BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148762.6 + phase: 0
(93 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SYS_UREPA (Q9PR38) Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--... 33 0.13
MACF_HUMAN (Q9UPN3) Microtubule-actin crosslinking factor 1, iso... 32 0.37
MAC4_HUMAN (Q96PK2) Microtubule-actin crosslinking factor 1, iso... 32 0.37
MYSC_CHICK (P29616) Myosin heavy chain, cardiac muscle isoform (... 31 0.62
MACF_MOUSE (Q9QXZ0) Microtubule-actin crosslinking factor 1 (Act... 31 0.62
ISPH_PHOPR (Q6LUK8) 4-hydroxy-3-methylbut-2-enyl diphosphate red... 31 0.62
ISPH_VIBVY (Q7MNM5) 4-hydroxy-3-methylbut-2-enyl diphosphate red... 30 0.81
ISPH_VIBVU (Q8DET0) 4-hydroxy-3-methylbut-2-enyl diphosphate red... 30 0.81
MYS_AEQIR (P24733) Myosin heavy chain, striated muscle 30 1.1
ID1_RAT (P41135) DNA-binding protein inhibitor ID-1 30 1.1
ID1_MOUSE (P20067) DNA-binding protein inhibitor ID-1 (ID) 30 1.1
ID1_HUMAN (P41134) DNA-binding protein inhibitor ID-1 (ID) 30 1.1
DE21_ANTMA (Q8RVL4) MADS box protein defh21 (DEFICIENS homolog 21) 30 1.1
NFL1_MOUSE (Q61985) Nuclear factor erythroid 2 related factor 1 ... 30 1.4
NFL1_HUMAN (Q14494) Nuclear factor erythroid 2 related factor 1 ... 30 1.4
RPOC_TROWT (Q820D6) DNA-directed RNA polymerase beta' chain (EC ... 29 1.8
RPOC_TROW8 (Q820D9) DNA-directed RNA polymerase beta' chain (EC ... 29 1.8
MYH6_RABIT (P04460) Myosin heavy chain, cardiac muscle alpha iso... 29 1.8
MUS2_BACHD (Q9K8A0) MutS2 protein 29 1.8
LMB_HIRME (Q25092) Laminin B-chain (Fragment) 29 1.8
>SYS_UREPA (Q9PR38) Seryl-tRNA synthetase (EC 6.1.1.11)
(Serine--tRNA ligase) (SerRS)
Length = 418
Score = 33.1 bits (74), Expect = 0.13
Identities = 21/64 (32%), Positives = 33/64 (50%), Gaps = 11/64 (17%)
Query: 19 DQIIDLVCKLRQLVPEIRNRRSDKVPASKV-----------LQETCNYIRNLQREVDDLS 67
DQI DL +R L+ + +N + K SK LQ+T + + N++ E+ D+S
Sbjct: 29 DQIFDLDVLVRSLMQQEQNLNAKKNQLSKEIGILAKNKDPKLQQTLDLVNNIKNELQDIS 88
Query: 68 LRLS 71
L LS
Sbjct: 89 LTLS 92
>MACF_HUMAN (Q9UPN3) Microtubule-actin crosslinking factor 1, isoforms
1/2/3 (Actin cross-linking family protein 7) (Macrophin
1) (Trabeculin-alpha) (620 kDa actin-binding protein)
(ABP620)
Length = 5430
Score = 31.6 bits (70), Expect = 0.37
Identities = 20/85 (23%), Positives = 44/85 (51%), Gaps = 2/85 (2%)
Query: 11 GGSTRISDDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLSLRL 70
G R +Q+++L +L + +R+S + Q+ ++ +L ++D ++
Sbjct: 2805 GEEKRTLQNQLVELKNHWEELSKKTADRQSRLKDCMQKAQKYQWHVEDLVPWIEDCKAKM 2864
Query: 71 SQLLATIDSDSAEASIIRS--LLNQ 93
S+L T+D E+S++RS +LN+
Sbjct: 2865 SELRVTLDPVQLESSLLRSKAMLNE 2889
>MAC4_HUMAN (Q96PK2) Microtubule-actin crosslinking factor 1, isoform
4
Length = 5938
Score = 31.6 bits (70), Expect = 0.37
Identities = 20/85 (23%), Positives = 44/85 (51%), Gaps = 2/85 (2%)
