BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148653.4 - phase: 0
(427 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
GLTR_ARATH (Q9FWA4) Probable glycosyltransferase At3g02350 (EC 2... 338 2e-92
QUA1_ARATH (Q9LSG3) Glycosyltransferase QUASIMODO1 (EC 2.4.1.-) 335 2e-91
GSPA_BACSU (P25148) General stress protein A 53 1e-06
UGGG_SCHPO (Q09140) UDP-glucose:glycoprotein glucosyltransferase... 38 0.044
UGG2_HUMAN (Q9NYU1) UDP-glucose:glycoprotein glucosyltransferase... 37 0.074
UGGG_DROME (Q09332) UDP-glucose:glycoprotein glucosyltransferase... 37 0.097
RFAJ_ECOLI (P27129) Lipopolysaccharide 1,2-glucosyltransferase (... 36 0.17
Y258_HAEIN (P43974) Putative glycosyl transferase HI0258 (EC 2.-... 35 0.48
VMT8_MYXVL (P22611) M-T8 protein 33 1.1
LAGE_MOUSE (Q9Z1M7) Glycosyltransferase-like protein LARGE (EC 2... 32 2.4
LAGE_HUMAN (O95461) Glycosyltransferase-like protein LARGE (EC 2... 32 2.4
PURA_DROME (Q9Y0Y2) Adenylosuccinate synthetase (EC 6.3.4.4) (IM... 31 6.9
PEX6_PICPA (P33289) Peroxisomal biogenesis factor 6 (Peroxin-6) ... 31 6.9
MFD_RICPR (O05955) Transcription-repair coupling factor (TRCF) 30 9.1
>GLTR_ARATH (Q9FWA4) Probable glycosyltransferase At3g02350 (EC
2.4.1.-)
Length = 561
Score = 338 bits (867), Expect = 2e-92
Identities = 164/371 (44%), Positives = 234/371 (62%), Gaps = 19/371 (5%)
Query: 55 SNIASNSIPEQLHCLDLTLANEHANNAAARLRLPSADLVPALVDNSYFHFVLASDNVLAA 114
S I++ S+P+ LHCL + L E +N + A PA D + +H+ + SDNV+A
Sbjct: 206 SLISAKSVPKSLHCLAMRLVGERISNPE---KYKDAPPDPAAEDPTLYHYAIFSDNVIAV 262
Query: 115 SVVAKSLVQNSLQPWKVVLHIITDKKTYNSMQAWFSLHSLSP-AIIEVKSLQDFDWFREG 173
SVV +S+V N+ +PWK V H++TD+ +M+ WF + L A +E+KS++DF +
Sbjct: 263 SVVVRSVVMNAEEPWKHVFHVVTDRMNLAAMKVWFKMRPLDRGAHVEIKSVEDFKFLNSS 322
Query: 174 KVSLLEAMEKDQHVRSRFRGGSSAIISNTAEKPEDIAAKLKALSPTYSSVMNHIRIYIPE 233
+L +E + + F N AE + LK +P Y S++NH+R Y+PE
Sbjct: 323 YAPVLRQLESAKLQKFYFE--------NQAENATKDSHNLKFKNPKYLSMLNHLRFYLPE 374
Query: 234 LFPSLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCKGKDKRVMSKMLKSYLNFSH 293
++P L+K++FLD+D+VVQ D++ LW I+++GKVNGAVETC G R YLNFSH
Sbjct: 375 MYPKLNKILFLDDDVVVQKDVTGLWKINLDGKVNGAVETCFGSFHRY-----GQYLNFSH 429
Query: 294 PLISKNFDPNECAWAYGMNILDLEAWRKTNISYTYHYWVEQNIKSDLSLWHLGTLPPGLI 353
PLI +NF+P+ CAWA+GMNI DL AWR+ + YHYW QN+ D +LW LGTLPPGLI
Sbjct: 430 PLIKENFNPSACAWAFGMNIFDLNAWRREKCTDQYHYW--QNLNEDRTLWKLGTLPPGLI 487
Query: 354 AFHGNVHTIDPFWHMLGLGYQENTNLVDAKNAGVVHFNGWAKPWLDIAFPQLKPLWTKYV 413
F+ ++D WH+LGLGY ++ + +NAGV+H+NG KPWLDIA Q K LWTKYV
Sbjct: 488 TFYSKTKSLDKSWHVLGLGYNPGVSMDEIRNAGVIHYNGNMKPWLDIAMNQYKSLWTKYV 547
Query: 414 DFSDNFIESCH 424
D F++ C+
Sbjct: 548 DNEMEFVQMCN 558
>QUA1_ARATH (Q9LSG3) Glycosyltransferase QUASIMODO1 (EC 2.4.1.-)
Length = 559
Score = 335 bits (858), Expect = 2e-91
Identities = 163/370 (44%), Positives = 229/370 (61%), Gaps = 16/370 (4%)
