BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149197.7 - phase: 0
(218 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YKP5_YEAST (P36056) Hypothetical 72.2 kDa protein in RPS27A-GPM1... 36 0.061
LEU3_THEAQ (P24098) 3-isopropylmalate dehydrogenase (EC 1.1.1.85... 34 0.30
P29_ECHGR (Q9U8G7) Hydatid disease diagnostic antigen P-29 33 0.39
LEU3_THETH (P61495) 3-isopropylmalate dehydrogenase (EC 1.1.1.85... 33 0.39
LEU3_THET2 (P61494) 3-isopropylmalate dehydrogenase (EC 1.1.1.85... 33 0.39
ECE2_HUMAN (O60344) Endothelin-converting enzyme 2 (EC 3.4.24.71... 33 0.67
HS82_TOBAC (P36182) Heat shock protein 82 (Fragment) 31 2.0
ECE2_BOVIN (Q10711) Endothelin-converting enzyme 2 (EC 3.4.24.71... 31 2.0
CGB1_MOUSE (P24860) G2/mitotic-specific cyclin B1 31 2.0
NOT4_YEAST (P34909) General negative regulator of transcription ... 30 3.3
HEMZ_PSESM (Q888A2) Ferrochelatase (EC 4.99.1.1) (Protoheme ferr... 30 4.3
LAP1_HUMAN (Q96NW7) LAP1 protein (Densin-180) 30 5.7
HS83_IPONI (P51819) Heat shock protein 83 30 5.7
CPPM_BACHD (Q9Z9T7) Putative carboxyvinyl-carboxyphosphonate pho... 30 5.7
DCTD_RHILE (P10046) C4-dicarboxylate transport transcriptional r... 29 7.4
TP2A_MOUSE (Q01320) DNA topoisomerase II, alpha isozyme (EC 5.99... 29 9.7
LEU3_BUCAP (O85064) 3-isopropylmalate dehydrogenase (EC 1.1.1.85... 29 9.7
>YKP5_YEAST (P36056) Hypothetical 72.2 kDa protein in RPS27A-GPM1
intergenic region
Length = 628
Score = 36.2 bits (82), Expect = 0.061
Identities = 57/266 (21%), Positives = 98/266 (36%), Gaps = 71/266 (26%)
Query: 9 LVEVSRRI--DGLSPTKVLDFGAGTVSAFWALQEV----WPKSLEKVNIIEPSQSMQRAG 62
L E+ +R+ D P ++LD G G + AL ++ + L+ I+ ++ +RA
Sbjct: 147 LKELQKRVGPDNFKPQRILDVGYGPATGIVALNDILGPNYRPDLKDAVILGNAEMQERAK 206
Query: 63 QRLIQGLKDLPLIHSYDSIQALSKDISKSERGHD--------------LVIASYVLGEIP 108
L + L ++ + + + + K+ R + + I + + IP
Sbjct: 207 IILSRQLNEV--VDTVEENVSTEKEQETDRRNKNFQEDEHIGEVMTKKINIMTNLRSSIP 264
Query: 109 SIK--DRITILRQLWDQTQDV--------------------LVLVEPGTPHGSSIIAQMR 146
+ K D I + QL +V++E G P G IIA+ R
Sbjct: 265 ASKEYDLIILTHQLLHDGNQFPIQVDENIEHYLNILAPGGHIVIIERGNPMGFEIIARAR 324
Query: 147 SYILWMEE-------------RKHRKSSKKNNEVCKDLITEKAGAFVVAPCPHDGTCPLV 193
L E R KK+ E+ I+ V+APCPH CPL
Sbjct: 325 QITLRPENFPDEFGKIPRPWSRGVTVRGKKDAELGN--ISSNYFLKVIAPCPHQRKCPLQ 382
Query: 194 ------------KSGKYCHFVQRLER 207
K K+C+F + ++R
Sbjct: 383 VGNPNFYTHKEGKDLKFCNFQKSIKR 408
>LEU3_THEAQ (P24098) 3-isopropylmalate dehydrogenase (EC 1.1.1.85)
(Beta-IPM dehydrogenase) (IMDH) (3-IPM-DH)
Length = 344
Score = 33.9 bits (76), Expect = 0.30
Identities = 16/65 (24%), Positives = 36/65 (54%), Gaps = 2/65 (3%)
Query: 45 SLEKVNIIEPSQSMQRAGQRLIQGLKDLPLIHSYDSIQALSKDISKSERGHDLVIASYVL 104
S++K N++E + ++ + + +G D+ L H Y + A++ + K+ D+V+ +