Query: 11 GGSTRISDDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLSLRL 70
G R +Q+++L +L + +R+S + Q+ ++ +L ++D ++
Sbjct: 3307 GEEKRTLQNQLVELKNHWEELSKKTADRQSRLKDCMQKAQKYQWHVEDLVPWIEDCKAKM 3366
Query: 71 SQLLATIDSDSAEASIIRS--LLNQ 93
S+L T+D E+S++RS +LN+
Sbjct: 3367 SELRVTLDPVQLESSLLRSKAMLNE 3391
>MYSC_CHICK (P29616) Myosin heavy chain, cardiac muscle isoform
(Fragment)
Length = 1102
Score = 30.8 bits (68), Expect = 0.62
Identities = 12/82 (14%), Positives = 43/82 (51%)
Query: 1 MSSRRSRQQSGGSTRISDDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQ 60
M R++++ G +++ + ++DL Q+ +++ + + + +++ + LQ
Sbjct: 212 MDLERAKRKLEGDLKLTQESVMDLENDKLQMEEKLKKKEFEMSQLNSKIEDEQAIVMQLQ 271
Query: 61 REVDDLSLRLSQLLATIDSDSA 82
+++ +L R+ +L ++++ A
Sbjct: 272 KKIKELQARIEELEEELEAERA 293
>MACF_MOUSE (Q9QXZ0) Microtubule-actin crosslinking factor 1 (Actin
cross-linking family 7)
Length = 5327
Score = 30.8 bits (68), Expect = 0.62
Identities = 19/85 (22%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Query: 11 GGSTRISDDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLSLRL 70
G + +Q+++L L + NR+S + Q+ ++ +L +D+ ++
Sbjct: 2804 GEEKKTLQNQLVELRSHWEDLSKKTANRQSRLKDCMQKAQKYQGHVEDLVPWIDECKSKM 2863
Query: 71 SQLLATIDSDSAEASIIRS--LLNQ 93
+L T+D E+S++RS +LN+
Sbjct: 2864 FELQVTLDPVQLESSLLRSKAMLNE 2888
>ISPH_PHOPR (Q6LUK8) 4-hydroxy-3-methylbut-2-enyl diphosphate
reductase (EC 1.17.1.2)
Length = 313
Score = 30.8 bits (68), Expect = 0.62
Identities = 18/77 (23%), Positives = 39/77 (50%), Gaps = 9/77 (11%)
Query: 14 TRISDDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLSLRLSQL 73
T +S D+ D++ +LR++ PEI+ R D + C +N Q V +++ + +
Sbjct: 167 TTLSVDETADVIDQLRKVFPEIQGPRKDDI---------CYATQNRQDAVREMAESVDVM 217
Query: 74 LATIDSDSAEASIIRSL 90
+ +S+ ++ +R L
Sbjct: 218 IVVGSKNSSNSNRLREL 234
>ISPH_VIBVY (Q7MNM5) 4-hydroxy-3-methylbut-2-enyl diphosphate
reductase (EC 1.17.1.2)
Length = 316
Score = 30.4 bits (67), Expect = 0.81
Identities = 16/51 (31%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Query: 14 TRISDDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVD 64
T +S D+ D++ +LR++ PEI+ R D + Q + +R L ++VD
Sbjct: 169 TTLSVDETADVIDELRRVFPEIQGPRKDDI--CYATQNRQDAVRELAKDVD 217
>ISPH_VIBVU (Q8DET0) 4-hydroxy-3-methylbut-2-enyl diphosphate
reductase (EC 1.17.1.2)
Length = 316
Score = 30.4 bits (67), Expect = 0.81
Identities = 16/51 (31%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Query: 14 TRISDDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVD 64
T +S D+ D++ +LR++ PEI+ R D + Q + +R L ++VD
Sbjct: 169 TTLSVDETADVIDELRRVFPEIQGPRKDDI--CYATQNRQDAVRELAKDVD 217
>MYS_AEQIR (P24733) Myosin heavy chain, striated muscle
Length = 1938
Score = 30.0 bits (66), Expect = 1.1
Identities = 17/78 (21%), Positives = 41/78 (51%), Gaps = 3/78 (3%)
