BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148654.3 - phase: 0 /pseudo
(719 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CYPC_STRCO (P23154) Putative polyketide cyclase (WhiE ORF VI) 45 5e-04
CYPC_STRCN (Q02572) Putative polyketide cyclase 45 7e-04
YBG7_PSEAE (O68560) Hypothetical UPF0083 protein PA4767 37 0.18
RED1_YEAST (P14291) RED1 protein 35 0.53
HSF1_MOUSE (P38532) Heat shock factor protein 1 (HSF 1) (Heat sh... 35 0.53
TPM_PERVI (Q9GZ70) Tropomyosin 34 1.5
SYIC_SCHPO (O13651) Isoleucyl-tRNA synthetase, cytoplasmic (EC 6... 34 1.5
GOA5_HUMAN (Q8TBA6) Golgi autoantigen, golgin subfamily A member... 33 2.0
SRB9_YEAST (P38931) Suppressor of RNA polymerase B SRB9 (SCA1 pr... 33 2.6
RP54_AZOCA (P33984) RNA polymerase sigma-54 factor 33 3.4
PLA6_HUMAN (Q9Y2H5) Pleckstrin homology domain-containing protei... 33 3.4
ULP2_YEAST (P40537) Ubiquitin-like-specific protease 2 (EC 3.4.2... 32 4.5
PURL_STRCO (Q9RKK5) Phosphoribosylformylglycinamidine synthase I... 32 4.5
KUP1_LACLA (Q9CHU5) Probable potassium transport system protein ... 32 4.5
PTP3_ENCCU (Q8MTP3) Polar tube protein 3 precursor 32 7.6
PCNA_MAIZE (Q43266) Proliferating cell nuclear antigen (PCNA) 32 7.6
CYPC_STRHA (Q05368) Putative polyketide cyclase (Fragment) 32 7.6
THYG_BOVIN (P01267) Thyroglobulin precursor 31 10.0
TCMN_STRGA (P16559) Multifunctional cyclase-dehydratase-3-O-meth... 31 10.0
CCA_SCHMA (O02197) Circulating cathodic antigen (CCA) 31 10.0
>CYPC_STRCO (P23154) Putative polyketide cyclase (WhiE ORF VI)
Length = 159
Score = 45.4 bits (106), Expect = 5e-04
Identities = 33/142 (23%), Positives = 68/142 (47%), Gaps = 9/142 (6%)
Query: 363 ITVKAPVRDVWNVMSSYETLPEIVPNLAISKILSRDNNKVRI---LQEGCKGLLYMVLHA 419
IT+ AP+ VWN+ + E P + A ++L RD++KV + G ++ +
Sbjct: 9 ITIAAPMELVWNMTNDIEKWPGLFSEYASVEVLGRDDDKVTFRLTMHPDADGKVWSWVSE 68
Query: 420 RVVLDLCEQLEQEISFEQAE-GDFDSFHGKWTFEQLGNHHTLLKYSVDSKMRRDTFLSEA 478
RV + + + + ++ E G F + W + + T+++++ D M+ D + +A
Sbjct: 69 RV----ADPVTRTVRAQRVETGPFQYMNIVWEYAETA-EGTVMRWTQDFAMKPDAPVDDA 123
Query: 479 IMEEVIYEDLPSNLCAIRDYVE 500
M + I + + + IRD +E
Sbjct: 124 WMTDNINRNSRTQMALIRDRIE 145
>CYPC_STRCN (Q02572) Putative polyketide cyclase
Length = 152
Score = 45.1 bits (105), Expect = 7e-04
Identities = 35/142 (24%), Positives = 68/142 (47%), Gaps = 9/142 (6%)
Query: 363 ITVKAPVRDVWNVMSSYETLPEIVPNLAISKILSRDNNKVRI---LQEGCKGLLYMVLHA 419
IT+ APV VW++ + E PE+ A ++LSR+ N V + G ++ +
Sbjct: 9 ITIAAPVDLVWDMTNDLERWPELFSEYASCEVLSREANTVTFRLTMHPDENGKVWSWVSE 68
Query: 420 RVVLDLCEQLEQEISFEQAE-GDFDSFHGKWTFEQLGNHHTLLKYSVDSKMRRDTFLSEA 478
R ++ + + + E G F+ + W +E+ + T + ++ D M+ D + +A
