BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC148651.4 + phase: 0 /pseudo
         (764 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YCBX_ECOLI (P75863) Hypothetical protein ycbX                          82  7e-15
NFS1_YEAST (P25374) Cysteine desulfurase, mitochondrial precurso...    65  5e-10
NFS1_ASHGO (O60028) Cysteine desulfurase, mitochondrial precurso...    62  4e-09
CSD_UREPA (Q9PQ36) Probable cysteine desulfurase (EC 2.8.1.7)          62  6e-09
ISCS_ECOLI (P39171) Cysteine desulfurase (EC 2.8.1.7) (ThiI tran...    60  2e-08
ISCS_SALTY (Q8ZN40) Cysteine desulfurase (EC 2.8.1.7) (ThiI tran...    59  6e-08
ISCS_SALTI (Q8Z4N0) Cysteine desulfurase (EC 2.8.1.7) (ThiI tran...    59  6e-08
NIFS_FRASE (Q9Z5X5) Cysteine desulfurase (EC 2.8.1.7) (Nitrogena...    57  1e-07
CSD_BORBU (O51111) Probable cysteine desulfurase (EC 2.8.1.7)          56  3e-07
NFS1_HUMAN (Q9Y697) Cysteine desulfurase, mitochondrial precurso...    56  4e-07
NFS1_RAT (Q99P39) Cysteine desulfurase, mitochondrial precursor ...    55  9e-07
CSD_AERPE (Q9YAB6) Probable cysteine desulfurase (EC 2.8.1.7)          55  9e-07
NFS1_MOUSE (Q9Z1J3) Cysteine desulfurase, mitochondrial precurso...    54  2e-06
ISCS_BUCBP (Q89A19) Cysteine desulfurase (EC 2.8.1.7)                  53  3e-06
CSD_PYRAB (Q9V242) Probable cysteine desulfurase (EC 2.8.1.7)          52  6e-06
CSD_STAAN (P99177) Probable cysteine desulfurase (EC 2.8.1.7)          50  2e-05
CSD_STAAM (P63518) Probable cysteine desulfurase (EC 2.8.1.7)          50  2e-05
ISC1_ARCFU (O30052) Probable cysteine desulfurase 1 (EC 2.8.1.7)       49  6e-05
CSD_STAEP (Q8CTA4) Probable cysteine desulfurase (EC 2.8.1.7)          47  2e-04
CSD_MYCPN (P75298) Probable cysteine desulfurase (EC 2.8.1.7)          46  3e-04

>YCBX_ECOLI (P75863) Hypothetical protein ycbX
          Length = 369

 Score = 81.6 bits (200), Expect = 7e-15
 Identities = 74/257 (28%), Positives = 114/257 (43%), Gaps = 43/257 (16%)

Query: 510 NPVEASVQAVGL---LATMGSLKHDREWILKSLSGEILTLKRVPEMGLISSFIDLSQGML 566
           +PV+ S++ +GL   LA +  L  DR +++    G  +T ++ P+M            + 
Sbjct: 10  HPVK-SMRGIGLTHALADVSGLAFDRIFMITEPDGTFITARQFPQM------------VR 56

Query: 567 FVESP-----HCKERLQIRLQLDFYDSAIQDI--ELQGQRYKVYSYDNETNSWFSKAIER 619
           F  SP     H          + F D A QD   E+ G  +      +  N W S    R
Sbjct: 57  FTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIAPDAINKWLSGFFSR 116

Query: 620 PCTLLRYSGSSHDFVLDRTKDIVTCKDTNSAVSFANEGQFLLVSEESVSDLNKRLCSDVQ 679
              L R+ G      + R   +         +SFA+   +LL +E S+ DL +R  + V+
Sbjct: 117 EVQL-RWVGPQMTRRVKRHNTV--------PLSFADGYPYLLANEASLRDLQQRCPASVK 167

Query: 680 MDMCETEIEINTNRFRPNLVVSGGRPYDEDGWSDIRIGNKYFRSLGGCNRCQVINLTLNA 739
           M+           +FRPNLVVSG   ++ED W  IRIG+  F  +  C+RC    ++   
Sbjct: 168 ME-----------QFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEK 216

Query: 740 GQVQKSKEPLATLASYR 756
           GQ   + EPL TL S+R
Sbjct: 217 GQKHPAGEPLKTLQSFR 233


>NFS1_YEAST (P25374) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7) (tRNA splicing protein SPL1)
          Length = 497

 Score = 65.5 bits (158), Expect = 5e-10
 Identities = 65/257 (25%), Positives = 113/257 (43%), Gaps = 37/257 (14%)

