BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149197.6 + phase: 0 /pseudo
(174 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
KTN1_CHICK (Q90631) Kinectin 34 0.15
CO4_HUMAN (P01028) Complement C4 precursor [Contains: C4a anaphy... 33 0.25
APU_THETU (P38536) Amylopullulanase precursor (Alpha-amylase/pul... 33 0.43
DPOL_THEHY (Q9HH05) DNA polymerase (EC 2.7.7.7) [Contains: Endon... 32 0.73
DP27_CAEEL (P48996) Chromosome condensation protein dpy-27 (Dump... 32 0.73
RELN_RAT (P58751) Reelin precursor (EC 3.4.21.-) 32 0.96
RELN_MOUSE (Q60841) Reelin precursor (EC 3.4.21.-) (Reeler protein) 32 0.96
PABP_YEAST (P04147) Polyadenylate-binding protein, cytoplasmic a... 32 0.96
SMC1_XENLA (O93308) Structural maintenance of chromosome 1 protein 31 1.3
ERC1_HUMAN (Q8IUD2) ERC protein 1 (ELKS protein) 31 1.3
RELN_HUMAN (P78509) Reelin precursor (EC 3.4.21.-) 31 1.6
AMPN_PLUXY (P91887) Aminopeptidase N precursor (EC 3.4.11.2) (Mi... 31 1.6
EF2_CRYPV (Q23716) Elongation factor 2 (EF-2) 30 2.1
RELN_CHICK (O93574) Reelin (EC 3.4.21.-) (Fragment) 30 2.8
5NTC_HUMAN (P49902) Cytosolic purine 5'-nucleotidase (EC 3.1.3.5... 30 2.8
SM1A_HUMAN (Q14683) Structural maintenance of chromosome 1-like ... 30 3.6
SM1A_BOVIN (O97593) Structural maintenance of chromosome 1-like ... 30 3.6
RGYR_THEMA (O51934) Reverse gyrase [Includes: Helicase (EC 3.6.1... 30 3.6
PXF_HUMAN (P40855) Peroxisomal farnesylated protein (33 kDa hous... 30 3.6
PXF_CRIGR (Q60415) Peroxisomal farnesylated protein 30 3.6
>KTN1_CHICK (Q90631) Kinectin
Length = 1364
Score = 34.3 bits (77), Expect = 0.15
Identities = 31/114 (27%), Positives = 51/114 (44%), Gaps = 20/114 (17%)
Query: 4 ESFPTHLPVFEGKNYDQWIVKMKVICRFQDVLEVVNDSVPALAS---------NATDMQ* 54
ESF + + +N Q + + +++L+VV +AS NA + Q
Sbjct: 977 ESFKVQIQELKQENCKQ----ASLAVQSEELLQVVAGKEKEIASLQNELACQRNAFEQQR 1032
Query: 55 TANRDAKKKDGKAMF-------FMHQSVSNEIFEKIVHYESAKEETWEALEKLY 101
N D ++K+ KAM + V+ EK H E+A+ ET E L+KL+
Sbjct: 1033 KKNNDLREKNWKAMEALASTEKLLQDKVNKTAKEKQQHVEAAEVETRELLQKLF 1086
>CO4_HUMAN (P01028) Complement C4 precursor [Contains: C4a
anaphylatoxin; C4b]
Length = 1744
Score = 33.5 bits (75), Expect = 0.25
Identities = 26/74 (35%), Positives = 33/74 (44%), Gaps = 2/74 (2%)
Query: 63 KDGKAMFFMHQSVSNEIFEKIVHYESAKEETWEALEKLYSGDGKLKKVRLQVLRRQYEVL 122
+DGK FF ++ H +K E +ALEKL G L+ +RL V E
Sbjct: 289 EDGKKTFFRGLESQTKLVNGQSHISLSKAEFQDALEKLNMGITDLQGLRLYVAAAIIESP 348
Query: 123 --TMEEEETISQYF 134
MEE E S YF
