BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148996.15 + phase: 0
(153 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
H1E_STRPU (P19375) Histone H1, early embryonic 33 0.24
CNB3_HUMAN (Q9NQW8) Cyclic-nucleotide-gated cation channel beta ... 32 0.71
MDN1_HUMAN (Q9NU22) Midasin (MIDAS-containing protein) 31 0.92
DP3X_SALTY (P74876) DNA polymerase III subunit tau (EC 2.7.7.7) ... 31 0.92
H11_VOLCA (Q08864) Histone H1-I 31 1.2
NOT3_YEAST (P06102) General negative regulator of transcription ... 30 1.6
NFM_HUMAN (P07197) Neurofilament triplet M protein (160 kDa neur... 30 2.1
DCLY_SALTY (Q60002) Lysine decarboxylase, inducible (EC 4.1.1.18... 30 2.1
TOP1_YEAST (P04786) DNA topoisomerase I (EC 5.99.1.2) 30 2.7
VGP_MABVP (P35254) Structural glycoprotein precursor (Virion spi... 29 3.5
VGP_MABVM (P35253) Structural glycoprotein precursor (Virion spi... 29 3.5
PNUT_DROME (P40797) Peanut protein 29 3.5
IF5_SCHPO (Q09689) Probable eukaryotic translation initiation fa... 29 3.5
CIN7_HUMAN (Q01118) Sodium channel protein type VII alpha subuni... 29 3.5
HEM6_PROMP (Q7UZS3) Coproporphyrinogen III oxidase, aerobic (EC ... 29 4.6
ATRX_CAEEL (Q9U7E0) Transcriptional regulator ATRX homolog (X-li... 29 4.6
TRDN_CANFA (P82179) Triadin 28 6.0
IF2_STRA5 (Q8E1H3) Translation initiation factor IF-2 28 6.0
CCAS_RABIT (P07293) Voltage-dependent L-type calcium channel alp... 28 6.0
CCAS_HUMAN (Q13698) Voltage-dependent L-type calcium channel alp... 28 6.0
>H1E_STRPU (P19375) Histone H1, early embryonic
Length = 205
Score = 33.1 bits (74), Expect = 0.24
Identities = 25/87 (28%), Positives = 43/87 (48%), Gaps = 9/87 (10%)
Query: 15 SNGGFFVTISAILALLSRKANRLKEKAKSSSTTKPIRD----EEWRFDLKTPPKSPMAKP 70
++G F V + A A S KA + KEKAK + + ++ EE + PK A P
Sbjct: 83 ASGSFKVNVQAAKAQASEKAKKEKEKAKLLAQREKAKEKGCSEEGETAEGSRPKKVKAAP 142
Query: 71 KKLLSNISNKALSQFGKKKQREEREKE 97
KK + K + + +KK++++ K+
Sbjct: 143 KK-----AKKPVKKTTEKKEKKKTPKK 164
>CNB3_HUMAN (Q9NQW8) Cyclic-nucleotide-gated cation channel beta 3
(CNG channel beta 3) (Cyclic nucleotide gated channel
beta 3) (Cone photoreceptor cGMP-gated channel beta
subunit) (Cyclic nucleotide-gated cation channel
modulatory subunit)
Length = 809
Score = 31.6 bits (70), Expect = 0.71
Identities = 25/77 (32%), Positives = 38/77 (48%), Gaps = 6/77 (7%)
Query: 29 LLSRKAN-RLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKP-KKLLSNISNKALSQFG 86
+L +KA LK+KAK++ T P +D L PPK K K LL +L++
Sbjct: 637 ILMKKARVLLKQKAKTAEATPPRKD----LALLFPPKEETPKLFKTLLGGTGKASLARLL 692
Query: 87 KKKQREEREKEGWGNGG 103
K K+ + +K+ GG
Sbjct: 693 KLKREQAAQKKENSEGG 709
>MDN1_HUMAN (Q9NU22) Midasin (MIDAS-containing protein)
Length = 5596
Score = 31.2 bits (69), Expect = 0.92
Identities = 26/83 (31%), Positives = 37/83 (44%), Gaps = 12/83 (14%)
Query: 29 LLSRKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSP---------MAKPKKLLSNISN 79
L +A++L E+ + +EE D KT P +AK L S SN