Query: 38  VYLDHAGATLYSELQMESVFKDLTTNVYGNPHSQSDSSA-ATHDIVRDARQQVLDYCNAS 96
           +YLD    T      ++++ K   T +YGNPHS + S    T+  V +AR  V    NA 
Sbjct: 100 IYLDMQATTPTDPRVLDTMLK-FYTGLYGNPHSNTHSYGWETNTAVENARAHVAKMINAD 158

Query: 97  PEDYKCIFTSGATAALKLVGEAFP---WSCNSNFMYTMENHNSVLGIREYALGQGAAAIA 153
           P++   IFTSGAT +  +V +  P        + + T   H  VL      + +G     
Sbjct: 159 PKE--IIFTSGATESNNMVLKGVPRFYKKTKKHIITTRTEHKCVLEAARAMMKEGFEVTF 216

Query: 154 VDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEE----PMECNFSGLRFDLDLAKIIK 209
           ++++D                     + +  L+E E    P  C  S +  + ++  I  
Sbjct: 217 LNVDD---------------------QGLIDLKELEDAIRPDTCLVSVMAVNNEIGVI-- 253

Query: 210 EDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGLGALV 269
               K +GA +C+K +     DAA+    +  D+++  +D +++S +K++G P G+GA+ 
Sbjct: 254 -QPIKEIGA-ICRKNKIYFHTDAAQAYGKIHIDVNEMNIDLLSISSHKIYG-PKGIGAIY 310

Query: 270 VRNDAAKLLKKSYFSGG 286
           VR      L+     GG
Sbjct: 311 VRRRPRVRLEPLLSGGG 327


>NFS1_ASHGO (O60028) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7) (tRNA splicing protein SPL1)
          Length = 490

 Score = 62.4 bits (150), Expect = 4e-09
 Identities = 76/297 (25%), Positives = 131/297 (43%), Gaps = 50/297 (16%)

Query: 38  VYLDHAGATLYSELQMESVFKDLTTNVYGNPHSQSDSSA-ATHDIVRDARQQVLDYCNAS 96
           +YLD    T      ++++ K   T +YGNPHS + S    T   V  AR+ V D   A 
Sbjct: 93  IYLDMQATTPTDPRVVDTMLK-FYTGLYGNPHSNTHSYGWETSQEVEKARKNVADVIKAD 151

Query: 97  PEDYKCIFTSGATAA--LKLVGEA-FPWSCNSNFMYTMENHNSVLGIREYALGQGAAAIA 153
           P++   IFTSGAT +  + L G A F     ++ + T   H  VL        +G     
Sbjct: 152 PKE--IIFTSGATESNNMALKGVARFYKKRKNHIITTRTEHKCVLEAARSMKDEG----- 204

Query: 154 VDIEDVHPRIEGEKFPTKISLHQEQR--RKVTGLQEEEPMECNFSGLRFDLDLAKIIKED 211
            D+  ++   +G      +SL + ++  R  T L        +   +  ++ + + IKE 
Sbjct: 205 FDVTFLNVNEDG-----LVSLEELEQAIRPETSL-------VSVMSVNNEIGVVQPIKE- 251

Query: 212 SSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGLGALVVR 271
               +GA +C++ +     DAA+    +P D+ +  +D +++S +K++G P G+GAL VR
Sbjct: 252 ----IGA-ICRRHKVFFHSDAAQAYGKIPIDVDEMNIDLLSISSHKIYG-PKGIGALYVR 305

Query: 272 NDAAKLLKKSYFSGGTVAASIADIDFIKRREGIEELFEDGTVSFLSIASIRHGFKIL 328
               ++  +   SGG                G E  F  GT+    +  + H  K++
Sbjct: 306 R-RPRVRMEPLLSGG----------------GQERGFRSGTLPPPLVVGLGHAAKLM 345


>CSD_UREPA (Q9PQ36) Probable cysteine desulfurase (EC 2.8.1.7)
          Length = 402

 Score = 62.0 bits (149), Expect = 6e-09
 Identities = 64/260 (24%), Positives = 113/260 (42%), Gaps = 28/260 (10%)

Query: 31  FNRLQDLVYLDHAGATLYSELQMESVFKDLTTNVYGNPH-SQSDSSAATHDIVRDARQQV 89
           F   +D++YLD +  +L  ++ ++++  D  T    NPH + S+ +  TH+I+ + R  V
Sbjct: 11  FKNNKDVIYLDSSATSLKPQVVIDAIV-DYYTKYSTNPHNTDSNFTFHTHEIMNETRANV 69