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVHKGYPDVALDHQY--VDAMAMHLVKNPARFDVVVTGNIF 239
Query: 105 GEIPS 109
G+I S
Sbjct: 240 GDILS 244
>P29_ECHGR (Q9U8G7) Hydatid disease diagnostic antigen P-29
Length = 238
Score = 33.5 bits (75), Expect = 0.39
Identities = 27/99 (27%), Positives = 44/99 (44%), Gaps = 1/99 (1%)
Query: 74 LIHSYDSIQA-LSKDISKSERGHDLVIASYVLGEIPSIKDRITILRQLWDQTQDVLVLVE 132
LIH D ++A +SK I+ +E D+ IAS V K++IT +L + V E
Sbjct: 36 LIHEIDQMKAWISKIITATEEFVDINIASKVADAFQKNKEKITTTDKLGTALEQVASQSE 95
Query: 133 PGTPHGSSIIAQMRSYILWMEERKHRKSSKKNNEVCKDL 171
P S ++ + M + +S+ N +DL
Sbjct: 96 KAAPQLSKMLTEASDVHQRMATARKNFNSEVNTTFIEDL 134
>LEU3_THETH (P61495) 3-isopropylmalate dehydrogenase (EC 1.1.1.85)
(Beta-IPM dehydrogenase) (IMDH) (3-IPM-DH)
Length = 345
Score = 33.5 bits (75), Expect = 0.39
Identities = 16/65 (24%), Positives = 36/65 (54%), Gaps = 2/65 (3%)
Query: 45 SLEKVNIIEPSQSMQRAGQRLIQGLKDLPLIHSYDSIQALSKDISKSERGHDLVIASYVL 104
S++K N++E + ++ + + +G D+ L H Y + A++ + +S D+V+ +
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQY--VDAMAMHLVRSPARFDVVVTGNIF 239
Query: 105 GEIPS 109
G+I S
Sbjct: 240 GDILS 244
>LEU3_THET2 (P61494) 3-isopropylmalate dehydrogenase (EC 1.1.1.85)
(Beta-IPM dehydrogenase) (IMDH) (3-IPM-DH)
Length = 345
Score = 33.5 bits (75), Expect = 0.39
Identities = 16/65 (24%), Positives = 36/65 (54%), Gaps = 2/65 (3%)
Query: 45 SLEKVNIIEPSQSMQRAGQRLIQGLKDLPLIHSYDSIQALSKDISKSERGHDLVIASYVL 104
S++K N++E + ++ + + +G D+ L H Y + A++ + +S D+V+ +
Sbjct: 182 SVDKANVLEVGEFWRKTVEEVGRGYPDVALEHQY--VDAMAMHLVRSPARFDVVVTGNIF 239
Query: 105 GEIPS 109
G+I S
Sbjct: 240 GDILS 244
>ECE2_HUMAN (O60344) Endothelin-converting enzyme 2 (EC 3.4.24.71)
(ECE-2)
Length = 787
Score = 32.7 bits (73), Expect = 0.67
Identities = 22/116 (18%), Positives = 49/116 (41%), Gaps = 1/116 (0%)
Query: 81 IQALSKDISKSERGHDLVIASYVL-GEIPSIKDRITILRQLWDQTQDVLVLVEPGTPHGS 139
+++L + +S E + ++ +P + R LWDQ Q +L + T S
Sbjct: 129 LESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNS 188
Query: 140 SIIAQMRSYILWMEERKHRKSSKKNNEVCKDLITEKAGAFVVAPCPHDGTCPLVKS 195
S A+ ++ ++ + + + + +DLI + G + P D ++K+
Sbjct: 189 SSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGPWDQDNFMEVLKA 244
>HS82_TOBAC (P36182) Heat shock protein 82 (Fragment)
Length = 499
Score = 31.2 bits (69), Expect = 2.0
Identities = 46/198 (23%), Positives = 85/198 (42%), Gaps = 33/198 (16%)
Query: 40 EVWPKSLEKVNIIEPSQSMQRAGQRLIQGLKDLPLIHSY---DSIQALSKDISKSERGHD 96
E + K+L K+ I E SQ+ + L DL HS D + +L +++ + G
Sbjct: 211 EAFSKNL-KLGIHEDSQNRAK--------LADLLRYHSTKSGDEMTSLKDYVTRMKEGQK 261
Query: 97 LVIASYVLGEIPSIKDRITILRQLWDQTQDVLVLVEPGTPH---------GSSIIAQMRS 147
+ Y+ GE + L +L + +VL +V+ + G +++ +
Sbjct: 262 DIY--YITGESKKAVENSPFLERLKKKGYEVLYMVDAIDEYAVGQLKEYDGKKLVSATKE 319
Query: 148 YILW---MEERKHRKSSKKNN-----EVCKDLITEKAGAFVVAPCPHDGTCPLVKSGKYC 199
