BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148763.8 - phase: 1 /pseudo
(180 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
APMA_MOUSE (Q9D7N9) Adipocyte plasma membrane-associated protein... 72 5e-13
APMA_HUMAN (Q9HDC9) Adipocyte plasma membrane-associated protein... 72 9e-13
STS1_ARATH (P94111) Strictosidine synthase 1 precursor (EC 4.3.3... 68 1e-11
STS3_ARATH (P92976) Strictosidine synthase 3 precursor (EC 4.3.3... 67 2e-11
STSY_RAUSE (P68175) Strictosidine synthase precursor (EC 4.3.3.2) 49 6e-06
STSY_RAUMA (P68174) Strictosidine synthase precursor (EC 4.3.3.2... 49 6e-06
STSY_CATRO (P18417) Strictosidine synthase precursor (EC 4.3.3.2) 49 8e-06
YFHM_ECOLI (P76578) Hypothetical lipoprotein yfhM precursor 35 0.072
PON2_CHICK (Q90952) Serum paraoxonase/arylesterase 2 (EC 3.1.1.2... 35 0.094
SNDH_ACELI (Q44091) L-sorbosone dehydrogenase (EC 1.1.1.-) (SNDH) 35 0.12
PON2_MELGA (Q91090) Serum paraoxonase/arylesterase 2 (EC 3.1.1.2... 35 0.12
POST_XENLA (P27536) Posterior protein 33 0.47
CYSI_SALTY (P17845) Sulfite reductase [NADPH] hemoprotein beta-c... 32 1.0
MUTL_RICCN (Q92FW6) DNA mismatch repair protein mutL 30 2.3
KCY_AERPE (Q9YDD5) Cytidylate kinase (EC 2.7.4.14) (CK) (Cytidin... 30 2.3
SPEA_SHIFL (Q83Q93) Biosynthetic arginine decarboxylase (EC 4.1.... 30 3.9
SPEA_SALTY (P60659) Biosynthetic arginine decarboxylase (EC 4.1.... 30 3.9
SPEA_SALTI (P60658) Biosynthetic arginine decarboxylase (EC 4.1.... 30 3.9
SPEA_ECOLI (P21170) Biosynthetic arginine decarboxylase (EC 4.1.... 30 3.9
SPEA_ECOL6 (Q8FE34) Biosynthetic arginine decarboxylase (EC 4.1.... 30 3.9
>APMA_MOUSE (Q9D7N9) Adipocyte plasma membrane-associated protein
(Protein DD16)
Length = 415
Score = 72.4 bits (176), Expect = 5e-13
Identities = 41/127 (32%), Positives = 65/127 (50%), Gaps = 1/127 (0%)
Query: 54 GRPLGLALEKTGELIVADAHLGLLRVTQKEGKEPKVEILANEHDGLKFKLTDGVDVGEDG 113
GRPLG+ G L V DA+ GL V ++ + +G K + + V DG
Sbjct: 149 GRPLGIRAGPNGTLFVVDAYKGLFEVNPQKRSVKLLLSSETPIEGKKMSFVNDLTVTRDG 208
Query: 114 T-IYFTEATYKYNLYDFYNDILEGEPHGRFMSYNPATKKVTLLARNLYFANRVAIAPDQK 172
IYFT+++ K+ D+ ++E GR + Y+ TK+V +L L F N V ++P++
Sbjct: 209 RKIYFTDSSSKWQRRDYLLLVMEATDDGRLLEYDTVTKEVKVLLDQLQFPNGVQLSPEED 268
Query: 173 FVVYCET 179
FV+ ET
Sbjct: 269 FVLVAET 275
>APMA_HUMAN (Q9HDC9) Adipocyte plasma membrane-associated protein
(BSCv protein)
Length = 416
Score = 71.6 bits (174), Expect = 9e-13
