BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149050.9 - phase: 0 /pseudo
(157 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PMP2_MOUSE (P42925) Peroxisomal membrane protein 2 (22 kDa perox... 96 3e-20
PMP2_RAT (Q07066) Peroxisomal membrane protein 2 (22 kDa peroxis... 94 1e-19
PMP2_HUMAN (Q9NR77) Peroxisomal membrane protein 2 (22 kDa perox... 92 5e-19
MPV1_MOUSE (P19258) Mpv17 protein 85 8e-17
MPV1_HUMAN (P39210) Mpv17 protein 82 6e-16
YD1E_SCHPO (Q10244) Hypothetical protein C4G9.14 in chromosome I 52 7e-07
MAL2_HUMAN (Q969L2) MAL2 protein 40 0.003
PIGQ_HUMAN (Q9BRB3) Phosphatidylinositol N-acetylglucosaminyltra... 34 0.15
YF46_ARCFU (O28726) Hypothetical UPF0003 protein AF1546 31 1.3
ALA4_ARATH (Q9LNQ4) Potential phospholipid-transporting ATPase 4... 30 2.2
FVR1_HUMAN (Q9Y5Y0) Feline leukemia virus subgroup C receptor-re... 30 2.9
YPNP_BACSU (P54181) Hypothetical protein ypnP 29 3.8
SPP2_MOUSE (Q810K3) Sphingosine-1-phosphate phosphatase 2 (EC 3.... 29 4.9
FVR1_MUSDU (Q9ES43) Feline leukemia virus subgroup C receptor-re... 29 4.9
COX3_SCHPO (P14575) Cytochrome c oxidase polypeptide III (EC 1.9... 29 4.9
COX3_PODAN (Q02654) Cytochrome c oxidase polypeptide III (EC 1.9... 29 4.9
CNG9_ARATH (Q9M0A4) Putative cyclic nucleotide-gated ion channel... 29 4.9
TIP_PLAYO (Q7RRM4) T-cell immunomodulatory protein homolog precu... 28 6.4
OLF9_RAT (P23272) Olfactory receptor-like protein I9 28 8.4
O1I1_HUMAN (O60431) Olfactory receptor 1I1 (Olfactory receptor 1... 28 8.4
>PMP2_MOUSE (P42925) Peroxisomal membrane protein 2 (22 kDa
peroxisomal membrane protein)
Length = 193
Score = 95.9 bits (237), Expect = 3e-20
Identities = 57/153 (37%), Positives = 91/153 (59%), Gaps = 7/153 (4%)
Query: 6 RYMALLAKYPVPVKALTSAILNLIGDLICQLVIDKVQTPD---LKRTFLFSFL--GLVLV 60
+Y+ LL YPV KA++S IL+ +G+L+ Q I+K Q D L+ + L +L GL +
Sbjct: 23 QYLLLLKLYPVLTKAVSSGILSALGNLLAQ-TIEKKQRKDSRLLEVSGLLRYLVYGLFVT 81
Query: 61 GPTLHFWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIFLGVFLSSLVTLEGRP-SQAVPK 119
GP H+ YL++ V ++ RL+LD+ F+P FL +F + LEG+ S V K
Sbjct: 82 GPLSHYLYLFMEYSVPPEVPWASVKRLLLDRLFFAPTFLLLFFFVMNLLEGKNVSVFVAK 141
Query: 120 LKQEWFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
++ ++ A+ NW++W P QF+N +VP QF+V
Sbjct: 142 MRSGFWPALQMNWRMWTPLQFININYVPLQFRV 174
>PMP2_RAT (Q07066) Peroxisomal membrane protein 2 (22 kDa
peroxisomal membrane protein)
Length = 193
Score = 94.4 bits (233), Expect = 1e-19
Identities = 56/153 (36%), Positives = 90/153 (58%), Gaps = 7/153 (4%)
Query: 6 RYMALLAKYPVPVKALTSAILNLIGDLICQLVIDKVQTPD---LKRTFLFSFL--GLVLV 60
+Y+ L YPV KA++S IL+ +G+L+ Q+ I+K Q D L+ + L +L GL +
