BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148995.1 + phase: 0
(220 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YDC3_PSEAE (P48636) Hypothetical protein PA4923 173 2e-43
YJF5_YEAST (P47044) Hypothetical 26.9 kDa protein in BTN1-PEP8 i... 158 8e-39
FAS6_RHOFA (P46378) Hypothetical 21.1 kDa protein in fasciation ... 150 2e-36
YGDH_ECOLI (P37350) Hypothetical protein ygdH 38 0.021
G3PG_LEIME (Q27890) Glyceraldehyde-3-phosphate dehydrogenase, gl... 36 0.081
G3PG_CRIFA (O96423) Glyceraldehyde-3-phosphate dehydrogenase, gl... 36 0.081
G3PG_TRYCR (P22513) Glyceraldehyde-3-phosphate dehydrogenase, gl... 34 0.23
TIG_SYNP7 (Q935Z3) Trigger factor (TF) 33 0.68
SYM_ORYSA (Q9ZTS1) Probable methionyl-tRNA synthetase (EC 6.1.1.... 32 1.2
FTSZ_SODGL (Q9ALA4) Cell division protein ftsZ 31 2.0
SYHH_HUMAN (P49590) Histidyl-tRNA synthetase homolog (EC 6.1.1.2... 30 3.4
Y169_HELPY (P56113) Putative protease HP0169 precursor (EC 3.4.-.-) 30 5.8
DIM_PEA (P93472) Cell elongation protein diminuto 30 5.8
CHMO_AMATR (Q93XE1) Choline monooxygenase, chloroplast precursor... 30 5.8
HIS4_CORDI (P60580) 1-(5-phosphoribosyl)-5-[(5-phosphoribosylami... 29 7.5
G3PG_TRYBB (P22512) Glyceraldehyde-3-phosphate dehydrogenase, gl... 29 7.5
ABC6_HUMAN (Q9NP58) ATP-binding cassette, sub-family B, member 6... 29 7.5
SYGB_HAEIN (P43822) Glycyl-tRNA synthetase beta chain (EC 6.1.1.... 29 9.9
PPNK_CHLTE (Q8KG83) Probable inorganic polyphosphate/ATP-NAD kin... 29 9.9
ILVD_BACHD (Q9K8E4) Dihydroxy-acid dehydratase (EC 4.2.1.9) (DAD) 29 9.9
>YDC3_PSEAE (P48636) Hypothetical protein PA4923
Length = 195
Score = 173 bits (439), Expect = 2e-43
Identities = 84/180 (46%), Positives = 119/180 (65%)
Query: 14 KRICVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGRHVI 73
+ +CVFCG+SPG YQ+AA+ LG L R + LVYGGG++GLMG V+ A G VI
Sbjct: 4 RSVCVFCGASPGASPVYQEAAVALGRHLAERGLTLVYGGGAVGLMGTVADAALAAGGEVI 63
Query: 74 GVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVITWA 133
G+IP++L E+ + + ++ V MH RKA MA+ +DAFIALPGG GTLEEL EV TW
Sbjct: 64 GIIPQSLQEAEIGHKGLTRLEVVDGMHARKARMAELADAFIALPGGLGTLEELFEVWTWG 123
Query: 134 QLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTAKELVKKLEEYVP 193
QLG H KP+GL+ V+G+++ LL+F+D V+E F+ R ++ + + L+ L + P
Sbjct: 124 QLGYHAKPLGLLEVNGFYDPLLTFLDHLVDERFVRAEHRGMLQRGASPEALLDALAAWTP 183
>YJF5_YEAST (P47044) Hypothetical 26.9 kDa protein in BTN1-PEP8
intergenic region
Length = 245
Score = 158 bits (400), Expect = 8e-39
Identities = 90/216 (41%), Positives = 123/216 (56%), Gaps = 29/216 (13%)
Query: 3 SRNGEIKLSKFKRICVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVS 62
SR G++ K +CV+CGSS G K Y ++A LG LVYGGG+ GLMG ++
