BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148718.8 - phase: 0
(755 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LCF3_HUMAN (O95573) Long-chain-fatty-acid--CoA ligase 3 (EC 6.2.... 602 e-171
LCF4_RAT (O35547) Long-chain-fatty-acid--CoA ligase 4 (EC 6.2.1.... 598 e-170
LCF4_HUMAN (O60488) Long-chain-fatty-acid--CoA ligase 4 (EC 6.2.... 594 e-169
LCF3_MOUSE (Q9CZW4) Long-chain-fatty-acid--CoA ligase 3 (EC 6.2.... 592 e-168
LCF4_MOUSE (Q9QUJ7) Long-chain-fatty-acid--CoA ligase 4 (EC 6.2.... 590 e-168
LCF3_RAT (Q63151) Long-chain-fatty-acid--CoA ligase 3 (EC 6.2.1.... 589 e-167
LCF1_YEAST (P30624) Long-chain-fatty-acid--CoA ligase 1 (EC 6.2.... 326 1e-88
LCF2_MOUSE (P41216) Long-chain-fatty-acid--CoA ligase 2 (EC 6.2.... 324 6e-88
LCF1_CAVPO (Q9JID6) Long-chain-fatty-acid--CoA ligase 1 (EC 6.2.... 316 2e-85
LCF2_RAT (P18163) Long-chain-fatty-acid--CoA ligase, liver isozy... 308 4e-83
LCF6_RAT (P33124) Long-chain-fatty-acid--CoA ligase 6 (EC 6.2.1.... 307 6e-83
LCF1_HUMAN (P33121) Long-chain-fatty-acid--CoA ligase 1 (EC 6.2.... 305 2e-82
LCF6_MOUSE (Q91WC3) Long-chain-fatty-acid--CoA ligase 6 (EC 6.2.... 305 3e-82
LCF6_HUMAN (Q9UKU0) Long-chain-fatty-acid--CoA ligase 6 (EC 6.2.... 304 5e-82
LCF5_HUMAN (Q9ULC5) Long-chain-fatty-acid--CoA ligase 5 (EC 6.2.... 304 5e-82
LCF5_RAT (O88813) Long-chain-fatty-acid--CoA ligase 5 (EC 6.2.1.... 303 8e-82
LCF4_YEAST (P47912) Long-chain-fatty-acid--CoA ligase 4 (EC 6.2.... 301 3e-81
LCF5_MOUSE (Q8JZR0) Long-chain-fatty-acid--CoA ligase 5 (EC 6.2.... 298 4e-80
LCF3_YEAST (P39002) Long-chain-fatty-acid--CoA ligase 3 (EC 6.2.... 294 5e-79
LCF2_YEAST (P39518) Long-chain-fatty-acid--CoA ligase 2 (EC 6.2.... 216 2e-55
>LCF3_HUMAN (O95573) Long-chain-fatty-acid--CoA ligase 3 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 3) (LACS 3)
Length = 720
Score = 602 bits (1551), Expect = e-171
Identities = 317/675 (46%), Positives = 438/675 (63%), Gaps = 16/675 (2%)
Query: 40 VPVLFSVFFLGKKKGKVRGVPVEVSGESGYAVRNARYSELVEVPWKGAPTMAHLFEPSCK 99
+P F +K +++ PV +S Y N+ L V + G T+ +F +
Sbjct: 39 IPFYFFSESRQEKSNRIKAKPVNSKPDSAYRSVNS-LDGLASVLYPGCDTLDKVFTYAKN 97
Query: 100 KHTHNRFLGTRKLIGKEFVTSSDGRKFEKVHLGEYEWETYGEVFARVSNFASGLLKLGHD 159
K + R LGTR+++ +E +G+ F+KV LG+Y W +Y +VF R NF +GL LG
Sbjct: 98 KFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQMLGQK 157
Query: 160 IDSHVAIFSDTRAEWFIALQGCFRQNITVVTIYASLGEDALIHSLNETQVSTLICDVKLL 219
+++AIF +TRAEW IA Q CF N +VT+YA+LG A++H+LNET+V+ +I +LL
Sbjct: 158 PKTNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELL 217
Query: 220 N-KLDAIRSKLTSLQNIIYFEDDSKEEHTFSEGLSSNCKIASFDEVEKLGKESPVEP--- 275
KL I S + L++II + F +G+ + A VE LG ++ +E
Sbjct: 218 QTKLKDIVSLVPRLRHIITVDGKPPTWSDFPKGIIVHTMAA----VEALGAKASMENQPH 273
Query: 276 SLPSKNAVAVVMYTSGSTGLPKGVMITHGNIVATTAAVMTVIPNLGSKDVYLAYLPLAHV 335
S P + +AV+MYTSGSTGLPKGVMI+H NI+A + IP LG +DVY+ YLPLAHV
Sbjct: 274 SKPLPSDIAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEEDVYIGYLPLAHV 333
Query: 336 FEMAAESVMLAAGVAIGYGSPMTLTDTSNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDG 395
E++AE V L+ G IGY SP TL D S+K+KKG+KGD ++LKPTL+ AVP I+DRI
Sbjct: 334 LELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPEIMDRIYKN 393
Query: 396 VVKKVEEKGGLAKNLFQIAYKRRLAAVKGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRF 455
V+ KV E +NLF +AY ++ + G + D+ +F+K+R++LGGN+R
Sbjct: 394 VMNKVSEMSSFQRNLFILAYNYKMEQISK------GRNTPLCDSFVFRKVRSLLGGNIRL 447
Query: 456 MLCGGAPLSGDSQQFINICVGAPIGQGYGLTETFAGAAFSEADDYSVGRVGPPLPCCYIK 515
+LCGGAPLS +Q+F+NIC P+GQGYGLTE+ SE DY+ GRVG PL CC IK
Sbjct: 448 LLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTGRVGAPLVCCEIK 507
Query: 516 LVSWEEGGYLSSDKPMPRGEVVVGGFSVTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGR 575
L +WEEGGY ++DKP PRGE+++GG SVT GY+KN+ KT F DE G RW TGDIG
Sbjct: 508 LKNWEEGGYFNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADFSEDENGQRWLCTGDIGE 567
Query: 576 FHPDGCLEIIDRKKDIVKLQHGEYISLGKVEAALASGDYVDSIMVHADPFHSYCVALVVA 635
F PDGCL+IIDRKKD+VKLQ GEY+SLGKVEAAL + VD+I +A+ +HSY + VV
Sbjct: 568 FEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSYHSYVIGFVVP 627
Query: 636 SHQSLEKWAQETGIEYKDFPDLCNKPEAVTEVLQSISKAAKAAKLQKTEVPAKIKLLADP 695
+ + L + A++ G++ + +LCN E EVL+ +S+AA +A L+K E+P KI+L +P
Sbjct: 628 NQKELTELARKKGLK-GTWEELCNSCEMENEVLKVLSEAAISASLEKFEIPVKIRLSPEP 686
Query: 696 WTPESGLVTAALKLK 710
WTPE+GLVT A KLK
Sbjct: 687 WTPETGLVTDAFKLK 701
>LCF4_RAT (O35547) Long-chain-fatty-acid--CoA ligase 4 (EC 6.2.1.3)
(Long-chain acyl-CoA synthetase 4) (LACS 4)
Length = 670
Score = 598 bits (1541), Expect = e-170
Identities = 309/636 (48%), Positives = 423/636 (65%), Gaps = 17/636 (2%)
Query: 79 LVEVPWKGAPTMAHLFEPSCKKHTHNRFLGTRKLIGKEFVTSSDGRKFEKVHLGEYEWET 138
++++P GA T+ LF+ + K LGTR+++ +E +G+ F+K+ LG Y+W
Sbjct: 29 VIDIP--GADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWIN 86
Query: 139 YGEVFARVSNFASGLLKLGHDIDSHVAIFSDTRAEWFIALQGCFRQNITVVTIYASLGED 198
Y EV RV+NF SGL LG + +AIF +TRAEW IA Q CF+ N +VT+YA+LG++
Sbjct: 87 YLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKE 146
Query: 199 ALIHSLNETQVSTLICDVKLL-NKLDAIRSKLTSLQNIIYFEDDSKEEHTFSEGLSSNCK 257
A++H LNE++ S LI V+LL +KL A + +++IIY ++ + + EGL +
Sbjct: 147 AVVHGLNESEASYLITSVELLESKLKAALLDINCVKHIIYVDNKTINRAEYPEGL----E 202
Query: 258 IASFDEVEKLGKE---SPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIVATTAAVM 314
I S VE+LG + S + PS P+ + +A+VMYTSGSTG PKGVM+ H N++A
Sbjct: 203 IHSMQSVEELGSKPENSSIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQC 262
Query: 315 TVIPNLGSKDVYLAYLPLAHVFEMAAESVMLAAGVAIGYGSPMTLTDTSNKVKKGTKGDV 374
IP LG KD Y+ YLPLAHV E+ AE G IGY SP+TL+D S+K+KKG+KGD
Sbjct: 263 ERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDC 322
Query: 375 TVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAVKGSWLGAWGVEK 434
TVLKPTL+ AVP I+DRI V+ KV+E + K LF+I Y +L +K G +
Sbjct: 323 TVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKK------GYDA 376
Query: 435 LVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIGQGYGLTETFAGAAF 494
+ + I+FKK++ +LGGN+R ML GGAPLS + +F+N+C PIGQGYGLTE+
Sbjct: 377 PLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTV 436
Query: 495 SEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFSVTAGYFKNQDKT 554
+E DY+ GRVG PL CC IKL W+EGGY DKP PRGE+V+GG +++ GYFKN++KT
Sbjct: 437 TEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKT 496