Sbjct: 23 QYLLFLKFYPVVTKAVSSGILSALGNLLAQM-IEKKQKKDSRSLEVSGLLRYLVYGLFVT 81
Query: 61 GPTLHFWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIFLGVFLSSLVTLEGRP-SQAVPK 119
GP H+ YL++ V + RL+LD+ F+P FL +F + LEG+ S V K
Sbjct: 82 GPLSHYLYLFMEYWVPPEVPWARVKRLLLDRLFFAPTFLLLFFFVMNLLEGKNISVFVAK 141
Query: 120 LKQEWFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
++ ++ A+ NW++W P QF+N +VP QF+V
Sbjct: 142 MRSGFWPALQMNWRMWTPLQFININYVPLQFRV 174
>PMP2_HUMAN (Q9NR77) Peroxisomal membrane protein 2 (22 kDa
peroxisomal membrane protein)
Length = 194
Score = 92.0 bits (227), Expect = 5e-19
Identities = 48/153 (31%), Positives = 84/153 (54%), Gaps = 6/153 (3%)
Query: 6 RYMALLAKYPVPVKALTSAILNLIGDLICQLVIDK-----VQTPDLKRTFLFSFLGLVLV 60
+Y+ L YPV KA TS IL+ +G+ + Q++ K ++ D+ ++ G
Sbjct: 23 QYLLFLRLYPVLTKAATSGILSALGNFLAQMIEKKRKKENSRSLDVGGPLRYAVYGFFFT 82
Query: 61 GPTLHFWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIFLGVFLSSLVTLEGRPSQA-VPK 119
GP HF+Y ++ + + RL+LD+ VF+P FL +F + LEG+ + A K
Sbjct: 83 GPLSHFFYFFMEHWIPPEVPLAGLRRLLLDRLVFAPAFLMLFFLIMNFLEGKDASAFAAK 142
Query: 120 LKQEWFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
++ ++ A+ NW++W P QF+N +VP +F+V
Sbjct: 143 MRGGFWPALRMNWRVWTPLQFININYVPLKFRV 175
>MPV1_MOUSE (P19258) Mpv17 protein
Length = 176
Score = 84.7 bits (208), Expect = 8e-17
Identities = 55/157 (35%), Positives = 85/157 (54%), Gaps = 7/157 (4%)
Query: 1 MTLYYRYMALLAKYPVPVKALTSAILNLIGDLICQLVIDK--VQTPDLKRTFLFSFLGLV 58
M L+ Y LA +P V+ LT+ L +GD+I Q ++++ +Q RT LG
Sbjct: 1 MALWRAYQRALAAHPWKVQVLTAGSLMGVGDMISQQLVERRGLQQHQAGRTLTMVSLGCG 60
Query: 59 LVGPTLHFWYLYLSQLVTLPGTSG--AILRLVLDQFVFSPIFLGVFLSSLVTLEGRPSQ- 115
VGP + WY L L+ PGT+ A+ +++LDQ F+P FLG FL + L G +Q
Sbjct: 61 FVGPVVGGWYKVLDHLI--PGTTKVHALKKMLLDQGGFAPCFLGCFLPLVGILNGMSAQD 118
Query: 116 AVPKLKQEWFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
KLK+++ A++ N+ LW Q NF VP +++
Sbjct: 119 NWAKLKRDYPDALITNYYLWPAVQLANFYLVPLHYRL 155
>MPV1_HUMAN (P39210) Mpv17 protein
Length = 176
Score = 81.6 bits (200), Expect = 6e-16
Identities = 53/157 (33%), Positives = 85/157 (53%), Gaps = 7/157 (4%)
Query: 1 MTLYYRYMALLAKYPVPVKALTSAILNLIGDLICQLVIDK--VQTPDLKRTFLFSFLGLV 58
M L+ Y LA +P V+ LT+ L +GD+I Q ++++ +Q RT LG
Sbjct: 1 MALWRAYQRALAAHPWKVQVLTAGSLMGLGDIISQQLVERRGLQEHQRGRTLTMVSLGCG 60
Query: 59 LVGPTLHFWYLYLSQLVTLPGTS--GAILRLVLDQFVFSPIFLGVFLSSLVTLEGRPSQ- 115
VGP + WY L + + PGT+ A+ +++LDQ F+P FLG FL + L G +Q
Sbjct: 61 FVGPVVGGWYKVLDRFI--PGTTKVDALKKMLLDQGGFAPCFLGCFLPLVGALNGLSAQD 118
Query: 116 AVPKLKQEWFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
KL++++ A++ N+ LW Q NF VP +++
Sbjct: 119 NWAKLQRDYPDALITNYYLWPAVQLANFYLVPLHYRL 155