Sbjct: 69 RT----ADREKLVVRARRVETGPFEYMNIVWEYEETPD-GTRMHWTQDFAMKPDAPVDDA 123
Query: 479 IMEEVIYEDLPSNLCAIRDYVE 500
M ++I + P + IRD +E
Sbjct: 124 GMTDIINRNSPIQMALIRDRIE 145
>YBG7_PSEAE (O68560) Hypothetical UPF0083 protein PA4767
Length = 144
Score = 37.0 bits (84), Expect = 0.18
Identities = 23/88 (26%), Positives = 39/88 (44%), Gaps = 4/88 (4%)
Query: 368 PVRDVWNVMSSYETLPEIVPNLAISKILSRDNNKVRILQEGCKGLLYMVLHARVVLDLCE 427
P R ++++++ + PE +P + S++L + +R KG L R VL
Sbjct: 14 PARALFDLVNDVKRYPEFLPWCSASQVLEESESLMRAELTVAKGSLSQRFTTRNVLVPGA 73
Query: 428 QLEQEISFEQAEGDFDSFHGKWTFEQLG 455
+E + G F HG W F+ LG
Sbjct: 74 SIEMNLE----NGPFTELHGVWQFKALG 97
>RED1_YEAST (P14291) RED1 protein
Length = 827
Score = 35.4 bits (80), Expect = 0.53
Identities = 34/162 (20%), Positives = 69/162 (41%), Gaps = 16/162 (9%)
Query: 462 KYSVDSKMRRDTFLSEAIMEEVIYEDLPSNLCAIRDYVENQKASQFLEVCEQNTNSGQQI 521
K S++SK +TF+ +++E+ D N C I D +E+ V + + G
Sbjct: 676 KVSLESKKNNETFVDSSVVEKHTPPDKDCNNCNITDILES------TTVIDLQSPHG--- 726
Query: 522 ILSGSGDDNNSSSADDISDCNVQSSSNQRSRVPGLQRDIEVLKSELLKFVAEYGQEGFMP 581
LS G + ++ + ++ SN+ L R I ++ EL K + + E +
Sbjct: 727 -LSAPGQNTFTNKLQEQIYSSINHFSNE------LVRKISIINQELNKKILKELSEKYQK 779
Query: 582 MRKQLRLHGRVDIEKAITRMGGFRKIATIMNLSLAYKYRKPK 623
+ +L+ + + D + + MG + + + L + R K
Sbjct: 780 LFSELQDNFQNDTNEMLKFMGEIKDMMNLPEDQLVHAIRTRK 821
>HSF1_MOUSE (P38532) Heat shock factor protein 1 (HSF 1) (Heat shock
transcription factor 1) (HSTF 1)
Length = 525
Score = 35.4 bits (80), Expect = 0.53
Identities = 21/72 (29%), Positives = 38/72 (52%), Gaps = 3/72 (4%)
Query: 454 LGNHHTLLK---YSVDSKMRRDTFLSEAIMEEVIYEDLPSNLCAIRDYVENQKASQFLEV 510
L N T+L +SVD+ D F M ++ DL S+L +I++ + Q+ + +E
Sbjct: 391 LDNLQTMLTSHGFSVDTSALLDLFSPSVTMPDMSLPDLDSSLASIQELLSPQEPPRPIEA 450
Query: 511 CEQNTNSGQQII 522
N +SG+Q++
Sbjct: 451 ENSNPDSGKQLV 462
>TPM_PERVI (Q9GZ70) Tropomyosin
Length = 284
Score = 33.9 bits (76), Expect = 1.5
Identities = 41/195 (21%), Positives = 87/195 (44%), Gaps = 20/195 (10%)
Query: 410 KGLLYMVLHARVVLDLCEQLEQEI-SFEQAEGDFDSFHGKWTFEQLGNHHTLLKYSVDSK 468
K ++ M + + LD EQLEQ++ E+A+ + ++++L+K ++ ++
Sbjct: 6 KKMVAMKMEKKNALDRAEQLEQKLRETEEAKAKIED-----------DYNSLVKKNIQTE 54
Query: 469 MRRDTFLSEAIMEEVIYEDLPSNLCAIRDYVENQKASQFLEVCEQNTNSGQQIILSGSGD 528
D ++ + YE + E Q ++ + + E+ ++ + SG
Sbjct: 55 NDYDNCNTQLQDVQAKYERAEKQIQEHEQ--EIQSLTRKISLLEEGIMKAEERFTTASGK 112
Query: 529 DNNSSSADDISDCNVQSSSNQRSRVPGLQRDIEVLKSEL--LKFVAEYGQEGFMPMRKQL 586
+S A D S+ N + N S G I+ L+ +L K++AE + + ++L
Sbjct: 113 LEEASKAADESERNRKVLENLNS---GNDERIDQLEKQLTEAKWIAEEADKKYEEAARKL 169
Query: 587 RLHGRVDIEKAITRM 601
+ VD+E+A R+