Query: 90  LDYCNASPEDYKCIFTSGATAALKLVGEAFPWSCNSN---FMYTMENHNSVLGIREYALG 146
             + NA+ ++   IFTSGAT +L L+               +  +E+ +++L   +    
Sbjct: 70  AKFINANFDE--IIFTSGATESLNLIANGLRPHLKKGDEIILTYIEHASNLLPWYKLRDD 127

Query: 147 QGAAAIAVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAK 206
            G   I  + ++  P++    F   IS     + KV        +  N       +   K
Sbjct: 128 LGVKIIFANQKNQFPQL--SDFLKVIS----PKTKVVSFASGGNLIGNVLDENLIIKNIK 181

Query: 207 IIKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGLG 266
            IK D               LV +DA +       D+ K   DF+  S +KL G PTG+G
Sbjct: 182 KIKPDV--------------LVCVDATQSIQHRMFDVKKCKSDFMVFSAHKLLG-PTGIG 226

Query: 267 ALVVRNDAAKLLKKSYFSGG 286
              ++ND  K ++   + GG
Sbjct: 227 VAYIKNDLIKKMQPLKYGGG 246


>ISCS_ECOLI (P39171) Cysteine desulfurase (EC 2.8.1.7) (ThiI
           transpersulfidase) (NifS protein homolog)
          Length = 404

 Score = 60.1 bits (144), Expect = 2e-08
 Identities = 67/262 (25%), Positives = 113/262 (42%), Gaps = 45/262 (17%)

Query: 38  VYLDHAGATLYSELQMESVFKDLTTN-VYGNPHSQSDSSA-ATHDIVRDARQQVLDYCNA 95
           +YLD++  T       E + + +T +  +GNP S+S        + V  AR Q+ D   A
Sbjct: 5   IYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFGWQAEEAVDIARNQIADLVGA 64

Query: 96  SPEDYKCIFTSGATAA--LKLVGEA-FPWSCNSNFMYTMENHNSVLGIREYALGQGAAAI 152
            P +   +FTSGAT +  L + G A F      + + +   H +VL              
Sbjct: 65  DPREI--VFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVL-------------- 108

Query: 153 AVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKI----- 207
                D   ++E E F  +++    QR  +  L+E E      + +R D  L  I     
Sbjct: 109 -----DTCRQLEREGF--EVTYLAPQRNGIIDLKELE------AAMRDDTILVSIMHVNN 155

Query: 208 ---IKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTG 264
              + +D + I    +C+    +  +DA +    +P DLS+  VD ++ S +K++G P G
Sbjct: 156 EIGVVQDIAAI--GEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYG-PKG 212

Query: 265 LGALVVRNDAAKLLKKSYFSGG 286
           +GAL VR      ++     GG
Sbjct: 213 IGALYVRRKPRVRIEAQMHGGG 234


>ISCS_SALTY (Q8ZN40) Cysteine desulfurase (EC 2.8.1.7) (ThiI
           transpersulfidase) (NifS protein homolog)
          Length = 404

 Score = 58.5 bits (140), Expect = 6e-08
 Identities = 67/262 (25%), Positives = 112/262 (42%), Gaps = 45/262 (17%)

Query: 38  VYLDHAGATLYSELQMESVFKDLTTN-VYGNPHSQSDSSA-ATHDIVRDARQQVLDYCNA 95
           +YLD++  T       E + + LT +  +GNP S+S        + V  AR Q+ +   A
Sbjct: 5   IYLDYSATTPVDPRVAEKMMQFLTLDGTFGNPASRSHRFGWQAEEAVDIARNQIAELVGA 64

Query: 96  SPEDYKCIFTSGATAA--LKLVGEA-FPWSCNSNFMYTMENHNSVLGIREYALGQGAAAI 152
            P +   +FTSGAT +  L + G A F      + + +   H +VL              
Sbjct: 65  DPREI--VFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVL-------------- 108

Query: 153 AVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKI----- 207
                D   ++E E F  +++    QR  +  L E E      + +R D  L  I     
Sbjct: 109 -----DTCRQLEREGF--EVTYLAPQRNGIIDLNELE------AAMRDDTILVSIMHVNN 155

Query: 208 ---IKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTG 264
              + +D + I    +C+    +  +DA +    +P DLS+  VD ++ S +K++G P G
Sbjct: 156 EIGVVQDIATI--GEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYG-PKG 212

Query: 265 LGALVVRNDAAKLLKKSYFSGG 286
           +GAL VR      ++     GG
Sbjct: 213 IGALYVRRKPRIRIEAQMHGGG 234


>ISCS_SALTI (Q8Z4N0) Cysteine desulfurase (EC 2.8.1.7) (ThiI
           transpersulfidase) (NifS protein homolog)
          Length = 404