Sbjct: 349 GGEMEEAELTSWYF 362
>APU_THETU (P38536) Amylopullulanase precursor
(Alpha-amylase/pullulanase) (Pullulanase type II)
[Includes: Alpha-amylase (EC 3.2.1.1) (1,4-alpha-D-glucan
glucanohydrolase); Pullulanase (EC 3.2.1.41)
(1,4-alpha-D-glucan glucanohydrolase) (Alpha-dextrin en
Length = 1861
Score = 32.7 bits (73), Expect = 0.43
Identities = 31/146 (21%), Positives = 58/146 (39%), Gaps = 12/146 (8%)
Query: 8 THLPVFEGKNYDQWIVKMKVICRFQDVLEVVNDSVPALASNATDMQ*TANRDAKKKDGKA 67
TH + YD+ +K +DV+EV+ + TD Q N+ + + A
Sbjct: 1668 THFSQYAAFEYDKTFNDIKDNWA-KDVIEVLASR--HIVEGMTDTQYEPNKTVTRAEFTA 1724
Query: 68 MFFMHQSVSNEIFEKIVHYESAKEETW--EALEKLYS-----GDGKLKKVRLQVLRRQYE 120
M ++ +E + + K W A+E Y GDGK + + R +
Sbjct: 1725 MILRLLNIKDETYSG--EFSDVKSGDWYANAIEAAYKAGIIEGDGKNARPNDSITREEMT 1782
Query: 121 VLTMEEEETISQYFDKFVNLSNQSQE 146
+ M E ++QY ++ + + S +
Sbjct: 1783 AIAMRAYEMLTQYKEENIGATTFSDD 1808
>DPOL_THEHY (Q9HH05) DNA polymerase (EC 2.7.7.7) [Contains:
Endonuclease PI-ThyII (EC 3.1.-.-) (Thy pol-1 intein);
Endonuclease PI-ThyI (EC 3.1.-.-) (Thy pol-2 intein)]
(Fragment)
Length = 1668
Score = 32.0 bits (71), Expect = 0.73
Identities = 24/60 (40%), Positives = 35/60 (58%), Gaps = 5/60 (8%)
Query: 73 QSVSNEIFEKIVHYESAKEETWEALEKLYSGDGKLKKVRLQVLRRQYE----VLTMEEEE 128
+SVS E F ++V E E + +E L +GD L KV L+V +R YE L++EE+E
Sbjct: 924 RSVSYEKFRELVKSEKLDGEKAKRIEWLLNGDVVLDKV-LEVKKRPYEGYVYDLSVEEDE 982
>DP27_CAEEL (P48996) Chromosome condensation protein dpy-27
(Dumpty-27 protein)
Length = 1469
Score = 32.0 bits (71), Expect = 0.73
Identities = 35/151 (23%), Positives = 61/151 (40%), Gaps = 24/151 (15%)
Query: 9 HLPVFEGKNYDQWIVKMKVICRFQDVLEVVNDSVPALASNATDMQ*TANRDAKKKDGKAM 68
+L F NY + I +CR+ E + D+ +L + +++ + + KD
Sbjct: 317 YLNTFNNLNYLRGIRVKHNLCRYA---ETMRDAKMSLVTRTGELEENKDIMLEAKDEVRK 373
Query: 69 FFMHQ----SVSNEIFEKIVHYESAKEETWEA--------LEKLYSGDGKLKKVRLQVLR 116
H+ S+ E+ K + ++S K + W A L+ GKL K R + R
Sbjct: 374 KETHERSLNSIVTELENKRIDWQSKKND-WHARDAKRKQGLKSCTQDLGKLMKERDEARR 432
Query: 117 RQYEVLTMEEEETISQYFDKFVNLSNQSQEW 147
++E+ T E IS+ N EW
Sbjct: 433 EKFEIETAPENARISK--------QNMQLEW 455
>RELN_RAT (P58751) Reelin precursor (EC 3.4.21.-)
Length = 3462
Score = 31.6 bits (70), Expect = 0.96
Identities = 15/35 (42%), Positives = 23/35 (64%), Gaps = 2/35 (5%)
Query: 11 PVFEGKNYDQWIVKMKVIC--RFQDVLEVVNDSVP 43
PVF G++YDQW V +I + + V+ VVN ++P
Sbjct: 1204 PVFSGEDYDQWAVDDIIILSEKQKQVIPVVNPTLP 1238
>RELN_MOUSE (Q60841) Reelin precursor (EC 3.4.21.-) (Reeler protein)
Length = 3461