+ EE K +K KK + ++ KD++ +K VV+ D C LV +G+Y
Sbjct: 320 GLKLDDDSEEEKKKKEEKKKSFENLCKIIKDILGDKVEKVVVSDRIVDSPCCLV-TGEY- 377
Query: 200 HFVQRLERTSSQRAYKVS 217
+ +ER +A + S
Sbjct: 378 GWTANMERIMKAQALRDS 395
>ECE2_BOVIN (Q10711) Endothelin-converting enzyme 2 (EC 3.4.24.71)
(ECE-2)
Length = 787
Score = 31.2 bits (69), Expect = 2.0
Identities = 23/116 (19%), Positives = 48/116 (40%), Gaps = 1/116 (0%)
Query: 81 IQALSKDISKSERGHDLVIASYVL-GEIPSIKDRITILRQLWDQTQDVLVLVEPGTPHGS 139
+++L + +S E + ++ +P + R LWDQ Q +L + T S
Sbjct: 129 LESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNNSNSLWDQNQAILKHLLENTTFNS 188
Query: 140 SIIAQMRSYILWMEERKHRKSSKKNNEVCKDLITEKAGAFVVAPCPHDGTCPLVKS 195
S A+ ++ ++ + + + +DLI + G V P D ++K+
Sbjct: 189 SSEAERKTQRFYLSCLQVERIEELGAHALRDLIDKIGGWNVTGPWDQDNFMEVLKA 244
>CGB1_MOUSE (P24860) G2/mitotic-specific cyclin B1
Length = 430
Score = 31.2 bits (69), Expect = 2.0
Identities = 18/54 (33%), Positives = 27/54 (49%), Gaps = 7/54 (12%)
Query: 1 MGEVGVRMLVEVSRRIDGLSPTKVLDFGAGTVSAFWALQEVWPKSLEKVNIIEP 54
+G++G ++ E+ R+ K L G GTV A PK +EKV + EP
Sbjct: 45 LGDIGNKVSEELQARVPLKREAKTLGTGKGTVKAL-------PKPVEKVPVCEP 91
>NOT4_YEAST (P34909) General negative regulator of transcription
subunit 4
Length = 587
Score = 30.4 bits (67), Expect = 3.3
Identities = 18/55 (32%), Positives = 28/55 (50%), Gaps = 9/55 (16%)
Query: 153 EERKHRKSSKKNNE------VCKDLITEKAGAFVVA---PCPHDGTCPLVKSGKY 198
+ERKHR+ +K NE + + +K +VV P P++ P +KS KY
Sbjct: 108 KERKHREKERKENEYTNRKHLSGTRVIQKNLVYVVGINPPVPYEEVAPTLKSEKY 162
>HEMZ_PSESM (Q888A2) Ferrochelatase (EC 4.99.1.1) (Protoheme
ferro-lyase) (Heme synthetase)
Length = 340
Score = 30.0 bits (66), Expect = 4.3
Identities = 22/76 (28%), Positives = 31/76 (39%)
Query: 109 SIKDRITILRQLWDQTQDVLVLVEPGTPHGSSIIAQMRSYILWMEERKHRKSSKKNNEVC 168
S+K + ++L+ +DQ + V LVE PH + + ER K
Sbjct: 148 SLKMQFSVLQPFYDQPEYVSALVESVRPHLEQPYDHLLLSFHGLPERHLHKRDPTGKHCL 207
Query: 169 KDLITEKAGAFVVAPC 184
KD A A VVA C
Sbjct: 208 KDDCCMTAPAQVVATC 223
>LAP1_HUMAN (Q96NW7) LAP1 protein (Densin-180)
Length = 1537
Score = 29.6 bits (65), Expect = 5.7
Identities = 17/55 (30%), Positives = 29/55 (51%), Gaps = 2/55 (3%)
Query: 81 IQALSKDISKSERGHDLVIASYVLGEIPSIKDRITILRQLWDQTQDVLVLVEPGT 135
++ L K + K + L + + GE+P + D+I LR+LW + VL PG+
Sbjct: 173 LKTLPKSMHKLAQLERLDLGNNEFGELPEVLDQIQNLRELWMDNNALQVL--PGS 225
>HS83_IPONI (P51819) Heat shock protein 83
Length = 703
Score = 29.6 bits (65), Expect = 5.7
Identities = 46/198 (23%), Positives = 86/198 (43%), Gaps = 33/198 (16%)
Query: 40 EVWPKSLEKVNIIEPSQSMQRAGQRLIQGLKDLPLIHSY---DSIQALSKDISKSERGHD 96
E + K+L K+ I E SQ+ + L DL +S D + +L +++ + G
Sbjct: 415 EAFSKNL-KLGIHEDSQNRAK--------LADLLRYYSTKSGDELTSLKDYVTRMKEGQK 465
Query: 97 LVIASYVLGEIPSIKDRITILRQLWDQTQDVLVLVEPGTPH---------GSSIIAQMRS 147