 Score = 58.5 bits (140), Expect = 6e-08
 Identities = 67/262 (25%), Positives = 112/262 (42%), Gaps = 45/262 (17%)

Query: 38  VYLDHAGATLYSELQMESVFKDLTTN-VYGNPHSQSDSSA-ATHDIVRDARQQVLDYCNA 95
           +YLD++  T       E + + LT +  +GNP S+S        + V  AR Q+ +   A
Sbjct: 5   IYLDYSATTPVDPRVAEKMMQFLTLDGTFGNPASRSHRFGWQAEEAVDIARNQIAELVGA 64

Query: 96  SPEDYKCIFTSGATAA--LKLVGEA-FPWSCNSNFMYTMENHNSVLGIREYALGQGAAAI 152
            P +   +FTSGAT +  L + G A F      + + +   H +VL              
Sbjct: 65  DPREI--VFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVL-------------- 108

Query: 153 AVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKI----- 207
                D   ++E E F  +++    QR  +  L E E      + +R D  L  I     
Sbjct: 109 -----DTCRQLEREGF--EVTYLAPQRNGIIDLNELE------AAMRDDTILVSIMHVNN 155

Query: 208 ---IKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTG 264
              + +D + I    +C+    +  +DA +    +P DLS+  VD ++ S +K++G P G
Sbjct: 156 EIGVVQDIATI--GEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYG-PKG 212

Query: 265 LGALVVRNDAAKLLKKSYFSGG 286
           +GAL VR      ++     GG
Sbjct: 213 IGALYVRRKPRIRIEAQMHGGG 234


>NIFS_FRASE (Q9Z5X5) Cysteine desulfurase (EC 2.8.1.7) (Nitrogenase
           metalloclusters biosynthesis protein nifS)
          Length = 419

 Score = 57.4 bits (137), Expect = 1e-07
 Identities = 65/263 (24%), Positives = 101/263 (37%), Gaps = 40/263 (15%)

Query: 37  LVYLDHAGATLYSELQMESVFKDLTTNVYGNPHSQSDSSAATHDIVRDARQQVLDYCNAS 96
           ++YLDH  AT   + ++  V   L T  + NP S+         IV  AR++V     A 
Sbjct: 1   MIYLDH-NATTPVDPRVVEVMLPLFTERFANPASKHAPGQEATRIVERARREVASLAGAR 59

Query: 97  PEDYKCIFTSGATAALKL-----VGEAFPWSCNSNFMYTMENHNSVLGIREYALGQGAAA 151
           P +   +FTSGAT A  L     +  A P       +     H +VLG  + A+  G+A 
Sbjct: 60  PREI--VFTSGATEAANLAIGGVLATAEPG--RRRILVGATEHPAVLGAADAAVSAGSAV 115

Query: 152 IAVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKIIKED 211
            A          +G     +I++H +    V  L+            R   D+A +    
Sbjct: 116 SA----------DGTTAAERIAVHPDGTVDVDDLRR-----------RLGPDVALVAVMA 154

Query: 212 SSKILGA--------SVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPT 263
           ++   GA           +        D  +    +P +  +  VD    S +KL+G P 
Sbjct: 155 ANNETGAVNDPRPLVEAARSAGRCCCADVTQALGRVPVEFERTGVDLAVSSAHKLYG-PK 213

Query: 264 GLGALVVRNDAAKLLKKSYFSGG 286
           G+GAL+   DA   L      GG
Sbjct: 214 GVGALIASKDAWSRLAPRVHGGG 236


>CSD_BORBU (O51111) Probable cysteine desulfurase (EC 2.8.1.7)
          Length = 422

 Score = 56.2 bits (134), Expect = 3e-07
 Identities = 70/311 (22%), Positives = 131/311 (41%), Gaps = 39/311 (12%)

Query: 37  LVYLDHAGAT------LYSELQMESVFKDLTTNVYGNPHSQSDSSAATHDIVRDARQQVL 90
           ++Y D+A  +      +YS ++    +++   NV+ + H  +  S+     +   R+ V 
Sbjct: 32  IIYFDNAATSQKPKNVIYSNVEY---YENYNANVHRSGHKFAIQSSIK---IEKTRELVK 85

Query: 91  DYCNASPEDYKCIFTSGATAALKLVGEAFPWSCNSNFMYTMENHNSVLGIREYALGQGAA 150
           ++ NA       IFTSG T  +  +  +F +S      Y  +    +L   E+       
Sbjct: 86  NFINAESAK-NIIFTSGTTDGINTIASSFFYS-----KYFKKKDEIILTTLEHNSNLLPW 139