Score = 31.6 bits (70), Expect = 0.96
Identities = 15/35 (42%), Positives = 23/35 (64%), Gaps = 2/35 (5%)
Query: 11 PVFEGKNYDQWIVKMKVIC--RFQDVLEVVNDSVP 43
PVF G++YDQW V +I + + V+ VVN ++P
Sbjct: 1203 PVFSGEDYDQWAVDDIIILSEKQKQVIPVVNPTLP 1237
>PABP_YEAST (P04147) Polyadenylate-binding protein, cytoplasmic and
nuclear (Poly(A)-binding protein) (PABP) (ARS consensus
binding protein ACBP-67) (Polyadenylate tail-binding
protein)
Length = 576
Score = 31.6 bits (70), Expect = 0.96
Identities = 23/66 (34%), Positives = 32/66 (47%), Gaps = 2/66 (3%)
Query: 61 KKKDGKAMFFMHQSVSNEIFEKIVHYESAKEETWEALEKLYSGDGKLKKVRLQVLRRQYE 120
K DGK F V+ E E V A ++ EKLY G + K R+ VL++QYE
Sbjct: 251 KDADGKLKGFGF--VNYEKHEDAVKAVEALNDSELNGEKLYVGRAQKKNERMHVLKKQYE 308
Query: 121 VLTMEE 126
+E+
Sbjct: 309 AYRLEK 314
>SMC1_XENLA (O93308) Structural maintenance of chromosome 1 protein
Length = 1232
Score = 31.2 bits (69), Expect = 1.3
Identities = 27/99 (27%), Positives = 47/99 (47%), Gaps = 12/99 (12%)
Query: 58 RDAKKKDGKAMFFMHQSVSNEIFEK-------IVHYESAKEETWEALEKLYSGDGKLKKV 110
+D KK+ GK M Q++ EI EK + Y AKE ++K + L+
Sbjct: 264 KDKKKELGK-MMREQQAIEKEIKEKDAELNQKLPQYIKAKENPSHKIKKFRAAKKSLQNA 322
Query: 111 RLQVLRRQYEVLTMEEE----ETISQYFDKFVNLSNQSQ 145
+ Q +R+ ++ +E+E E Q F++ + +QSQ
Sbjct: 323 QKQYKKRKADMDELEKEMLSVEKARQEFEERMEEESQSQ 361
>ERC1_HUMAN (Q8IUD2) ERC protein 1 (ELKS protein)
Length = 1116
Score = 31.2 bits (69), Expect = 1.3
Identities = 28/119 (23%), Positives = 58/119 (48%), Gaps = 7/119 (5%)
Query: 20 QWIVKMK--VICRFQDVLEVVNDSVPALASNATDMQ*TANRDAKKKDGKAMFFMHQSVSN 77
Q +++MK I + L + + + L SN T R+ + K + ++ H
Sbjct: 381 QTVIEMKDSKISSMERGLRDLEEEIQMLKSNGALS--TEEREEEMKQME-VYRSHSKFMK 437
Query: 78 EIFEKIVHYESAKEETWEALEKLYSG-DGKLKKVRLQVLRRQYEVLTMEEE-ETISQYF 134
E++ S+KE WE L+K +G ++ +V+ ++ R+ E+L ++ + ET++ F
Sbjct: 438 NKVEQLKEELSSKEAQWEELKKKAAGLQAEIGQVKQELSRKDTELLALQTKLETLTNQF 496
>RELN_HUMAN (P78509) Reelin precursor (EC 3.4.21.-)
Length = 3460
Score = 30.8 bits (68), Expect = 1.6
Identities = 13/35 (37%), Positives = 23/35 (65%), Gaps = 2/35 (5%)
Query: 11 PVFEGKNYDQWIVKMKVIC--RFQDVLEVVNDSVP 43
PVF G++YDQW V +I + + ++ V+N ++P
Sbjct: 1202 PVFSGEDYDQWAVDDIIILSEKQKQIIPVINPTLP 1236
>AMPN_PLUXY (P91887) Aminopeptidase N precursor (EC 3.4.11.2)
(Microsomal aminopeptidase) (APN1)
Length = 946
Score = 30.8 bits (68), Expect = 1.6
Identities = 22/79 (27%), Positives = 38/79 (47%), Gaps = 2/79 (2%)
Query: 4 ESFPTHLPVFEGKNYDQWIVKMKVICRFQDVLEVVNDSVPALASNATDMQ*TANRDAKKK 63