Query: 3 SRRSRQQSGGSTRISDDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQRE 62
++R +Q ST+ + + DL R+L +R + ++ + L++ N + LQR+
Sbjct: 1048 AKRKVEQDLKSTQ---ENVEDLERVKRELEENVRRKEAEISSLNSKLEDEQNLVSQLQRK 1104
Query: 63 VDDLSLRLSQLLATIDSD 80
+ +L R+ +L ++++
Sbjct: 1105 IKELQARIEELEEELEAE 1122
>ID1_RAT (P41135) DNA-binding protein inhibitor ID-1
Length = 164
Score = 30.0 bits (66), Expect = 1.1
Identities = 16/41 (39%), Positives = 26/41 (63%), Gaps = 2/41 (4%)
Query: 27 KLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLS 67
+L++LVP + R KV ++LQ +YIR+LQ E++ S
Sbjct: 68 RLKELVPTLPQNR--KVSKVEILQHVIDYIRDLQLELNSES 106
>ID1_MOUSE (P20067) DNA-binding protein inhibitor ID-1 (ID)
Length = 168
Score = 30.0 bits (66), Expect = 1.1
Identities = 16/41 (39%), Positives = 26/41 (63%), Gaps = 2/41 (4%)
Query: 27 KLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLS 67
+L++LVP + R KV ++LQ +YIR+LQ E++ S
Sbjct: 68 RLKELVPTLPQNR--KVSKVEILQHVIDYIRDLQLELNSES 106
>ID1_HUMAN (P41134) DNA-binding protein inhibitor ID-1 (ID)
Length = 155
Score = 30.0 bits (66), Expect = 1.1
Identities = 16/41 (39%), Positives = 26/41 (63%), Gaps = 2/41 (4%)
Query: 27 KLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLS 67
+L++LVP + R KV ++LQ +YIR+LQ E++ S
Sbjct: 75 RLKELVPTLPQNR--KVSKVEILQHVIDYIRDLQLELNSES 113
>DE21_ANTMA (Q8RVL4) MADS box protein defh21 (DEFICIENS homolog 21)
Length = 247
Score = 30.0 bits (66), Expect = 1.1
Identities = 21/56 (37%), Positives = 29/56 (51%), Gaps = 8/56 (14%)
Query: 20 QIIDLVCKLRQLVPEIRNRRSDKVPASKVL-------QETCNYIRNLQR-EVDDLS 67
QIID K + ++PE+ NR +V+ +ET N NLQR + DDLS
Sbjct: 66 QIIDRYVKAKGILPEMENRAGPHADNDQVIKELTRMKEETLNLQLNLQRYKGDDLS 121
>NFL1_MOUSE (Q61985) Nuclear factor erythroid 2 related factor 1
(NF-E2 related factor 1) (NFE2-related factor 1)
(Nuclear factor, erythroid derived 2, like 1)
Length = 741
Score = 29.6 bits (65), Expect = 1.4
Identities = 15/44 (34%), Positives = 26/44 (59%)
Query: 31 LVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLSLRLSQLL 74
L+ +IR R +K+ A + + I NL+R+V+DL ++LL
Sbjct: 623 LIRDIRRRGKNKMAAQNCRKRKLDTILNLERDVEDLQRDKARLL 666
>NFL1_HUMAN (Q14494) Nuclear factor erythroid 2 related factor 1
(NF-E2 related factor 1) (NFE2-related factor 1)
(Nuclear factor, erythroid derived 2, like 1)
(Transcription factor 11) (Transcription factor HBZ17)
(Transcription factor LCR-F1) (Locus con
Length = 772
Score = 29.6 bits (65), Expect = 1.4
Identities = 15/44 (34%), Positives = 26/44 (59%)
Query: 31 LVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLSLRLSQLL 74
L+ +IR R +K+ A + + I NL+R+V+DL ++LL
Sbjct: 654 LIRDIRRRGKNKMAAQNCRKRKLDTILNLERDVEDLQRDKARLL 697
>RPOC_TROWT (Q820D6) DNA-directed RNA polymerase beta' chain (EC
2.7.7.6) (RNAP beta' subunit) (Transcriptase beta'
chain) (RNA polymerase beta' subunit)