Identities = 45/129 (34%), Positives = 73/129 (55%), Gaps = 5/129 (3%)
Query: 54 GRPLGLALEKTGELIVADAHLGLLRVTQKEGKEPKVEILANEH--DGLKFKLTDGVDVGE 111
GRPLG+ G L VADA+ GL V + +E K+ +L++E +G + + V +
Sbjct: 150 GRPLGIRAGPNGTLFVADAYKGLFEVNPWK-REVKL-LLSSETPIEGKNMSFVNDLTVTQ 207
Query: 112 DGT-IYFTEATYKYNLYDFYNDILEGEPHGRFMSYNPATKKVTLLARNLYFANRVAIAPD 170
DG IYFT+++ K+ D+ ++EG GR + Y+ T++V +L L F N V ++P
Sbjct: 208 DGRKIYFTDSSSKWQRRDYLLLVMEGTDDGRLLEYDTVTREVKVLLDQLRFPNGVQLSPA 267
Query: 171 QKFVVYCET 179
+ FV+ ET
Sbjct: 268 EDFVLVAET 276
>STS1_ARATH (P94111) Strictosidine synthase 1 precursor (EC 4.3.3.2)
(SS-1)
Length = 335
Score = 67.8 bits (164), Expect = 1e-11
Identities = 48/155 (30%), Positives = 76/155 (48%), Gaps = 14/155 (9%)
Query: 32 EDGWIKRITVADSVVENWVHSS---------GRPLGLAL-EKTGELIVADAHLGLLRVTQ 81
E G++ + +S +W + GRP G+A EKTG+L VADA LGL ++
Sbjct: 66 ETGYVDFAQITESSNSSWCDGTIGTALAGRCGRPAGIAFNEKTGDLYVADAPLGLHVISP 125
Query: 82 KEGKEPKVEILANEHDGLKFKLTDGVDVGED-GTIYFTEATYKYNLYDFYNDILEGEPHG 140
G K+ + DG FK DG+DV G +YFT + +++ + + G
Sbjct: 126 AGGLATKI---TDSVDGKPFKFLDGLDVDPTTGVVYFTSFSSRFSPIQVLIALGLKDATG 182
Query: 141 RFMSYNPATKKVTLLARNLYFANRVAIAPDQKFVV 175
+ Y+P+TK VT+L L + A++ D FV+
Sbjct: 183 KLYKYDPSTKVVTVLMEGLSGSAGCAVSSDGSFVL 217
>STS3_ARATH (P92976) Strictosidine synthase 3 precursor (EC 4.3.3.2)
(SS-3)
Length = 329
Score = 67.0 bits (162), Expect = 2e-11
Identities = 44/128 (34%), Positives = 65/128 (50%), Gaps = 5/128 (3%)
Query: 50 VHSSGRPLGLALE-KTGELIVADAHLGLLRVTQKEGKEPKVEILANEHDGLKFKLTDGVD 108
V GRP G+A KTG+L VADA LGL + ++ G K+ A+ G F DG+D
Sbjct: 95 VEKCGRPAGIAFNTKTGDLYVADAALGLHVIPRRGGLAKKI---ADSVGGKPFLFLDGLD 151
Query: 109 VGED-GTIYFTEATYKYNLYDFYNDILEGEPHGRFMSYNPATKKVTLLARNLYFANRVAI 167
V G +YFT + + D + + G+F Y+P+ K VT+L L + A+
Sbjct: 152 VDPTTGVVYFTSFSSTFGPRDVLKAVATKDSTGKFFKYDPSKKVVTVLMEGLSGSAGCAV 211
Query: 168 APDQKFVV 175
+ D FV+
Sbjct: 212 SSDGSFVL 219
>STSY_RAUSE (P68175) Strictosidine synthase precursor (EC 4.3.3.2)
Length = 344
Score = 48.9 bits (115), Expect = 6e-06
Identities = 32/117 (27%), Positives = 55/117 (46%), Gaps = 4/117 (3%)
Query: 63 KTGELIVADAHLGLLRVTQKEGKEPKVEILANEHDGLKFKLTDGVDVGE-DGTIYFTEAT 121
+ +L + D + L V + G + LA DG+ FK V V + G +YFT+ +
Sbjct: 112 QNNQLYIVDCYYHLSVVGSEGGHATQ---LATSVDGVPFKWLYAVTVDQRTGIVYFTDVS 168
Query: 122 YKYNLYDFYNDILEGEPHGRFMSYNPATKKVTLLARNLYFANRVAIAPDQKFVVYCE 178
Y+ + + GR + Y+P+TK+ TLL + L+ ++ D FV+ E