Sbjct: 170 AI-TEVDLERAEARL 183
>SYIC_SCHPO (O13651) Isoleucyl-tRNA synthetase, cytoplasmic (EC
6.1.1.5) (Isoleucine--tRNA ligase) (IleRS)
Length = 1064
Score = 33.9 bits (76), Expect = 1.5
Identities = 15/28 (53%), Positives = 19/28 (67%)
Query: 387 PNLAISKILSRDNNKVRILQEGCKGLLY 414
P+L KIL +D+NK IL E C G+LY
Sbjct: 234 PDLQYIKILDKDSNKKYILMESCLGILY 261
>GOA5_HUMAN (Q8TBA6) Golgi autoantigen, golgin subfamily A member 5
(Golgin-84) (RET-fused gene 5 protein) (Ret-II protein)
Length = 731
Score = 33.5 bits (75), Expect = 2.0
Identities = 55/210 (26%), Positives = 94/210 (44%), Gaps = 22/210 (10%)
Query: 395 LSRDNNKVRILQEGCKGLLYMVLHARVVLDLCEQLEQEISFEQAEGDFDSFHGKWTFEQ- 453
+ +D ++ LQ L LH D + EQE S++Q + +F + K E+
Sbjct: 332 IMQDQSEGNSLQNQALQTLQERLHEA---DATLKREQE-SYKQMQSEFAARLNKVEMERQ 387
Query: 454 -LGNHHTLL--KYSVDSKMRRDTFLSEAIMEEVIYEDLPSNLCAIRDYVENQKASQFLEV 510
L TL KYS D K R D + + ++ +L S+ + DY QKA++ L+
Sbjct: 388 NLAEAITLAERKYS-DEKKRVDELQQQVKLYKL---NLESSKQELIDY--KQKATRILQS 441
Query: 511 CEQNTNSGQQIILSGSGD---DNNSSSADDISDCNVQSSSNQRSRVPGLQRDIEVLKSEL 567
E+ NS + GSG D++++S+ ++ + + QR + L I L+SEL
Sbjct: 442 KEKLINS----LKEGSGFEGLDSSTASSMELEELRHEKEM-QREEIQKLMGQIHQLRSEL 496
Query: 568 LKFVAEYGQEGFMPMRKQLRLHGRVDIEKA 597
A+ E + LH ++ +KA
Sbjct: 497 QDMEAQQVNEAESAREQLQDLHDQIAGQKA 526
>SRB9_YEAST (P38931) Suppressor of RNA polymerase B SRB9 (SCA1
protein)
Length = 1420
Score = 33.1 bits (74), Expect = 2.6
Identities = 23/81 (28%), Positives = 40/81 (48%), Gaps = 6/81 (7%)
Query: 254 ERNLLGNQKLPQPEDDLH----KTSLVVNGSSVKKINGSLCETDKLAPGQDKEGL--DTS 307
E L ++K +DL KT L NG S++K+N S+ +T + +KEG
Sbjct: 406 ENELFNDRKQTTVSNDLENSPLKTELEANGRSLEKVNNSVSKTGSVDTLHNKEGTLEQRE 465
Query: 308 ISGSLPASSSELNSNWGIFGK 328
+ +LP+ S+ + +FG+
Sbjct: 466 QNENLPSDKSDSMVDKELFGE 486
>RP54_AZOCA (P33984) RNA polymerase sigma-54 factor
Length = 514
Score = 32.7 bits (73), Expect = 3.4
Identities = 24/94 (25%), Positives = 41/94 (43%), Gaps = 4/94 (4%)
Query: 299 QDKEGLDTSISGSLPASSSELNSNWGIFGKVCSLDKPCVVDEV-HLRRFD---GLLENGG 354
+DK+ D ++ L + ++C +D + D + +RR D GL GG
Sbjct: 231 RDKDRCDPAMQALLDNLELLARHDRNALKRICGVDAEDLADMIGEIRRLDPKPGLAYGGG 290
Query: 355 VHRCVVASITVKAPVRDVWNVMSSYETLPEIVPN 388
V +V + V+ W V + ETLP ++ N
Sbjct: 291 VVHPLVPDVFVREGSDGSWIVELNSETLPRVLVN 324
>PLA6_HUMAN (Q9Y2H5) Pleckstrin homology domain-containing protein
family A member 6 (Phosphoinositol 3-phosphate-binding
protein-3) (PEPP-3)
Length = 1048
Score = 32.7 bits (73), Expect = 3.4
Identities = 29/113 (25%), Positives = 45/113 (39%), Gaps = 14/113 (12%)
Query: 211 RSSSTNLSYEVNVIPRFNFPAIFLERIVRSDLPVNLRALAYRVERNLLGNQKLPQPE--- 267
