BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149040.5 - phase: 0
(476 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MYSN_ACACA (P05659) Myosin II heavy chain, non muscle 35 0.55
RADI_CHICK (Q9PU45) Radixin 33 1.2
PRX1_CHICK (Q91018) Homeobox prospero-like protein PROX1 (PROX 1) 33 1.2
LAP4_MOUSE (Q80U72) LAP4 protein (Scribble homolog protein) 33 1.2
TRHY_SHEEP (P22793) Trichohyalin 33 2.1
IF2_AQUAE (O67825) Translation initiation factor IF-2 33 2.1
HOK1_MOUSE (Q8BIL5) Hook homolog 1 33 2.1
HOK1_HUMAN (Q9UJC3) Hook homolog 1 (h-hook1) (hHK1) 33 2.1
Y6G2_ENCCU (Q8SV63) Hypothetical protein ECU06_1620 32 2.7
TA4B_HUMAN (Q92750) Transcription initiation factor TFIID 105 kD... 32 2.7
PFDB_PYRHO (O58268) Prefoldin beta subunit (GimC beta subunit) 32 3.6
VIT6_CAEEL (P18948) Vitellogenin 6 precursor 32 4.7
RX2_BRARE (O42357) Retinal homeobox protein Rx2 32 4.7
PGDS_RAT (P20786) Alpha platelet-derived growth factor receptor ... 32 4.7
HSF4_ARATH (Q96320) Heat shock factor protein 4 (HSF 4) (Heat sh... 32 4.7
GOA2_RAT (Q62839) Golgi autoantigen, golgin subfamily A member 2... 32 4.7
RADI_HUMAN (P35241) Radixin 31 6.1
PFDB_PYRAB (Q9UYJ4) Prefoldin beta subunit (GimC beta subunit) 31 6.1
PERI_XENLA (P48676) Peripherin (Neuronal intermediate filament IF3) 31 6.1
C5P2_HUMAN (Q96SN8) CDK5 regulatory subunit associated protein 2... 31 6.1
>MYSN_ACACA (P05659) Myosin II heavy chain, non muscle
Length = 1509
Score = 34.7 bits (78), Expect = 0.55
Identities = 20/79 (25%), Positives = 39/79 (49%), Gaps = 1/79 (1%)
Query: 392 REKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKDELLMRDSKRAR 451
++K+ +A+ + E+ + LDG+ + LK ++ + +R LEE + R K +
Sbjct: 1143 KQKKALEAKLTELEDQVTALDGQKNAAAAQAKTLKTQVDETKRRLEEAEASAARLEKERK 1202
Query: 452 GRRDFFARY-CDSDSESDN 469
D A+ D D+E D+
Sbjct: 1203 NALDEVAQLTADLDAERDS 1221
>RADI_CHICK (Q9PU45) Radixin
Length = 583
Score = 33.5 bits (75), Expect = 1.2
Identities = 27/95 (28%), Positives = 46/95 (48%), Gaps = 5/95 (5%)
Query: 364 VSSSTPSMPLPLLPATQDMYQEH---LAMESREKQVWKARYNQAENLIMTLDGRNEQKTH 420
+S+ P P P++P T++ + EH A S E +++E +T +NE+
Sbjct: 467 MSAPPPPPPPPVIPPTENEHDEHDENNAEASAELSSDGVMNHRSEEERVTETQKNERVKK 526
Query: 421 ENLMLKKELAKARRELE--EKDELLMRDSKRARGR 453
+ L ELA+AR E + + D L + K RG+
Sbjct: 527 QLQALSSELAQARDETKKTQNDVLHAENVKAGRGK 561
>PRX1_CHICK (Q91018) Homeobox prospero-like protein PROX1 (PROX 1)
Length = 736
Score = 33.5 bits (75), Expect = 1.2
Identities = 21/79 (26%), Positives = 38/79 (47%), Gaps = 15/79 (18%)
Query: 390 ESREKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKDELLMRDSKR 449
E++ KQ W + Q+ +++ R EQK E LK++L +++L + E
Sbjct: 206 ENKRKQNWPQQQQQSFQQLVS--ARKEQKREERRQLKQQLEDMQKQLRQLQE-------- 255
Query: 450 ARGRRDFFARYCDSDSESD 468
+F+ Y +DSE+D
Sbjct: 256 -----EFYQIYDSTDSEND 269