Sbjct: 169 TLYDDRGVQQIMDTSDKTGRLIKYDPSTKETTLLLKELHVPGGAEVSADSSFVLVAE 225
>STSY_RAUMA (P68174) Strictosidine synthase precursor (EC 4.3.3.2)
(Fragment)
Length = 342
Score = 48.9 bits (115), Expect = 6e-06
Identities = 32/117 (27%), Positives = 55/117 (46%), Gaps = 4/117 (3%)
Query: 63 KTGELIVADAHLGLLRVTQKEGKEPKVEILANEHDGLKFKLTDGVDVGE-DGTIYFTEAT 121
+ +L + D + L V + G + LA DG+ FK V V + G +YFT+ +
Sbjct: 110 QNNQLYIVDCYYHLSVVGSEGGHATQ---LATSVDGVPFKWLYAVTVDQRTGIVYFTDVS 166
Query: 122 YKYNLYDFYNDILEGEPHGRFMSYNPATKKVTLLARNLYFANRVAIAPDQKFVVYCE 178
Y+ + + GR + Y+P+TK+ TLL + L+ ++ D FV+ E
Sbjct: 167 TLYDDRGVQQIMDTSDKTGRLIKYDPSTKETTLLLKELHVPGGAEVSADSSFVLVAE 223
>STSY_CATRO (P18417) Strictosidine synthase precursor (EC 4.3.3.2)
Length = 352
Score = 48.5 bits (114), Expect = 8e-06
Identities = 37/125 (29%), Positives = 57/125 (45%), Gaps = 18/125 (14%)
Query: 63 KTGELIVADAHLGLLRVTQKEGKEPKVEILANEHDGLKFKLTDGVDVGE-DGTIYFTEAT 121
K ++ + D H L V ++ G + LA G+ FK V V + G +YFT+ +
Sbjct: 116 KNSQMYIVDGHYHLCVVGKEGGYATQ---LATSVQGVPFKWLYAVTVDQRTGIVYFTDVS 172
Query: 122 YKYNLYDFYNDILEG--------EPHGRFMSYNPATKKVTLLARNLYFANRVAIAPDQKF 173
++D EG + GR M Y+P+TK+ TLL + L+ I+ D F
Sbjct: 173 ------SIHDDSPEGVEEIMNTSDRTGRLMKYDPSTKETTLLLKELHVPGGAEISADGSF 226
Query: 174 VVYCE 178
VV E
Sbjct: 227 VVVAE 231
>YFHM_ECOLI (P76578) Hypothetical lipoprotein yfhM precursor
Length = 1653
Score = 35.4 bits (80), Expect = 0.072
Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 2/84 (2%)
Query: 55 RPLGLALEKTGELIVADAHLGLLRVTQKEGKEPK-VEILANEHDGLKFKLTDGVDVGEDG 113
R L LALE ++ ++ + K G++PK V +L + D +TD V +
Sbjct: 857 RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYV-TPDPW 915
Query: 114 TIYFTEATYKYNLYDFYNDILEGE 137
+F + Y ++YD Y ++EG+
Sbjct: 916 QAFFGQKRYGADIYDIYGQVIEGQ 939
>PON2_CHICK (Q90952) Serum paraoxonase/arylesterase 2 (EC 3.1.1.2)
(EC 3.1.8.1) (PON 2) (Serum aryldialkylphosphatase 2)
(A-esterase 2) (Aromatic esterase 2)
Length = 354
Score = 35.0 bits (79), Expect = 0.094
Identities = 26/86 (30%), Positives = 42/86 (48%), Gaps = 4/86 (4%)
Query: 89 VEILANEHDGLKFKLTDGVDVGEDGTIYFTEATYKYNLYDFYNDILEGEPHGRFMSYNPA 148
V + HD L + D V VG D + Y T Y Y+ + ++ G + Y+P
Sbjct: 153 VHLKTIRHD-LLTSVNDVVAVGPD-SFYATNDHYFYDFILMFLEMYLGLTWSNVVYYSP- 209
Query: 149 TKKVTLLARNLYFANRVAIAPDQKFV 174
K+V +A Y AN + I+PD+K++
Sbjct: 210 -KEVKEVAAGFYSANGINISPDKKYI 234
>SNDH_ACELI (Q44091) L-sorbosone dehydrogenase (EC 1.1.1.-) (SNDH)