+ Y+ GE + L +L + +VL +V+ + G +++ +
Sbjct: 466 DIY--YITGESKKAVENSPFLERLKKKGYEVLFMVDAIDEYAVGQLKEYDGKKLVSATKE 523
Query: 148 YILWM----EERKHRKSSKKNNE----VCKDLITEKAGAFVVAPCPHDGTCPLVKSGKYC 199
+ EE+K R+ KK+ E + KD++ +K VV+ D C LV +G+Y
Sbjct: 524 GLKLEDDDEEEKKKREEKKKSFENLCKIIKDILGDKVEKVVVSDRIVDSPCCLV-TGEY- 581
Query: 200 HFVQRLERTSSQRAYKVS 217
+ +ER +A + S
Sbjct: 582 GWTANMERIMKAQALRDS 599
>CPPM_BACHD (Q9Z9T7) Putative carboxyvinyl-carboxyphosphonate
phosphorylmutase (EC 2.7.8.23)
(Carboxyphosphonoenolpyruvate phosphonomutase) (CPEP
phosphonomutase)
Length = 300
Score = 29.6 bits (65), Expect = 5.7
Identities = 17/51 (33%), Positives = 28/51 (54%)
Query: 44 KSLEKVNIIEPSQSMQRAGQRLIQGLKDLPLIHSYDSIQALSKDISKSERG 94
K+ E++ + + Q AG +Q K+L SYD +AL K+I+K+ G
Sbjct: 249 KAYERIFQLIKDEGTQEAGIEDMQTRKELYETISYDDFEALDKNIAKTVLG 299
>DCTD_RHILE (P10046) C4-dicarboxylate transport transcriptional
regulatory protein dctD
Length = 448
Score = 29.3 bits (64), Expect = 7.4
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 4/54 (7%)
Query: 103 VLGEIPSIKDRITILRQLWDQTQDVLVLVEPGTPHGSSIIAQMRSYILWMEERK 156
++G+ P +++ ILR + D DVLV E G+ G ++AQ+ W RK
Sbjct: 145 LIGQTPVMENLRNILRHIADTDVDVLVAGETGS--GKEVVAQILHQ--WSHRRK 194
>TP2A_MOUSE (Q01320) DNA topoisomerase II, alpha isozyme (EC
5.99.1.3)
Length = 1528
Score = 28.9 bits (63), Expect = 9.7
Identities = 25/93 (26%), Positives = 45/93 (47%), Gaps = 16/93 (17%)
Query: 63 QRLIQGLKDLPLIHSYDSIQALSKDISKSERGHDLVIASYVLGEIPSIKDRITI-LRQL- 120
+RL+ G + LP++ SY + + ++++ ++ YV+ +I D TI + +L
Sbjct: 876 RRLLDGEEPLPMLPSYKNFKGTIEELASNQ---------YVINGEVAILDSTTIEISELP 926
Query: 121 ---WDQT--QDVLVLVEPGTPHGSSIIAQMRSY 148
W QT + VL + GT S+I R Y
Sbjct: 927 IRTWTQTYKEQVLEPMLNGTEKTPSLITDYREY 959
>LEU3_BUCAP (O85064) 3-isopropylmalate dehydrogenase (EC 1.1.1.85)
(Beta-IPM dehydrogenase) (IMDH) (3-IPM-DH)
Length = 363
Score = 28.9 bits (63), Expect = 9.7
Identities = 17/65 (26%), Positives = 33/65 (50%), Gaps = 2/65 (3%)
Query: 45 SLEKVNIIEPSQSMQRAGQRLIQGLKDLPLIHSYDSIQALSKDISKSERGHDLVIASYVL 104
S++K N++E S + +++ + D+ L H Y I I K+ D+++ S +
Sbjct: 192 SIDKANVLESSVLWREIVKKVSKNYPDVHLSHLY--IDNACMQIIKNPTQFDILLCSNIF 249
Query: 105 GEIPS 109
G+I S
Sbjct: 250 GDIIS 254
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,042,276
Number of Sequences: 164201
Number of extensions: 1001159
Number of successful extensions: 2479
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 2472
Number of HSP's gapped (non-prelim): 21
length of query: 218
length of database: 59,974,054
effective HSP length: 106
effective length of query: 112
effective length of database: 42,568,748
effective search space: 4767699776
effective search space used: 4767699776
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)
Medicago: description of AC149197.7