>LAP4_MOUSE (Q80U72) LAP4 protein (Scribble homolog protein)
Length = 1612
Score = 33.5 bits (75), Expect = 1.2
Identities = 28/132 (21%), Positives = 53/132 (39%), Gaps = 15/132 (11%)
Query: 349 DRAEEIGMPYPAMRYVSSSTPSMPLPL-------LPATQDMYQEHLAMESREKQVWKARY 401
D +E P+ + S+PS P PL + +QE L M+S E +
Sbjct: 1408 DDQQEAEQPWAVPSHAGGSSPSSPPPLGGNAPVRTAKAERRHQERLRMQSPELPAPERAL 1467
Query: 402 NQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKDELLMRDSKRARGRRDFFARYC 461
+ AE + + R + L+++ +A ++++ S+ RG+R R
Sbjct: 1468 SPAERRALEAEKRALWRAARMKSLEQDALRA--------QMVLSKSQEGRGKRGPLERLA 1519
Query: 462 DSDSESDNPPTT 473
++ S + P T
Sbjct: 1520 EAPSPAPTPSPT 1531
>TRHY_SHEEP (P22793) Trichohyalin
Length = 1549
Score = 32.7 bits (73), Expect = 2.1
Identities = 16/66 (24%), Positives = 33/66 (49%)
Query: 390 ESREKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKDELLMRDSKR 449
+ RE+Q + Q E + + ++ E L+KEL + L+E+++LL + ++
Sbjct: 614 QERERQYLEKVELQEEEQVQRQEREKRRQERERQYLEKELQRQEERLQEEEQLLREEREK 673
Query: 450 ARGRRD 455
R R+
Sbjct: 674 RRQERE 679
>IF2_AQUAE (O67825) Translation initiation factor IF-2
Length = 805
Score = 32.7 bits (73), Expect = 2.1
Identities = 16/73 (21%), Positives = 38/73 (51%)
Query: 378 ATQDMYQEHLAMESREKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELE 437
+ +++ +E L + +EK+ K + E + ++ + E++ E KKE K + ++
Sbjct: 127 SVEELIKEILEKKEKEKEKKKVEKERKEEKVRVVEVKKEERKEEKKEEKKEEEKPKIKMS 186
Query: 438 EKDELLMRDSKRA 450
+K+ +MR + A
Sbjct: 187 KKEREIMRKLEHA 199
>HOK1_MOUSE (Q8BIL5) Hook homolog 1
Length = 728
Score = 32.7 bits (73), Expect = 2.1
Identities = 20/60 (33%), Positives = 33/60 (54%), Gaps = 4/60 (6%)
Query: 378 ATQDMYQEHLAMESREKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELE 437
A Q ++ AME R +K +A N+I TLD + + E ++L+K+LA+ R +E
Sbjct: 591 ALQKKDEDMKAMEER----YKMYLEKARNVIKTLDPKLNPASAEIMLLRKQLAEKERRIE 646
>HOK1_HUMAN (Q9UJC3) Hook homolog 1 (h-hook1) (hHK1)
Length = 728
Score = 32.7 bits (73), Expect = 2.1
Identities = 20/60 (33%), Positives = 33/60 (54%), Gaps = 4/60 (6%)
Query: 378 ATQDMYQEHLAMESREKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELE 437
A Q ++ AME R +K +A N+I TLD + + E ++L+K+LA+ R +E
Sbjct: 591 ALQKKDEDMKAMEER----YKMYLEKARNVIKTLDPKLNPASAEIMLLRKQLAEKERRIE 646
>Y6G2_ENCCU (Q8SV63) Hypothetical protein ECU06_1620
Length = 579
Score = 32.3 bits (72), Expect = 2.7
Identities = 12/32 (37%), Positives = 24/32 (74%)
Query: 424 MLKKELAKARRELEEKDELLMRDSKRARGRRD 455
M+K+ + RE+E++ + L+RD ++A+GR+D
Sbjct: 302 MIKERQRRREREIEKRVKKLLRDEEKAKGRKD 333
>TA4B_HUMAN (Q92750) Transcription initiation factor TFIID 105 kDa
subunit (TAFII-105) (TAFII105) (Fragment)
Length = 801
Score = 32.3 bits (72), Expect = 2.7
Identities = 24/78 (30%), Positives = 40/78 (50%), Gaps = 4/78 (5%)
