precursor (EPD-II) 30 8.6
>Y939_SULTO (Q973G3) Hypothetical protein ST0939 precursor
Length = 709
Score = 33.1 bits (74), Expect = 1.3
Identities = 18/61 (29%), Positives = 33/61 (53%), Gaps = 2/61 (3%)
Query: 149 SLCVHGFGFDPLTGDYKILRLSWLVSLQNPFYDP--HVRLFSLKTNSWKIIPTMPYALVF 206
S+ + +G + DYKI + + N P ++++++LKTNSW+II P L +
Sbjct: 125 SMPIPVYGAIVFSYDYKIYVIGGMNYSGNSLVPPVNYIQVYNLKTNSWQIIGNAPLRLAY 184
Query: 207 A 207
+
Sbjct: 185 S 185
>Y511_CLOAB (Q97LP3) Hypothetical UPF0042 protein CAC0511
Length = 294
Score = 32.7 bits (73), Expect = 1.7
Identities = 27/95 (28%), Positives = 41/95 (42%), Gaps = 7/95 (7%)
Query: 175 LQNPFYDPHVRLFSLKTNSWKIIPTMPYALVFAQTMGVL--VEDSIHWIMAKKLDGLHPS 232
L NPFY P ++ FS P Y + F QT + +E + +++ L
Sbjct: 190 LPNPFYIPELKRFSGIEK-----PVKDYVMSFDQTKEFVNKIEQLLKFLIPNYLKEGKRQ 244
Query: 233 LIVAFNLTLEIFNEVPLPDEIGEEEVNSNDSVEID 267
LIV+ T V + +EI E N ++V ID
Sbjct: 245 LIVSIGCTGGRHRSVTIANEIYERLKNDGETVNID 279
>MSP1_PLAF3 (P19598) Merozoite surface protein 1 precursor
(Merozoite surface antigens) (PMMSA) (P190)
Length = 1682
Score = 32.7 bits (73), Expect = 1.7
Identities = 32/112 (28%), Positives = 52/112 (45%), Gaps = 20/112 (17%)
Query: 311 VTSHLKSSSPLCYSSDGSKVLIEGIEVLLEVHHKKLFWYDLKTEQVS------YEEIPDF 364
+T+H+ + LC + G K LI+G E + E+ +K F++DL +++ Y +IP F
Sbjct: 138 LTNHMLT---LCDNIHGFKYLIDGYEEINELLYKLNFYFDLLRAKLNDVCANDYCQIP-F 193
Query: 365 N----------GAKICVGSLVPPSFPVDNSSKKENHTCKSKKRYLLIIEFFQ 406
N K+ G P F DN K E++ K+K I E +
Sbjct: 194 NLKIRANELDVLKKLVFGYRKPLDFIKDNVGKMEDYIKKNKTTIANINELIE 245
>RPC1_PLAFA (P27625) DNA-directed RNA polymerase III largest subunit
(EC 2.7.7.6)
Length = 2339
Score = 32.3 bits (72), Expect = 2.3
Identities = 27/115 (23%), Positives = 54/115 (46%), Gaps = 7/115 (6%)
Query: 16 SRLPVKSLLRFRSTSKSLKSIIDSHNFINLHRKNSLNRSFILRLRSNIYQIEDDFSNLTT 75
+++P ++ ++ + ++ +I ++ N IN N NRS I SN+ I ++ SN+ +
Sbjct: 1054 NKIPYLPHVQHQNKTSNMNNIYNNMNNIN---NNDSNRSIIYNNDSNMNNINNNDSNMNS 1110
Query: 76 AVPLNHPFTRNSTNIALIGSCNGLLAVSNGEIALRHPNAANEITIWNPNIRKHHI 130
H N NI S ++ ++ + H N +N +I N N ++I
Sbjct: 1111 I----HNNNSNMNNIHNNDSNRSIIHNNDSNMNSIHNNDSNMNSIHNNNSNMNNI 1161
>MSP1_PLAFM (P08569) Merozoite surface protein 1 precursor
(Merozoite surface antigens) (PMMSA) (P190)
Length = 1701
Score = 32.0 bits (71), Expect = 3.0
Identities = 34/112 (30%), Positives = 51/112 (45%), Gaps = 20/112 (17%)
Query: 311 VTSHLKSSSPLCYSSDGSKVLIEGIEVLLEVHHKKLFWYDLKTEQV------SYEEIPDF 364
+T+H+ + S + DG K LI+G E + E+ +K F+YDL ++ SY +IP F
Sbjct: 144 LTNHMLTLSK---NVDGFKYLIDGYEEINELLYKLNFYYDLLRAKLNDACANSYCQIP-F 199
Query: 365 N----------GAKICVGSLVPPSFPVDNSSKKENHTCKSKKRYLLIIEFFQ 406
N KI G P DN K E++ K+K I E +