RS S + P+ ER+ + AY + R++ + P PE
Sbjct: 458 RSPSQGSYSRARIYSPVRSPSARFERLPPRSEDIYADPAAYVMRRSISSPKVPPYPEVFR 517
Query: 268 DDLHKTSLVVNGSSVKKINGSLCETDKLAPGQD---------KEGLDTSISGS 311
D LH L N K+ G LCE +K+ QD KE L++++ G+
Sbjct: 518 DSLHTYKL--NEQDTDKLLGKLCEQNKVVREQDRLVQQLRAEKESLESALMGT 568
>ULP2_YEAST (P40537) Ubiquitin-like-specific protease 2 (EC
3.4.22.-)
Length = 1034
Score = 32.3 bits (72), Expect = 4.5
Identities = 18/50 (36%), Positives = 24/50 (48%)
Query: 495 IRDYVENQKASQFLEVCEQNTNSGQQIILSGSGDDNNSSSADDISDCNVQ 544
I D++ N FL V N+ S +QI LS + DNN S C+ Q
Sbjct: 45 IVDHLNNASTKDFLPVLSMNSESKRQIELSDNDVDNNDEGEGVNSGCSDQ 94
>PURL_STRCO (Q9RKK5) Phosphoribosylformylglycinamidine synthase II
(EC 6.3.5.3) (FGAM synthase II)
Length = 752
Score = 32.3 bits (72), Expect = 4.5
Identities = 19/64 (29%), Positives = 34/64 (52%), Gaps = 6/64 (9%)
Query: 450 TFEQLGNHHTLLKYSVDSKMRRDTFLSEAIMEEVIYEDLPSNLCAIRDYVENQKASQFLE 509
T E N ++ ++D RD+ LS EE++ + +CA+ VE +K +FLE
Sbjct: 291 TSELASNGSGGMRVTLDDVPLRDSTLSP---EEILMSESQERMCAV---VEPEKVDRFLE 344
Query: 510 VCEQ 513
+C++
Sbjct: 345 ICDK 348
>KUP1_LACLA (Q9CHU5) Probable potassium transport system protein
kup1
Length = 670
Score = 32.3 bits (72), Expect = 4.5
Identities = 26/96 (27%), Positives = 46/96 (47%), Gaps = 7/96 (7%)
Query: 173 VLDLQELLNSEW-DRELHFSMVDGDFKKFEGKWSVKSGTRSSSTNLSYEVNVIPRFNFPA 231
V+ L ++ EW DR + +S++D KK + W VK YEV+++
Sbjct: 487 VVYLTAKMDKEWIDRSILYSILDKRPKKAKVYWFVKVNVTDEPYTSEYEVDMLGTDFIVC 546
Query: 232 I--FLERIVRSDLPVNLRALAYRVERNLLGNQKLPQ 265
+ +L +R ++P LR + NL+ + +LPQ
Sbjct: 547 VNLYLGFHMRQEIPRYLRTIV----TNLMESGRLPQ 578
>PTP3_ENCCU (Q8MTP3) Polar tube protein 3 precursor
Length = 1256
Score = 31.6 bits (70), Expect = 7.6
Identities = 44/191 (23%), Positives = 72/191 (37%), Gaps = 19/191 (9%)
Query: 452 EQLGNHHTLLKYSVDSKMRRDTFLSEAIMEEVIYEDLPSNLCAIRDYVENQKA----SQF 507
E L N T LK K FLS+ + E+ I + N ++ V+ QK S
Sbjct: 475 ENLENVKTQLKPG-GRKTNALNFLSKVLTEKAIKDVNQRNEEMQKEAVKAQKEANENSAV 533
Query: 508 LEVCEQNTNSGQQIILSGSGDDNNSS---SADDISDCNVQSSSNQRSRVPGLQRDIEVLK 564
E + +Q I++ G S S DIS+ +R+ L+ E K
Sbjct: 534 KSAEEHLAKAAEQQIINNEGKSVFSKVLESTGDISEAAKAREEAERAMAETLRHQREARK 593
Query: 565 SELLKFVAEYGQEGFMPMRKQLRLHGRVDIEKAITRMGGFRKIATIMNLSLAYKYRKPKG 624
+E++K + G + + +E T G + + +N L KY +
Sbjct: 594 AEVMKMLGPMGMNSSV----------KEAVEHMATAHAGLNVVGSDLN-ELNKKYEEEVA 642
Query: 625 YWDNLENLQDE 635
+ N+E L E
Sbjct: 643 HQSNIEELLRE 653
>PCNA_MAIZE (Q43266) Proliferating cell nuclear antigen (PCNA)
Length = 263
Score = 31.6 bits (70), Expect = 7.6
Identities = 16/71 (22%), Positives = 34/71 (47%)
Query: 504 ASQFLEVCEQNTNSGQQIILSGSGDDNNSSSADDISDCNVQSSSNQRSRVPGLQRDIEVL 563