>YD1E_SCHPO (Q10244) Hypothetical protein C4G9.14 in chromosome I
Length = 221
Score = 51.6 bits (122), Expect = 7e-07
Identities = 34/110 (30%), Positives = 56/110 (50%), Gaps = 3/110 (2%)
Query: 45 DLKRTFLFSFLGLVLVGPTLHFWYLYLSQLVTLPGTSGAI-LRLVLDQFVFSPIFLGVFL 103
D+ RT ++ GL L P W++ LS ++ AI LR+ LDQF+F+P+ + F
Sbjct: 88 DVHRTIRYAAYGLCLT-PIQFRWFVALSNVIQTENPFIAIVLRVALDQFIFAPLGIVFFF 146
Query: 104 SSLVTLEGRPSQAVPK-LKQEWFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
+ E + + + ++ ++ + AN+ LW Q NF FVP QV
Sbjct: 147 LFMGITECKSYERLKSYFRKHYWPTLKANYILWPAVQLFNFTFVPLVLQV 196
>MAL2_HUMAN (Q969L2) MAL2 protein
Length = 176
Score = 39.7 bits (91), Expect = 0.003
Identities = 24/64 (37%), Positives = 37/64 (57%), Gaps = 1/64 (1%)
Query: 44 PDLKRTFLFSFLGLVLVGPTLHFWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIFLGVFL 103
PD+ RT+ +F+ L ++ L W L S V LP G ++ + + F FS +FLG+FL
Sbjct: 29 PDILRTYSGAFVCLEILFGGL-VWILVASSNVPLPLLQGWVMFVSVTAFFFSLLFLGMFL 87
Query: 104 SSLV 107
S +V
Sbjct: 88 SGMV 91
>PIGQ_HUMAN (Q9BRB3) Phosphatidylinositol
N-acetylglucosaminyltransferase subunit Q (EC 2.4.1.198)
(Phosphatidylinositol-glycan biosynthesis, class Q
protein) (PIG-Q) (N-acetylglucosamyl transferase
component GPI1)
Length = 759
Score = 33.9 bits (76), Expect = 0.15
Identities = 32/101 (31%), Positives = 47/101 (45%), Gaps = 9/101 (8%)
Query: 49 TFLFSFLGLVLVGPTLHFWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIF-LGVFLSSLV 107
T LF+ L +L PT +YL + L L ++ L++D P++ LG+ L
Sbjct: 449 TLLFTILLFLL--PTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYSLGLRLCRPY 506
Query: 108 TLEGRPSQAVPKLKQEWFSAVLANWQLWIPFQFLNFRFVPQ 148
L +P+ P+ A L QLW+P Q L R VPQ
Sbjct: 507 RLADKPTALQPR------GAHLPPPQLWLPPQALLGRPVPQ 541
>YF46_ARCFU (O28726) Hypothetical UPF0003 protein AF1546
Length = 283
Score = 30.8 bits (68), Expect = 1.3
Identities = 26/92 (28%), Positives = 44/92 (47%), Gaps = 3/92 (3%)
Query: 16 VPVKALTSAILNLIGDLICQLVIDKVQTPDLKRTFLFSFLGLVLVG--PTLHFWYLYLSQ 73
+ V AL + I LI + + +IDK++ L+ + G+++V L L LS
Sbjct: 25 IVVMALATIIAKLITTNLRRALIDKMKRDQLELMLKVIYFGIIIVAFIAVLPALGLDLSG 84
Query: 74 LVTLPGTSGAILRLVLDQFVFSPIFLGVFLSS 105
L+ G +G +L Q V + + G+FL S
Sbjct: 85 LLVAGGITGIVLGFA-SQSVVANLVSGIFLIS 115
>ALA4_ARATH (Q9LNQ4) Potential phospholipid-transporting ATPase 4 (EC
3.6.3.1) (Aminophospholipid flippase 4)
Length = 1216
Score = 30.0 bits (66), Expect = 2.2
Identities = 20/89 (22%), Positives = 46/89 (51%), Gaps = 2/89 (2%)
Query: 23 SAILNLIGDLICQLVIDKVQTPDLKRTFLFSFLGLVLVGPTLHFWYLYLSQLVTL-PGTS 81
+A ++ +G + +I V F+++ VL+ ++ WYL+++ + P S
Sbjct: 1040 TADMDAVGTTMFTCIIWAVNVQIALTVSHFTWIQHVLIWGSIGLWYLFVALYGMMPPSLS 1099