Sbjct: 11 SRGGQVG---GKSVCVYCGSSFGAKALYSESAEELGALFHKLGWKLVYGGGTTGLMGKIA 67
Query: 63 QAVH--DGGRHVIGVIPKTLMPRELT------------------------GETVGEVKAV 96
++ D V G+IP L+ +E T E GE V
Sbjct: 68 RSTMGPDLSGQVHGIIPNALVSKERTDEDKEDVNKALLESVENHKGATPISEEYGETTIV 127
Query: 97 ADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVITWAQLGIHDKPVGLVNVDGYFNSLLS 156
DMH RK MA SDAF+A+PGGYGT EE++E ITW+QLGIH+KP+ L N+DG+++ LL
Sbjct: 128 PDMHTRKRMMANLSDAFVAMPGGYGTFEEIMECITWSQLGIHNKPIILFNIDGFYDKLLE 187
Query: 157 FIDKAVEEGFISPNARHIIVSAPTAKELVKKLEEYV 192
F+ +++E FIS II A T +E+V K+E+YV
Sbjct: 188 FLKHSIQERFISVKNGEIIQVASTPQEVVDKIEKYV 223
>FAS6_RHOFA (P46378) Hypothetical 21.1 kDa protein in fasciation
locus (ORF6)
Length = 198
Score = 150 bits (380), Expect = 2e-36
Identities = 78/165 (47%), Positives = 105/165 (63%), Gaps = 3/165 (1%)
Query: 14 KRICVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGRHVI 73
K + VFCG+ PG+ T Y A +G + + LVYGG +GLMG ++ A D G V+
Sbjct: 20 KSVTVFCGAMPGRGTKYGQLAEGMGRAIARSKLRLVYGGARVGLMGTLANAALDSGGTVV 79
Query: 74 GVIPK--TLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVIT 131
GVIP+ T +P E + E+ V DMHQRKA MA+ DAFIALPGG GT EE EV+T
Sbjct: 80 GVIPESFTAIP-EAAHHGLTELHVVHDMHQRKALMAELGDAFIALPGGVGTAEEFFEVLT 138
Query: 132 WAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIV 176
W+ LG+H+KP L+N + Y+ LLS+I+ A EGFI+P R ++
Sbjct: 139 WSHLGLHNKPCVLLNDNEYYRPLLSYIEHAAVEGFITPATRSRVI 183
>YGDH_ECOLI (P37350) Hypothetical protein ygdH
Length = 454
Score = 37.7 bits (86), Expect = 0.021
Identities = 46/198 (23%), Positives = 75/198 (37%), Gaps = 36/198 (18%)
Query: 34 AMNLGNELVSRNIDLVYGGGSIGL--------MGLVSQAVHDGGRHVIGVIPKTLMPREL 85
A +GN+L R +++ G G + +G Q D IG+ +++ E
Sbjct: 167 ARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDS--RFIGMTEPSIIAAEP 224
Query: 86 TGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVITWAQLGIHDKP---- 141
V E+ + D+ +R + + I PGG GT EELL + LGI P
Sbjct: 225 PNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYL-----LGILMNPANKD 279
Query: 142 -------VGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTAKELVKKLEEYVPC 194
G YF L F+ + E RH + A E+ +++++ +P
Sbjct: 280 QVLPLILTGPKESADYFRVLDEFVVHTLGEN----ARRHYRIIIDDAAEVARQMKKSMPL 335
Query: 195 ------HEGVASKLSWQM 206
G A +W M
Sbjct: 336 VKENRRDTGDAYSFNWSM 353
>G3PG_LEIME (Q27890) Glyceraldehyde-3-phosphate dehydrogenase,
glycosomal (EC 1.2.1.12) (GAPDH)
Length = 360
Score = 35.8 bits (81), Expect = 0.081
Identities = 27/76 (35%), Positives = 34/76 (44%), Gaps = 5/76 (6%)
Query: 133 AQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPT---AKELVKKL- 188
AQ D P G + VD S F DK EG I A+ +++SAP AK +V +
Sbjct: 90 AQRNPADLPWGKLGVDYVIESTGLFTDKLKAEGHIKGGAKKVVISAPASGGAKTIVMGVN 149