Query: 555 DEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYISLGKVEAALASGDY 614
E + VDE G RWF TGDIG FHPDGCL+IIDRKKD+VKLQ GEY+SLGKVEAAL +
Sbjct: 497 AEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPL 556
Query: 615 VDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNKPEAVTEVLQSISKA 674
+D+I A SY ++ VV + + L AQ+ G+E + D+CN P E+L+ I +A
Sbjct: 557 IDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVE-GSWVDICNNPAMEAEILKEIREA 615
Query: 675 AKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
A A KL++ E+P K++L +PWTPE+GLVT A KLK
Sbjct: 616 ANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLK 651
>LCF4_HUMAN (O60488) Long-chain-fatty-acid--CoA ligase 4 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 4) (LACS 4)
Length = 711
Score = 594 bits (1532), Expect = e-169
Identities = 314/697 (45%), Positives = 446/697 (63%), Gaps = 29/697 (4%)
Query: 30 TVFAIVIGIIVPVLFSVFFL-------GKKKG----KVRGVPVEVSGESGY-AVRNARYS 77
T+ + + +++ + ++ F+ KKK +++ P S Y +V +
Sbjct: 9 TIILLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSPYRSVTHFDSL 68
Query: 78 ELVEVPWKGAPTMAHLFEPSCKKHTHNRFLGTRKLIGKEFVTSSDGRKFEKVHLGEYEWE 137
++++P GA T+ LF+ + K LGTR+++ +E +G+ F+K+ LG Y+W
Sbjct: 69 AVIDIP--GADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWM 126
Query: 138 TYGEVFARVSNFASGLLKLGHDIDSHVAIFSDTRAEWFIALQGCFRQNITVVTIYASLGE 197
Y EV RV+NF SGL LG + +AIF +TRAEW IA Q CF+ N +VT+YA+LG+
Sbjct: 127 NYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGK 186
Query: 198 DALIHSLNETQVSTLICDVKLL-NKLDAIRSKLTSLQNIIYFEDDSKEEHTFSEGLSSNC 256
+A++H LNE++ S LI V+LL +KL ++ +++IIY ++ + + + EG
Sbjct: 187 EAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGF---- 242
Query: 257 KIASFDEVEKLGKESP---VEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIVATTAAV 313
+I S VE+LG + PS P+ + +A+VMYTSGSTG PKGVM+ H N++A
Sbjct: 243 EIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQ 302
Query: 314 MTVIPNLGSKDVYLAYLPLAHVFEMAAESVMLAAGVAIGYGSPMTLTDTSNKVKKGTKGD 373
IP LG KD Y+ YLPLAHV E+ AE G IGY SP+TL+D S+K+KKG+KGD
Sbjct: 303 CERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGD 362
Query: 374 VTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAVKGSWLGAWGVE 433
TVLKPTL+ AVP I+DRI V+ KV+E + K LF+I Y +L +K G +
Sbjct: 363 CTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKK------GYD 416
Query: 434 KLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIGQGYGLTETFAGAA 493
+ + ++FKK++ +LGGN+R ML GGAPLS + +F+N+C PIGQGYGLTE+
Sbjct: 417 APLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGT 476
Query: 494 FSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFSVTAGYFKNQDK 553
+E DY+ GRVG PL CC IKL W+EGGY +DKP PRGE+V+GG +++ GYFKN++K
Sbjct: 477 VTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEK 536
Query: 554 TDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYISLGKVEAALASGD 613
T E + VDE G RWF TGDIG FHPDGCL+IIDRKKD+VKLQ GEY+SLGKVEAAL +
Sbjct: 537 TAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCP 596
Query: 614 YVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNKPEAVTEVLQSISK 673
+D+I A SY ++ VV + + L AQ+ G+E + D+CN P E+L+ I +
Sbjct: 597 LIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVE-GTWVDICNNPAMEAEILKEIRE 655
Query: 674 AAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
AA A KL++ E+P K++L +PWTPE+GLVT A KLK
Sbjct: 656 AANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLK 692
>LCF3_MOUSE (Q9CZW4) Long-chain-fatty-acid--CoA ligase 3 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 3) (LACS 3)
Length = 720
Score = 592 bits (1526), Expect = e-168
Identities = 313/675 (46%), Positives = 432/675 (63%), Gaps = 16/675 (2%)
Query: 40 VPVLFSVFFLGKKKGKVRGVPVEVSGESGYAVRNARYSELVEVPWKGAPTMAHLFEPSCK 99
+P F +K +++ PV +S Y N+ L V + G T+ +F +
Sbjct: 39 IPFYFLCESKQEKPNQIKAKPVSSKPDSAYRSINS-VDGLASVLYPGCDTLDKVFMYAKN 97
Query: 100 KHTHNRFLGTRKLIGKEFVTSSDGRKFEKVHLGEYEWETYGEVFARVSNFASGLLKLGHD 159
K + R LGTR+++ +E +G+ F+KV LG Y W +Y +VF R +F +GL LG
Sbjct: 98 KFKNKRLLGTREILNEEDEIQPNGKIFKKVILGHYNWLSYEDVFIRALDFGNGLQMLGQK 157
Query: 160 IDSHVAIFSDTRAEWFIALQGCFRQNITVVTIYASLGEDALIHSLNETQVSTLICDVKLL 219
+++AIF +TRAEW IA Q CF N +VT+YA+LG A++H LNET+V+ +I +LL
Sbjct: 158 PKANIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHGLNETEVTNIITSKELL 217
Query: 220 N-KLDAIRSKLTSLQNIIYFEDDSKEEHTFSEGLSSNCKIASFDEVEKLGKESPVEPSLP 278
KL I S + L++II + F +G+ + A V+ LG ++ VE
Sbjct: 218 QTKLKEIVSLVPRLRHIITVDGKPPTWSEFPKGVIVHTMAA----VQALGVKANVEKKAH 273
Query: 279 SK---NAVAVVMYTSGSTGLPKGVMITHGNIVATTAAVMTVIPNLGSKDVYLAYLPLAHV 335
SK + +AV+MYTSGSTG+PKGVMI+H NI+A+ + IP LG +DVY+ YLPLAHV
Sbjct: 274 SKPLPSDIAVIMYTSGSTGIPKGVMISHSNIIASITGMARRIPRLGEEDVYIGYLPLAHV 333
Query: 336 FEMAAESVMLAAGVAIGYGSPMTLTDTSNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDG 395
E++AE V L+ G IGY SP TL D S+K+KKG+KGD +VLKPTL+ AVP I+DRI
Sbjct: 334 LELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSVLKPTLMAAVPEIMDRIYKN 393
Query: 396 VVKKVEEKGGLAKNLFQIAYKRRLAAVKGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRF 455
V+ KV E +NLF +AY ++ + G + D +F+ +R +LGGN+R
Sbjct: 394 VMNKVNEMSAFQRNLFILAYNYKMEQISK------GCSTPLCDRFVFRNVRRLLGGNIRL 447
Query: 456 MLCGGAPLSGDSQQFINICVGAPIGQGYGLTETFAGAAFSEADDYSVGRVGPPLPCCYIK 515
+LCGGAPLS +Q+F+NIC P+GQGYGLTE+ +E DY+ GRVG PL CC IK
Sbjct: 448 LLCGGAPLSATTQRFMNICFCCPVGQGYGLTESTGAGTITEVWDYNTGRVGAPLVCCEIK 507
Query: 516 LVSWEEGGYLSSDKPMPRGEVVVGGFSVTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGR 575
L +WEEGGY ++DKP PRGE+++GG +VT GY+KN+ KT F DE G RW TGDIG
Sbjct: 508 LKNWEEGGYFNTDKPHPRGEILIGGQNVTMGYYKNEAKTKTDFFEDENGQRWLCTGDIGE 567
Query: 576 FHPDGCLEIIDRKKDIVKLQHGEYISLGKVEAALASGDYVDSIMVHADPFHSYCVALVVA 635
F PDGCL+IIDRKKD+VKLQ GEY+SLGKVEAAL + +D+I +A+ +HSY + VV
Sbjct: 568 FDPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLIDNICAYANSYHSYVIGFVVP 627
Query: 636 SHQSLEKWAQETGIEYKDFPDLCNKPEAVTEVLQSISKAAKAAKLQKTEVPAKIKLLADP 695
+ + L + A+ G + + +LCN E EVL+ +S+AA +A L+K E+P KI+L DP
Sbjct: 628 NQKELTELARTKGFK-GTWEELCNSSEMENEVLKVLSEAAISASLEKFEIPLKIRLSPDP 686
Query: 696 WTPESGLVTAALKLK 710
WTPE+GLVT A KLK
Sbjct: 687 WTPETGLVTDAFKLK 701
>LCF4_MOUSE (Q9QUJ7) Long-chain-fatty-acid--CoA ligase 4 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 4) (LACS 4)
(mACS4)
Length = 711
Score = 590 bits (1521), Expect = e-168
Identities = 315/697 (45%), Positives = 445/697 (63%), Gaps = 29/697 (4%)
Query: 30 TVFAIVIGIIVPVLFSVFFL-------GKKKG----KVRGVPVEVSGESGY-AVRNARYS 77
