BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149305.1 + phase: 0 /pseudo
(146 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPEN_DROME (Q8SX83) Split ends protein 32 0.37
ATPB_THIFE (P41168) ATP synthase beta chain (EC 3.6.3.14) 32 0.63
ATPB_ECOLI (P00824) ATP synthase beta chain (EC 3.6.3.14) 32 0.63
RLM1_YEAST (Q12224) Transcription factor RLM1 31 0.82
PAO_ARATH (Q9FYC2) Pheophorbide a oxygenase, chloroplast precurs... 31 0.82
ATPB_BURCE (P42468) ATP synthase beta chain (EC 3.6.3.14) 31 0.82
ZW10_HUMAN (O43264) Centromere/kinetochore protein zw10 homolog 31 1.1
ATPB_BUCAP (Q07232) ATP synthase beta chain (EC 3.6.3.14) 31 1.1
ATPB_BUCAI (P57124) ATP synthase beta chain (EC 3.6.3.14) 31 1.1
IF5_YEAST (P38431) Eukaryotic translation initiation factor 5 (e... 30 1.8
ATPB_HAEIN (P43715) ATP synthase beta chain (EC 3.6.3.14) 30 1.8
CUPP_DROME (P14484) Pupal cuticle protein precursor 30 2.4
ATPB_VIBCH (Q9KNH5) ATP synthase beta chain (EC 3.6.3.14) 29 3.1
ATPB_PASMU (Q9CKW1) ATP synthase beta chain (EC 3.6.3.14) 29 3.1
YUBO_CAEEL (Q93591) Hypothetical protein F26A3.7 in chromosome I 29 4.1
YKD1_CAEEL (Q03560) Hypothetical protein B0464.2 in chromosome III 29 4.1
URID_SOYBN (O04104) Uricase II isozyme 2 (EC 1.7.3.3) (Urate oxi... 29 4.1
PHFE_HUMAN (O94880) PHD finger protein 14 29 4.1
DAB_DROME (P98081) Disabled protein 29 4.1
SNWA_DICDI (P54705) Protein snwA 28 5.3
>SPEN_DROME (Q8SX83) Split ends protein
Length = 5560
Score = 32.3 bits (72), Expect = 0.37
Identities = 20/62 (32%), Positives = 32/62 (51%), Gaps = 5/62 (8%)
Query: 26 SCIVSMMLDVSEPPKPGDVFSRQSIPFNIGESQFSLPTSPQELIQRYQEFKNALKEDTVD 85
S +VS ++ S G V + S+P + S SLP SP L QR++ +NA + D
Sbjct: 906 SAVVSCNVNAS-----GTVPASTSMPSGVSSSSSSLPMSPAALAQRHRMVRNARQTVDCD 960
Query: 86 YS 87
++
Sbjct: 961 FN 962
>ATPB_THIFE (P41168) ATP synthase beta chain (EC 3.6.3.14)
Length = 468
Score = 31.6 bits (70), Expect = 0.63
Identities = 14/39 (35%), Positives = 24/39 (60%)
Query: 41 PGDVFSRQSIPFNIGESQFSLPTSPQELIQRYQEFKNAL 79
P D FSRQ P +G+ + + S Q+ +QRY+E ++ +
Sbjct: 342 PLDSFSRQLDPQIVGQEHYDVARSCQKTLQRYKELQDII 380
>ATPB_ECOLI (P00824) ATP synthase beta chain (EC 3.6.3.14)
Length = 459
Score = 31.6 bits (70), Expect = 0.63
Identities = 14/39 (35%), Positives = 21/39 (52%)
Query: 41 PGDVFSRQSIPFNIGESQFSLPTSPQELIQRYQEFKNAL 79
P D SRQ P +G+ + Q ++QRYQE K+ +
Sbjct: 336 PLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDII 374
>RLM1_YEAST (Q12224) Transcription factor RLM1
Length = 676
Score = 31.2 bits (69), Expect = 0.82
Identities = 25/91 (27%), Positives = 38/91 (41%), Gaps = 13/91 (14%)
Query: 67 ELIQRYQEFKNALKE--DTVDYSLY--------TANIKRKRPTQTPRKVRKSGPPMVGGD 116
+LI YQ KN L E D DY + ++ R + P K G D
Sbjct: 64 DLIYHYQNDKNLLHEVKDPSDYGDFHKSASVNINQDLLRSSMSNKPSKSNVKGMNQSEND 123
Query: 117 NDE---DDDEDWAGGSRNISFSGGRRSSLRN 144
+DE +DD+D RN + +++S +N
Sbjct: 124 DDENNDEDDDDHGNFERNSNMHSNKKASDKN 154
>PAO_ARATH (Q9FYC2) Pheophorbide a oxygenase, chloroplast precursor
(EC 1.-.-.-) (Pheide a oxygenase) (AtPaO) (Accelerated