Length = 449
Score = 34.7 bits (78), Expect = 0.12
Identities = 14/21 (66%), Positives = 19/21 (89%)
Query: 51 HSSGRPLGLALEKTGELIVAD 71
H+ GRP+GLAL+K+G L+VAD
Sbjct: 412 HAHGRPVGLALDKSGALLVAD 432
>PON2_MELGA (Q91090) Serum paraoxonase/arylesterase 2 (EC 3.1.1.2)
(EC 3.1.8.1) (PON 2) (Serum aryldialkylphosphatase 2)
(A-esterase 2) (Aromatic esterase 2)
Length = 354
Score = 34.7 bits (78), Expect = 0.12
Identities = 25/79 (31%), Positives = 40/79 (49%), Gaps = 4/79 (5%)
Query: 96 HDGLKFKLTDGVDVGEDGTIYFTEATYKYNLYDFYNDILEGEPHGRFMSYNPATKKVTLL 155
HD L + D V VG D + Y T Y Y+ + ++ G + Y+P K+V +
Sbjct: 160 HD-LLTSVNDVVAVGPD-SFYATNDHYFYDFILMFLEMYLGLTWSNVVYYSP--KEVKEV 215
Query: 156 ARNLYFANRVAIAPDQKFV 174
A Y AN + I+PD+K++
Sbjct: 216 AAGFYSANGINISPDKKYI 234
>POST_XENLA (P27536) Posterior protein
Length = 555
Score = 32.7 bits (73), Expect = 0.47
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 1/70 (1%)
Query: 36 IKRITVADSVVENWVHSSGRPLGLALEKTGELIVADAHLGLLRVTQKEGKEPKVEILANE 95
+K +A++ E W + + LEK GE ++ A +++ E KVE+LA +
Sbjct: 64 LKMKVIAENKAEQWRNEKVQ-FREDLEKFGESLIVAASTSEEHISELEELREKVELLAKQ 122
Query: 96 HDGLKFKLTD 105
+D L+ KL D
Sbjct: 123 NDLLEEKLKD 132
>CYSI_SALTY (P17845) Sulfite reductase [NADPH] hemoprotein
beta-component (EC 1.8.1.2) (SIR-HP) (SIRHP)
Length = 569
Score = 31.6 bits (70), Expect = 1.0
Identities = 22/83 (26%), Positives = 38/83 (45%), Gaps = 17/83 (20%)
Query: 23 EEASYIYGCEDGWIKRITVADSVVENWVHSSGRPLGLALEKTGELIVADAHLGLLRVTQK 82
+ ++ G +D W + + + + ++ GRPL KTG L +A H G R+T
Sbjct: 343 DRIGWVKGIDDKWHLTLFIENGRILDY---PGRPL-----KTGLLEIAKIHQGEFRITAN 394
Query: 83 ---------EGKEPKVEILANEH 96
E ++ K+E LA +H
Sbjct: 395 QNLIIASVPESQKAKIETLARDH 417
>MUTL_RICCN (Q92FW6) DNA mismatch repair protein mutL
Length = 610
Score = 30.4 bits (67), Expect = 2.3
Identities = 22/76 (28%), Positives = 39/76 (50%), Gaps = 10/76 (13%)
Query: 40 TVADSVVENWVHSSGRPLGLALEKTGE--LIVADAHLGLLRVTQKEGKEPKVEILANEHD 97
+V +VEN V +S + + LE+ G+ +I++D +G+ T KE +EI H
Sbjct: 25 SVVKELVENAVDASSTKIDIILERAGKNLIIISDDGIGM---TDKE-----LEIAVERHT 76
Query: 98 GLKFKLTDGVDVGEDG 113
KF +D +++ G
Sbjct: 77 TSKFDESDFLNINTFG 92
>KCY_AERPE (Q9YDD5) Cytidylate kinase (EC 2.7.4.14) (CK) (Cytidine
monophosphate kinase) (CMP kinase)
Length = 186
Score = 30.4 bits (67), Expect = 2.3
Identities = 21/74 (28%), Positives = 36/74 (48%), Gaps = 4/74 (5%)