T+ + + +++ + ++ F+ KKK +++ P S Y +V +
Sbjct: 9 TIILLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSPYRSVTHFDSL 68
Query: 78 ELVEVPWKGAPTMAHLFEPSCKKHTHNRFLGTRKLIGKEFVTSSDGRKFEKVHLGEYEWE 137
++++P GA T+ LF+ + K LGTR+++ +E +G+ F+K+ LG Y+W
Sbjct: 69 AVIDIP--GADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWI 126
Query: 138 TYGEVFARVSNFASGLLKLGHDIDSHVAIFSDTRAEWFIALQGCFRQNITVVTIYASLGE 197
Y EV RV+NF SGL LG + +AIF +TRAEW IA Q CF+ N +VT+YA+LG
Sbjct: 127 NYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGR 186
Query: 198 DALIHSLNETQVSTLICDVKLL-NKLDAIRSKLTSLQNIIYFEDDSKEEHTFSEGLSSNC 256
+A++H LNE++ S LI V+LL +KL A + +++IIY ++ + + EGL
Sbjct: 187 EAVVHGLNESEASYLITSVELLESKLKAALVDINCVKHIIYVDNKTINRAEYPEGL---- 242
Query: 257 KIASFDEVEKLGKESP---VEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIVATTAAV 313
+I S VE+LG + V PS P+ + +A+VMYTSGSTG PKG+M+ H N++A
Sbjct: 243 EIHSMQSVEELGAKPENLSVPPSRPTPSDMAIVMYTSGSTGRPKGLMMHHTNLIAGMTGQ 302
Query: 314 MTVIPNLGSKDVYLAYLPLAHVFEMAAESVMLAAGVAIGYGSPMTLTDTSNKVKKGTKGD 373
IP LG KD Y+ YLPLAHV E+ AE G IGY SP+TL+D S+K+KKG+KGD
Sbjct: 303 CERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGD 362
Query: 374 VTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAVKGSWLGAWGVE 433
TVLKPTL+ AVP I+DRI V+ KV+E + K LF+I Y +L +K G +
Sbjct: 363 CTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYVQKTLFKIGYDYKLEQIKK------GYD 416
Query: 434 KLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIGQGYGLTETFAGAA 493
+ + I+FKK++ ++GGN+R ML GGAPLS + +F+N+C+ PIGQGYGLTE+
Sbjct: 417 APLCNLILFKKVKDLVGGNVRMMLYGGAPLSPQTHRFMNVCLCCPIGQGYGLTESCGAGT 476
Query: 494 FSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFSVTAGYFKNQDK 553
+E DY+ GRVG PL CC IKL W+EGGY DKP PRGE+V+GG +++ GYFKN++K
Sbjct: 477 VTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEK 536
Query: 554 TDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYISLGKVEAALASGD 613
T E + VDE G RWF TGDIG FHPDGCL+IIDRKKD+VKLQ GEY+ LGKVEAAL +
Sbjct: 537 TAEDYCVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVFLGKVEAALKNCP 596
Query: 614 YVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNKPEAVTEVLQSISK 673
+D+I A SY ++ VV + + L AQ+ G+E + D+CN P E+L+ I +
Sbjct: 597 LIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVE-GSWVDICNNPAMEAEILKEIRE 655
Query: 674 AAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
AA A KL++ E+P K++L +PWTPE+GLVT A KLK
Sbjct: 656 AANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLK 692
>LCF3_RAT (Q63151) Long-chain-fatty-acid--CoA ligase 3 (EC 6.2.1.3)
(Long-chain acyl-CoA synthetase 3) (LACS 3) (Brain
acyl-CoA synthtase II)
Length = 720
Score = 589 bits (1518), Expect = e-167
Identities = 312/675 (46%), Positives = 429/675 (63%), Gaps = 16/675 (2%)
Query: 40 VPVLFSVFFLGKKKGKVRGVPVEVSGESGYAVRNARYSELVEVPWKGAPTMAHLFEPSCK 99
+P F +K ++ PV +S Y N+ L V + G T+ +F +
Sbjct: 39 IPFYFLCESKQEKPNHIKAKPVSSKPDSAYRSVNSM-DGLASVLYPGCDTLDKVFMYAKN 97
Query: 100 KHTHNRFLGTRKLIGKEFVTSSDGRKFEKVHLGEYEWETYGEVFARVSNFASGLLKLGHD 159
K R LGTR+++ +E +G+ F+KV LG Y W +Y +VF R +F +GL LG
Sbjct: 98 KFKDKRLLGTREILNEEDEIQPNGKVFKKVILGHYNWLSYEDVFIRALDFGNGLQMLGQK 157
Query: 160 IDSHVAIFSDTRAEWFIALQGCFRQNITVVTIYASLGEDALIHSLNETQVSTLICDVKLL 219
+++AIF +TRAEW IA Q CF N +VT+YA+LG A++H LNET+V+ +I +LL
Sbjct: 158 PKANIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHGLNETEVTNIITSKELL 217
Query: 220 N-KLDAIRSKLTSLQNIIYFEDDSKEEHTFSEGLSSNCKIASFDEVEKLGKESPVEPSLP 278
KL I S + L++II + F +G+ + A V+ LG ++ V+
Sbjct: 218 QTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGVIVHTMAA----VQALGVKADVDKKAH 273
Query: 279 SK---NAVAVVMYTSGSTGLPKGVMITHGNIVATTAAVMTVIPNLGSKDVYLAYLPLAHV 335
SK + +AV+MYTSGSTG+PKGVMI+H NI+A+ + IP LG +DVY+ YLPLAHV
Sbjct: 274 SKPLPSDIAVIMYTSGSTGIPKGVMISHSNIIASITGMARRIPRLGEEDVYIGYLPLAHV 333
Query: 336 FEMAAESVMLAAGVAIGYGSPMTLTDTSNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDG 395
E++AE V L+ G IGY SP TL D S+K+KKG+KGD +VLKPTL+ AVP I+DRI
Sbjct: 334 LELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSVLKPTLMAAVPEIMDRIYKN 393
Query: 396 VVKKVEEKGGLAKNLFQIAYKRRLAAVKGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRF 455
V+ KV E +NLF +AY ++ + G + D +F+ +R +LGGN+R
Sbjct: 394 VMNKVNEMSAFQRNLFILAYNYKMEQISK------GCSTPLCDRFVFRNVRRLLGGNIRV 447
Query: 456 MLCGGAPLSGDSQQFINICVGAPIGQGYGLTETFAGAAFSEADDYSVGRVGPPLPCCYIK 515
+LCGGAPLS +Q+F+NIC P+GQGYGLTE+ +E DY+ GRVG PL CC IK
Sbjct: 448 LLCGGAPLSATTQRFMNICFCCPVGQGYGLTESTGAGTITEVWDYNTGRVGAPLVCCEIK 507
Query: 516 LVSWEEGGYLSSDKPMPRGEVVVGGFSVTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGR 575
L +WEEGGY ++DKP PRGE+++GG +VT GY+KN+ KT F DE G RW TGDIG
Sbjct: 508 LKNWEEGGYFNTDKPHPRGEILIGGQNVTMGYYKNEAKTKADFFEDENGQRWLCTGDIGE 567
Query: 576 FHPDGCLEIIDRKKDIVKLQHGEYISLGKVEAALASGDYVDSIMVHADPFHSYCVALVVA 635
F PDGCL+IIDRKKD+VKLQ GEY+SLGKVEAAL + +D+I +A+ +HSY + VV
Sbjct: 568 FDPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLIDNICAYANSYHSYVIGFVVP 627
Query: 636 SHQSLEKWAQETGIEYKDFPDLCNKPEAVTEVLQSISKAAKAAKLQKTEVPAKIKLLADP 695
+ + L + A+ G + +LCN E EVL+ +S+AA +A L+K E+P KI+L DP
Sbjct: 628 NQKELTELARTKGFN-GTWEELCNSSEMENEVLKVLSEAAISASLEKFEIPLKIRLSPDP 686
Query: 696 WTPESGLVTAALKLK 710
WTPE+GLVT A KLK
Sbjct: 687 WTPETGLVTDAFKLK 701
>LCF1_YEAST (P30624) Long-chain-fatty-acid--CoA ligase 1 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 1) (Fatty acid
activator 1)
Length = 700
Score = 326 bits (836), Expect = 1e-88
Identities = 215/625 (34%), Positives = 332/625 (52%), Gaps = 43/625 (6%)
Query: 110 RKLIGKEFVTSSDGRKFEKVHLGEYEWETYGEVFARVSNFASGLLKLG--HDIDSHVAIF 167
+K+ GKE +S +K+ L Y + ++ ++ + GL+K+G + D + ++
Sbjct: 76 KKVDGKE---TSVEKKWMYYELSHYHYNSFDQLTDIMHEIGRGLVKIGLKPNDDDKLHLY 132
Query: 168 SDTRAEWFIALQGCFRQNITVVTIYASLGEDALIHSLNETQVSTLICDVKLLNKLDAIRS 227
+ T +W G Q I VVT Y +LGE LIHSL +T + D LL L
Sbjct: 133 AATSHKWMKMFLGAQSQGIPVVTAYDTLGEKGLIHSLVQTGSKAIFTDNSLLPSLIKPVQ 192
Query: 228 KLTSLQNIIYFEDDSKEEHTFS--------------EGLSSNCKIASFDEVEKLGKES-- 271
++ II+F+ S E+ S + + + K SFD++ KLGKES
Sbjct: 193 AAQDVKYIIHFDSISSEDRRQSGKIYQSAHDAINRIKEVRPDIKTFSFDDILKLGKESCN 252
Query: 272 PVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIVATTA-AVMTVIPNLGSKDVYLAYL 330
++ P K+ + +MYTSGSTG PKGV++ H N+VA A + V+ +G+ D + +L
Sbjct: 253 EIDVHPPGKDDLCCIMYTSGSTGEPKGVVLKHSNVVAGVGGASLNVLKFVGNTDRVICFL 312
Query: 331 PLAHVFEMAAESVMLAAGVAIGYGSPMTLTDTSNKVKKGTKGDVTVLKPTLLTAVPAIID 390
PLAH+FE+ E + G IGY + TLT +S + +GD+ KPT++ V A+ +