Query: 189 -EEYVPCHEGVASKLS 203
EY P V S S
Sbjct: 150 QHEYSPASHHVVSNAS 165
>G3PG_CRIFA (O96423) Glyceraldehyde-3-phosphate dehydrogenase,
glycosomal (EC 1.2.1.12) (GAPDH)
Length = 360
Score = 35.8 bits (81), Expect = 0.081
Identities = 25/70 (35%), Positives = 33/70 (46%), Gaps = 5/70 (7%)
Query: 139 DKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPT---AKELVKKL--EEYVP 193
D P G + VD S F DKA EG + A+ +++SAP AK +V + EY P
Sbjct: 96 DLPWGKLGVDYVIESTGLFTDKAKAEGHVKGGAKKVVISAPASGGAKTIVMGVNQHEYNP 155
Query: 194 CHEGVASKLS 203
V S S
Sbjct: 156 ATHHVVSNAS 165
>G3PG_TRYCR (P22513) Glyceraldehyde-3-phosphate dehydrogenase,
glycosomal (EC 1.2.1.12) (GAPDH)
Length = 359
Score = 34.3 bits (77), Expect = 0.23
Identities = 27/76 (35%), Positives = 34/76 (44%), Gaps = 5/76 (6%)
Query: 133 AQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPT---AKELVKKL- 188
AQ D P G + V+ S F KA EG + AR +++SAP AK LV +
Sbjct: 90 AQRNPADLPWGKLGVEYVIESTGLFTAKAAAEGHLRGGARKVVISAPASGGAKTLVMGVN 149
Query: 189 -EEYVPCHEGVASKLS 203
EY P V S S
Sbjct: 150 HHEYNPSEHHVVSNAS 165
>TIG_SYNP7 (Q935Z3) Trigger factor (TF)
Length = 474
Score = 32.7 bits (73), Expect = 0.68
Identities = 25/111 (22%), Positives = 45/111 (40%), Gaps = 3/111 (2%)
Query: 57 LMGLVSQAVHDGGRHVIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIAL 116
+ G+V A+ + R V P+T E+ G+ + ++ R ++ + DAF
Sbjct: 211 ISGIVGMAIEET-RTVDATFPETYAQEEVAGKAAQFTITLKELKTR--DLPELDDAFAQE 267
Query: 117 PGGYGTLEELLEVITWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFI 167
Y T+EEL +T H+ V D +L +D + E +
Sbjct: 268 ASQYETIEELKTALTERFQAEHESEVKASKRDAILTALADQLDVEIPESLL 318
>SYM_ORYSA (Q9ZTS1) Probable methionyl-tRNA synthetase (EC 6.1.1.10)
(Methionine--tRNA ligase) (MetRS)
Length = 804
Score = 32.0 bits (71), Expect = 1.2
Identities = 23/78 (29%), Positives = 36/78 (45%), Gaps = 5/78 (6%)
Query: 129 VITWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFIS-----PNARHIIVSAPTAKE 183
+ TWA L L N+ + N +LSF+ K G+ S PNA ++ A++
Sbjct: 389 LFTWADLQAKLNSELLNNLGNFINRVLSFVAKPAGAGYDSIVPDAPNAESHPLTKALAEK 448
Query: 184 LVKKLEEYVPCHEGVASK 201
K +E+Y+ E V K
Sbjct: 449 TNKWVEQYLEAMEKVKLK 466
>FTSZ_SODGL (Q9ALA4) Cell division protein ftsZ
Length = 386
Score = 31.2 bits (69), Expect = 2.0
Identities = 29/124 (23%), Positives = 53/124 (42%), Gaps = 21/124 (16%)
Query: 51 GGGSIGLMGLVSQAVHDGGRHVIGVIPKTLMPRELTGETVGEVKAVADMHQRKAEMAKHS 110
GG G +V++ D G + V+ K P G+ K +A Q AE++KH
Sbjct: 106 GGTGTGAAPVVAEVAKDQGILTVAVVAK---PFNFEGK-----KRMAFAEQGIAELSKHV 157
Query: 111 DAFIALPGGYGTLEELLEVITWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPN 170
D+ I +P ++LL+V+ + + L++ G N +L + + E P
Sbjct: 158 DSLITIPN-----DKLLKVL--------GRGISLLDAFGAANDVLKGAVQGIAELITRPG 204