ESF T L + E + DQ+++ M+ + + VL V + + + + T N A +K
Sbjct: 374 ESFATDLVLPEWRMMDQFVINMQNVFQSDAVLSV--NPITFEVRTPSQILGTFNSVAYQK 431
Query: 64 DGKAMFFMHQSVSNEIFEK 82
G + M ++ EIF K
Sbjct: 432 SGSVIRMMQHFLTPEIFRK 450
>EF2_CRYPV (Q23716) Elongation factor 2 (EF-2)
Length = 832
Score = 30.4 bits (67), Expect = 2.1
Identities = 20/57 (35%), Positives = 34/57 (59%), Gaps = 4/57 (7%)
Query: 89 AKEETWEALEKLYSGDGKLK-KVRLQVLRRQYEVLTMEEEETISQYFDKFVNLSNQS 144
+KEET E + +G G+L ++ LQ L+++Y + + + I Y + VNLSNQ+
Sbjct: 511 SKEETGE---HIIAGCGELHVEICLQDLQQEYAQIEIVASDPIVSYRETVVNLSNQT 564
>RELN_CHICK (O93574) Reelin (EC 3.4.21.-) (Fragment)
Length = 3209
Score = 30.0 bits (66), Expect = 2.8
Identities = 13/35 (37%), Positives = 22/35 (62%), Gaps = 2/35 (5%)
Query: 11 PVFEGKNYDQWIVKMKVIC--RFQDVLEVVNDSVP 43
PVF G+ YDQW + +I + + ++ VVN ++P
Sbjct: 950 PVFSGEGYDQWAIDDIIILSEKQKHIIPVVNPTLP 984
>5NTC_HUMAN (P49902) Cytosolic purine 5'-nucleotidase (EC 3.1.3.5)
(5'-nucleotidase cytosolic II)
Length = 561
Score = 30.0 bits (66), Expect = 2.8
Identities = 20/48 (41%), Positives = 30/48 (61%), Gaps = 4/48 (8%)
Query: 63 KDGKAMFFMHQSVSNEIFEKI--VHYE-SAKEETWEALEKLYSGDGKL 107
KDG +F ++S+ ++ + + VHY+ S KE+T E LEK DGKL
Sbjct: 186 KDGD-LFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKL 232
>SM1A_HUMAN (Q14683) Structural maintenance of chromosome 1-like 1
protein (SMC1alpha protein) (SB1.8/DXS423E protein)
(Sb1.8)
Length = 1233
Score = 29.6 bits (65), Expect = 3.6
Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 10/98 (10%)
Query: 58 RDAKKKDGKAMFFMHQ------SVSNEIFEKIVHYESAKEETWEALEKLYSGDGKLKKVR 111
++ KK+ GK M Q +E+ +K Y AKE T ++KL + L+ +
Sbjct: 264 KEKKKELGKMMREQQQIEKEIKEKDSELNQKRPQYIKAKENTSHKIKKLEAAKKSLQNAQ 323
Query: 112 LQVLRRQYEVLTMEEE----ETISQYFDKFVNLSNQSQ 145
+R+ ++ +E+E E Q F++ + +QSQ
Sbjct: 324 KHYKKRKGDMDELEKEMLSVEKARQEFEERMEEESQSQ 361
>SM1A_BOVIN (O97593) Structural maintenance of chromosome 1-like 1
protein (SMC-protein)
Length = 1233
Score = 29.6 bits (65), Expect = 3.6
Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 10/98 (10%)
Query: 58 RDAKKKDGKAMFFMHQ------SVSNEIFEKIVHYESAKEETWEALEKLYSGDGKLKKVR 111
++ KK+ GK M Q +E+ +K Y AKE T ++KL + L+ +
Sbjct: 264 KEKKKELGKMMREQQQIEKEIKEKDSELNQKRPQYIKAKENTSHKIKKLEAAKKSLQNAQ 323
Query: 112 LQVLRRQYEVLTMEEE----ETISQYFDKFVNLSNQSQ 145
+R+ ++ +E+E E Q F++ + +QSQ
Sbjct: 324 KHYKKRKGDMDELEKEMLSVEKARQEFEERMEEESQSQ 361
>RGYR_THEMA (O51934) Reverse gyrase [Includes: Helicase (EC
3.6.1.-); Topoisomerase (EC 5.99.1.3)]