Sbjct: 313 PLAHIFELVFELLSFYWGACIGYATVKTLTSSS---VRNCQGDLQEFKPTIMVGVAAVWE 369
Query: 391 RIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAVKGSWLGAWGVEKLVWDTIIFKKIRTVLG 450
+R G++ +++ L K +F AY +L + G + LV FKKIRT G
Sbjct: 370 TVRKGILNQIDNLPFLTKKIFWTAYNTKLNMQRLHIPGGGALGNLV-----FKKIRTATG 424
Query: 451 GNLRFMLCGGAPLSGDSQQFINICVGAPIGQGYGLTETFAGAAFSEADDYSVGRVGPPLP 510
G LR++L GG+P+S D+Q+FI + P+ GYGLTET A + ++ +G G
Sbjct: 425 GQLRYLLNGGSPISRDAQEFITNLI-CPMLIGYGLTETCASTTILDPANFELGVAGDLTG 483
Query: 511 CCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFSVTAGYFKNQDKTDEVFKVDEKGVRWFYT 570
C +KLV EE GY + + +GEV + G +VT Y+KN+++T + D WF T
Sbjct: 484 CVTVKLVDVEELGYFAKNN---QGEVWITGANVTPEYYKNEEETSQALTSDG----WFKT 536
Query: 571 GDIGRFHPDGCLEIIDRKKDIVKLQHGEYISLGKVEAALASGDYVDSIMVHADPFHSYCV 630
GDIG + +G L+IIDRKK++VK +GEYI+L K+E+ S +YV +I V+AD + V
Sbjct: 537 GDIGEWEANGHLKIIDRKKNLVKTMNGEYIALEKLESVYRSNEYVANICVYADQSKTKPV 596
Query: 631 ALVVASHQSLEKWAQETGI-EYKD----FPDLCNKPEAVTEVLQSISKAAKAAKLQKTEV 685
++V +H L K A++ GI E KD + + + V + K K L E+
Sbjct: 597 GIIVPNHAPLTKLAKKLGIMEQKDSSINIENYLEDAKLIKAVYSDLLKTGKDQGLVGIEL 656
Query: 686 PAKIKLLADPWTPESGLVTAALKLK 710
A I WTP++G VT+A KLK
Sbjct: 657 LAGIVFFDGEWTPQNGFVTSAQKLK 681
>LCF2_MOUSE (P41216) Long-chain-fatty-acid--CoA ligase 2 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 2) (LACS 2)
Length = 699
Score = 324 bits (830), Expect = 6e-88
Identities = 206/588 (35%), Positives = 309/588 (52%), Gaps = 39/588 (6%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGHD--IDSHVAIFSDTRAEWFIALQGCFRQNITVVTI 191
YEW +Y EV SGL++ G + + +FS R EW I QGCF ++ VV +
Sbjct: 119 YEWISYKEVAELAECIGSGLIQKGFKPCSEQFIGLFSQNRPEWVIVEQGCFSYSMVVVPL 178
Query: 192 YASLGEDALIHSLNETQVSTLICD----VKLLNKLDAIRSKLTSLQNIIYFEDDSKEEHT 247
Y +LG DA+ + +N+ ++S + D KLL L+ + +KLT II D S
Sbjct: 179 YDTLGADAITYIVNKAELSVIFADKPEKAKLL--LEGVENKLTPCLKIIVIMD-SYGSDL 235
Query: 248 FSEGLSSNCKIASFDEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIV 307
G +I S +E LG+ + V+P P +A++ +TSG+TG PKG MITH NI+
Sbjct: 236 VERGKKCGVEIISLKALEDLGRVNRVKPKPPEPEDLAIICFTSGTTGNPKGAMITHQNII 295
Query: 308 ATTAAVMTVIPNL---GSKDVYLAYLPLAHVFEMAAESVMLAAGVAIGY--GSPMTLTDT 362
+ + + + DV +++LPLAH+FE E VML G IG+ G L D
Sbjct: 296 NDCSGFIKATESAFIASTDDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMD- 354
Query: 363 SNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAV 422
D+ VL+PT+ VP +++R+ D + + L + L A KR+ A V
Sbjct: 355 ----------DLKVLQPTIFPVVPRLLNRMFDRIFGQANTS--LKRWLLDFASKRKEADV 402
Query: 423 KGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIGQG 482
+ + +WD +IF KI++ LGG +R M+ G AP+S F+ +G +G
Sbjct: 403 RSGIVR----NNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRTALGCQFYEG 458
Query: 483 YGLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFS 542
YG TE AG S D++ G VG P+PC Y+KLV EE YL+S GEV V G +
Sbjct: 459 YGQTECTAGCCLSLPGDWTAGHVGAPMPCNYVKLVDVEEMNYLAS---KGEGEVCVKGAN 515
Query: 543 VTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYISL 602
V GY K+ +T E +D+ G W +TGDIG++ P+G L+IIDRKK I KL GEYI+
Sbjct: 516 VFKGYLKDPARTAEA--LDKDG--WLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAP 571
Query: 603 GKVEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNKPE 662
K+E + V + VH + ++ +A+VV +SL WAQ+ G++ F +LC +
Sbjct: 572 EKIENIYLRSEAVAQVFVHGESLQAFLIAVVVPDVESLPSWAQKRGLQ-GSFEELCRNKD 630
Query: 663 AVTEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
+L + K K A L+ E I + + ++ ++GL+T LK K
Sbjct: 631 INKAILDDLLKLGKEAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAK 678
>LCF1_CAVPO (Q9JID6) Long-chain-fatty-acid--CoA ligase 1 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 1) (LACS 1)
(Palmitoyl-CoA ligase)
Length = 698
Score = 316 bits (809), Expect = 2e-85
Identities = 197/586 (33%), Positives = 311/586 (52%), Gaps = 35/586 (5%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGHDI--DSHVAIFSDTRAEWFIALQGCFRQNITVVTI 191
YEW +Y +V S LL+ G D + IF+ R EW I Q CF ++ VV +
Sbjct: 118 YEWLSYKQVEDLSECIGSALLQKGFQASPDQFIGIFAQNRPEWVIIEQACFAYSMVVVPL 177
Query: 192 YASLGEDALIHSLNETQVSTLICDV--KLLNKLDAIRSKLTSLQNIIYFEDDSKEEHTFS 249
Y +LG DA+ + +N+ ++S + D K L+++ +KLT II D E
Sbjct: 178 YDTLGADAITYIVNKAELSVIFADKPEKARILLESVENKLTPGLKIIVVMDSYGSE-LVE 236
Query: 250 EGLSSNCKIASFDEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIVAT 309
+G ++ S +E LG+ + +P P + +AV+ +TSG+TG PKG MITH N+V+
Sbjct: 237 QGKKCGVEVISLKAMEGLGRANRRKPKPPEPDDLAVICFTSGTTGNPKGAMITHKNVVSD 296
Query: 310 TAAVMTVIPN---LGSKDVYLAYLPLAHVFEMAAESVMLAAGVAIGY--GSPMTLTDTSN 364
+A + L + D+++++LPLAH++E + VML G IG+ G L D
Sbjct: 297 CSAFVKATEKALVLNASDIHISFLPLAHMYEQLLQCVMLCHGAKIGFFQGDIRLLMD--- 353
Query: 365 KVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAVKG 424
D+ L+PT+ VP +++R+ D + + + + L A KR+ A ++
Sbjct: 354 --------DLKALQPTIFPVVPRLLNRMFDRIFAQANTT--VKRWLLDFASKRKEAELRS 403
Query: 425 SWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIGQGYG 484
+ VWD +IF KI++ LGG +R M+ G AP+S F+ +G +GYG
Sbjct: 404 GIIR----NNSVWDKLIFHKIQSSLGGKVRLMVTGAAPVSATVLTFLRAALGCQFYEGYG 459
Query: 485 LTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFSVT 544
TE AG + S D++ G VG P+PC +IKLV EE Y+++ M GEV V G +V
Sbjct: 460 QTECTAGCSLSVPGDWTAGHVGAPMPCNFIKLVDVEEMNYMAA---MGEGEVCVKGPNVF 516
Query: 545 AGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYISLGK 604
GY K+ KT E +D+ G W +TGDIG++ P+G L+IIDRKK I KL GEYI+ K
Sbjct: 517 KGYLKDPAKTAEA--LDKDG--WLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEK 572
Query: 605 VEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNKPEAV 664
+E + V + VH + ++ +A+VV +SL WA++ G E F +LC +
Sbjct: 573 IENIYVRSEPVAQVFVHGESLQAFLIAIVVPDAESLASWARKRGFE-GSFEELCRNKDVK 631
Query: 665 TEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
+L+ + + K + L+ E I L + ++ ++GL+T +K K
Sbjct: 632 KAILEDMVRIGKDSGLKSFEQVRGIALHPELFSVDNGLLTPTMKAK 677
>LCF2_RAT (P18163) Long-chain-fatty-acid--CoA ligase, liver isozyme
(EC 6.2.1.3) (Long-chain acyl-CoA synthetase 2) (LACS 2)
Length = 699
Score = 308 bits (788), Expect = 4e-83
Identities = 196/588 (33%), Positives = 304/588 (51%), Gaps = 39/588 (6%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGHD--IDSHVAIFSDTRAEWFIALQGCFRQNITVVTI 191
YEW +Y +V S L++ G + + IFS R EW QGCF ++ VV +
Sbjct: 119 YEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVVVPL 178
Query: 192 YASLGEDALIHSLNETQVSTLICD----VKLLNKLDAIRSKLTSLQNIIYFEDDSKEEHT 247
