BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148755.1 + phase: 0
(190 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AMYH_YEAST (P08640) Glucoamylase S1/S2 precursor (EC 3.2.1.3) (G... 36 0.047
SGV1_YEAST (P23293) Serine/threonine-protein kinase SGV1 (EC 2.7... 35 0.10
NFM_HUMAN (P07197) Neurofilament triplet M protein (160 kDa neur... 34 0.18
DS_DROME (Q24292) Dachsous protein precursor (Adherin) 34 0.18
CYAA_NEUCR (Q01631) Adenylate cyclase (EC 4.6.1.1) (ATP pyrophos... 34 0.18
ANC2_HUMAN (Q9UJX6) Anaphase promoting complex subunit 2 (APC2) ... 34 0.18
AF9_HUMAN (P42568) AF-9 protein 34 0.23
FMN2_MOUSE (Q9JL04) Formin 2 33 0.40
DLG5_HUMAN (Q8TDM6) Discs, large homolog 5 (Placenta and prostat... 33 0.40
UN89_CAEEL (O01761) Muscle M-line assembly protein unc-89 (Uncoo... 33 0.52
N124_SCHPO (Q09904) Nucleoporin nup124 (Nuclear pore protein nup... 33 0.52
LP2A_MOUSE (Q61033) Lamina-associated polypeptide 2 isoforms alp... 33 0.52
GAR2_SCHPO (P41891) Protein gar2 33 0.52
PTP3_DICDI (P54637) Protein-tyrosine phosphatase 3 (EC 3.1.3.48)... 32 0.68
CORO_YEAST (Q06440) Coronin-like protein 32 0.68
SMA1_HUMAN (Q15797) Mothers against decapentaplegic homolog 1 (S... 32 0.88
YB54_XENLA (P45441) Cytoplasmic RNA-binding protein P54 (Y-box f... 32 1.2
SMA1_COTJA (Q9I962) Mothers against decapentaplegic homolog 1 (S... 32 1.2
RAD4_SCHPO (P32372) S-M checkpoint control protein rad4 (Protein... 32 1.2
MCM3_YEAST (P24279) DNA replication licensing factor MCM3 (Minic... 32 1.2
>AMYH_YEAST (P08640) Glucoamylase S1/S2 precursor (EC 3.2.1.3)
(Glucan 1,4-alpha-glucosidase) (1,4-alpha-D-glucan
glucohydrolase)
Length = 1367
Score = 36.2 bits (82), Expect = 0.047
Identities = 30/116 (25%), Positives = 45/116 (37%), Gaps = 2/116 (1%)
Query: 9 STTGDCKPLTTFVHTNTGAFREVVQRLTGPSEGNATKEEQTAKVAP-ITKRTP-PKLHER 66
+T+ + TT T+ + P+ + TKE+ T TK P P H+
Sbjct: 233 TTSSTSESSTTTSSTSESSTSSSTTAPATPTTTSCTKEKPTPPTTTSCTKEKPTPPHHDT 292
Query: 67 RKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTTPST 122
C K K K + P +PSS + SS P S ++ +P T ST
Sbjct: 293 TPCTKKKTTTSKTCTKKTTTPVPTPSSSTTESSSAPVPTPSSSTTESSSAPVTSST 348
>SGV1_YEAST (P23293) Serine/threonine-protein kinase SGV1 (EC
2.7.1.37)
Length = 657
Score = 35.0 bits (79), Expect = 0.10
Identities = 27/93 (29%), Positives = 41/93 (44%), Gaps = 4/93 (4%)
Query: 62 KLHERRKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSLLPS----P 117
+ H R KP+ + +Y +Q +P +K+ N S S ++G PS P
Sbjct: 554 RYHNPRYVNKPETNFNRQPQKYSRQESNAPINKNYNPSNGSRNMAGDHHQGSRPSHPQFP 613