Query: 151 AIAVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDL----DLAK 206
               ++ ++  +I+  KF     +  E+  K   L  E+    + SG+   L    DL  
Sbjct: 140 VNLANLANL--KIKLAKFNEMGIITPEEIEK---LITEKTKLISISGINNTLGTINDLES 194

Query: 207 IIKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGLG 266
           I K          + KK    + +DAA+ +  +  D+ K   DF+  S +K+   PTG+G
Sbjct: 195 IGK----------IAKKYNICLFVDAAQMAPHIKIDVKKIGCDFLVFSGHKMLA-PTGIG 243

Query: 267 ALVVRNDAAKLLKKSYFSGGTVAASIADIDFIK-RREGIEELFEDGTVSFLSIASIRHGF 325
            L + N+ A+ L  S   G TV     + + IK +       FE GT +   I  +    
Sbjct: 244 ILYISNNMAEKLHSSKLGGNTVEEIFIENEKIKFKASDSPNKFESGTPNIAGIIGLEEAI 303

Query: 326 KILNSLTVSAI 336
           K ++++++  I
Sbjct: 304 KYIDNISMDFI 314


>NFS1_HUMAN (Q9Y697) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7) (HUSSY-08)
          Length = 457

 Score = 55.8 bits (133), Expect = 4e-07
 Identities = 65/267 (24%), Positives = 115/267 (42%), Gaps = 45/267 (16%)

Query: 17  PNAARTIDQIRATEFNRLQDLVYLDHAGATLYSELQMESVFKDLTTNVYGNPHSQSDSSA 76
           P +A   D   A E   +   +Y+D    T      ++++   L  N YGNPHS++ +  
Sbjct: 38  PQSAVPADTTAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLI-NYYGNPHSRTHAYG 96

Query: 77  ATHDIVRD-ARQQVLDYCNASPEDYKCIFTSGATAA--LKLVGEA-FPWSCNSNFMYTME 132
              +   + ARQQV     A P +   IFTSGAT +  + + G A F  S   + + T  
Sbjct: 97  WESEAAMERARQQVASLIGADPREI--IFTSGATESNNIAIKGVARFYRSRKKHLITTQT 154

Query: 133 NHNSVLGIREYALGQGAAAIAVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPME 192
            H  VL                   D    +E E F  +++    Q+  +  L+E E   
Sbjct: 155 EHKCVL-------------------DSCRSLEAEGF--QVTYLPVQKSGIIDLKELE--- 190

Query: 193 CNFSGLRFDLDLAKI--------IKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLS 244
              + ++ D  L  +        +K+  ++I    +C   +     DAA+    +P D++
Sbjct: 191 ---AAIQPDTSLVSVMTVNNEIGVKQPIAEI--GRICSSRKVYFHTDAAQAVGKIPLDVN 245

Query: 245 KYPVDFVALSFYKLFGYPTGLGALVVR 271
              +D +++S +K++G P G+GA+ +R
Sbjct: 246 DMKIDLMSISGHKIYG-PKGVGAIYIR 271


>NFS1_RAT (Q99P39) Cysteine desulfurase, mitochondrial precursor (EC
           2.8.1.7)
          Length = 451

 Score = 54.7 bits (130), Expect = 9e-07
 Identities = 61/246 (24%), Positives = 108/246 (43%), Gaps = 45/246 (18%)

Query: 38  VYLDHAGATLYSELQMESVFKDLTTNVYGNPHSQSDSSAATHDIVRD-ARQQVLDYCNAS 96
           +Y+D    T      ++++   L  N YGNPHS++ +     +   + ARQQV     A 
Sbjct: 53  LYMDVRATTPLDPRVLDAMLPYLV-NYYGNPHSRTHAYGWESEAAMERARQQVASLIGAD 111

Query: 97  PEDYKCIFTSGATAA--LKLVGEA-FPWSCNSNFMYTMENHNSVLGIREYALGQGAAAIA 153
           P +   IFTSGAT +  + + G A F  S   + + T   H  VL               
Sbjct: 112 PREI--IFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVL--------------- 154

Query: 154 VDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKI------ 207
               D    +E E F  +++    Q+  +  L+E E      + ++ D  L  +      
Sbjct: 155 ----DSCRSLEAEGF--RVTYLPVQKSGIIDLKELE------AAIQPDTSLVSVMTVNNE 202

Query: 208 --IKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGL 265
             +K+  ++I    +C   +     DAA+    +P D++   +D +++S +KL+G P G+
Sbjct: 203 IGVKQPIAEI--GQICSSRKLYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYG-PKGV 259

Query: 266 GALVVR 271
           GA+ +R
Sbjct: 260 GAIYIR 265


>CSD_AERPE (Q9YAB6) Probable cysteine desulfurase (EC 2.8.1.7)
          Length = 411