Query: 55 SNIASNSIPEQLHCLDLTLANEHANNAAARLRLPSADLVPALVDNSYFHFVLASDNVLAA 114
S IA+ SIP+ LHCL + L E + + D L D + +H+ + SDNV+AA
Sbjct: 203 SLIAAKSIPKGLHCLAMRLMEERIAHPE-KYTDEGKDRPRELEDPNLYHYAIFSDNVIAA 261
Query: 115 SVVAKSLVQNSLQPWKVVLHIITDKKTYNSMQAWFSLHSLSPAIIEVKSLQDFDWFREGK 174
SVV S V+N+ +PWK V H++TDK +MQ F L A +EVK+++D+ +
Sbjct: 262 SVVVNSAVKNAKEPWKHVFHVVTDKMNLGAMQVMFKLKEYKGAHVEVKAVEDYTFLNSSY 321
Query: 175 VSLLEAMEKDQHVRSRFRGGSSAIISNTAEKPEDIAAKLKALSPTYSSVMNHIRIYIPEL 234
V +L+ +E + F N E +K +P Y S++NH+R Y+PE+
Sbjct: 322 VPVLKQLESANLQKFYFE--------NKLENATKDTTNMKFRNPKYLSILNHLRFYLPEM 373
Query: 235 FPSLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCKGKDKRVMSKMLKSYLNFSHP 294
+P L +++FLD+D+VVQ DL+ LW+IDM+GKVNGAVETC G R Y+NFSHP
Sbjct: 374 YPKLHRILFLDDDVVVQKDLTGLWEIDMDGKVNGAVETCFGSFHRYAQ-----YMNFSHP 428
Query: 295 LISKNFDPNECAWAYGMNILDLEAWRKTNISYTYHYWVEQNIKSDLSLWHLGTLPPGLIA 354
LI + F+P CAWAYGMN DL+AWR+ + YHYW QN+ + +LW LGTLPPGLI
Sbjct: 429 LIKEKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYW--QNLNENRALWKLGTLPPGLIT 486
Query: 355 FHGNVHTIDPFWHMLGLGYQENTNLVDAKNAGVVHFNGWAKPWLDIAFPQLKPLWTKYVD 414
F+ +D WH+LGLGY + ++ + +NA VVHFNG KPWLDIA Q +PLWTK+VD
Sbjct: 487 FYSTTKPLDKSWHVLGLGYNPSISMDEIRNAAVVHFNGNMKPWLDIAMNQFRPLWTKHVD 546
Query: 415 FSDNFIESCH 424
+ F+++C+
Sbjct: 547 YDLEFVQACN 556
>GSPA_BACSU (P25148) General stress protein A
Length = 286
Score = 53.1 bits (126), Expect = 1e-06
Identities = 47/195 (24%), Positives = 88/195 (45%), Gaps = 30/195 (15%)
Query: 215 ALSPTYSSVMNHIRIYIPELFP--SLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVET 272
A+ ++ + + RI IP+L S+ +++++D D +V D+S LWD+D+ AVE
Sbjct: 79 AVESSHITKAAYYRISIPDLIKDESIKRMIYIDCDALVLEDISKLWDLDIAPYTVAAVED 138
Query: 273 CKGKDKRVMSKMLKSYLNFSHPLISKNFDPNECAWAYGMNILDLEAWRKTNISYTYHYWV 332
G+ +R+ + K F+ G+ I+D E+WRK NI+ ++
Sbjct: 139 A-GQHERLKEMNVTD--------TGKYFNS-------GIMIIDFESWRKQNITEKVINFI 182
Query: 333 EQNIKSDLSLWHLGTLPPGLIAFHGNVHTIDPFWH---MLGLGYQENTNLVDAK------ 383
++ D + H ++ + + + P W+ + L + + L+ K
Sbjct: 183 NEHPDEDFLVLHDQDALNAIL--YDQWYELHPRWNAQTYIMLKLKTPSTLLGRKQYNETR 240
Query: 384 -NAGVVHFNGWAKPW 397
N +VHF G KPW
Sbjct: 241 ENPAIVHFCGGEKPW 255
>UGGG_SCHPO (Q09140) UDP-glucose:glycoprotein glucosyltransferase
precursor (EC 2.4.1.-) (UDP--Glc:glycoprotein
glucosyltransferase) (UGT)
Length = 1448
Score = 38.1 bits (87), Expect = 0.044
Identities = 17/51 (33%), Positives = 31/51 (60%), Gaps = 1/51 (1%)
Query: 229 IYIPELFP-SLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCKGKDK 278
+++ LFP L KV+++D D +V+ DL L D+D++G G C +++
Sbjct: 1242 LFLDVLFPLELHKVIYVDADQIVRADLQELMDMDLHGAPYGYTPMCDSREE 1292
>UGG2_HUMAN (Q9NYU1) UDP-glucose:glycoprotein glucosyltransferase 2
precursor (EC 2.4.1.-) (UDP--Glc:glycoprotein
glucosyltransferase 2) (UGT 2) (HUGT2)
Length = 1516
Score = 37.4 bits (85), Expect = 0.074
Identities = 17/49 (34%), Positives = 30/49 (60%), Gaps = 1/49 (2%)