Query: 82 GAILRLVLDQFVFSPIF-LGVFLSSLVTL 109
G I R++++ +PI+ + FL ++ T+
Sbjct: 1100 GNIYRILVEILAPAPIYWIATFLVTVTTV 1128
>FVR1_HUMAN (Q9Y5Y0) Feline leukemia virus subgroup C
receptor-related protein 1 (Feline leukemia virus
subgroup C receptor) (hFLVCR)
Length = 555
Score = 29.6 bits (65), Expect = 2.9
Identities = 16/55 (29%), Positives = 31/55 (56%), Gaps = 6/55 (10%)
Query: 21 LTSAILNLIGDLICQLVIDKVQTPDLKRT----FLFSFLGLVLVGPTLHFWYLYL 71
LT + ++G ++C L +D +T K+T ++ SF+G+V+ TL Y+ +
Sbjct: 375 LTLVVAGMVGSILCGLWLDYTKT--YKQTTLIVYILSFIGMVIFTFTLDLRYIII 427
>YPNP_BACSU (P54181) Hypothetical protein ypnP
Length = 445
Score = 29.3 bits (64), Expect = 3.8
Identities = 19/77 (24%), Positives = 38/77 (48%), Gaps = 4/77 (5%)
Query: 30 GDLICQLVIDKVQTPDLKRTFLFSFLGLVLVGPTLHFWYLYLSQLVTLPGTSGAILRL-- 87
G + +L++ ++TP+ ++L + +G F Y ++S ++ G S LR
Sbjct: 111 GFFLSELLLRLLKTPESMIPLAETYLQIQFIGILFLFGYNFISTVLRALGDSKTPLRFIA 170
Query: 88 --VLDQFVFSPIFLGVF 102
V+ V +P+F+ VF
Sbjct: 171 FAVVLNTVLAPLFISVF 187
>SPP2_MOUSE (Q810K3) Sphingosine-1-phosphate phosphatase 2 (EC
3.1.3.-) (Sphingosine-1-phosphatase 2) (SPPase2) (Spp2)
Length = 354
Score = 28.9 bits (63), Expect = 4.9
Identities = 28/102 (27%), Positives = 44/102 (42%), Gaps = 22/102 (21%)
Query: 61 GPTLHFWYLYLSQLVTLPGTSGAILR----LVLDQFV--FSPIFLGVFLSSLVTLEGRPS 114
G TL FW + QLV+ P S +++ L D V + +G+ L LV
Sbjct: 241 GVTLGFWINHFFQLVSKPTPSLPVIQNIPPLTTDMLVLGLTKFMVGIMLILLV------R 294
Query: 115 QAVPKLKQE----WFSAVLAN------WQLWIPFQFLNFRFV 146
Q V KL + WF V N ++ +P++F+ + V
Sbjct: 295 QLVQKLSLQVLFSWFKVVTRNKEARRRLEIEVPYKFVTYTSV 336
>FVR1_MUSDU (Q9ES43) Feline leukemia virus subgroup C
receptor-related protein 1
Length = 560
Score = 28.9 bits (63), Expect = 4.9
Identities = 15/53 (28%), Positives = 31/53 (58%), Gaps = 6/53 (11%)
Query: 21 LTSAILNLIGDLICQLVIDKVQTPDLKRT----FLFSFLGLVLVGPTLHFWYL 69
LT + ++G ++C L +D +T K+T ++ SF+G+++ TL+ Y+
Sbjct: 380 LTLVVAGMVGSILCGLWLDYTKT--YKQTTLIVYVLSFIGMLIFTFTLNLGYI 430
>COX3_SCHPO (P14575) Cytochrome c oxidase polypeptide III (EC
1.9.3.1)
Length = 269
Score = 28.9 bits (63), Expect = 4.9
Identities = 21/67 (31%), Positives = 32/67 (47%), Gaps = 13/67 (19%)
Query: 50 FLFSFLGLVLVGPTLHFWYLYLSQLVTLPGTS----------GAILRLVLDQFVFSPIFL 99
F SFLGL+ T++ W+ +S + G G +L L+ + F+F+ IF
Sbjct: 49 FGISFLGLLA---TMYLWFRDMSTEANIHGAHTKAVTKGLKIGFMLFLISETFLFASIFW 105
Query: 100 GVFLSSL 106
F SSL
Sbjct: 106 AFFHSSL 112
>COX3_PODAN (Q02654) Cytochrome c oxidase polypeptide III (EC
1.9.3.1)
Length = 269
Score = 28.9 bits (63), Expect = 4.9
Identities = 23/84 (27%), Positives = 34/84 (40%), Gaps = 10/84 (11%)
Query: 45 DLKRTFLFSFLGLVLVGPTLHFWYLYLSQLVTLPG----------TSGAILRLVLDQFVF 94