cell death 1) (Lethal leaf-spot 1 homolog) (Lls1)
Length = 537
Score = 31.2 bits (69), Expect = 0.82
Identities = 22/84 (26%), Positives = 36/84 (42%), Gaps = 10/84 (11%)
Query: 37 EPPKPGDVFSRQSIPFNIGESQFSLPTSPQELIQRYQEF-KNALKEDTVDYSLYTANIKR 95
EPP+ +P + + +FS T ++L Y +N +D++ + +R
Sbjct: 214 EPPR---------LPDDFDKPEFSTVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRR 264
Query: 96 KRPTQTPRKVRKSGPPMVGGDNDE 119
R P KV SGP G ND+
Sbjct: 265 DRAKPLPFKVESSGPWGFQGANDD 288
>ATPB_BURCE (P42468) ATP synthase beta chain (EC 3.6.3.14)
Length = 463
Score = 31.2 bits (69), Expect = 0.82
Identities = 15/39 (38%), Positives = 23/39 (58%)
Query: 41 PGDVFSRQSIPFNIGESQFSLPTSPQELIQRYQEFKNAL 79
P D SRQ P IGE +S+ Q+ +QRY+E ++ +
Sbjct: 339 PLDSTSRQIDPNVIGEEHYSITRRVQQTLQRYKELRDII 377
>ZW10_HUMAN (O43264) Centromere/kinetochore protein zw10 homolog
Length = 778
Score = 30.8 bits (68), Expect = 1.1
Identities = 19/47 (40%), Positives = 26/47 (54%), Gaps = 12/47 (25%)
Query: 59 FSLPTSP------QELIQRYQEFKNALKE------DTVDYSLYTANI 93
+S+PT+ +E+IQ +EF+NALKE DT D Y NI
Sbjct: 348 YSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNI 394
>ATPB_BUCAP (Q07232) ATP synthase beta chain (EC 3.6.3.14)
Length = 466
Score = 30.8 bits (68), Expect = 1.1
Identities = 12/34 (35%), Positives = 20/34 (58%)
Query: 46 SRQSIPFNIGESQFSLPTSPQELIQRYQEFKNAL 79
SRQ P+ +G+ + Q ++QRYQE K+ +
Sbjct: 348 SRQLDPYIVGDEHYDTARGVQSILQRYQELKDII 381
>ATPB_BUCAI (P57124) ATP synthase beta chain (EC 3.6.3.14)
Length = 465
Score = 30.8 bits (68), Expect = 1.1
Identities = 12/34 (35%), Positives = 20/34 (58%)
Query: 46 SRQSIPFNIGESQFSLPTSPQELIQRYQEFKNAL 79
SRQ P+ +G+ + Q ++QRYQE K+ +
Sbjct: 347 SRQLDPYIVGDEHYETALGVQSILQRYQELKDII 380
>IF5_YEAST (P38431) Eukaryotic translation initiation factor 5
(eIF-5)
Length = 405
Score = 30.0 bits (66), Expect = 1.8
Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 9/69 (13%)
Query: 61 LPTSPQELIQRYQEFKNALKEDTVDYSLYTANIKRKRPTQTPRKVRKSGPPMV------G 114
+P P+ L+Q Y + + E+ + T + K+ P + +KVR++ P +
Sbjct: 340 IPLLPKILVQLYNN--DIISEEEI-MRFGTKSSKKFVPKEVSKKVRRAAKPFITWLETAE 396
Query: 115 GDNDEDDDE 123
D+DE+DDE
Sbjct: 397 SDDDEEDDE 405
>ATPB_HAEIN (P43715) ATP synthase beta chain (EC 3.6.3.14)
Length = 457
Score = 30.0 bits (66), Expect = 1.8
Identities = 13/39 (33%), Positives = 22/39 (56%)
Query: 41 PGDVFSRQSIPFNIGESQFSLPTSPQELIQRYQEFKNAL 79
P D SRQ P +G+ + + Q ++QRY+E K+ +
Sbjct: 334 PLDSTSRQLDPLVVGQEHYDVARGVQGILQRYKELKDII 372
>CUPP_DROME (P14484) Pupal cuticle protein precursor
Length = 184
Score = 29.6 bits (65), Expect = 2.4
Identities = 17/67 (25%), Positives = 32/67 (47%), Gaps = 5/67 (7%)
Query: 50 IPFNIGESQFSLPTSPQELIQRYQEFKNALKEDTVDYSLYTANIK-----RKRPTQTPRK 104
+P I S + T P ++ Y ++ D +++YT NI+ + RP+ TP+
Sbjct: 102 VPDYILRSLEYIRTHPYQIKDYYTGELKTVEHDAAAFNVYTRNIQDHTIPQSRPSTTPKT 161
Query: 105 VRKSGPP 111
+ + PP
Sbjct: 162 IYLTHPP 168
>ATPB_VIBCH (Q9KNH5) ATP synthase beta chain (EC 3.6.3.14)
Length = 466
Score = 29.3 bits (64), Expect = 3.1
Identities = 13/39 (33%), Positives = 21/39 (53%)