Query: 229 IYIPELFP-SLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCKGK 276
+++ LFP ++DK++F+D D +V+ DL L D D++G G C +
Sbjct: 1316 LFLDVLFPLAVDKIIFVDADQIVRHDLKELRDFDLDGAPYGYTPFCDSR 1364
>UGGG_DROME (Q09332) UDP-glucose:glycoprotein glucosyltransferase
precursor (EC 2.4.1.-) (UDP--Glc:glycoprotein
glucosyltransferase) (dUGT)
Length = 1548
Score = 37.0 bits (84), Expect = 0.097
Identities = 23/94 (24%), Positives = 49/94 (51%), Gaps = 8/94 (8%)
Query: 229 IYIPELFP-SLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCKGKDKRVMSKMLKS 287
+++ LFP ++ K++F+D D +V+TD+ L+D+D+ G C + + + K
Sbjct: 1325 LFLDVLFPLNVRKIIFVDADAIVRTDIKELYDMDLGGAPYAYTPFCDSRKEMEGFRFWKQ 1384
Query: 288 YLNFSHPLISKNFDPNECAWAYGMNILDLEAWRK 321
SH L+ + + + + ++DL+ +RK
Sbjct: 1385 GYWRSH-LMGRRYHIS------ALYVVDLKRFRK 1411
>RFAJ_ECOLI (P27129) Lipopolysaccharide 1,2-glucosyltransferase (EC
2.4.1.58)
Length = 338
Score = 36.2 bits (82), Expect = 0.17
Identities = 39/178 (21%), Positives = 80/178 (44%), Gaps = 25/178 (14%)
Query: 237 SLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCKGKDKRVMSKMLKSYLNFSHP-L 295
+LD++++LD D+V + D+S L + +NG V V+ + ++ +S++ S P L
Sbjct: 122 TLDRLLYLDADVVCKGDISQLLHLGLNGAVAAVVKDVEPMQEKAVSRL-------SDPEL 174
Query: 296 ISKNFDPNECAWAYGMNILDLEAWRKTNIS--YTYHYWVEQNIKSDLSLWHLGTLPPGLI 353
+ + F+ G+ LDL+ W ++ + N+ + L G+
Sbjct: 175 LGQYFNS-------GVVYLDLKKWADAKLTEKALSILMSKDNVYKYPDQDVMNVLLKGMT 227
Query: 354 AF----HGNVHTIDPFWHMLGLGYQENTNLVDAKNAGVVHFNGWAKPWLDIA-FPQLK 406
F + ++TI + +Q L+ ++ ++H+ G KPW A +P +K
Sbjct: 228 LFLPREYNTIYTIKS--ELKDKTHQNYKKLI-TESTLLIHYTGATKPWHKWAIYPSVK 282
>Y258_HAEIN (P43974) Putative glycosyl transferase HI0258 (EC
2.-.-.-)
Length = 330
Score = 34.7 bits (78), Expect = 0.48
Identities = 22/118 (18%), Positives = 52/118 (43%), Gaps = 19/118 (16%)
Query: 220 YSSVMNHIRIYIPELFPSLDKVVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCKGKDKR 279
Y S+ + R+ + + +++K +++D D + + L LW+ID+ N + C+
Sbjct: 115 YISLATYARLNLTKYIKNIEKAIYIDVDTLTNSSLQELWNIDI---TNYYLAACRDTFID 171
Query: 280 VMSKMLKSYLNFSHPLISKNFDPNECAWAY---GMNILDLEAWRKTNISYTYHYWVEQ 334
V ++ K + ++Y G+ +++L W++ NI W+ +
Sbjct: 172 VKNEAYKKTIGLE-------------GYSYFNAGILLINLNKWKEENIFQKSINWMNK 216
>VMT8_MYXVL (P22611) M-T8 protein
Length = 515
Score = 33.5 bits (75), Expect = 1.1
Identities = 26/101 (25%), Positives = 44/101 (42%), Gaps = 12/101 (11%)
Query: 256 PLWDIDMNGKVNGAVETCKGKDKRVMSKMLKSYLNFSHPLISKNFDPNECAWAYGMNILD 315
PL+ + + GK+ +GK R +L +Y + + L + NF ++++D
Sbjct: 5 PLYKLFLKGKLCDVEIVAEGKSIRAHRLVLSAYSKYFYNLFNGNF------LEKNVDVID 58
Query: 316 LEAWRKTNISYTYHYWVEQ------NIKSDLSLWHLGTLPP 350
LEA KT Y+ + E N +S SL H + P
Sbjct: 59 LEADYKTVFDVIYYMYTESIELHKGNTESIFSLVHYLQIKP 99
>LAGE_MOUSE (Q9Z1M7) Glycosyltransferase-like protein LARGE (EC
2.4.-.-) (Acetylglucosaminyltransferase-like protein)
Length = 756
Score = 32.3 bits (72), Expect = 2.4