Sbjct: 4766 LNGEEADKLDERLWGDDDEEEDEEEE---DNKTEETGPGMDEEDSELVAKDDNLDSGNSN 4822
Query: 80 KALSQFGKKKQREEREKEGWGNG 102
K SQ KK+++EE E + G G
Sbjct: 4823 KDKSQQDKKEEKEEAEADDGGQG 4845
>DP3X_SALTY (P74876) DNA polymerase III subunit tau (EC 2.7.7.7)
[Contains: DNA polymerase III subunit gamma]
Length = 642
Score = 31.2 bits (69), Expect = 0.92
Identities = 22/82 (26%), Positives = 35/82 (41%), Gaps = 3/82 (3%)
Query: 21 VTISAILALLSRKANRLKEKAKSSSTTKPIRDEEWRFDLKTP---PKSPMAKPKKLLSNI 77
V SA+ L S A S+ T P++ E +R+ TP K +A PK L +
Sbjct: 441 VNNSALERLASVSERVQARPAPSALETAPVKKEAYRWKATTPVVQTKEVVATPKALKKAL 500
Query: 78 SNKALSQFGKKKQREEREKEGW 99
++ + K E E++ W
Sbjct: 501 EHEKTPELAAKLAAEAIERDPW 522
>H11_VOLCA (Q08864) Histone H1-I
Length = 260
Score = 30.8 bits (68), Expect = 1.2
Identities = 25/80 (31%), Positives = 37/80 (46%), Gaps = 10/80 (12%)
Query: 17 GGFFVTISAILALLSRKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSN 76
GG V + L + LK KAK S+ K D E + PK AKPKK +
Sbjct: 114 GGKLVKVKGSFKL----SEALKAKAKKSTPKKAKADGEAK------PKKSEAKPKKAEAV 163
Query: 77 ISNKALSQFGKKKQREEREK 96
KA + ++ ++E++EK
Sbjct: 164 KKTKAPKEKVERPKKEKKEK 183
Score = 28.9 bits (63), Expect = 4.6
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 5/74 (6%)
Query: 32 RKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPM---AKPKKLLSNISNKALSQFGKK 88
+KA EK K ++T K ++ K PKSP AKPKK + S KA +
Sbjct: 187 KKATPKAEKPKKAATPKSAGKKKATPKPKAAPKSPAKKDAKPKK--ATPSKKAAPKKAPA 244
Query: 89 KQREEREKEGWGNG 102
K+ + KE G
Sbjct: 245 KKSTPKAKEAKSKG 258
>NOT3_YEAST (P06102) General negative regulator of transcription
subunit 3
Length = 836
Score = 30.4 bits (67), Expect = 1.6
Identities = 20/69 (28%), Positives = 33/69 (46%), Gaps = 4/69 (5%)
Query: 32 RKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSNISNKALSQFGKKKQR 91
+K RL+E+ KS ++ I+D++ D + + M K K + KA S KK
Sbjct: 53 KKLQRLREQIKSWQSSPDIKDKDSLLDYRRSVEIAMEKYKAVEKASKEKAYSNISLKKSE 112
Query: 92 ----EEREK 96
+ERE+
Sbjct: 113 TLDPQERER 121
>NFM_HUMAN (P07197) Neurofilament triplet M protein (160 kDa
neurofilament protein) (Neurofilament medium
polypeptide) (NF-M) (Neurofilament 3)
Length = 915
Score = 30.0 bits (66), Expect = 2.1
Identities = 20/62 (32%), Positives = 34/62 (54%), Gaps = 15/62 (24%)
Query: 37 LKEKAKSSSTTKPIRDEEWRFDLKTP-PKSPMAKPKKLLSNISNKALSQFGKKKQREERE 95
++EK KS + P+ ++ K+P PKSP+ + K + ++ GK +Q+EE E
Sbjct: 660 VEEKGKSPVSKSPVEEKA-----KSPVPKSPVEEAK---------SKAEVGKGEQKEEEE 705
Query: 96 KE 97
KE
Sbjct: 706 KE 707
>DCLY_SALTY (Q60002) Lysine decarboxylase, inducible (EC 4.1.1.18)
(LDC)
Length = 714
Score = 30.0 bits (66), Expect = 2.1
Identities = 17/48 (35%), Positives = 29/48 (60%), Gaps = 2/48 (4%)
Query: 71 KKLLSNISNKALSQFGKKKQREEREKEGWGNGGVWQKEILMGGKCEPL 118
K+L++ +A+ +F K+ +R + E +GW VWQ E + G +C PL
Sbjct: 422 KRLINGSIERAI-KFRKEIKRLKSESDGWFFD-VWQPEHIDGAECWPL 467
>TOP1_YEAST (P04786) DNA topoisomerase I (EC 5.99.1.2)
Length = 769
