BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149494.6 + phase: 0 /pseudo
(556 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BIOC_ERWHE (O06898) Biotin synthesis protein bioC 38 0.079
BIOC_SERMA (P36571) Biotin synthesis protein bioC 34 1.1
Y829_SYNY3 (Q55423) Putative methyltransferase sll0829 (EC 2.1.1.-) 33 1.5
POLS_ONNVG (P22056) Structural polyprotein (P130) [Contains: Coa... 32 4.3
YC33_METJA (Q58630) Hypothetical protein MJ1233 32 5.7
YAFE_ECOLI (P30866) Hypothetical protein yafE 32 5.7
UBIE_BACAN (Q81SW0) Menaquinone biosynthesis methyltransferase u... 31 7.4
TPS1_CANAL (Q92410) Alpha,alpha-trehalose-phosphate synthase [UD... 31 7.4
GTOM_ARATH (Q9ZSK1) Tocopherol O-methyltransferase, chloroplast ... 31 7.4
UBIE_TROWT (P67064) Menaquinone biosynthesis methyltransferase u... 31 9.7
UBIE_TROW8 (P67065) Menaquinone biosynthesis methyltransferase u... 31 9.7
UBIE_MICLU (O66128) Menaquinone biosynthesis methyltransferase u... 31 9.7
SYGB_VIBVU (Q8DDJ7) Glycyl-tRNA synthetase beta chain (EC 6.1.1.... 31 9.7
SYGB_VIBPA (Q87TP8) Glycyl-tRNA synthetase beta chain (EC 6.1.1.... 31 9.7
>BIOC_ERWHE (O06898) Biotin synthesis protein bioC
Length = 251
Score = 37.7 bits (86), Expect = 0.079
Identities = 30/96 (31%), Positives = 42/96 (43%), Gaps = 8/96 (8%)
Query: 217 TALDTGCGVASWGAYLQSRN--IITLSLAPRDTHEAQVQFALERGVPALIGVLASKRLPF 274
+ LD GCG S Y + + L L+ +AQ Q A + V A I + LP
Sbjct: 45 SVLDAGCGPGSMSRYWRDAGAEVTALDLSLPMLRQAQSQQAAQHYVAADI-----EALPL 99
Query: 275 PSRAFDISHCSRCLIPWAEYDGIFLNEVDRVLRPGG 310
FD++ S + W G L + RV+RPGG
Sbjct: 100 ADARFDLAW-SNLAVQWCNDLGQALKSLHRVVRPGG 134
>BIOC_SERMA (P36571) Biotin synthesis protein bioC
Length = 255
Score = 33.9 bits (76), Expect = 1.1
Identities = 30/99 (30%), Positives = 44/99 (44%), Gaps = 8/99 (8%)
Query: 219 LDTGCGVASWGAYLQSRN--IITLSLAPRDTHEAQVQFALERGVPALIGVLASKRLPFPS 276
LD GCG + + R + L LAP A+ + A L+G + +++P P
Sbjct: 51 LDAGCGTGYFSRMWRERGKRVTALDLAPGMLDVARQRQAAHH---YLLGDI--EQVPLPD 105
Query: 277 RAFDISHCSRCLIPWAEYDGIFLNEVDRVLRPGGYWILS 315
A DI S ++ W L E+ RV RPGG + S
Sbjct: 106 AAMDICFSS-LVVQWCSDLPAALAELYRVTRPGGVILFS 143
>Y829_SYNY3 (Q55423) Putative methyltransferase sll0829 (EC 2.1.1.-)
Length = 212
Score = 33.5 bits (75), Expect = 1.5
Identities = 35/121 (28%), Positives = 51/121 (41%), Gaps = 12/121 (9%)
Query: 207 LINLKDGSIRTALDTGCGVASWGAYL--QSRNIITLSLAPRDTHEAQVQFALERGVPALI 264
L NL + LD CG YL ++ L +P+ A++ V L
Sbjct: 38 LENLAISPGQKVLDLCCGGGQATVYLAQSGATVVGLDASPKALGRAKINVPQATYVQGL- 96
Query: 265 GVLASKRLPFPSRAFDISHCSRCL--IPWAEYDGIFLNEVDRVLRPGGYWILSG--PPIN 320
++ LPF FD+ H S L + A+ I ++ V RVL+PGG + L P N
Sbjct: 97 ----AEDLPFGEGEFDLVHTSVALHEMTPAQLQSI-ISGVHRVLKPGGIFALVDLHRPSN 151
Query: 321 W 321
W
Sbjct: 152 W 152
>POLS_ONNVG (P22056) Structural polyprotein (P130) [Contains: Coat
protein C (EC 3.4.21.-) (Capsid protein C); Spike
glycoprotein E3; Spike glycoprotein E2; 6 kDa peptide;
Spike glycoprotein E1]
Length = 1247
Score = 32.0 bits (71), Expect = 4.3
Identities = 34/144 (23%), Positives = 59/144 (40%), Gaps = 23/144 (15%)
Query: 175 NW----IRYDGDRFFFPGGGTMFPNGAGAYIDDIGKLINLKDGSIRTALDTGCGVASWGA 230