Y +LG DA+ + +N+ ++S + D KLL L+ + +KLT II D S +
Sbjct: 179 YDTLGTDAITYIVNKAELSVIFADKPEKAKLL--LEGVENKLTPCLKIIVIMD-SYDNDL 235
Query: 248 FSEGLSSNCKIASFDEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIV 307
G +I +E LG+ + +P P +A++ +TSG+TG PKG M+TH NI+
Sbjct: 236 VERGQKCGVEIIGLKALEDLGRVNRTKPKPPEPEDLAIICFTSGTTGNPKGAMVTHQNIM 295
Query: 308 ATTAAVMTVIPNL---GSKDVYLAYLPLAHVFEMAAESVMLAAGVAIGY--GSPMTLTDT 362
+ + + +DV +++LPLAH+FE E VML G IG+ G L D
Sbjct: 296 NDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIRLLMD- 354
Query: 363 SNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAV 422
D+ VL+PT+ VP +++R+ D + + + + L A KR+ A +
Sbjct: 355 ----------DLKVLQPTIFPVVPRLLNRMFDRIFGQANTS--VKRWLLDFASKRKEAEL 402
Query: 423 KGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIGQG 482
+ + +WD +IF KI++ LGG +R M+ G AP+S F+ +G +G
Sbjct: 403 RSGIVR----NNSLWDKLIFHKIQSSLGGKVRLMITGAAPVSATVLTFLRAALGCQFYEG 458
Query: 483 YGLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFS 542
YG TE AG S D++ G VG P+PC YIKLV E+ Y ++ GEV V G +
Sbjct: 459 YGQTECTAGCCLSLPGDWTAGHVGAPMPCNYIKLVDVEDMNYQAA---KGEGEVCVKGAN 515
Query: 543 VTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYISL 602
V GY K+ +T E +D+ G W +TGDIG++ P+G L+IIDRKK I KL GEYI+
Sbjct: 516 VFKGYLKDPARTAEA--LDKDG--WLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAP 571
Query: 603 GKVEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNKPE 662
K+E + V + VH + ++ +A+VV + L WAQ+ G + F +LC +
Sbjct: 572 EKIENIYLRSEAVAQVFVHGESLQAFLIAIVVPDVEILPSWAQKRGFQ-GSFEELCRNKD 630
Query: 663 AVTEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
+L+ + K K A L+ E I + + ++ ++GL+T LK K
Sbjct: 631 INKAILEDMVKLGKNAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAK 678
>LCF6_RAT (P33124) Long-chain-fatty-acid--CoA ligase 6 (EC 6.2.1.3)
(Long-chain acyl-CoA synthetase 6) (LACS 6)
(Long-chain-fatty-acid--CoA ligase, brain isozyme)
Length = 697
Score = 307 bits (787), Expect = 6e-83
Identities = 198/591 (33%), Positives = 308/591 (51%), Gaps = 45/591 (7%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGHDI----DSHVAIFSDTRAEWFIALQGCFRQNITVV 189
Y+W +Y EV R SGLL+ HD + + +F+ R EW IA C+ ++ VV
Sbjct: 118 YQWLSYQEVAKRAEFLGSGLLQ--HDCKVGTEQFIGVFAQNRPEWIIAELACYTYSMVVV 175
Query: 190 TIYASLGEDALIHSLNETQVSTLICDV--KLLNKLDAIRSKLT-SLQNIIYFE--DDSKE 244
+Y +LG ++ + +N + T+I D K + L+ + K T L+ +I E DD+
Sbjct: 176 PLYDTLGPGSIRYIINTADICTVIVDKPHKAILLLEHVERKETPGLKLVILMEPFDDALR 235
Query: 245 EHTFSEGLSSNCKIASFDEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHG 304
E G I S +E G+E+ P P + +++V +TSG+TG PKG M+THG
Sbjct: 236 ER----GKKCGVDIKSMQAIEDSGQENHRVPVPPRPDDLSIVCFTSGTTGNPKGAMLTHG 291
Query: 305 NIVATTAAVMTVIPNLGSK---DVYLAYLPLAHVFEMAAESVMLAAGVAIGY--GSPMTL 359
N+VA + + V + + DV+ +YLPLAH+FE +SV+ G +G+ G L
Sbjct: 292 NVVADFSGFLKVTESQWAPTCADVHFSYLPLAHMFERMVQSVVYCHGGRVGFFQGDIRLL 351
Query: 360 TDTSNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRL 419
+D D+ L+PT+ VP +++R+ D + + + L + L + A KR+
Sbjct: 352 SD-----------DMKALRPTIFPVVPRLLNRMYDKIFHQADTS--LKRWLLEFAAKRKQ 398
Query: 420 AAVKGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPI 479
A V+ + +WD + F KI+ LGG++R ++ G AP S F+ +G +
Sbjct: 399 AEVRSGIIR----NNSIWDELFFNKIQASLGGHVRMIVTGAAPASPTVLGFLRAALGCQV 454
Query: 480 GQGYGLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVG 539
+GYG TE AG F+ D++ G VG PLPC +IKLV EE Y +S GE+ V
Sbjct: 455 YEGYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIKLVDAEELNYWTS---KGEGEICVK 511
Query: 540 GFSVTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEY 599
G +V GY K++D+T E D W +TGDIG++ P+G L+IIDRKK I KL GEY
Sbjct: 512 GPNVFKGYLKDEDRTKEALDSD----GWLHTGDIGKWLPEGTLKIIDRKKHIFKLAQGEY 567
Query: 600 ISLGKVEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCN 659
++ K+E + V I VH D ++ V +VV + + WAQ+ GIE ++ +LC
Sbjct: 568 VAPEKIENIYIRSEPVAQIYVHGDSLKAFLVGIVVPDPEVMPCWAQKKGIE-GNYQELCK 626
Query: 660 KPEAVTEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
E +L + K + L E I + D ++ ++GL+T LK K
Sbjct: 627 SKELKKAILDDMVMLGKESGLHSFEQVKAIYIHCDMFSVQNGLLTPTLKAK 677
>LCF1_HUMAN (P33121) Long-chain-fatty-acid--CoA ligase 1 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 1) (LACS 1)
(Palmitoyl-CoA ligase 1) (Long-chain fatty acid CoA
ligase 2) (Long-chain acyl-CoA synthetase 2) (LACS 2)
(Acyl-CoA synthetase 1) (ACS1) (Pal
Length = 698
Score = 305 bits (782), Expect = 2e-82
Identities = 196/588 (33%), Positives = 308/588 (52%), Gaps = 39/588 (6%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGHDI--DSHVAIFSDTRAEWFIALQGCFRQNITVVTI 191
YEW +Y +V S L++ G D + IF+ R EW I QGCF ++ +V +
Sbjct: 118 YEWLSYKQVAELSECIGSALIQKGFKTAPDQFIGIFAQNRPEWVIIEQGCFAYSMVIVPL 177
Query: 192 YASLGEDALIHSLNETQVSTLICD----VKLLNKLDAIRSKLTSLQNIIYFEDDSKEEHT 247
Y +LG +A+ + +N+ ++S + D KLL L+ + +KL II D E
Sbjct: 178 YDTLGNEAITYIVNKAELSLVFVDKPEKAKLL--LEGVENKLIPGLKIIVVMDAYGSE-L 234
Query: 248 FSEGLSSNCKIASFDEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIV 307
G ++ S +E LG+ + +P P+ +AV+ +TSG+TG PKG M+TH NIV
Sbjct: 235 VERGQRCGVEVTSMKAMEDLGRANRRKPKPPAPEDLAVICFTSGTTGNPKGAMVTHRNIV 294
Query: 308 ATTAAVMTVIPNLGSK---DVYLAYLPLAHVFEMAAESVMLAAGVAIGY--GSPMTLTDT 362
+ +A + N + D +++LPLAH+FE E VML G IG+ G L D
Sbjct: 295 SDCSAFVKATENTVNPCPDDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMD- 353
Query: 363 SNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAV 422
D+ VL+PT+ VP +++R+ D + + L + L A KR+ A +
Sbjct: 354 ----------DLKVLQPTVFPVVPRLLNRMFDRIFGQANTT--LKRWLLDFASKRKEAEL 401
Query: 423 KGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIGQG 482
+ + +WD +IF K+++ LGG +R M+ G AP+S F+ +G +G
Sbjct: 402 RSGIIR----NNSLWDRLIFHKVQSSLGGRVRLMVTGAAPVSATVLTFLRAALGCQFYEG 457
Query: 483 YGLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFS 542
YG TE AG + D++ G VG P+PC IKLV EE Y++++ GEV V G +
Sbjct: 458 YGQTECTAGCCLTMPGDWTAGHVGAPMPCNLIKLVDVEEMNYMAAE---GEGEVCVKGPN 514
Query: 543 VTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYISL 602
V GY K+ KT E +D+ G W +TGDIG++ P+G L+IIDRKK I KL GEYI+
Sbjct: 515 VFQGYLKDPAKTAEA--LDKDG--WLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAP 570
Query: 603 GKVEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNKPE 662
K+E + V + VH + ++ +A+VV ++L WAQ+ G E F +LC +
Sbjct: 571 EKIENIYMRSEPVAQVFVHGESLQAFLIAIVVPDVETLCSWAQKRGFE-GSFEELCRNKD 629
Query: 663 AVTEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