Score = 29.6 bits (65), Expect = 2.7
Identities = 21/71 (29%), Positives = 36/71 (50%), Gaps = 12/71 (16%)
Query: 35 NRLKEKAKSSSTTKPIRDEEWRFDLKTPP--KSPMAKPKKL------LSNISNKALSQFG 86
+++ +K T+P++ L +PP KS +KPKK+ + + K Q
Sbjct: 54 SKISKKKTKKIKTEPVQSSS----LPSPPAKKSATSKPKKIKKEDGDVKVKTTKKEEQEN 109
Query: 87 KKKQREEREKE 97
+KK+REE E+E
Sbjct: 110 EKKKREEEEEE 120
>VGP_MABVP (P35254) Structural glycoprotein precursor (Virion spike
glycoprotein)
Length = 681
Score = 29.3 bits (64), Expect = 3.5
Identities = 11/32 (34%), Positives = 18/32 (55%)
Query: 74 LSNISNKALSQFGKKKQREEREKEGWGNGGVW 105
+ ++S Q + K+ E++E GWG GG W
Sbjct: 613 IEDLSRNISEQIDQIKKDEQKEGTGWGLGGKW 644
>VGP_MABVM (P35253) Structural glycoprotein precursor (Virion spike
glycoprotein)
Length = 681
Score = 29.3 bits (64), Expect = 3.5
Identities = 11/32 (34%), Positives = 18/32 (55%)
Query: 74 LSNISNKALSQFGKKKQREEREKEGWGNGGVW 105
+ ++S Q + K+ E++E GWG GG W
Sbjct: 613 IEDLSKNISEQIDQIKKDEQKEGTGWGLGGKW 644
>PNUT_DROME (P40797) Peanut protein
Length = 539
Score = 29.3 bits (64), Expect = 3.5
Identities = 27/102 (26%), Positives = 45/102 (43%), Gaps = 14/102 (13%)
Query: 9 SPNRHHSNGGFFVTISAILALLSRKANRLKEKAKSSSTTKPIRDEEWRFDL--------K 60
SP + NGG ISA+ + L++ A R K++A S+S + L
Sbjct: 3 SPRSNAVNGGSGGAISALPSTLAQLALRDKQQAASASASSATNGSSGSESLVGVGGRPPN 62
Query: 61 TPPKSPMAKPKKL------LSNISNKALSQFGKKKQREEREK 96
PP P+A KL SN + L+ ++K+ ++ +K
Sbjct: 63 QPPSVPVAASGKLDTSSGGASNGDSNKLTHDLQEKEHQQAQK 104
>IF5_SCHPO (Q09689) Probable eukaryotic translation initiation
factor 5 (eIF-5)
Length = 395
Score = 29.3 bits (64), Expect = 3.5
Identities = 17/73 (23%), Positives = 37/73 (50%), Gaps = 3/73 (4%)
Query: 36 RLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSNISNKALSQFGKKKQREERE 95
R++++A T + + DE+W D T ++ A+ ++L N+ + +L+ + EE E
Sbjct: 175 RIRQEAAELPTAEVVNDEDWAVD--TSEEAVRARVQELEGNMKD-SLTLSDLRGDEEEAE 231
Query: 96 KEGWGNGGVWQKE 108
+ G W ++
Sbjct: 232 SSRYDQFGEWLED 244
>CIN7_HUMAN (Q01118) Sodium channel protein type VII alpha subunit
(Putative voltage-gated sodium channel alpha subunit
Nax) (Sodium channel protein, cardiac and skeletal
muscle alpha-subunit)
Length = 1682
Score = 29.3 bits (64), Expect = 3.5
Identities = 21/74 (28%), Positives = 36/74 (48%), Gaps = 3/74 (4%)
Query: 27 LALLSRKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKS---PMAKPKKLLSNISNKALS 83
L+ LS + LK+K KSS T K + E + + +P S P+A + + N+ NK +
Sbjct: 795 LSELSNTQDFLKDKEKSSGTEKNATENESQSLIPSPSVSETVPIASGESDIENLDNKEIQ 854
Query: 84 QFGKKKQREEREKE 97
+E+ K+
Sbjct: 855 SKSGDGGSKEKIKQ 868
>HEM6_PROMP (Q7UZS3) Coproporphyrinogen III oxidase, aerobic (EC
1.3.3.3) (Coproporphyrinogenase) (Coprogen oxidase)
Length = 342
Score = 28.9 bits (63), Expect = 4.6
Identities = 24/77 (31%), Positives = 36/77 (46%), Gaps = 8/77 (10%)
Query: 60 KTPPKSPMAKPKKLLSNISN---KALSQF-GKKKQREEREKEGWGNGGVWQKEILMGGKC 115
K PPK+ K K LL + + K L GK K EE G GG + +L G