Query: 378 ATQDMYQEHLAMESREKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELE 437
ATQ+ + L + Q Y +EN I+ D R++ K E L +L K R++LE
Sbjct: 626 ATQERLRGLLEKLTAIAQHRMTTYKASENYILCSDTRSQLKFLEKL---DQLEKQRKDLE 682
Query: 438 EKDELLMRDSKRARGRRD 455
E+ E+L++ +K + D
Sbjct: 683 ER-EMLLKAAKSRSNKED 699
>PFDB_PYRHO (O58268) Prefoldin beta subunit (GimC beta subunit)
Length = 117
Score = 32.0 bits (71), Expect = 3.6
Identities = 22/67 (32%), Positives = 35/67 (51%), Gaps = 4/67 (5%)
Query: 381 DMYQEHLAMESREKQVWKARYNQAENLIMTL----DGRNEQKTHENLMLKKELAKARREL 436
D YQ+ L + ++KQ +A N+A+ + + D KT L++K KA +EL
Sbjct: 16 DTYQQQLQLVIQQKQKVQADLNEAKKALEEIETLPDDAQIYKTVGTLIVKTTKEKAVQEL 75
Query: 437 EEKDELL 443
+EK E L
Sbjct: 76 KEKIETL 82
>VIT6_CAEEL (P18948) Vitellogenin 6 precursor
Length = 1650
Score = 31.6 bits (70), Expect = 4.7
Identities = 24/95 (25%), Positives = 37/95 (38%), Gaps = 4/95 (4%)
Query: 386 HLAMESREKQVWKARYNQAENLIMTLDGRNEQKT----HENLMLKKELAKARRELEEKDE 441
HL+ R +Q + E D N++ T ++ + K RE+ EK+E
Sbjct: 1466 HLSALYRNQQCGLCGHYDNEKETEFYDAENQENTIPKFAKSYLYKDSKCNYEREMFEKEE 1525
Query: 442 LLMRDSKRARGRRDFFARYCDSDSESDNPPTTSYA 476
R K +D Y +S E D+ PT A
Sbjct: 1526 NFQRIEKNQEEEKDQEMNYEESRREQDDEPTEQVA 1560
>RX2_BRARE (O42357) Retinal homeobox protein Rx2
Length = 327
Score = 31.6 bits (70), Expect = 4.7
Identities = 34/118 (28%), Positives = 50/118 (41%), Gaps = 25/118 (21%)
Query: 13 DLVSLRELALKV-----KSQTGFRLRYGGLLTLLRTDVEEKLVHTLVQFYDPSFRCFTFP 67
D+ S ELA+KV + Q F+ R + +EK+ ++ +D R F P
Sbjct: 161 DVYSREELAMKVNLPEVRVQVWFQNRRA------KWRRQEKMDTGTMKLHDSPIRSFNRP 214
Query: 68 DFQLVPTLEAYSNLVGL------PIAEKTP------FTGPGTSLTPLVIAKDLYLKTS 113
+ P + SN + L P++ TP F GPG SL P A +L TS
Sbjct: 215 P--MAPNVGPMSNSLPLDPWLSSPLSSATPMHSIPGFMGPGQSLQPTYTAHPGFLNTS 270
>PGDS_RAT (P20786) Alpha platelet-derived growth factor receptor
precursor (EC 2.7.1.112) (PDGF-R-alpha)
Length = 1088
Score = 31.6 bits (70), Expect = 4.7
Identities = 30/107 (28%), Positives = 47/107 (43%), Gaps = 12/107 (11%)
Query: 33 RYGGLLTLLRTDVEEKLVHTLVQFYDPSFRCFTFPDFQLVPT--LEAYSNLVGLPIAEKT 90
RY L L+R E+ +T++ D + +TF LVP LE + G +
Sbjct: 373 RYQSKLKLIRAKEEDSGHYTIIVQNDDDMKSYTFELSTLVPASILELVDDHHGSGGGQTV 432
Query: 91 PFTGPGTSLTPL--VIAKDLYLKTSDVS--------NHLITKSHIRG 127
T GT L + +I KD+ +D S +++IT+ H RG
Sbjct: 433 RCTAEGTPLPNIEWMICKDIKKCNNDTSWTVLASNVSNIITEFHQRG 479
>HSF4_ARATH (Q96320) Heat shock factor protein 4 (HSF 4) (Heat shock
transcription factor 4) (HSTF 4)
Length = 284
Score = 31.6 bits (70), Expect = 4.7
Identities = 14/34 (41%), Positives = 21/34 (61%)
Query: 405 ENLIMTLDGRNEQKTHENLMLKKELAKARRELEE 438
EN++ L G NE+ EN L ELA A+++ +E
Sbjct: 150 ENMVADLSGENEKLKRENNNLSSELAAAKKQRDE 183
>GOA2_RAT (Q62839) Golgi autoantigen, golgin subfamily A member 2
(Cis-Golgi matrix protein GM130)
Length = 986
Score = 31.6 bits (70), Expect = 4.7