 Score = 54.7 bits (130), Expect = 9e-07
 Identities = 83/348 (23%), Positives = 143/348 (40%), Gaps = 64/348 (18%)

Query: 29  TEFNRLQ-DLVYLDHAGATLYSELQMESVFKDLTTNVYGNPHSQSDS-SAATHDIVRDAR 86
           +EF  L+  +VYLD+A +TL     +E++ ++ +   Y N H      S        DA 
Sbjct: 11  SEFPELERGIVYLDNAASTLKPRRVVEAM-REFSYRSYANVHRGVHRLSMEASKAYEDAH 69

Query: 87  QQVLDYCNASPEDYKCIFTSGATAALKLVGEAFPWSC---NSNFMYTMENHNSVL--GIR 141
           + V      S ++   +FT   T A++L      ++        + T  +H+S L   +R
Sbjct: 70  EVVARLVGGSWDEV--VFTRNTTEAMQLAALTLAYNGLLRGGEVLVTAADHHSTLLPWVR 127

Query: 142 EYALGQGAAAIAVDIEDVHPRIEGEKFPTKISLH----QEQRRKVTGLQEEEPMECNFSG 197
              LG G A I          ++G   P    L     ++ R    G         N +G
Sbjct: 128 AARLGGGRARILP--------LDGRGVPRWDLLEDYITEDTRAVAVGHVS------NVTG 173

Query: 198 LRFDLDLAKIIKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYK 257
           +   ++   I+K  ++K +GA        LV++D+A+G   +P D  +  VD  A S +K
Sbjct: 174 VVAPVE--DIVK--AAKKVGA--------LVVLDSAQGVPHLPVDFRRMGVDMAAFSGHK 221

Query: 258 LFGYPTGLGALVVRNDAAKLLKKSYFSGGTVAASIADIDFIKRREGIEEL--------FE 309
           + G PTG+G L  R D  + L+     GGTV+        ++ + G  E+        FE
Sbjct: 222 MLG-PTGIGVLWARRDLLEELEPPLGGGGTVSR-------VRLQGGSVEIEWEEPPWKFE 273

Query: 310 DGTVSFLSIASIRHGFKILNSLTVSAISR--------TYNISCPVYEE 349
            GT   +    +     +L  + +  ++R        T  +  P+Y E
Sbjct: 274 AGTPPIIEAVGLAEAANMLMEIGMEHVARHEDELTSHTMKLLEPLYSE 321


>NFS1_MOUSE (Q9Z1J3) Cysteine desulfurase, mitochondrial precursor
           (EC 2.8.1.7) (m-Nfs1)
          Length = 451

 Score = 53.9 bits (128), Expect = 2e-06
 Identities = 61/246 (24%), Positives = 108/246 (43%), Gaps = 45/246 (18%)

Query: 38  VYLDHAGATLYSELQMESVFKDLTTNVYGNPHSQSDSSAATHDIVRD-ARQQVLDYCNAS 96
           +Y+D    T      ++++   L  N YGNPHS++ +     +   + ARQQV     A 
Sbjct: 53  LYMDVQATTPLDPRVLDAMLPYLV-NYYGNPHSRTHAYGWESEAAMERARQQVASLIGAD 111

Query: 97  PEDYKCIFTSGATAA--LKLVGEA-FPWSCNSNFMYTMENHNSVLGIREYALGQGAAAIA 153
           P +   IFTSGAT +  + + G A F  S   + + T   H  VL               
Sbjct: 112 PREI--IFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVL--------------- 154

Query: 154 VDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKI------ 207
               D    +E E F  +++    Q+  +  L+E E      + ++ D  L  +      
Sbjct: 155 ----DSCRSLEAEGF--RVTYLPVQKSGIIDLKELE------AAIQPDTSLVSVMTVNNE 202

Query: 208 --IKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGL 265
             +K+  ++I    +C   +     DAA+    +P D++   +D +++S +KL+G P G+
Sbjct: 203 IGVKQPIAEI--RQICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKLYG-PKGV 259

Query: 266 GALVVR 271
           GA+ +R
Sbjct: 260 GAIYIR 265


>ISCS_BUCBP (Q89A19) Cysteine desulfurase (EC 2.8.1.7)
          Length = 404

 Score = 52.8 bits (125), Expect = 3e-06
 Identities = 67/254 (26%), Positives = 109/254 (42%), Gaps = 29/254 (11%)