NW ++Y G RF P G + D+ G+++ + G T V +W
Sbjct: 187 NWHHGAVQYSGGRFTIPTGAGKPGDSGRPIFDNKGRVVAIVLGGANEGTRTALSVVTW-- 244
Query: 231 YLQSRNIITLSLAPRDTHEAQVQFALERGVPALIGVLASKRLPFPSRAFDISHCSRCLIP 290
+++I+T + T E V+++L V L LA+ P C+ C
Sbjct: 245 ---NKDIVT-----KITPEGSVEWSLALPVMCL---LANTTFPCSQ-----PPCAPCCYE 288
Query: 291 WAEYDGIFLNEVDRVLRPGGYWIL 314
+ + + E D V++PG Y +L
Sbjct: 289 KKPEETLRMLE-DNVMQPGYYQLL 311
>YC33_METJA (Q58630) Hypothetical protein MJ1233
Length = 288
Score = 31.6 bits (70), Expect = 5.7
Identities = 32/119 (26%), Positives = 47/119 (38%), Gaps = 8/119 (6%)
Query: 200 YIDDIGKLINLKDGSIRTALDT--GCGVASWGAYLQSRNIITLSLAPRDTHEAQVQFALE 257
Y D + K+ +K LDT G G + AY + +IT+ P A++ E
Sbjct: 120 YEDTLNKINAVKVKKGEKVLDTCMGLGYTAIEAYRRGAEVITIEKNPNVLELAKINPYSE 179
Query: 258 R----GVPALIGVLASKRLPFPSRAFD--ISHCSRCLIPWAEYDGIFLNEVDRVLRPGG 310
G+ ++G F FD I R + Y F E+ RVL+PGG
Sbjct: 180 ELFKGGIKIILGDAYDVIKRFKDEEFDVVIHDPPRFSLAGHLYSEEFYKEIFRVLKPGG 238
>YAFE_ECOLI (P30866) Hypothetical protein yafE
Length = 207
Score = 31.6 bits (70), Expect = 5.7
Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Query: 269 SKRLPFPSRAFDISHCSRCLIPWAEYDGIFLNEVDRVLRPGGYWIL 314
++ LPF AFDI W + G L EV+R+L+PGG I+
Sbjct: 52 AESLPFADNAFDIVISRYSAHHWHDV-GAALREVNRILKPGGRLIV 96
>UBIE_BACAN (Q81SW0) Menaquinone biosynthesis methyltransferase ubiE
(EC 2.1.1.-)
Length = 237
Score = 31.2 bits (69), Expect = 7.4
Identities = 31/112 (27%), Positives = 50/112 (43%), Gaps = 12/112 (10%)
Query: 206 KLINLKDGSIRTALDTGCGVASW-----GAYLQSRNIITLSLAPR--DTHEAQVQFALER 258
+++++K GS ALD CG A W GA + ++ L + + +V+ +
Sbjct: 41 RIMDVKPGS--KALDVCCGTADWTIALAGAVGEQGKVVGLDFSENMLSVGKQKVEALQLK 98
Query: 259 GVPALIGVLASKRLPFPSRAFDISHCSRCLIPWAEYDGIFLNEVDRVLRPGG 310
V L G + LPF FD L +Y + L E+ RV++PGG
Sbjct: 99 QVELLHG--NAMELPFEDNTFDYVTIGFGLRNVPDYMHV-LKEMTRVVKPGG 147
>TPS1_CANAL (Q92410) Alpha,alpha-trehalose-phosphate synthase
[UDP-forming] (EC 2.4.1.15) (Trehalose-6-phosphate
synthase) (UDP-glucose-glucosephosphate
glucosyltransferase)
Length = 478
Score = 31.2 bits (69), Expect = 7.4
Identities = 33/117 (28%), Positives = 47/117 (39%), Gaps = 12/117 (10%)
Query: 445 EGYSKDN--ELWKKRIPHYKKVNNQLGTKRYRNLVDMNANLGGFASALVKNPVWVMNVVP 502
+G KD+ E K+ +K V +G R + + L F L +NP W+ VV
Sbjct: 251 DGLKKDSVVERIKQLKSKFKDVKVIVGVDRLDYIKGVPQKLHAFEVFLNENPEWIGKVVL 310
Query: 503 VQAKVDTLGAIYE--------RGLIGTYHDWCEAMSTYPRTY--DLIHADSLFSLYN 549
VQ V + G + E L+G + + P Y I D L SLYN
Sbjct: 311 VQVAVPSRGDVEEYQSLRSTVSELVGRINGEFGTVEFVPIHYLHKSIPFDELISLYN 367
>GTOM_ARATH (Q9ZSK1) Tocopherol O-methyltransferase, chloroplast
precursor (EC 2.1.1.95) (Gamma-tocopherol
methyltransferase)
Length = 348
Score = 31.2 bits (69), Expect = 7.4
Identities = 39/161 (24%), Positives = 62/161 (38%), Gaps = 26/161 (16%)
Query: 211 KDGSIRTALDTGCGVASWGAYLQSR---NIITLSLAPRDTHEA-QVQFALERGVPALIGV 266
++ I+ +D GCG+ YL S+ I ++L+P A + A A V
Sbjct: 123 EEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAAQSLSHKASFQV 182
Query: 267 LASKRLPFPSRAFDISHCSRCLIPWAEYDG-------IFLNEVDRVLRPGGYWILSGPPI 319
+ PF FD + W+ G F+ E+ RV PGG I+ +
Sbjct: 183 ADALDQPFEDGKFD--------LVWSMESGEHMPDKAKFVKELVRVAAPGGRIII----V 230