Query: 118 TTPSTLFSRLTIMEDEKKQGSVINEINIEEEEK 150
+PS +LT EKK GS +E +E K
Sbjct: 614 ISPSQGQHQLTSKPIEKKNGSFKDERAKPDESK 646
>NFM_HUMAN (P07197) Neurofilament triplet M protein (160 kDa
neurofilament protein) (Neurofilament medium
polypeptide) (NF-M) (Neurofilament 3)
Length = 915
Score = 34.3 bits (77), Expect = 0.18
Identities = 40/124 (32%), Positives = 52/124 (41%), Gaps = 12/124 (9%)
Query: 39 SEGNATKEEQTAKVA---PITKRTP-PK--LHERRKCMKPKLEIVKPNLQYHKQP-GASP 91
SE ATKEE A P ++P PK + E+ K PK P + K P SP
Sbjct: 591 SEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPK----SPVEEKGKSPVPKSP 646
Query: 92 SSKSRNSSFPSSPVSGSGSSSLLPSPTTPSTLFS-RLTIMEDEKKQGSVINEINIEEEEK 150
+ S P SPV G S + SP + +E+ K + V EEEEK
Sbjct: 647 VEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEK 706
Query: 151 AIKE 154
+KE
Sbjct: 707 EVKE 710
>DS_DROME (Q24292) Dachsous protein precursor (Adherin)
Length = 3503
Score = 34.3 bits (77), Expect = 0.18
Identities = 20/42 (47%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
Query: 81 LQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTTPST 122
L H GAS S+KS++SS SS +G+GS L P P+ P T
Sbjct: 3360 LSEHSGSGASSSAKSKHSSSHSS--AGAGSVVLKPPPSAPPT 3399
>CYAA_NEUCR (Q01631) Adenylate cyclase (EC 4.6.1.1) (ATP
pyrophosphate-lyase) (Adenylyl cyclase)
Length = 2300
Score = 34.3 bits (77), Expect = 0.18
Identities = 19/55 (34%), Positives = 27/55 (48%), Gaps = 3/55 (5%)
Query: 67 RKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTTPS 121
+ C +P+L + N Q + P S ++ P VS +G S L SPTTPS
Sbjct: 115 QSCSQPQLSL---NSQQSQSPSQYHSQQTPTQQQPQQQVSPTGGSRLTQSPTTPS 166
>ANC2_HUMAN (Q9UJX6) Anaphase promoting complex subunit 2 (APC2)
(Cyclosome subunit 2)
Length = 822
Score = 34.3 bits (77), Expect = 0.18
Identities = 29/100 (29%), Positives = 44/100 (44%), Gaps = 6/100 (6%)
Query: 14 CKPLTTFVHTNTGAFREVVQRLTGPSEGNATKEEQTAKVAPITKRTPPKLHERRKCMKPK 73
C+P+ ++ T R++V LTG S+G + +K P + T + +P+
Sbjct: 421 CEPIRRYLRTREDTVRQIVAGLTGDSDGTGDLAVELSKTDPASLETGQDSED--DSGEPE 478
Query: 74 LEIVKPNLQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSL 113
+ P PG S SSK R+S S VS GS L
Sbjct: 479 DWVPDP---VDADPGKS-SSKRRSSDIISLLVSIYGSKDL 514
>AF9_HUMAN (P42568) AF-9 protein
Length = 568
Score = 33.9 bits (76), Expect = 0.23
Identities = 32/108 (29%), Positives = 43/108 (39%), Gaps = 18/108 (16%)
Query: 66 RRKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTTPSTLFS 125
RRK +K + PN H +S SS S +SS SS S S SSS S ++ S+ S
Sbjct: 132 RRKLLKAGGD---PNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 188
Query: 126 RLTIMEDEKKQGSVINEINIEEEEKAIKERRFYLHPSPRSKASGFNEP 173