Length = 1286
Score = 29.3 bits (64), Expect = 1.8
Identities = 14/48 (29%), Positives = 26/48 (54%)
Query: 18 DDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDD 65
D I V +L + + E+++ ++ +KV E N + N++RE DD
Sbjct: 165 DASIASAVSELERTITELQDAKTSASRINKVRSEAENKMANIRREYDD 212
>RPOC_TROW8 (Q820D9) DNA-directed RNA polymerase beta' chain (EC
2.7.7.6) (RNAP beta' subunit) (Transcriptase beta'
chain) (RNA polymerase beta' subunit)
Length = 1286
Score = 29.3 bits (64), Expect = 1.8
Identities = 14/48 (29%), Positives = 26/48 (54%)
Query: 18 DDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDD 65
D I V +L + + E+++ ++ +KV E N + N++RE DD
Sbjct: 165 DASIASAVSELERTITELQDAKTSASRINKVRSEAENKMANIRREYDD 212
>MYH6_RABIT (P04460) Myosin heavy chain, cardiac muscle alpha
isoform (MyHC-alpha) (Alpha isomyosin) (Fragment)
Length = 465
Score = 29.3 bits (64), Expect = 1.8
Identities = 14/90 (15%), Positives = 44/90 (48%)
Query: 1 MSSRRSRQQSGGSTRISDDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQ 60
M R++++ G +++ + I+DL +QL ++ + D + +++ + LQ
Sbjct: 243 MDLERAKRKLEGDLKLTQESIMDLENDKQQLEERLKKKEFDISQLNSKIEDEQALVLQLQ 302
Query: 61 REVDDLSLRLSQLLATIDSDSAEASIIRSL 90
+++ + R+ +L ++++ + + L
Sbjct: 303 KKLKENQARIEELEEELEAERTARAKVEKL 332
>MUS2_BACHD (Q9K8A0) MutS2 protein
Length = 785
Score = 29.3 bits (64), Expect = 1.8
Identities = 18/65 (27%), Positives = 32/65 (48%), Gaps = 3/65 (4%)
Query: 20 QIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLSLRLSQLLATIDS 79
Q+ ++ L Q ++ SD A K LQE +LQ+++DDL ++LA +
Sbjct: 518 QVESMIASLEQ---SQKSAESDWEEAEKALQEAEQLRLDLQKKLDDLEKEKERILAEAEQ 574
Query: 80 DSAEA 84
+ +A
Sbjct: 575 QAEQA 579
>LMB_HIRME (Q25092) Laminin B-chain (Fragment)
Length = 400
Score = 29.3 bits (64), Expect = 1.8
Identities = 23/80 (28%), Positives = 37/80 (45%), Gaps = 6/80 (7%)
Query: 18 DDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQREVDDLSLRLSQL---- 73
D ++ +L KL+ L P + + S AS + E + + NLQ E + S + +L
Sbjct: 170 DQELDNLSSKLKDLKPNLASHGSRTGEASVKISELYSRVLNLQSEFNKSSAKTKELRENA 229
Query: 74 --LATIDSDSAEASIIRSLL 91
+ D A ASI SL+
Sbjct: 230 LEIQEQDVSGAYASIQESLV 249
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.130 0.339
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,434,528
Number of Sequences: 164201
Number of extensions: 260801
Number of successful extensions: 1174
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 26
Number of HSP's that attempted gapping in prelim test: 1135
Number of HSP's gapped (non-prelim): 65
length of query: 93
length of database: 59,974,054
effective HSP length: 69
effective length of query: 24
effective length of database: 48,644,185
effective search space: 1167460440
effective search space used: 1167460440
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148762.6