Query: 38  VYLDHAGATLYSELQMESVFKDLTTN-VYGNPHSQSDSSA-ATHDIVRDARQQVLDYCNA 95
           +YLD+A  T      M+ +   LT    +GNP S+S        + V  AR Q+ +  +A
Sbjct: 5   IYLDYAATTPVEFEVMKEMMNYLTLEGEFGNPASRSHKFGWKAEEAVDIARNQIAELIHA 64

Query: 96  SPEDYKCIFTSGATAA--LKLVGEAFPWSCNSNFMYTMEN-HNSVLGIREYALGQGAAAI 152
              +   IFTSGAT +  L + G A  +    N + T    H + L    Y   +G    
Sbjct: 65  DSREI--IFTSGATESNNLAIKGIAEFYKKKGNHIITCSTEHKATLDTCRYLENKG---- 118

Query: 153 AVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKIIKEDS 212
             DI  ++P   G      I   QE+ +K T L          S +  + ++  I  +D 
Sbjct: 119 -FDITYLNPLQNGT---INIYELQEKIQKNTIL---------VSIMHVNNEIGVI--QDI 163

Query: 213 SKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGLGALVVRN 272
            KI  + +C+    L  +DAA+    +  +L +  +D ++ S +K++G P G+G L +R 
Sbjct: 164 HKI--SKICQSNNILFHVDAAQSIGKININLKQLKIDLMSFSAHKIYG-PKGIGGLYIRR 220

Query: 273 DAAKLLKKSYFSGG 286
                L      GG
Sbjct: 221 KPRVRLSAQIHGGG 234


>CSD_PYRAB (Q9V242) Probable cysteine desulfurase (EC 2.8.1.7)
          Length = 401

 Score = 52.0 bits (123), Expect = 6e-06
 Identities = 67/319 (21%), Positives = 130/319 (40%), Gaps = 54/319 (16%)

Query: 35  QDLVYLDHAGATLYSELQMESV---FKDLTTNVYGNPHSQSDSSAATHDIVRDARQQVLD 91
           Q+++Y D+   +L  +  +E++   +     NV+   H  S  +   ++   ++R+ V D
Sbjct: 15  QEVIYFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYE---ESRKVVAD 71

Query: 92  YCNASPEDYKCIFTSGATAALKLV--GEAFPWSCNSNFMYT-MENHNSVLGIREYALGQG 148
           + NA  ++    FT   + +L LV  G    +      + T  E+H+++L  +  A  +G
Sbjct: 72  FINAEFDEIA--FTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKG 129

Query: 149 AAAIAV--------DIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRF 200
                +        D+ D   +I+G     K+   Q     +  + E E           
Sbjct: 130 LKLEFIEGDDEGNLDLADAEKKIKG----AKLVAVQHVSNALGVIHEVE----------- 174

Query: 201 DLDLAKIIKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFG 260
             +L K++KE+ +             + ++DAA+    M  D+ K   DF+A S +K   
Sbjct: 175 --ELGKMVKEEGA-------------IFVVDAAQSVGHMEVDVKKLKADFLAFSGHKGPM 219

Query: 261 YPTGLGALVVRNDAAKLLKKSYFSGGTVAASIADIDF-IKRREGIEELFEDGTVSFLSIA 319
            PTG+G L +  +   + +     GGT    I D++    +     E FE GT +     
Sbjct: 220 GPTGIGVLYINKEFFDVFEPPLIGGGT----IEDVELCCYKLTEPPERFEAGTPNIGGAI 275

Query: 320 SIRHGFKILNSLTVSAISR 338
            +  G K +  + +  I +
Sbjct: 276 GLAAGIKYIEKIGIDKIEK 294


>CSD_STAAN (P99177) Probable cysteine desulfurase (EC 2.8.1.7)
          Length = 413

 Score = 50.4 bits (119), Expect = 2e-05
 Identities = 70/314 (22%), Positives = 131/314 (41%), Gaps = 46/314 (14%)

Query: 37  LVYLDHAGATLYSELQ----MESVFKDLTTNVYGNPHSQSDSSAATHDIVRDARQQVLDY 92
           L YLD   AT  + +Q    +E  +K   +NV+   H+    S AT D   +AR+ V  +
Sbjct: 27  LAYLDST-ATSQTPMQVLNVLEDYYKRYNSNVHRGVHTLG--SLAT-DGYENARETVRRF 82

Query: 93  CNASPEDYKCIFTSGATAALKLV----GEAFPWSCNSNFMYTMENHNSVLGIREYALGQG 148
            NA   + + IFT G TA++ LV    G+A     +   +  ME+H +++  ++ A  + 
Sbjct: 83  INAKYFE-EIIFTRGTTASINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKN 141