T + + K +KE + R S F EP
Sbjct: 189 SST---------------SFSKPHKLMKEHKEKPSKDSREHKSAFKEP 221
>FMN2_MOUSE (Q9JL04) Formin 2
Length = 1567
Score = 33.1 bits (74), Expect = 0.40
Identities = 15/34 (44%), Positives = 19/34 (55%)
Query: 91 PSSKSRNSSFPSSPVSGSGSSSLLPSPTTPSTLF 124
P K+ P SP + SG S+LL SP+ P T F
Sbjct: 735 PPPKAPPEGLPGSPAAASGESALLTSPSGPQTKF 768
>DLG5_HUMAN (Q8TDM6) Discs, large homolog 5 (Placenta and prostate
DLG) (Discs large protein P-dlg)
Length = 1809
Score = 33.1 bits (74), Expect = 0.40
Identities = 27/76 (35%), Positives = 38/76 (49%), Gaps = 8/76 (10%)
Query: 51 KVAPITKRTPPKLHERRKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSS---FPSSPVSG 107
K+AP+ P + E +KC+ E+ P LQ + SP SR+S+ +PS P G
Sbjct: 1012 KLAPVV--IPAQFLEEQKCVPASGEL-SPELQ--EWAPYSPGHSSRHSNPPLYPSRPSVG 1066
Query: 108 SGSSSLLPSPTTPSTL 123
+ SL PS T S L
Sbjct: 1067 TVPRSLTPSTTVSSIL 1082
>UN89_CAEEL (O01761) Muscle M-line assembly protein unc-89
(Uncoordinated protein 89)
Length = 6632
Score = 32.7 bits (73), Expect = 0.52
Identities = 32/147 (21%), Positives = 57/147 (38%), Gaps = 6/147 (4%)
Query: 44 TKEEQTAKVAPITKRTPPKLHERRKCMKPKLEIVKPNLQYH-KQPGASPSSKSRNSSFPS 102
T E + ++ + ++TP K+ E+ K K + + ++ K P S + P+
Sbjct: 1516 THESEESRTSVKKEKTPEKVDEKPKSPTKKDKSPEKSITEEIKSPVKKEKSPEKVEEKPA 1575
Query: 103 SPVSGSGSSSLLPSPTTPSTLFSRLTIMEDEKKQGSVINEINIEEEEKAIKERRFYLHPS 162
SP S SPT S + +++ + SV+ E+ +E+ K
Sbjct: 1576 SPTKKEKSPEKPASPTKKSENEVKSPTKKEKSPEKSVVEELKSPKEKSPEK-----ADDK 1630
Query: 163 PRSKASGFNEPELLNLFPLASPKACEK 189
P+S PE + SP EK
Sbjct: 1631 PKSPTKKEKSPEKSATEDVKSPTKKEK 1657
>N124_SCHPO (Q09904) Nucleoporin nup124 (Nuclear pore protein
nup124)
Length = 1159
Score = 32.7 bits (73), Expect = 0.52
Identities = 25/78 (32%), Positives = 39/78 (49%), Gaps = 6/78 (7%)
Query: 92 SSKSRNSSFPSSPVS---GSGSSSLLPSPTTPSTLFSRLTIMEDEKKQG---SVINEINI 145
S KS NS PSS +S GS S + +P + ++L + L + DE+K S + + I
Sbjct: 135 SLKSLNSLHPSSALSKKLGSTSQHQIATPKSSASLLNILRSLHDEQKNTLNISSVKQDRI 194
Query: 146 EEEEKAIKERRFYLHPSP 163
E ++R+ PSP
Sbjct: 195 TEANPTCEKRKPSRSPSP 212
>LP2A_MOUSE (Q61033) Lamina-associated polypeptide 2 isoforms
alpha/zeta (Thymopoietin isoforms alpha/zeta) (TP
alpha/zeta)
Length = 692
Score = 32.7 bits (73), Expect = 0.52
Identities = 33/119 (27%), Positives = 47/119 (38%), Gaps = 14/119 (11%)
Query: 13 DCKPLTTFVHTNTGAFREVV---QRLTGPSEGNATKEEQTAKVAPITKRTPPKL------ 63
DC P + T +GAF + + + T P G E Q AK TKR PP+
Sbjct: 200 DCVPFSELASTPSGAFFQGISFPEISTRPPLGRT--ELQAAKKVQTTKRDPPRETCTDTA 257