+L+ + + K + L+ E I L + ++ ++GL+T +K K
Sbjct: 630 VKKAILEDMVRLGKDSGLKPFEQVKGITLHPELFSIDNGLLTPTMKAK 677
>LCF6_MOUSE (Q91WC3) Long-chain-fatty-acid--CoA ligase 6 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 6) (LACS 6)
Length = 697
Score = 305 bits (781), Expect = 3e-82
Identities = 196/592 (33%), Positives = 303/592 (51%), Gaps = 47/592 (7%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGHDI----DSHVAIFSDTRAEWFIALQGCFRQNITVV 189
Y+W +Y EV R SGLL+ HD + V +F+ R EW IA C+ ++ VV
Sbjct: 118 YQWLSYQEVAKRAEFLGSGLLQ--HDCKVGTEQFVGVFAQNRPEWIIAELACYTYSMVVV 175
Query: 190 TIYASLGEDALIHSLNETQVSTLICDV--KLLNKLDAIRSKLTSLQNIIY----FEDDSK 243
+Y +LG ++ + +N + T+I D K L+ + K T ++ FED +
Sbjct: 176 PLYDTLGPGSISYIINTADICTVIVDKPHKATLLLEHVERKETPGLKLVILMEPFEDALR 235
Query: 244 EEHTFSEGLSSNCKIASFDEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITH 303
E G I S +E G+E+ P P + +++V +TSG+TG PKG M+TH
Sbjct: 236 ER-----GKKCGVDIKSMQAIEDCGRENHHAPVPPRPDDLSIVCFTSGTTGNPKGAMLTH 290
Query: 304 GNIVATTAAVMTVIPNL---GSKDVYLAYLPLAHVFEMAAESVMLAAGVAIGY--GSPMT 358
GN+VA + + V + DV +++LPLAH+FE +SV+ G +G+ G
Sbjct: 291 GNVVADFSGFLKVTEKVIFPRQDDVLISFLPLAHMFERVIQSVVYCHGGRVGFFQGDIRL 350
Query: 359 LTDTSNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRR 418
L+D D+ L+PT+ VP +++R+ D + + + L + L + A KR+
Sbjct: 351 LSD-----------DMKALRPTIFPVVPRLLNRMYDKIFHQADTS--LKRWLLEFAAKRK 397
Query: 419 LAAVKGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAP 478
A V+ + +WD + F KI+ LGG++R ++ G AP S F+ +G
Sbjct: 398 QAEVRSGIIR----NNSIWDELFFNKIQASLGGHVRMIVTGAAPASPTVLGFLRAALGCQ 453
Query: 479 IGQGYGLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVV 538
+ +GYG TE AG F+ D++ G VG PLPC +IKLV EE Y + GE+ V
Sbjct: 454 VYEGYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIKLVDAEELNYWTC---KGEGEICV 510
Query: 539 GGFSVTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGE 598
G +V GY K++D+T E D W +TGDIG++ P+G L+IIDRKK I KL GE
Sbjct: 511 KGPNVFKGYLKDEDRTKEALDSD----GWLHTGDIGKWLPEGTLKIIDRKKHIFKLAQGE 566
Query: 599 YISLGKVEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLC 658
Y++ K+E + V I VH D ++ V +VV + + WAQ+ GIE + +LC
Sbjct: 567 YVAPEKIENIYIRSEPVAQIYVHGDSLKAFLVGIVVPDPEVMPSWAQKKGIE-GTYQELC 625
Query: 659 NKPEAVTEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
K E +L + K + L E I + D ++ ++GL+T LK K
Sbjct: 626 MKKELKKAILDDMVMLGKESGLHSFEQVKAIYIHCDMFSVQNGLLTPTLKAK 677
>LCF6_HUMAN (Q9UKU0) Long-chain-fatty-acid--CoA ligase 6 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 6) (LACS 6)
Length = 697
Score = 304 bits (779), Expect = 5e-82
Identities = 197/588 (33%), Positives = 300/588 (50%), Gaps = 39/588 (6%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGHDI----DSHVAIFSDTRAEWFIALQGCFRQNITVV 189
Y+W +Y EV R SGLL+ H+ D + +F+ R EW I C+ ++ VV
Sbjct: 118 YQWLSYQEVADRAEFLGSGLLQ--HNCKACTDQFIGVFAQNRPEWIIVELACYTYSMVVV 175
Query: 190 TIYASLGEDALIHSLNETQVSTLICDV--KLLNKLDAIRSKLTSLQNIIYFEDDSKEEHT 247
+Y +LG A+ + +N +ST+I D K + L+ + K T +I D EE
Sbjct: 176 PLYDTLGPGAIRYIINTADISTVIVDKPQKAVLLLEHVERKETPGLKLIILMDPF-EEAL 234
Query: 248 FSEGLSSNCKIASFDEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIV 307
G I S VE G+E+ P P + +++V +TSG+TG PKG M+THGN+V
Sbjct: 235 KERGQKCGVVIKSMQAVEDCGQENHQAPVPPQPDDLSIVCFTSGTTGNPKGAMLTHGNVV 294
Query: 308 ATTAAVMTVIPNLGSK---DVYLAYLPLAHVFEMAAESVMLAAGVAIGY--GSPMTLTDT 362
A + + V + + DV+++YLPLAH+FE +SV+ G +G+ G L+D
Sbjct: 295 ADFSGFLKVTESQWAPTCADVHISYLPLAHMFERMVQSVVYCHGGRVGFFQGDIRLLSD- 353
Query: 363 SNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAV 422
D+ L PT+ VP +++R+ D + + L + L + A KR+ A V
Sbjct: 354 ----------DMKALCPTIFPVVPRLLNRMYDKIFSQANTP--LKRWLLEFAAKRKQAEV 401
Query: 423 KGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIGQG 482
+ + +WD + F KI+ LGG +R ++ G AP S F+ +G + +G
Sbjct: 402 RSGIIR----NDSIWDELFFNKIQASLGGCVRMIVTGAAPASPTVLGFLRAALGCQVYEG 457
Query: 483 YGLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFS 542
YG TE AG F+ D++ G VG PLPC +IKLV EE Y + GE+ V G +
Sbjct: 458 YGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIKLVDVEELNYWAC---KGEGEICVRGPN 514
Query: 543 VTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYISL 602
V GY K+ D+T E D W +TGDIG++ P G L+IIDRKK I KL GEY++
Sbjct: 515 VFKGYLKDPDRTKEALDSD----GWLHTGDIGKWLPAGTLKIIDRKKHIFKLAQGEYVAP 570
Query: 603 GKVEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNKPE 662
K+E V I VH D ++ V +VV + + WAQ+ GIE + DLC +
Sbjct: 571 EKIENIYIRSQPVAQIYVHGDSLKAFLVGIVVPDPEVMPSWAQKRGIE-GTYADLCTNKD 629
Query: 663 AVTEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
+L+ + + K + L E I + +D ++ ++GL+T LK K
Sbjct: 630 LKKAILEDMVRLGKESGLHSFEQVKAIHIHSDMFSVQNGLLTPTLKAK 677
>LCF5_HUMAN (Q9ULC5) Long-chain-fatty-acid--CoA ligase 5 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 5) (LACS 5)
Length = 683
Score = 304 bits (779), Expect = 5e-82
Identities = 203/590 (34%), Positives = 301/590 (50%), Gaps = 43/590 (7%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGHDI--DSHVAIFSDTRAEWFIALQGCFRQNITVVTI 191
Y W +Y +V R S LL G+ D V IF+ R EW I+ C+ ++ V +
Sbjct: 103 YRWLSYKQVSDRAEYLGSCLLHKGYKSSPDQFVGIFAQNRPEWIISELACYTYSMVAVPL 162
Query: 192 YASLGEDALIHSLNETQVSTLICDV--KLLNKLDAIRSKLT-SLQNIIY---FEDDSKEE 245
Y +LG +A++H +N+ ++ +ICD K L + + T SL+ II F+DD K+
Sbjct: 163 YDTLGPEAIVHIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQR 222
Query: 246 HTFSEGLSSNCKIASFDEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGN 305
G S +I S + E LGKE +P PS ++V+ +TSG+TG PKG MITH N
Sbjct: 223 -----GEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPKGAMITHQN 277
Query: 306 IVATTAAVMTVIPNLGSK---DVYLAYLPLAHVFEMAAESVMLAAGVAIGY--GSPMTLT 360
IV+ AA + + + DV ++YLPLAH+FE ++V+ + G +G+ G L
Sbjct: 278 IVSNAAAFLKCVEHAYEPTPDDVAISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLA 337
Query: 361 DTSNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLA 420
D D+ LKPTL AVP +++RI D V E K L K L ++A +
Sbjct: 338 D-----------DMKTLKPTLFPAVPRLLNRIYDKVQN--EAKTPLKKFLLKLAVSSKFK 384
Query: 421 AVKGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIG 480
++ + WD +IF KI+ LGG +R ++ G AP+S F +G +
Sbjct: 385 ELQKGIIR----HDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVY 440
Query: 481 QGYGLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGG 540
+ YG TE G F+ D++ G VG PL C Y+KL + Y + + GEV + G
Sbjct: 441 EAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNN---EGEVCIKG 497