Sbjct: 3 KEPPKNSREKTKNLLLKLQDNICKGLENIDGKAKFTEESWLREEGGGG--KSRVLKNGSI 60
Query: 116 EPLDFSGVIYYDINGKQ 132
+ +GV + +++GK+
Sbjct: 61 --FEQAGVNFSEVHGKE 75
>ATRX_CAEEL (Q9U7E0) Transcriptional regulator ATRX homolog
(X-linked nuclear protein-1)
Length = 1359
Score = 28.9 bits (63), Expect = 4.6
Identities = 19/60 (31%), Positives = 30/60 (49%), Gaps = 1/60 (1%)
Query: 38 KEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSNISNKALSQFGK-KKQREEREK 96
KEK+K TT DE+ + + K K KK S+ S++ + K KK ++ +EK
Sbjct: 115 KEKSKKKRTTSSSEDEDSDEEREQKSKKKSKKTKKQTSSESSEESEEERKVKKSKKNKEK 174
>TRDN_CANFA (P82179) Triadin
Length = 700
Score = 28.5 bits (62), Expect = 6.0
Identities = 16/58 (27%), Positives = 27/58 (45%)
Query: 38 KEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSNISNKALSQFGKKKQREERE 95
KEK+ +ST ++ E + D K P KPK S + + S+ KK+ + +
Sbjct: 452 KEKSGKTSTASKDKEPEIKKDEKMPKADKEVKPKPPQSQVKKEEKSESQVKKEAKPEQ 509
>IF2_STRA5 (Q8E1H3) Translation initiation factor IF-2
Length = 927
Score = 28.5 bits (62), Expect = 6.0
Identities = 17/71 (23%), Positives = 33/71 (45%), Gaps = 2/71 (2%)
Query: 33 KANRLKEKAKSSSTTKPIRDEEWRFD--LKTPPKSPMAKPKKLLSNISNKALSQFGKKKQ 90
KA +KAK +KP+ + + TP ++ ++ KK + ++ S + Q
Sbjct: 233 KAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQTRSDKSRQFSNENEDGQ 292
Query: 91 REEREKEGWGN 101
++ R K+ W N
Sbjct: 293 KQTRNKKNWNN 303
>CCAS_RABIT (P07293) Voltage-dependent L-type calcium channel
alpha-1S subunit (Voltage-gated calcium channel alpha
subunit Cav1.1) (Calcium channel, L type, alpha-1
polypeptide, isoform 3, skeletal muscle)
Length = 1873
Score = 28.5 bits (62), Expect = 6.0
Identities = 13/38 (34%), Positives = 26/38 (68%), Gaps = 1/38 (2%)
Query: 17 GGFFVTISAILALLSRKANRLKEKAKSSSTTKPIRDEE 54
G FF+ ++ +L +LS + + +EKAKS T + +R+++
Sbjct: 321 GSFFI-LNLVLGVLSGEFTKEREKAKSRGTFQKLREKQ 357
>CCAS_HUMAN (Q13698) Voltage-dependent L-type calcium channel
alpha-1S subunit (Voltage-gated calcium channel alpha
subunit Cav1.1) (Calcium channel, L type, alpha-1
polypeptide, isoform 3, skeletal muscle)
Length = 1873
Score = 28.5 bits (62), Expect = 6.0
Identities = 13/38 (34%), Positives = 26/38 (68%), Gaps = 1/38 (2%)
Query: 17 GGFFVTISAILALLSRKANRLKEKAKSSSTTKPIRDEE 54
G FF+ ++ +L +LS + + +EKAKS T + +R+++
Sbjct: 321 GSFFI-LNLVLGVLSGEFTKEREKAKSRGTFQKLREKQ 357
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.313 0.133 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,467,150
Number of Sequences: 164201
Number of extensions: 791247
Number of successful extensions: 1924
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 1892
Number of HSP's gapped (non-prelim): 44
length of query: 153
length of database: 59,974,054
effective HSP length: 101
effective length of query: 52
effective length of database: 43,389,753
effective search space: 2256267156
effective search space used: 2256267156
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC148996.15