Query: 67 LIVADAHLGLLRVTQKEG---KEPKVEILANE-HDGLKFKLTDGVDVGEDGTIYFTEATY 122
L+ A + + R+ +++G + E + E L+FK G+DV + + T T
Sbjct: 107 LVKAPVWVRVRRIARRDGVPLRRALAETVEREWSQRLRFKRYYGIDVSDTTIFHLTVDTS 166
Query: 123 KYNLYDFYNDILEG 136
Y++ D Y ILEG
Sbjct: 167 MYSVEDTYRLILEG 180
>SPEA_SHIFL (Q83Q93) Biosynthetic arginine decarboxylase (EC
4.1.1.19) (ADC)
Length = 662
Score = 29.6 bits (65), Expect = 3.9
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Query: 45 VVENWVHSSGRPLGLALEKTGELIVADAHLGLLR 78
V+E+ +HS G PLGL EL+ AH G+ R
Sbjct: 134 VIESLIHS-GEPLGLEAGSKAELMAVLAHAGMTR 166
>SPEA_SALTY (P60659) Biosynthetic arginine decarboxylase (EC
4.1.1.19) (ADC)
Length = 658
Score = 29.6 bits (65), Expect = 3.9
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Query: 45 VVENWVHSSGRPLGLALEKTGELIVADAHLGLLR 78
V+E+ +HS G PLGL EL+ AH G+ R
Sbjct: 134 VIESLIHS-GEPLGLEAGSKAELMAVLAHAGMTR 166
>SPEA_SALTI (P60658) Biosynthetic arginine decarboxylase (EC
4.1.1.19) (ADC)
Length = 658
Score = 29.6 bits (65), Expect = 3.9
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Query: 45 VVENWVHSSGRPLGLALEKTGELIVADAHLGLLR 78
V+E+ +HS G PLGL EL+ AH G+ R
Sbjct: 134 VIESLIHS-GEPLGLEAGSKAELMAVLAHAGMTR 166
>SPEA_ECOLI (P21170) Biosynthetic arginine decarboxylase (EC
4.1.1.19) (ADC)
Length = 658
Score = 29.6 bits (65), Expect = 3.9
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Query: 45 VVENWVHSSGRPLGLALEKTGELIVADAHLGLLR 78
V+E+ +HS G PLGL EL+ AH G+ R
Sbjct: 134 VIESLIHS-GEPLGLEAGSKAELMAVLAHAGMTR 166
>SPEA_ECOL6 (Q8FE34) Biosynthetic arginine decarboxylase (EC
4.1.1.19) (ADC)
Length = 658
Score = 29.6 bits (65), Expect = 3.9
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Query: 45 VVENWVHSSGRPLGLALEKTGELIVADAHLGLLR 78
V+E+ +HS G PLGL EL+ AH G+ R
Sbjct: 134 VIESLIHS-GEPLGLEAGSKAELMAVLAHAGMTR 166
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.330 0.148 0.475
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,458,016
Number of Sequences: 164201
Number of extensions: 897045
Number of successful extensions: 2606
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 2583
Number of HSP's gapped (non-prelim): 35
length of query: 180
length of database: 59,974,054
effective HSP length: 103
effective length of query: 77
effective length of database: 43,061,351
effective search space: 3315724027
effective search space used: 3315724027
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC148763.8