Identities = 20/66 (30%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Query: 373 LPLLPATQDMYQEHLAMESREKQVWKARYNQAENLIMTLDGRNEQKTHENLMLKKELAKA 432
L L +D Y E+L ES +W+ R Q + L EQ+ + L+ LA+
Sbjct: 374 LKQLQVERDQYAENLKGESA---MWQQRVQQMAEQVHALKEEKEQRESQVQELEASLAEL 430
Query: 433 RRELEE 438
R ++EE
Sbjct: 431 RSQMEE 436
>RADI_HUMAN (P35241) Radixin
Length = 583
Score = 31.2 bits (69), Expect = 6.1
Identities = 24/93 (25%), Positives = 43/93 (45%), Gaps = 3/93 (3%)
Query: 364 VSSSTPSMPLPLLPATQDMYQEH---LAMESREKQVWKARYNQAENLIMTLDGRNEQKTH 420
+S+ P P P++P T++ + EH A S E +++E +T +NE+
Sbjct: 467 MSAPPPPPPPPVIPPTENEHDEHDENNAEASAELSNEGVMNHRSEEERVTETQKNERVKK 526
Query: 421 ENLMLKKELAKARRELEEKDELLMRDSKRARGR 453
+ L ELA+AR E ++ ++ GR
Sbjct: 527 QLQALSSELAQARDETKKTQNDVLHAENVKAGR 559
>PFDB_PYRAB (Q9UYJ4) Prefoldin beta subunit (GimC beta subunit)
Length = 117
Score = 31.2 bits (69), Expect = 6.1
Identities = 21/67 (31%), Positives = 35/67 (51%), Gaps = 4/67 (5%)
Query: 381 DMYQEHLAMESREKQVWKARYNQAENLIMTL----DGRNEQKTHENLMLKKELAKARREL 436
+ YQ+ L + ++KQ +A N+A+ + + D KT L++K KA +EL
Sbjct: 16 ESYQQQLQLVIQQKQKVQADLNEAKKALEEIEALPDDAQVYKTVGTLIVKTTKEKALQEL 75
Query: 437 EEKDELL 443
+EK E L
Sbjct: 76 KEKVETL 82
>PERI_XENLA (P48676) Peripherin (Neuronal intermediate filament IF3)
Length = 456
Score = 31.2 bits (69), Expect = 6.1
Identities = 31/113 (27%), Positives = 51/113 (44%), Gaps = 22/113 (19%)
Query: 364 VSSSTP---SMPLPLLPATQDMYQEHLAMESREKQ------------VWKARYNQAENLI 408
V SSTP S+ + + QE L S EK + K RY + +N +
Sbjct: 56 VRSSTPVRVSLDRVDFSVAEAVNQEFLTTRSNEKAELQELNDRFASFIEKVRYLEQQNAV 115
Query: 409 MTLD---GRNEQKTHENLMLKKELAKARRELEEKDELLMRDSKRARGRRDFFA 458
+ + R+++ T + + ++EL REL ++ ELL +D + RD FA
Sbjct: 116 LVTEINQARSKEPTRASDLCQQEL----RELRKQLELLGKDRDHIQVERDNFA 164
>C5P2_HUMAN (Q96SN8) CDK5 regulatory subunit associated protein 2
(CDK5 activator-binding protein C48)
Length = 1893
Score = 31.2 bits (69), Expect = 6.1
Identities = 16/48 (33%), Positives = 26/48 (53%)
Query: 401 YNQAENLIMTLDGRNEQKTHENLMLKKELAKARRELEEKDELLMRDSK 448
Y E + G E N+ LK E+ +REL+E+++LL++ SK
Sbjct: 82 YFLEERMQQEFHGPTEHIYKTNIELKVEVESLKRELQEREQLLIKASK 129
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.136 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58,477,264
Number of Sequences: 164201
Number of extensions: 2596355
Number of successful extensions: 7071
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 7030
Number of HSP's gapped (non-prelim): 60
length of query: 476
length of database: 59,974,054
effective HSP length: 114
effective length of query: 362
effective length of database: 41,255,140
effective search space: 14934360680
effective search space used: 14934360680
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 68 (30.8 bits)
Medicago: description of AC149040.5