+S+F+ +C+ ++ G +++S + + S++ +I N+ NQ P IE+
Sbjct: 141 SSEFMRICKDLSSIGDTVVISVTKEGVKFSTSGEIGSANIVCRQNQTIDKPEEATIIEMQ 200
Query: 564 KSELLKFVAEY 574
+ L F Y
Sbjct: 201 EPVSLTFALRY 211
>CYPC_STRHA (Q05368) Putative polyketide cyclase (Fragment)
Length = 109
Score = 31.6 bits (70), Expect = 7.6
Identities = 22/95 (23%), Positives = 44/95 (46%), Gaps = 8/95 (8%)
Query: 363 ITVKAPVRDVWNVMSSYETLPEIVPNLAISKILSRDNNKVRI---LQEGCKGLLYMVLHA 419
I + AP+ VW++ + E P + A ++IL R+ ++VR + +G ++ +
Sbjct: 9 IVIAAPLDLVWDMTNDVENWPRLFSEYASAEILEREGDRVRFRLTMHPDDEGRVWSWVSE 68
Query: 420 RVVLDLCEQLEQEISFEQAE-GDFDSFHGKWTFEQ 453
RV ++ + + E G F +W +EQ
Sbjct: 69 RV----ADRASLTVRAHRVETGPFQFMDIQWVYEQ 99
>THYG_BOVIN (P01267) Thyroglobulin precursor
Length = 2769
Score = 31.2 bits (69), Expect = 10.0
Identities = 20/69 (28%), Positives = 32/69 (45%), Gaps = 11/69 (15%)
Query: 306 TSISGSLPASSSELNSNWGIFGKVCSLDKPCVVDEVHLRRFDG-------LLENGGVHRC 358
T I G L + SSEL+ NWG+ +V +L + H++ F G + GG
Sbjct: 2338 TGIFGFLSSGSSELSGNWGLLDQVVAL----TWVQTHIQAFGGDPRRVTLAADRGGADIA 2393
Query: 359 VVASITVKA 367
+ +T +A
Sbjct: 2394 SIHLVTTRA 2402
>TCMN_STRGA (P16559) Multifunctional cyclase-dehydratase-3-O-methyl
transferase tcmN
Length = 494
Score = 31.2 bits (69), Expect = 10.0
Identities = 15/39 (38%), Positives = 22/39 (55%)
Query: 362 SITVKAPVRDVWNVMSSYETLPEIVPNLAISKILSRDNN 400
SI V AP VW+V + E PE+ A ++IL +D +
Sbjct: 8 SIVVNAPFELVWDVTNDIEAWPELFSEYAEAEILRQDGD 46
>CCA_SCHMA (O02197) Circulating cathodic antigen (CCA)
Length = 347
Score = 31.2 bits (69), Expect = 10.0
Identities = 27/94 (28%), Positives = 42/94 (43%), Gaps = 10/94 (10%)
Query: 467 SKMRRDTFLSEAIMEEVIYEDLPSNLCAIRDYVENQKASQFLEVCEQNTNSGQQIILSGS 526
SK+ R T+ E +++ VI D N C I+ + K+ NT S I
Sbjct: 118 SKLMR-TYQVEHLVQSVINPDNNENRC-IKSFYPGPKSV--------NTPSTNSIDSQSV 167
Query: 527 GDDNNSSSADDISDCNVQSSSNQRSRVPGLQRDI 560
+NS D+I+D QS + Q + V + RD+
Sbjct: 168 SQKSNSGKVDEITDGISQSLTEQFTTVASIVRDV 201
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.137 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,074,782
Number of Sequences: 164201
Number of extensions: 3543697
Number of successful extensions: 8683
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 8674
Number of HSP's gapped (non-prelim): 22
length of query: 719
length of database: 59,974,054
effective HSP length: 118
effective length of query: 601
effective length of database: 40,598,336
effective search space: 24399599936
effective search space used: 24399599936
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 69 (31.2 bits)
Medicago: description of AC148654.3