Query: 41 PGDVFSRQSIPFNIGESQFSLPTSPQELIQRYQEFKNAL 79
P D SRQ P +G+ + + Q +QRY+E K+ +
Sbjct: 343 PLDSTSRQLDPLVVGQEHYDVARGVQATLQRYKELKDII 381
>ATPB_PASMU (Q9CKW1) ATP synthase beta chain (EC 3.6.3.14)
Length = 457
Score = 29.3 bits (64), Expect = 3.1
Identities = 13/39 (33%), Positives = 22/39 (56%)
Query: 41 PGDVFSRQSIPFNIGESQFSLPTSPQELIQRYQEFKNAL 79
P + SRQ P +GE +++ Q +QRY+E K+ +
Sbjct: 334 PLESTSRQLDPLVVGEEHYNVARGVQTTLQRYKELKDII 372
>YUBO_CAEEL (Q93591) Hypothetical protein F26A3.7 in chromosome I
Length = 297
Score = 28.9 bits (63), Expect = 4.1
Identities = 16/59 (27%), Positives = 28/59 (47%)
Query: 62 PTSPQELIQRYQEFKNALKEDTVDYSLYTANIKRKRPTQTPRKVRKSGPPMVGGDNDED 120
P+ P+E+ ++ Q +N ++D D + + P+ V S P G D+DED
Sbjct: 61 PSIPKEVREKLQNVENPAEDDEEDEEFPSQSYGPSIPSNFRPTVGPSIPGTFGDDSDED 119
>YKD1_CAEEL (Q03560) Hypothetical protein B0464.2 in chromosome III
Length = 1150
Score = 28.9 bits (63), Expect = 4.1
Identities = 10/21 (47%), Positives = 16/21 (75%)
Query: 111 PMVGGDNDEDDDEDWAGGSRN 131
P++GG +D+DDD+ G SR+
Sbjct: 1113 PIIGGSDDDDDDKPAGGNSRD 1133
>URID_SOYBN (O04104) Uricase II isozyme 2 (EC 1.7.3.3) (Urate
oxidase) (Nodulin 35) (N-35) (Non-symbiotic uricase)
Length = 309
Score = 28.9 bits (63), Expect = 4.1
Identities = 12/26 (46%), Positives = 16/26 (61%)
Query: 56 ESQFSLPTSPQELIQRYQEFKNALKE 81
ESQ+SLP P ++YQE K L +
Sbjct: 198 ESQYSLPQKPLYFTEKYQEVKKVLAD 223
>PHFE_HUMAN (O94880) PHD finger protein 14
Length = 888
Score = 28.9 bits (63), Expect = 4.1
Identities = 16/33 (48%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Query: 97 RPTQTPRKVRKSGPPMVGGDNDEDDDEDWAGGS 129
RPT RK R S G D D +DDED GS
Sbjct: 215 RPTVVKRKGR-SASQKEGSDGDNEDDEDEGSGS 246
>DAB_DROME (P98081) Disabled protein
Length = 2411
Score = 28.9 bits (63), Expect = 4.1
Identities = 20/78 (25%), Positives = 35/78 (44%), Gaps = 8/78 (10%)
Query: 62 PTSPQELIQRYQEFKNALKED-----TVDYSLYTANIKRKRPTQTPR--KVRKSGPPMVG 114
P ++L+Q + EF + ED +D+ + + + R S +
Sbjct: 1237 PEKEKDLLQDFPEFSDEFNEDHDLRQIMDHQNVQTHARDRHGLVDSRGFPTEPSSSALTV 1296
Query: 115 GDNDEDDDEDWAGGSRNI 132
GD+DED+D D AGG ++
Sbjct: 1297 GDDDEDEDAD-AGGESSL 1313
>SNWA_DICDI (P54705) Protein snwA
Length = 685
Score = 28.5 bits (62), Expect = 5.3
Identities = 16/55 (29%), Positives = 23/55 (41%)
Query: 70 QRYQEFKNALKEDTVDYSLYTANIKRKRPTQTPRKVRKSGPPMVGGDNDEDDDED 124
QRY N+ ++ D + K KR R+ R + ND DDD+D
Sbjct: 361 QRYTRKDNSDSDNDNDNDSSSDEDKNKRTPPMNRRSRSRSTERIPSRNDNDDDDD 415
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.329 0.143 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,045,740
Number of Sequences: 164201
Number of extensions: 751554
Number of successful extensions: 3827
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 3784
Number of HSP's gapped (non-prelim): 45
length of query: 146
length of database: 59,974,054
effective HSP length: 100
effective length of query: 46
effective length of database: 43,553,954
effective search space: 2003481884
effective search space used: 2003481884
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC149305.1