Sbjct: 200 NLKIRANELDVLKKIVFGYRKPLDNIKDNVGKMEDYIKKNKTTIANINELIE 251
>MSP1_PLAFF (P13819) Merozoite surface protein 1 precursor
(Merozoite surface antigens) (PMMSA)
Length = 1701
Score = 32.0 bits (71), Expect = 3.0
Identities = 34/112 (30%), Positives = 51/112 (45%), Gaps = 20/112 (17%)
Query: 311 VTSHLKSSSPLCYSSDGSKVLIEGIEVLLEVHHKKLFWYDLKTEQV------SYEEIPDF 364
+T+H+ + S + DG K LI+G E + E+ +K F+YDL ++ SY +IP F
Sbjct: 144 LTNHMLTLSK---NVDGFKYLIDGYEEINELLYKLNFYYDLLRAKLNDACANSYCQIP-F 199
Query: 365 N----------GAKICVGSLVPPSFPVDNSSKKENHTCKSKKRYLLIIEFFQ 406
N KI G P DN K E++ K+K I E +
Sbjct: 200 NLKIRANELDVLKKIVFGYRKPLDNIKDNVGKMEDYIKKNKTTIANINELIE 251
>SYA_BUCBP (P59420) Alanyl-tRNA synthetase (EC 6.1.1.7)
(Alanine--tRNA ligase) (AlaRS)
Length = 880
Score = 31.6 bits (70), Expect = 3.9
Identities = 14/41 (34%), Positives = 27/41 (65%), Gaps = 2/41 (4%)
Query: 35 SIIDSHNFINLHRKNSLNRSFILRLRSNIYQIEDDFSNLTT 75
+I++ ++N H++NS+N++F+ N Y IED+ N+ T
Sbjct: 422 NIMEFKRYLNQHKQNSINKNFL--NTRNAYYIEDNNINIKT 460
>R8L2_ARATH (Q9MAG6) Probable disease resistance RPP8-like protein 2
Length = 1271
Score = 31.6 bits (70), Expect = 3.9
Identities = 25/103 (24%), Positives = 51/103 (49%), Gaps = 15/103 (14%)
Query: 155 FGFDPLTGDYKILRLSWLVSLQNPFYDPHV-RLFSLKTNS--WKII--PTMPYALVFAQT 209
FGFDP+T +K+L ++W + + P+ ++ +L+T W+ I P +P+ F +
Sbjct: 1042 FGFDPITKQFKVLCMTW-----SRYGTPNTHQVLTLETGKRLWRTIQDPILPHYRSFDR- 1095
Query: 210 MGVLVEDSIHWIMAKKLDGLHPSLIVAFNLTLEIFNEVPLPDE 252
+ + +++ + S IV F+ E F+ + + DE
Sbjct: 1096 --ICINGVLYY--GADFEESQSSKIVCFDFRFEKFSFINIADE 1134
>DPOQ_HUMAN (O75417) DNA polymerase theta (EC 2.7.7.7) (DNA polymerase
eta)
Length = 1762
Score = 31.6 bits (70), Expect = 3.9
Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
Query: 141 RSPSDMNCSLCVHGFGFDPLTGDYKILRLSWLVSLQNPFYDPHVRLFSLKTNS 193
R SD CS+ ++ F YKIL LS +SL+ + DP V + L +S
Sbjct: 1125 RKESDKECSVVIYDF-----IQSYKILLLSCGISLEQSYEDPKVACWLLDPDS 1172
>MSP1_PLAFW (P04933) Merozoite surface protein 1 precursor
(Merozoite surface antigens) (PMMSA) (P195)
Length = 1639
Score = 30.8 bits (68), Expect = 6.6
Identities = 30/102 (29%), Positives = 49/102 (47%), Gaps = 20/102 (19%)
Query: 311 VTSHLKSSSPLCYSSDGSKVLIEGIEVLLEVHHKKLFWYDLKTEQVS------YEEIPDF 364
+T+H+ + LC + G K LI+G E + E+ +K F++DL +++ Y +IP F
Sbjct: 150 LTNHMLT---LCDNIHGFKYLIDGYEEINELLYKLNFYFDLLRAKLNDVCANDYCQIP-F 205
Query: 365 N----------GAKICVGSLVPPSFPVDNSSKKENHTCKSKK 396
N K+ G P DN K E++ K+KK
Sbjct: 206 NLKIRANELDVLKKLVFGYRKPLDNIKDNVGKMEDYIKKNKK 247
>MSP1_PLAFK (P04932) Merozoite surface protein 1 precursor
(Merozoite surface antigens) (PMMSA) (P190)
Length = 1630
Score = 30.8 bits (68), Expect = 6.6