Query: 171 ARHI 174
++
Sbjct: 205 LMNV 208
>SYHH_HUMAN (P49590) Histidyl-tRNA synthetase homolog (EC 6.1.1.21)
(Histidine--tRNA ligase homolog) (HisRS)
Length = 506
Score = 30.4 bits (67), Expect = 3.4
Identities = 25/89 (28%), Positives = 47/89 (52%), Gaps = 14/89 (15%)
Query: 123 LEELLEVITWAQLG-----IHDKPV--GLVNVDGY----FNSLLSFIDKAVEEGFISPNA 171
L+ + E+++ QLG ++D+ + G+ V G F ++ S IDK + + +
Sbjct: 193 LKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAW--KDV 250
Query: 172 RH-IIVSAPTAKELVKKLEEYVPCHEGVA 199
RH ++V A E+ ++ +YV CH GV+
Sbjct: 251 RHEMVVKKGLAPEVADRIGDYVQCHGGVS 279
>Y169_HELPY (P56113) Putative protease HP0169 precursor (EC 3.4.-.-)
Length = 422
Score = 29.6 bits (65), Expect = 5.8
Identities = 24/93 (25%), Positives = 48/93 (50%), Gaps = 7/93 (7%)
Query: 122 TLEELLEVITWAQLGIHDKPVGLVNVDGY-FNSLLSFIDKAVEE-GFISPNARHIIVSAP 179
TLE E I +A ++ K +N G+ FNS L +++ +++ + P+A I++AP
Sbjct: 45 TLETFKEGIDYAH-ALNKKVYATIN--GFPFNSQLKLLEEHIDKMAELEPDA--FIIAAP 99
Query: 180 TAKELVKKLEEYVPCHEGVASKLSWQMEQQLAY 212
+L K+ ++P H + + ++ Q+ Y
Sbjct: 100 GVVKLALKIAPHIPIHLSTQANVLNLLDAQVFY 132
>DIM_PEA (P93472) Cell elongation protein diminuto
Length = 567
Score = 29.6 bits (65), Expect = 5.8
Identities = 26/89 (29%), Positives = 38/89 (42%), Gaps = 15/89 (16%)
Query: 35 MNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGRHVIGVIPKTLMPRELTGETVGEVK 94
MNL +V+ DL GG L++ +G H G+ T++ E+ VK
Sbjct: 145 MNLALAVVAELDDLTVGG-------LINGYGIEGSSHKYGLFSDTVVAFEIILADGSLVK 197
Query: 95 AVADMHQRKAEMAKHSDAFIALPGGYGTL 123
A D ++SD F A+P GTL
Sbjct: 198 ATKDN--------EYSDLFYAIPWSQGTL 218
>CHMO_AMATR (Q93XE1) Choline monooxygenase, chloroplast precursor
(EC 1.14.15.7)
Length = 442
Score = 29.6 bits (65), Expect = 5.8
Identities = 18/75 (24%), Positives = 32/75 (42%), Gaps = 3/75 (4%)
Query: 145 VNVDGYFNSLLSFIDKAVEEGFI--SPNARHIIVSAPTAKELVKKLEEYVPCHEGVASKL 202
+N+ + I KAV I S N+ +I + P K ++ + + VP +G
Sbjct: 43 INISKITSKTNKIIPKAVASPVIPSSINSNNITTTTPNIKRIIHEFDPKVPAEDGFTPPS 102
Query: 203 SWQMEQQLAYPQDYD 217
+W + L Y + D
Sbjct: 103 TWYTDPSL-YSHELD 116
>HIS4_CORDI (P60580)
1-(5-phosphoribosyl)-5-[(5-
phosphoribosylamino)methylideneamino]
imidazole-4-carboxamide isomerase (EC 5.3.1.16)
(Phosphoribosylformimino-5-aminoimidazole carboxamide
ribotide isomerase)
Length = 242
Score = 29.3 bits (64), Expect = 7.5
Identities = 14/47 (29%), Positives = 25/47 (52%)
Query: 105 EMAKHSDAFIALPGGYGTLEELLEVITWAQLGIHDKPVGLVNVDGYF 151
+++ +DA + GG TLE++LE+ + GI +G +G F
Sbjct: 187 DVSAATDAKVVASGGISTLEDVLELARYEDEGIDSAIIGKALYEGRF 233
>G3PG_TRYBB (P22512) Glyceraldehyde-3-phosphate dehydrogenase,
glycosomal (EC 1.2.1.12) (GAPDH)
Length = 358