Query: 320 NWNKHHRGWQRTKKDLNQ-EQTKIEKVAKSLCWNKLIEKDD 359
W HR ++ L EQ ++K+ K+ DD
Sbjct: 231 TW--CHRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDD 269
>UBIE_TROWT (P67064) Menaquinone biosynthesis methyltransferase ubiE
(EC 2.1.1.-)
Length = 239
Score = 30.8 bits (68), Expect = 9.7
Identities = 18/44 (40%), Positives = 24/44 (53%), Gaps = 1/44 (2%)
Query: 267 LASKRLPFPSRAFDISHCSRCLIPWAEYDGIFLNEVDRVLRPGG 310
L + LPFP ++FD CL + G FL + RVL+PGG
Sbjct: 109 LTNGDLPFPDKSFDAILMVFCLRNVSNIQG-FLCDAARVLKPGG 151
>UBIE_TROW8 (P67065) Menaquinone biosynthesis methyltransferase ubiE
(EC 2.1.1.-)
Length = 239
Score = 30.8 bits (68), Expect = 9.7
Identities = 18/44 (40%), Positives = 24/44 (53%), Gaps = 1/44 (2%)
Query: 267 LASKRLPFPSRAFDISHCSRCLIPWAEYDGIFLNEVDRVLRPGG 310
L + LPFP ++FD CL + G FL + RVL+PGG
Sbjct: 109 LTNGDLPFPDKSFDAILMVFCLRNVSNIQG-FLCDAARVLKPGG 151
>UBIE_MICLU (O66128) Menaquinone biosynthesis methyltransferase ubiE
(EC 2.1.1.-)
Length = 246
Score = 30.8 bits (68), Expect = 9.7
Identities = 31/110 (28%), Positives = 48/110 (43%), Gaps = 10/110 (9%)
Query: 206 KLINLKDGSIRTALDTGCGVASWGAYL-----QSRNIITLSLAPRDTHEAQVQFALERGV 260
K +N+K GS ALD CG W + ++ ++I L + AQ + + +
Sbjct: 52 KQMNVKKGS--KALDVCCGTGDWTIQMAQAVGKNGHVIGLDFSENMLSVAQGKTNHIQNI 109
Query: 261 PALIGVLASKRLPFPSRAFDISHCSRCLIPWAEYDGIFLNEVDRVLRPGG 310
+ G + LPF FD + L +Y L E+ RVL+PGG
Sbjct: 110 ELIHG--NAMELPFEDNIFDYTTIGFGLRNLPDYKK-GLEEMYRVLKPGG 156
>SYGB_VIBVU (Q8DDJ7) Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)
(Glycine--tRNA ligase beta chain) (GlyRS)
Length = 693
Score = 30.8 bits (68), Expect = 9.7
Identities = 22/77 (28%), Positives = 35/77 (44%), Gaps = 9/77 (11%)
Query: 247 THEAQVQFALERGVPALIGVLAS--------KRLPFPSRAFDIS-HCSRCLIPWAEYDGI 297
THE +A R + + LA KR P S AFD + ++ WA GI
Sbjct: 40 THEGVKWYAAPRRLALKVTALAEHQADKIVEKRGPAVSAAFDADGNATKAAQGWARGCGI 99
Query: 298 FLNEVDRVLRPGGYWIL 314
+++ +R++ G W+L
Sbjct: 100 TVDQAERMITDKGEWLL 116
>SYGB_VIBPA (Q87TP8) Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)
(Glycine--tRNA ligase beta chain) (GlyRS)
Length = 688
Score = 30.8 bits (68), Expect = 9.7
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 9/76 (11%)
Query: 248 HEAQVQFALERGVPALIGVLAS--------KRLPFPSRAFDIS-HCSRCLIPWAEYDGIF 298
HE FA R + + LA KR P S AFD + ++ WA GI
Sbjct: 41 HEGVKWFAAPRRLALKVAALAESQSDKVVEKRGPAVSAAFDAEGNPTKAAQGWARGCGIT 100
Query: 299 LNEVDRVLRPGGYWIL 314
+++ DR++ G W+L
Sbjct: 101 VDQADRMVTDKGEWLL 116
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.137 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,003,651
Number of Sequences: 164201
Number of extensions: 3317016
Number of successful extensions: 6364
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 6361
Number of HSP's gapped (non-prelim): 14
length of query: 556
length of database: 59,974,054
effective HSP length: 115
effective length of query: 441
effective length of database: 41,090,939
effective search space: 18121104099
effective search space used: 18121104099
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 68 (30.8 bits)
Medicago: description of AC149494.6