Query: 64 ---HERRKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTT 119
R + P + P+ + + SS S F + VS + S SL+ TT
Sbjct: 258 LPGRGRPHKLAPGRSLFIPSELCYDRCVEKSSSPSSQREFAARLVSAAASPSLIRETTT 316
>GAR2_SCHPO (P41891) Protein gar2
Length = 500
Score = 32.7 bits (73), Expect = 0.52
Identities = 36/153 (23%), Positives = 64/153 (41%), Gaps = 16/153 (10%)
Query: 38 PSEGNATKEEQTAKVAPITKRTP------PKLHERRKCMKPKLEIVKPNLQYHKQPGASP 91
PS+ +E ++A + +T K +R +P + VK + K+ +S
Sbjct: 26 PSKSKKITKEAAKEIAKQSSKTDVSPKKSKKEAKRASSPEPSKKSVKKQKKSKKKEESSS 85
Query: 92 SSKSRNSSFPSSPVSGSGSSSLLPSPTTPSTLFS-----RLTIMEDEKKQGSVINEINIE 146
S+S +SS S S SS S ++ S S + + +EKK+ S + + E
Sbjct: 86 ESESESSSSESESSSSESESSSSESESSSSESSSSESEEEVIVKTEEKKESSSESSSSSE 145
Query: 147 EEEK-----AIKERRFYLHPSPRSKASGFNEPE 174
EE+ I+E++ S +S +E E
Sbjct: 146 SEEEEEAVVKIEEKKESSSDSSSESSSSESESE 178
>PTP3_DICDI (P54637) Protein-tyrosine phosphatase 3 (EC 3.1.3.48)
(Protein-tyrosine-phosphate phosphohydrolase 3)
Length = 989
Score = 32.3 bits (72), Expect = 0.68
Identities = 28/83 (33%), Positives = 38/83 (45%), Gaps = 3/83 (3%)
Query: 86 QPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTTPSTLFSRLTIMEDEKKQGSVINEINI 145
QP ASP S S +S F +S +S SGS S S T+ ST S + ++++
Sbjct: 359 QPNASPFSFSSSSLFSNSSLSNSGSGS--ASTTSTSTSSSNSMSSSPPPSLKTSFSQLD- 415
Query: 146 EEEEKAIKERRFYLHPSPRSKAS 168
E+ EK E P SK S
Sbjct: 416 EDREKMRLEFEMIKKPEMASKKS 438
>CORO_YEAST (Q06440) Coronin-like protein
Length = 651
Score = 32.3 bits (72), Expect = 0.68
Identities = 27/107 (25%), Positives = 44/107 (40%), Gaps = 5/107 (4%)
Query: 32 VQRLTGPSEGNATKEEQTAKVAPITKRTPPKLHERRKCMKPKLEIVKPNLQYHKQPGASP 91
V PS + EE + API TP E +K PK+E P+ + +P +
Sbjct: 490 VNNAEDPSRDTSGWEEADDEPAPIKIETPVTPTETKKDRTPKVE---PSKELKPEPVSIA 546
Query: 92 SSKSRNSSFPSSPVSGSGSSS--LLPSPTTPSTLFSRLTIMEDEKKQ 136
+ + + S P S + S S T PS++ + T + K+
Sbjct: 547 TDRKQEQSLPQEEKSSEKTKSPEQEKSATPPSSITAAKTAITASSKE 593
>SMA1_HUMAN (Q15797) Mothers against decapentaplegic homolog 1 (SMAD
1) (Mothers against DPP homolog 1) (Mad-related protein
1) (Transforming growth factor-beta signaling protein-1)
(BSP-1) (hSMAD1) (JV4-1)
Length = 465
Score = 32.0 bits (71), Expect = 0.88
Identities = 17/35 (48%), Positives = 23/35 (65%), Gaps = 2/35 (5%)
Query: 85 KQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTT 119
+QP + P S NSS+P+SP GS SS+ SPT+
Sbjct: 177 QQPNSHPFPHSPNSSYPNSP--GSSSSTYPHSPTS 209
>YB54_XENLA (P45441) Cytoplasmic RNA-binding protein P54 (Y-box
factor homolog) (MRNP3)
Length = 324
Score = 31.6 bits (70), Expect = 1.2
Identities = 21/81 (25%), Positives = 35/81 (42%), Gaps = 5/81 (6%)