Score = 29.3 bits (64), Expect = 7.5
Identities = 24/76 (31%), Positives = 33/76 (42%), Gaps = 5/76 (6%)
Query: 133 AQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPT---AKELVKKL- 188
AQ D P G + V+ S F K+ EG + AR +++SAP AK V +
Sbjct: 89 AQRNPADLPWGKLGVEYVIESTGLFTVKSAAEGHLRGGARKVVISAPASGGAKTFVMGVN 148
Query: 189 -EEYVPCHEGVASKLS 203
Y P + V S S
Sbjct: 149 HNNYNPREQHVVSNAS 164
>ABC6_HUMAN (Q9NP58) ATP-binding cassette, sub-family B, member 6,
mitochondrial precursor (Mitochondrial ABC transporter
3) (Mt-ABC transporter 3) (ABC transporter umat)
Length = 842
Score = 29.3 bits (64), Expect = 7.5
Identities = 21/65 (32%), Positives = 32/65 (48%), Gaps = 3/65 (4%)
Query: 61 VSQAVHDGGRHVIGVIPK-TLMPRELTGETV--GEVKAVADMHQRKAEMAKHSDAFIALP 117
+SQ R IGV+P+ T++ + + + G V A D + A+ A DA +A P
Sbjct: 654 ISQVTQASLRSHIGVVPQDTVLFNDTIADNIRYGRVTAGNDEVEAAAQAAGIHDAIMAFP 713
Query: 118 GGYGT 122
GY T
Sbjct: 714 EGYRT 718
>SYGB_HAEIN (P43822) Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)
(Glycine--tRNA ligase beta chain) (GlyRS)
Length = 688
Score = 28.9 bits (63), Expect = 9.9
Identities = 17/37 (45%), Positives = 22/37 (58%), Gaps = 1/37 (2%)
Query: 96 VADMHQRKAEMAKHSDAFIALPGGYGTLEE-LLEVIT 131
VAD ++RKAE+ S A GG +EE LLE +T
Sbjct: 216 VADFNERKAEILAKSQAKATALGGVADIEESLLEEVT 252
>PPNK_CHLTE (Q8KG83) Probable inorganic polyphosphate/ATP-NAD kinase
(EC 2.7.1.23) (Poly(P)/ATP NAD kinase)
Length = 283
Score = 28.9 bits (63), Expect = 9.9
Identities = 13/23 (56%), Positives = 19/23 (82%), Gaps = 1/23 (4%)
Query: 101 QRKAEMAKHSDAFIALPGGYGTL 123
+ KA++++H DAF+AL GG GTL
Sbjct: 48 EEKADLSQHCDAFVAL-GGDGTL 69
>ILVD_BACHD (Q9K8E4) Dihydroxy-acid dehydratase (EC 4.2.1.9) (DAD)
Length = 555
Score = 28.9 bits (63), Expect = 9.9
Identities = 20/81 (24%), Positives = 37/81 (44%), Gaps = 6/81 (7%)
Query: 17 CVFCGSSPGKKTTYQDAAMNLGNELVSRNIDLVYGGGSIGLMGLVSQAVHDGGRHVIGVI 76
C CGS G T A ++ + + + L G + Q + D +H++ +I
Sbjct: 186 CPTCGSCSGMFT-----ANSMNSLMEMLGMALPGNGTIVATSEARHQLIKDAAKHLMNLI 240
Query: 77 PKTLMPRE-LTGETVGEVKAV 96
K + PR+ +T ET+ + A+
Sbjct: 241 EKDIRPRDIITKETIDDAFAL 261
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.136 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,383,829
Number of Sequences: 164201
Number of extensions: 1175298
Number of successful extensions: 2897
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 2885
Number of HSP's gapped (non-prelim): 21
length of query: 220
length of database: 59,974,054
effective HSP length: 106
effective length of query: 114
effective length of database: 42,568,748
effective search space: 4852837272
effective search space used: 4852837272
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC148995.1