Identities = 30/102 (29%), Positives = 49/102 (47%), Gaps = 20/102 (19%)
Query: 311 VTSHLKSSSPLCYSSDGSKVLIEGIEVLLEVHHKKLFWYDLKTEQVS------YEEIPDF 364
+T+H+ + LC + G K LI+G E + E+ +K F++DL +++ Y +IP F
Sbjct: 141 LTNHMLT---LCDNIHGFKYLIDGYEEINELLYKLNFYFDLLRAKLNDVCANDYCQIP-F 196
Query: 365 N----------GAKICVGSLVPPSFPVDNSSKKENHTCKSKK 396
N K+ G P DN K E++ K+KK
Sbjct: 197 NLKIRANELDVLKKLVFGYRKPLDNIKDNVGKMEDYIKKNKK 238
>KTHY_STRPY (Q9A190) Thymidylate kinase (EC 2.7.4.9) (dTMP kinase)
Length = 211
Score = 30.4 bits (67), Expect = 8.6
Identities = 16/55 (29%), Positives = 26/55 (47%), Gaps = 10/55 (18%)
Query: 213 LVEDSIHWIMAKKLDGLHPSLIVAFNLTLEIFNEVPLPDEIGEEEVNSNDSVEID 267
L++ I W+ DGL P L + F+ +P EIG +N+N E++
Sbjct: 113 LIKADIQWLNEFATDGLEPDLTLYFD----------VPSEIGLARINANQQREVN 157
>KTHY_STRP8 (Q8P2B1) Thymidylate kinase (EC 2.7.4.9) (dTMP kinase)
Length = 211
Score = 30.4 bits (67), Expect = 8.6
Identities = 16/55 (29%), Positives = 26/55 (47%), Gaps = 10/55 (18%)
Query: 213 LVEDSIHWIMAKKLDGLHPSLIVAFNLTLEIFNEVPLPDEIGEEEVNSNDSVEID 267
L++ I W+ DGL P L + F+ +P EIG +N+N E++
Sbjct: 113 LIKADIQWLNEFATDGLEPDLTLYFD----------VPSEIGLARINANQQREVN 157
>KTHY_STRP3 (Q8K8H4) Thymidylate kinase (EC 2.7.4.9) (dTMP kinase)
Length = 211
Score = 30.4 bits (67), Expect = 8.6
Identities = 16/55 (29%), Positives = 26/55 (47%), Gaps = 10/55 (18%)
Query: 213 LVEDSIHWIMAKKLDGLHPSLIVAFNLTLEIFNEVPLPDEIGEEEVNSNDSVEID 267
L++ I W+ DGL P L + F+ +P EIG +N+N E++
Sbjct: 113 LIKADIQWLNEFATDGLEPDLTLYFD----------VPSEIGLARINANQQREVN 157
>EPD2_SALSA (P28772) Ependymin II precursor (EPD-II)
Length = 221
Score = 30.4 bits (67), Expect = 8.6
Identities = 23/85 (27%), Positives = 35/85 (41%), Gaps = 7/85 (8%)
Query: 278 TVNYETTKIDVWVMKQYGLKDSWCKLFTMMKSCVTSHLKSSSPLCYSSDGSKVLIEGIEV 337
TV E K+++W+ K + L T+ + S YS+D +L EV
Sbjct: 130 TVQEENIKVNIWMGSVAETKGQYSVLTTVGECLPLSTF-------YSTDSITLLFSNSEV 182
Query: 338 LLEVHHKKLFWYDLKTEQVSYEEIP 362
+ EV ++F E V EE P
Sbjct: 183 VTEVKAPEMFTLPSFCEAVELEETP 207
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.138 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,805,280
Number of Sequences: 164201
Number of extensions: 2130886
Number of successful extensions: 4782
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 4776
Number of HSP's gapped (non-prelim): 19
length of query: 412
length of database: 59,974,054
effective HSP length: 113
effective length of query: 299
effective length of database: 41,419,341
effective search space: 12384382959
effective search space used: 12384382959
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 67 (30.4 bits)
Medicago: description of AC149495.3