Query: 46 EEQTAKVAPITKRTPPKLHERRKCMKPKLEIVKPNLQYHKQPGASPSSKSRNSSFPSSPV 105
EE + + P +R PP + RR P+ PN Q ++ + S++++ + P+S
Sbjct: 160 EETSPQQRPQRRRPPPFFYRRRFRRGPR-----PNNQQNQGAEVTDQSENKDPAAPTSEA 214
Query: 106 SGSGSSSLLPSPTTPSTLFSR 126
SG P P F R
Sbjct: 215 LASGDGQQRPPPRRFQQRFRR 235
>SMA1_COTJA (Q9I962) Mothers against decapentaplegic homolog 1 (SMAD
1) (Mothers against DPP homolog 1) (Mad-related protein
1)
Length = 465
Score = 31.6 bits (70), Expect = 1.2
Identities = 15/40 (37%), Positives = 23/40 (57%)
Query: 85 KQPGASPSSKSRNSSFPSSPVSGSGSSSLLPSPTTPSTLF 124
+QP + P S NSS+P+SP S S + P+ + P + F
Sbjct: 177 QQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPASSDPGSPF 216
>RAD4_SCHPO (P32372) S-M checkpoint control protein rad4 (Protein
cut5) (P74)
Length = 648
Score = 31.6 bits (70), Expect = 1.2
Identities = 20/63 (31%), Positives = 29/63 (45%), Gaps = 8/63 (12%)
Query: 106 SGSGSSSLLPSPTTPSTLFSRLTIMEDEKKQGSVINEINIEEEEKAIKERRFYLHPSPRS 165
S S +S+L TP T+ ++ + E+NIE E K K FYL+ P +
Sbjct: 265 SRSEENSVLLDDFTPETV--------QPLEENELDTELNIENEAKLFKNLTFYLYEFPNT 316
Query: 166 KAS 168
K S
Sbjct: 317 KVS 319
>MCM3_YEAST (P24279) DNA replication licensing factor MCM3
(Minichromosome maintenance protein 3)
Length = 971
Score = 31.6 bits (70), Expect = 1.2
Identities = 36/136 (26%), Positives = 58/136 (42%), Gaps = 27/136 (19%)
Query: 35 LTGPSEGNATKEEQTAKVAPITKRTPPKLHERRKCMKPKLEIVKPNLQYHKQPGASPSSK 94
L G GN EE++ ++KR+P K ++R+ ++ P+S
Sbjct: 737 LLGEDIGNDIDEEESEYEEALSKRSPQKSPKKRQRVR------------------QPASN 778
Query: 95 SRNSSFPSSPVSGSGSSSLLPSPTTPSTLFSRLTIMEDEKKQGSVINEI--NIEEEEKAI 152
S S S+P + SS TPS+ L +DE+ G N+I + +E+A
Sbjct: 779 S-GSPIKSTPRRSTASS----VNATPSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAE 833
Query: 153 KERRFY--LHPSPRSK 166
+RR L SPR +
Sbjct: 834 LQRRLQLGLRVSPRRR 849
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.309 0.127 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,662,928
Number of Sequences: 164201
Number of extensions: 1058802
Number of successful extensions: 4177
Number of sequences better than 10.0: 129
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 95
Number of HSP's that attempted gapping in prelim test: 4040
Number of HSP's gapped (non-prelim): 213
length of query: 190
length of database: 59,974,054
effective HSP length: 104
effective length of query: 86
effective length of database: 42,897,150
effective search space: 3689154900
effective search space used: 3689154900
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC148755.1