+ ++ F+ L+LV ++ FW+ + TL G G IL +V + F
Sbjct: 43 NFNNSYYLFFISLILVISSMAFWFRDIISEATLLGDHTLAVQKGLNLGVILFIVSEALFF 102
Query: 95 SPIFLGVFLSSLVTLEGRPSQAVP 118
IF F SSL SQ P
Sbjct: 103 LAIFWAFFHSSLTPTVELGSQWPP 126
>CNG9_ARATH (Q9M0A4) Putative cyclic nucleotide-gated ion channel 9
(Cyclic nucleotide- and calmodulin-regulated ion channel
9)
Length = 733
Score = 28.9 bits (63), Expect = 4.9
Identities = 22/71 (30%), Positives = 40/71 (55%), Gaps = 6/71 (8%)
Query: 35 QLVIDKVQTPD--LKRTFLFSFLGLVLVGPTLHFW-YLYLSQLVTLPGTSGAILRLVLDQ 91
+LVID Q L++ F+ FL VL P + W +LY+S+ ++ T A+ ++L Q
Sbjct: 190 ELVIDPAQIAKRYLQQYFIIDFLS-VLPLPQIVVWRFLYISKGASVLATKRALRSIILVQ 248
Query: 92 FVFSPIFLGVF 102
++ P F+ ++
Sbjct: 249 YI--PRFIRLY 257
>TIP_PLAYO (Q7RRM4) T-cell immunomodulatory protein homolog
precursor
Length = 701
Score = 28.5 bits (62), Expect = 6.4
Identities = 20/64 (31%), Positives = 32/64 (49%), Gaps = 3/64 (4%)
Query: 22 TSAILNLIGDLICQLVIDKVQTPDLKRTFLFSFLGLVLVGPTLHFWYLYLSQLVTLPGTS 81
+SAI++L GD LV + + KR FL ++ ++ G +L Y+ + LP S
Sbjct: 259 SSAIVDLNGDCKADLVFTVINKKNTKRIFLEIWINKIVDGKSL---YVKADEDYMLPANS 315
Query: 82 GAIL 85
IL
Sbjct: 316 MQIL 319
>OLF9_RAT (P23272) Olfactory receptor-like protein I9
Length = 314
Score = 28.1 bits (61), Expect = 8.4
Identities = 28/80 (35%), Positives = 41/80 (51%), Gaps = 5/80 (6%)
Query: 52 FSFLGLVLVGPTLHFWY-LYLSQ-LVTLPGTSGAILRLVLDQFVFSPIFLGVFLSSLVTL 109
F LGL H +Y L+L+ L TL G I+ ++LD + +P++L FLS+L
Sbjct: 12 FFLLGLPFPPEYQHLFYALFLAMYLTTLLGNLIIIILILLDSHLHTPMYL--FLSNLSFA 69
Query: 110 E-GRPSQAVPKLKQEWFSAV 128
+ S +PKL Q S V
Sbjct: 70 DLCFSSVTMPKLLQNMQSQV 89
>O1I1_HUMAN (O60431) Olfactory receptor 1I1 (Olfactory receptor
19-20) (OR19-20)
Length = 355
Score = 28.1 bits (61), Expect = 8.4
Identities = 21/59 (35%), Positives = 32/59 (53%), Gaps = 3/59 (5%)
Query: 63 TLHFWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIFLGVFLSSLV-TLEGRPSQAVPKL 120
TL F + LVT+ G + IL ++ D + +P++ +F SLV TL S VPK+
Sbjct: 25 TLLFTMFLSTYLVTIIGNALIILAIITDSHLHTPMYFFLFNLSLVDTL--LSSTTVPKM 81
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.332 0.146 0.456
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,441,846
Number of Sequences: 164201
Number of extensions: 670557
Number of successful extensions: 1919
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1903
Number of HSP's gapped (non-prelim): 21
length of query: 157
length of database: 59,974,054
effective HSP length: 101
effective length of query: 56
effective length of database: 43,389,753
effective search space: 2429826168
effective search space used: 2429826168
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC149050.9