Identities = 13/42 (30%), Positives = 26/42 (60%), Gaps = 1/42 (2%)
Query: 220 YSSVMNHIRIYIPELFPS-LDKVVFLDNDIVVQTDLSPLWDI 260
YS + +++ + + P+ L++V+ LD DI TD++ LW +
Sbjct: 216 YSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAV 257
>LAGE_HUMAN (O95461) Glycosyltransferase-like protein LARGE (EC
2.4.-.-) (Acetylglucosaminyltransferase-like protein)
Length = 756
Score = 32.3 bits (72), Expect = 2.4
Identities = 13/42 (30%), Positives = 26/42 (60%), Gaps = 1/42 (2%)
Query: 220 YSSVMNHIRIYIPELFPS-LDKVVFLDNDIVVQTDLSPLWDI 260
YS + +++ + + P+ L++V+ LD DI TD++ LW +
Sbjct: 216 YSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAV 257
>PURA_DROME (Q9Y0Y2) Adenylosuccinate synthetase (EC 6.3.4.4)
(IMP--aspartate ligase) (AdSS) (AMPSase)
Length = 447
Score = 30.8 bits (68), Expect = 6.9
Identities = 26/115 (22%), Positives = 52/115 (44%), Gaps = 10/115 (8%)
Query: 169 WFREGKVSLLEAMEKDQHVRSRFRGGSSA---IISNTAEKPEDIAAKLKALSPTYSSVMN 225
W EGK +++ + D + R +GG++A +++N E + S + N
Sbjct: 34 WGDEGKGKVVDMLASDVDIVCRCQGGNNAGHTVVANGTEFDFHLLPSGVVNEKCVSVIGN 93
Query: 226 HIRIYIPELFPSLDK-----VVFLDNDIVVQTDLSPLWDIDMNGKVNGAVETCKG 275
+ I++P LF + K + L+N +++ ++D + V+G E KG
Sbjct: 94 GVVIHLPSLFDEVLKNEAKGLQHLENRLIISDRAHLVFDFHQH--VDGMQEAEKG 146
>PEX6_PICPA (P33289) Peroxisomal biogenesis factor 6 (Peroxin-6)
(Peroxisome biosynthesis protein PAS5)
Length = 1165
Score = 30.8 bits (68), Expect = 6.9
Identities = 26/93 (27%), Positives = 42/93 (44%), Gaps = 6/93 (6%)
Query: 104 FVLASDNVLAASVVAKSLVQNSLQPWKVVLHIITDKKTYNSMQAWFSLHSLSPAIIEVKS 163
+ L SD +L A + V ++ + L +K+ S + WF + I + +
Sbjct: 1033 YALCSDAMLNAMTRIANTVDEKIKRYNEEL----PEKSQVSTRWWFDNVATKEDIDVLVT 1088
Query: 164 LQDFDWFREGKVSLLEAMEKDQH--VRSRFRGG 194
L+DFD R+ V + A E D + VR F GG
Sbjct: 1089 LEDFDKSRKELVPSVSAEELDHYLRVRQNFEGG 1121
>MFD_RICPR (O05955) Transcription-repair coupling factor (TRCF)
Length = 1120
Score = 30.4 bits (67), Expect = 9.1
Identities = 18/72 (25%), Positives = 35/72 (48%)
Query: 183 KDQHVRSRFRGGSSAIISNTAEKPEDIAAKLKALSPTYSSVMNHIRIYIPELFPSLDKVV 242
+D +R FRGG S ++ + EDI +LK + P S + H ++ ++ + +
Sbjct: 779 RDALLREHFRGGRSFYVAPRIKDMEDIEKQLKQIVPELSYKIAHGKMTPSKIDEVMSEFY 838
Query: 243 FLDNDIVVQTDL 254
DI++ T +
Sbjct: 839 VGKFDILISTTI 850
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.137 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,611,776
Number of Sequences: 164201
Number of extensions: 2194810
Number of successful extensions: 5074
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 5051
Number of HSP's gapped (non-prelim): 18
length of query: 427
length of database: 59,974,054
effective HSP length: 113
effective length of query: 314
effective length of database: 41,419,341
effective search space: 13005673074
effective search space used: 13005673074
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 67 (30.4 bits)
Medicago: description of AC148653.4