Length = 1104
Score = 29.6 bits (65), Expect = 3.6
Identities = 20/91 (21%), Positives = 42/91 (45%), Gaps = 8/91 (8%)
Query: 31 FQDVLEVVNDSVPALASNATDMQ*TANRDAKK-------KDGKAMFFMHQSVSNEIFEKI 83
F+D+L + ++A N T ++ ++ K +DG +F + E++E +
Sbjct: 270 FRDLLNFTVGRLVSVARNITHVRISSRSKEKLVELLEIFRDGILIFAQTEEEGKELYEYL 329
Query: 84 VHYESAKEETWEALEKLYSGDGKLKKVRLQV 114
++ ETW EK + D K+ K+ + +
Sbjct: 330 KRFKFNVGETWSEFEKNFE-DFKVGKINILI 359
>PXF_HUMAN (P40855) Peroxisomal farnesylated protein (33 kDa
housekeeping protein) (Peroxin 19)
Length = 299
Score = 29.6 bits (65), Expect = 3.6
Identities = 32/138 (23%), Positives = 56/138 (40%), Gaps = 23/138 (16%)
Query: 32 QDVLEVVNDSVPALASNATDMQ*TANRD---AKKKDGKAM---------FFMHQSVSNEI 79
Q+ + +++ LA NATD+Q ++ + K +G M + QS+ +
Sbjct: 123 QEFTSCLKETLSGLAKNATDLQNSSMSEEELTKAMEGLGMDEGDGEGNILPIMQSIMQNL 182
Query: 80 FEKIVHYESAKEETWEALEKLYSGDGKLKKVRLQVLRRQYEVL-----------TMEEEE 128
K V Y S KE T + E L S L + + + Q+ V+ + E
Sbjct: 183 LSKDVLYPSLKEITEKYPEWLQSHRESLPPEQFEKYQEQHSVMCKICEQFEAETPTDSET 242
Query: 129 TISQYFDKFVNLSNQSQE 146
T F+ ++L Q Q+
Sbjct: 243 TQKARFEMVLDLMQQLQD 260
>PXF_CRIGR (Q60415) Peroxisomal farnesylated protein
Length = 299
Score = 29.6 bits (65), Expect = 3.6
Identities = 32/139 (23%), Positives = 54/139 (38%), Gaps = 25/139 (17%)
Query: 32 QDVLEVVNDSVPALASNATDMQ*TA-------------NRDAKKKDGKAMFFMHQSVSNE 78
Q+ + +++ LA NATD+Q + D DG + M QS+
Sbjct: 123 QEFTSCLKETLSGLAKNATDLQNSGMSEEELTKAMEGLGMDDGDGDGNILPIM-QSIMQN 181
Query: 79 IFEKIVHYESAKEETWEALEKLYSGDGKLKKVRLQVLRRQYEVL-----------TMEEE 127
+ K V Y S KE T + E L S + + + + Q+ V+ + E
Sbjct: 182 LLSKDVLYPSLKEITEKYPEWLQSHQESIPPEQFEKYQEQHSVMGKICEQFEAETPTDSE 241
Query: 128 ETISQYFDKFVNLSNQSQE 146
T F+ ++L Q Q+
Sbjct: 242 ATHRARFEAVLDLMQQLQD 260
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.332 0.140 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,093,941
Number of Sequences: 164201
Number of extensions: 660227
Number of successful extensions: 3411
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 30
Number of HSP's that attempted gapping in prelim test: 3401
Number of HSP's gapped (non-prelim): 37
length of query: 174
length of database: 59,974,054
effective HSP length: 103
effective length of query: 71
effective length of database: 43,061,351
effective search space: 3057355921
effective search space used: 3057355921
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 62 (28.5 bits)
Medicago: description of AC149197.6