Query: 541 FSVTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYI 600
+V GY K+ +KT E D W +TGDIGR+ P+G L+IIDRKK+I KL GEYI
Sbjct: 498 TNVFKGYLKDPEKTQEALDSD----GWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYI 553
Query: 601 SLGKVEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNK 660
+ K+E V I VH + S V +VV L +A + G++ F +LC
Sbjct: 554 APEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVK-GSFEELCQN 612
Query: 661 PEAVTEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
+L+ + K K + L+ E I L +P++ E+GL+T LK K
Sbjct: 613 QVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAK 662
>LCF5_RAT (O88813) Long-chain-fatty-acid--CoA ligase 5 (EC 6.2.1.3)
(Long-chain acyl-CoA synthetase 5) (LACS 5)
Length = 683
Score = 303 bits (777), Expect = 8e-82
Identities = 190/587 (32%), Positives = 306/587 (51%), Gaps = 37/587 (6%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGH--DIDSHVAIFSDTRAEWFIALQGCFRQNITVVTI 191
Y+W +Y +V R S LL G+ D + IF+ R EW I+ C+ ++ V +
Sbjct: 103 YKWISYKQVSDRAEYLGSCLLHKGYKPSQDQFIGIFAQNRPEWVISELACYTYSMVAVPL 162
Query: 192 YASLGEDALIHSLNETQVSTLICDV--KLLNKLDAIRSKLT-SLQNIIYFEDDSKEEHTF 248
Y +LG +A+I+ +N +S +ICD K ++ + LT L+ +I D ++
Sbjct: 163 YDTLGAEAIIYVINRADISVVICDTPQKATMLIENVEKDLTPGLKTVILM--DPFDDDLM 220
Query: 249 SEGLSSNCKIASFDEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIVA 308
G ++ S + E LGKE+ +P P+ ++V+ +TSG+TG PKG M+TH NIV+
Sbjct: 221 KRGEKCGIEMLSLHDAENLGKENFKKPMPPNPEDLSVICFTSGTTGDPKGAMLTHQNIVS 280
Query: 309 TTAAVMTVIPNLGS---KDVYLAYLPLAHVFEMAAESVMLAAGVAIGY--GSPMTLTDTS 363
AA + + + +DV ++YLPLAH+FE + V+ + G IG+ G L D
Sbjct: 281 NMAAFLKFLEPIFQPTPEDVTISYLPLAHMFERLVQGVIFSCGGKIGFFQGDIRLLPD-- 338
Query: 364 NKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAVK 423
D+ LKPT+ VP +++R+ D V E K L K L +A + V+
Sbjct: 339 ---------DMKALKPTVFPTVPRLLNRVYDKVQN--EAKTPLKKFLLNLAIISKFNEVR 387
Query: 424 GSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIGQGY 483
+ +WD ++F KI++ LGG +R M+ G AP+S F +G + + Y
Sbjct: 388 NGIIR----RNSLWDKLVFSKIQSSLGGKVRLMITGAAPISTPVLTFFRAAMGCWVFEAY 443
Query: 484 GLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFSV 543
G TE AG + + D++ G VG P+ C ++KL + Y S + GE+ + G +V
Sbjct: 444 GQTECTAGCSITSPGDWTAGHVGTPVSCNFVKLEDVADMNYFSVNN---EGEICIKGNNV 500
Query: 544 TAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYISLG 603
GY K+ +KT EV +D+ G W +TGDIGR+ P+G L+IIDRKK+I KL GEYI+
Sbjct: 501 FKGYLKDPEKTQEV--LDKDG--WLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPE 556
Query: 604 KVEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNKPEA 663
K+E + + + VH + S+ + +VV +SL +A + G++ F +LC
Sbjct: 557 KIENVYSRSRPILQVFVHGESLRSFLIGVVVPDPESLPSFAAKIGVK-GSFEELCQNQCV 615
Query: 664 VTEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
+L+ + K K L+ E I + +P++ E+GL+T LK K
Sbjct: 616 KKAILEDLQKVGKEGGLKSFEQVKSIFVHPEPFSIENGLLTPTLKAK 662
>LCF4_YEAST (P47912) Long-chain-fatty-acid--CoA ligase 4 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 4) (Fatty acid
activator 4)
Length = 694
Score = 301 bits (772), Expect = 3e-81
Identities = 211/603 (34%), Positives = 312/603 (50%), Gaps = 45/603 (7%)
Query: 131 LGEYEWETYGEVFARVSNFASGLLKLG--HDIDSHVAIFSDTRAEWFIALQGCFRQNITV 188
L Y TY E+ + + GL+K+G + ++ IF+ T +W GC Q I V
Sbjct: 94 LTPYITMTYEEMICVMHDIGRGLIKIGVKPNGENKFHIFASTSHKWMKTFLGCMSQGIPV 153
Query: 189 VTIYASLGEDALIHSLNETQVSTLICDVKLLNKLDAIRSKLTSLQNIIYFE----DDSKE 244
VT Y +LGE LIHS+ ET + D +LL+KL +++ +I+ E D ++
Sbjct: 154 VTAYDTLGESGLIHSMVETDSVAIFTDNQLLSKLAVPLKTAKNVKFVIHNEPIDPSDKRQ 213
Query: 245 EHTFSEGLSS----------NCKIASFDEVEKLGKESP--VEPSLPSKNAVAVVMYTSGS 292
+ + KI SFDE+ ++GK++ VE P A +MYTSGS
Sbjct: 214 NGKLYKAAKDAVDKIKEVRPDIKIYSFDEIIEIGKKAKDEVELHFPKPEDPACIMYTSGS 273
Query: 293 TGLPKGVMITHGNIVATTAAV-MTVIPNLGSKDVYLAYLPLAHVFEMAAESVMLAAGVAI 351
TG PKGV++TH NIVA V VI +G D +A+LPLAH+FE+ E +
Sbjct: 274 TGTPKGVVLTHYNIVAGIGGVGHNVIGWIGPTDRIIAFLPLAHIFELTFEFEAFYWNGIL 333
Query: 352 GYGSPMTLTDTSNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLF 411
GY + TLT TS + +GD+ KPT++ V A+ + +R G++ K+ E G ++ LF
Sbjct: 334 GYANVKTLTPTSTR---NCQGDLMEFKPTVMVGVAAVWETVRKGILAKINELPGWSQTLF 390
Query: 412 QIAY--KRRLAAVKGSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQ 469
Y K R G G +IFK+IR GGNLRF+L GG+ +S D+Q+
Sbjct: 391 WTVYALKERNIPCSGLLSG-----------LIFKRIREATGGNLRFILNGGSAISIDAQK 439
Query: 470 FINICVGAPIGQGYGLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDK 529
F++ + P+ GYGLTE A A E + + G G + KLV E+ GY + +
Sbjct: 440 FLSNLL-CPMLIGYGLTEGVANACVLEPEHFDYGIAGDLVGTITAKLVDVEDLGYFAKNN 498
Query: 530 PMPRGEVVVGGFSVTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKK 589
+GE++ G + + Y+KN ++T F D WF TGDI + P G ++IIDRKK
Sbjct: 499 ---QGELLFKGAPICSEYYKNPEETAAAFTDDG----WFRTGDIAEWTPKGQVKIIDRKK 551
Query: 590 DIVKLQHGEYISLGKVEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGI 649
++VK +GEYI+L K+E+ S YV +I V+AD V +VV + L K A E GI
Sbjct: 552 NLVKTLNGEYIALEKLESIYRSNPYVQNICVYADENKVKPVGIVVPNLGHLSKLAIELGI 611
Query: 650 EY--KDFPDLCNKPEAVTEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAAL 707
+D ++ + V + + AK+ L E+ I + WTPE+GLVT+A
Sbjct: 612 MVPGEDVESYIHEKKLQDAVCKDMLSTAKSQGLNGIELLCGIVFFEEEWTPENGLVTSAQ 671
Query: 708 KLK 710
KLK
Sbjct: 672 KLK 674
>LCF5_MOUSE (Q8JZR0) Long-chain-fatty-acid--CoA ligase 5 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 5) (LACS 5)
Length = 683
Score = 298 bits (763), Expect = 4e-80
Identities = 190/587 (32%), Positives = 300/587 (50%), Gaps = 37/587 (6%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGHDI--DSHVAIFSDTRAEWFIALQGCFRQNITVVTI 191
Y+W +Y +V R S LL G+ D V IF+ R EW I+ C+ ++ V +
Sbjct: 103 YKWISYKQVSDRAEYLGSCLLHKGYKSSQDQFVGIFAQNRPEWVISELACYTYSMVAVPL 162
Query: 192 YASLGEDALIHSLNETQVSTLICDV--KLLNKLDAIRSKLT-SLQNIIYFEDDSKEEHTF 248
Y +LG +A+I +N + +ICD K ++ + LT L+ II D ++
Sbjct: 163 YDTLGTEAIIFVINRADIPVVICDTPQKATMLVENVEKGLTPGLKTIILM--DPFDDDLM 220
Query: 249 SEGLSSNCKIASFDEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLPKGVMITHGNIVA 308
G ++ S + E +GKE+ +P P ++V+ +TSG+TG PKG M+TH N+V+
Sbjct: 221 KRGEKCGVEMLSLHDAENIGKENFKKPVPPKPEDLSVICFTSGTTGDPKGAMLTHENVVS 280
Query: 309 TTAAVMTVIPNL---GSKDVYLAYLPLAHVFEMAAESVMLAAGVAIGY--GSPMTLTDTS 363
AA + + + S DV ++YLPLAH+FE + ++ + G IG+ G L D
Sbjct: 281 NMAAFLKFLEPIFQPTSDDVTISYLPLAHMFERLVQGILFSCGGKIGFFQGDIRLLPD-- 338
Query: 364 NKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAVK 423
D+ LKPT+ VP +++R+ D V E K L K L +A + VK
Sbjct: 339 ---------DMKALKPTVFPTVPRLLNRVYDKVQN--EAKTPLKKFLLNLAIISKFNEVK 387
Query: 424 GSWLGAWGVEKLVWDTIIFKKIRTVLGGNLRFMLCGGAPLSGDSQQFINICVGAPIGQGY 483
+ +WD ++F KI+ LGG +R M+ G AP+S F +G + + Y
Sbjct: 388 NGIIR----RDSLWDKLVFSKIQGSLGGKVRLMITGAAPISTPVLTFFRAAMGCWVFEAY 443
Query: 484 GLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFSV 543
G TE G + + D++ G VG P+ C ++KL + Y S + GE+ + G +V
Sbjct: 444 GQTECTGGCSITSPGDWTAGHVGTPVACNFVKLEDVADMNYFSVNN---EGEICIKGNNV 500
Query: 544 TAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYISLG 603
GY K+ +KT EV +D+ G W +TGDIGR+ P+G L+I+DRKK+I KL GEYI+
Sbjct: 501 FKGYLKDPEKTQEV--LDKDG--WLHTGDIGRWLPNGTLKIVDRKKNIFKLAQGEYIAPE 556
Query: 604 KVEAALASGDYVDSIMVHADPFHSYCVALVVASHQSLEKWAQETGIEYKDFPDLCNKPEA 663
K+E + V + VH + S+ + +VV SL +A + G++ F +LC
Sbjct: 557 KIENVYSRSRPVLQVFVHGESLRSFLIGVVVPDPDSLPSFAAKIGVK-GSFEELCKNQCV 615
Query: 664 VTEVLQSISKAAKAAKLQKTEVPAKIKLLADPWTPESGLVTAALKLK 710
+L+ + K K L+ E I + +P+T E+GL+T LK K
Sbjct: 616 KEAILEDLQKIGKEGGLKSFEQVKSIFVHPEPFTIENGLLTPTLKAK 662
>LCF3_YEAST (P39002) Long-chain-fatty-acid--CoA ligase 3 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 3) (Fatty acid
activator 3)
Length = 694
Score = 294 bits (753), Expect = 5e-79
Identities = 208/622 (33%), Positives = 322/622 (51%), Gaps = 44/622 (7%)
Query: 110 RKLIGKEFVTSSDGRKFEKVHLGEYEWETYGEVFARVSNFASGLLKLG--HDIDSHVAIF 167
RK+ GK+ +E + Y+ TY E+ + + GL K+G + + IF
Sbjct: 76 RKVDGKDKSIEKTWLYYE---MSPYKMMTYQELIWVMHDMGRGLAKIGIKPNGEHKFHIF 132
Query: 168 SDTRAEWFIALQGCFRQNITVVTIYASLGEDALIHSLNETQVSTLICDVKLLNKLDAIRS 227
+ T +W GC Q I VVT Y +LGE LIHS+ ET+ + + D +LL K+
Sbjct: 133 ASTSHKWMKIFLGCISQGIPVVTAYDTLGESGLIHSMVETESAAIFTDNQLLAKMIVPLQ 192
Query: 228 KLTSLQNIIYFE----DDSKEEHTFSEGLSS----------NCKIASFDEVEKLGKESPV 273
++ +I+ E +D ++ + + KI SF+EV K+GK+S
Sbjct: 193 SAKDIKFLIHNEPIDPNDRRQNGKLYKAAKDAINKIREVRPDIKIYSFEEVVKIGKKSKD 252
Query: 274 EPSL--PSKNAVAVVMYTSGSTGLPKGVMITHGNIVATTAAV-MTVIPNLGSKDVYLAYL 330
E L P +A +MYTSGS PKGV++TH NIV+ A V V +GS D L++L
Sbjct: 253 EVKLHPPEPKDLACIMYTSGSISAPKGVVLTHYNIVSGIAGVGHNVFGWIGSTDRVLSFL 312
Query: 331 PLAHVFEMAAESVMLAAGVAIGYGSPMTLTDTSNKVKKGTKGDVTVLKPTLLTAVPAIID 390
PLAH+FE+ E +GYGS TLT+TS + KGD+ KPT++ V A+ +
Sbjct: 313 PLAHIFELVFEFEAFYWNGILGYGSVKTLTNTSTR---NCKGDLVEFKPTIMIGVAAVWE 369
Query: 391 RIRDGVVKKVEEKGGLAKNLFQIAYKRRLAAVKGSWLGAWGVEKLVWDTIIFKKIRTVLG 450
+R +++K+ + + + +F AY + +V + ++FKK+R G
Sbjct: 370 TVRKAILEKISDLTPVLQKIFWSAYSMKEKSVPCTGF---------LSRMVFKKVRQATG 420
Query: 451 GNLRFMLCGGAPLSGDSQQFINICVGAPIGQGYGLTETFAGAAFSEADDYSVGRVGPPLP 510
G+L++++ GG+ +S D+Q+F +I + P+ GYGLTET A A E D + G VG +
Sbjct: 421 GHLKYIMNGGSAISIDAQKFFSIVL-CPMIIGYGLTETVANACVLEPDHFEYGIVGDLVG 479
Query: 511 CCYIKLVSWEEGGYLSSDKPMPRGEVVVGGFSVTAGYFKNQDKTDEVFKVDEKGVRWFYT 570
KLV ++ GY + + +GE+++ G V + Y+KN +T F D WF T
Sbjct: 480 SVTAKLVDVKDLGYYAKNN---QGELLLKGAPVCSEYYKNPIETAVSFTYDG----WFRT 532
Query: 571 GDIGRFHPDGCLEIIDRKKDIVKLQHGEYISLGKVEAALASGDYVDSIMVHADPFHSYCV 630
GDI + P G L+IIDR+K++VK +GEYI+L K+E+ S YV +I V+AD V
Sbjct: 533 GDIVEWTPKGQLKIIDRRKNLVKTLNGEYIALEKLESVYRSNSYVKNICVYADESRVKPV 592
Query: 631 ALVVASHQSLEKWAQETGIEYK--DFPDLCNKPEAVTEVLQSISKAAKAAKLQKTEVPAK 688
+VV + L K+A + I K D + + V + + AK+ L E+
Sbjct: 593 GIVVPNPGPLSKFAVKLRIMKKGEDIENYIHDKALRNAVFKEMIATAKSQGLVGIELLCG 652
Query: 689 IKLLADPWTPESGLVTAALKLK 710
I + WTPE+G VT+A KLK
Sbjct: 653 IVFFDEEWTPENGFVTSAQKLK 674
>LCF2_YEAST (P39518) Long-chain-fatty-acid--CoA ligase 2 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 2) (Fatty acid
activator 2)
Length = 744
Score = 216 bits (550), Expect = 2e-55
Identities = 185/620 (29%), Positives = 289/620 (45%), Gaps = 73/620 (11%)
Query: 134 YEWETYGEVFARVSNFASGLLKLGHDI--------DSHVAIFSDTRAEWFIALQGCFRQN 185
+ +E+Y V R N SG+L L + D VAI S EW + C +
Sbjct: 128 FRFESYSTVSKRCHNIGSGILSLVNTKRKRPLEANDFVVAILSHNNPEWILTDLACQAYS 187
Query: 186 ITVVTIYASLGEDALIHSLNETQVSTLICDVKLLNKLDAIRSKLTSLQNIIYFEDDSKEE 245
+T +Y +LG + + LN T+ LI + + + + + ++ ++ + +E
Sbjct: 188 LTNTALYETLGPNTSEYILNLTEAPILIFAKSNMYHVLKMVPDMKFVNTLVCMDELTHDE 247
Query: 246 -HTFSEGL------SSNCKIASF--DEVEKLGKESPVEPSLPSKNAVAVVMYTSGSTGLP 296
+E L S N KI F ++VE++G + + P+ +++ + +TSG+TGLP
Sbjct: 248 LRMLNESLLPVKCNSLNEKITFFSLEQVEQVGCFNKIPAIPPTPDSLYTISFTSGTTGLP 307
Query: 297 KGVMITHGNI---VATTAAVMTVIPNLGSKDVY-LAYLPLAHVFEMAAESVMLAAGVAIG 352
KGV ++H NI +A + + P+ ++ +Y + +LPLAH+FE + LA G IG
Sbjct: 308 KGVEMSHRNIASGIAFAFSTFRIPPDKRNQQLYDMCFLPLAHIFERMVIAYDLAIGFGIG 367
Query: 353 Y---GSPMTLTDTSNKVKKGTKGDVTVLKPTLLTAVPAIIDRIRDGVVKKVEEKGGLAKN 409
+ P L + D+ +LKP + VP I+ R G+ K L K+
Sbjct: 368 FLHKPDPTVLVE-----------DLKILKPYAVALVPRILTRFEAGI------KNALDKS 410
Query: 410 LFQIAYKRRLAAVKGSWLGAWG------VEKLVWDTIIFKKIRTVLG-GNLRFMLCGGAP 462
Q + K + A G + LV+ ++ KIR LG N F++ G AP
Sbjct: 411 TVQRNVANTILDSKSARFTARGGPDKSIMNFLVYHRVLIDKIRDSLGLSNNSFIITGSAP 470
Query: 463 LSGDSQQFINICVGAPIGQGYGLTETFAGAAFSEADDYSVGRVGPPLPCCYIKLVSWEEG 522
+S D+ F+ + I QGYGLTETFAG SE + VG G +L S E
Sbjct: 471 ISKDTLLFLRSALDIGIRQGYGLTETFAGVCLSEPFEKDVGSCGAIGISAECRLKSVPEM 530
Query: 523 GYLSSDKPMPRGEVVVGGFSVTAGYFKNQDKTDEVFKVDEKGVRWFYTGDIGRFHPDGCL 582
GY +DK + +GE+ + G V YFKN ++T + VD+ G WF TGD+ G +
Sbjct: 531 GY-HADKDL-KGELQIRGPQVFERYFKNPNETSKA--VDQDG--WFSTGDVAFIDGKGRI 584
Query: 583 EIIDRKKDIVKLQHGEYISLGKVE-AALASGDYVDSIMVHADPFHSYCVALV-------- 633
+IDR K+ KL HGEYI+ K+E L+S Y+ I V DP ++ V +V
Sbjct: 585 SVIDRVKNFFKLAHGEYIAPEKIENIYLSSCPYITQIFVFGDPLKTFLVGIVGVDVDAAQ 644
Query: 634 ---VASHQSLEKWAQETGIEYKDFPDLCNKPEAVTEVLQSISKAAKAAKLQKTEVPAKIK 690
A H ++ W +E +E +L + E L I+K LQ E IK
Sbjct: 645 PILAAKHPEVKTWTKEVLVE-----NLNRNKKLRKEFLNKINKCTDG--LQGFEKLHNIK 697
Query: 691 LLADPWTPESGLVTAALKLK 710
+ +P T E +VT K+K
Sbjct: 698 VGLEPLTLEDDVVTPTFKIK 717
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,270,787
Number of Sequences: 164201
Number of extensions: 3763848
Number of successful extensions: 9827
Number of sequences better than 10.0: 226
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 48
Number of HSP's that attempted gapping in prelim test: 9077
Number of HSP's gapped (non-prelim): 494
length of query: 755
length of database: 59,974,054
effective HSP length: 118
effective length of query: 637
effective length of database: 40,598,336
effective search space: 25861140032
effective search space used: 25861140032
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 70 (31.6 bits)
Medicago: description of AC148718.8