Query: 149 AAAIAVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKII 208
           A      ++ +    +GE     I      + K+  +        + S +   ++  K I
Sbjct: 142 AT-----LKFIPMTADGELNIEDIKQTINDKTKIVAI-------AHISNVLGTINDVKTI 189

Query: 209 KEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGLGAL 268
                    A +  +   ++ +D A+ +  M  D+ +   DF + S +K+ G PTG+G L
Sbjct: 190 ---------AEIAHQHGAIISVDGAQAAPHMKLDMQEMNADFYSFSGHKMLG-PTGIGVL 239

Query: 269 VVRNDAAKLLKKSYFSGGTVAASIADIDFIKRREG----IEELFEDGTVSFLSIASIRHG 324
             + +  + ++   F G         IDF+ + +     +   FE GT        +   
Sbjct: 240 FGKRELLQKMEPIEFGGDM-------IDFVSKYDATWADLPTKFEAGTPLIAQAIGLAEA 292

Query: 325 FKILNSLTVSAISR 338
            + L  +   AI +
Sbjct: 293 IRYLERIGFDAIHK 306


>CSD_STAAM (P63518) Probable cysteine desulfurase (EC 2.8.1.7)
          Length = 413

 Score = 50.4 bits (119), Expect = 2e-05
 Identities = 70/314 (22%), Positives = 131/314 (41%), Gaps = 46/314 (14%)

Query: 37  LVYLDHAGATLYSELQ----MESVFKDLTTNVYGNPHSQSDSSAATHDIVRDARQQVLDY 92
           L YLD   AT  + +Q    +E  +K   +NV+   H+    S AT D   +AR+ V  +
Sbjct: 27  LAYLDST-ATSQTPMQVLNVLEDYYKRYNSNVHRGVHTLG--SLAT-DGYENARETVRRF 82

Query: 93  CNASPEDYKCIFTSGATAALKLV----GEAFPWSCNSNFMYTMENHNSVLGIREYALGQG 148
            NA   + + IFT G TA++ LV    G+A     +   +  ME+H +++  ++ A  + 
Sbjct: 83  INAKYFE-EIIFTRGTTASINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKN 141

Query: 149 AAAIAVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKII 208
           A      ++ +    +GE     I      + K+  +        + S +   ++  K I
Sbjct: 142 AT-----LKFIPMTADGELNIEDIKQTINDKTKIVAI-------AHISNVLGTINDVKTI 189

Query: 209 KEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGLGAL 268
                    A +  +   ++ +D A+ +  M  D+ +   DF + S +K+ G PTG+G L
Sbjct: 190 ---------AEIAHQHGAIISVDGAQAAPHMKLDMQEMNADFYSFSGHKMLG-PTGIGVL 239

Query: 269 VVRNDAAKLLKKSYFSGGTVAASIADIDFIKRREG----IEELFEDGTVSFLSIASIRHG 324
             + +  + ++   F G         IDF+ + +     +   FE GT        +   
Sbjct: 240 FGKRELLQKMEPIEFGGDM-------IDFVSKYDATWADLPTKFEAGTPLIAQAIGLAEA 292

Query: 325 FKILNSLTVSAISR 338
            + L  +   AI +
Sbjct: 293 IRYLERIGFDAIHK 306


>ISC1_ARCFU (O30052) Probable cysteine desulfurase 1 (EC 2.8.1.7)
          Length = 382

 Score = 48.5 bits (114), Expect = 6e-05
 Identities = 56/236 (23%), Positives = 99/236 (41%), Gaps = 29/236 (12%)

Query: 39  YLDHAGATLYSELQMESVFKDLTTNVYGNPHSQSDSSAATHDIVRDARQQVLDYCNASPE 98
           Y D+A      E  +E++   +T + +GNP S         +++ +AR ++ +  N    
Sbjct: 3   YFDYASTKPVDERVVEAMLPYMTEH-FGNPSSVHSYGFKAREVIEEARGKISELINGGGG 61

Query: 99  DYKCIFTSGATAA--LKLVGEAFPWS-CNSNFMYTMENHNSVLGIREYALGQGAAAIAVD 155
           D   +FTSGAT +  L L+G A   +    + + +   H SV+   +Y   QG     + 
Sbjct: 62  D--IVFTSGATESNNLALIGYAMRNARKGKHVLVSSIEHMSVINPAKYLQKQGFEVEFIP 119

Query: 156 IEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKIIKEDSSKI 215
           + D +  ++ E    KI               E+ +  +      ++   + IKE S  I
Sbjct: 120 V-DKYGTVDLEFIEEKI--------------REDTILVSVQHANNEIGTIQPIKEISEII 164

Query: 216 LGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGLGALVVR 271
                   GR  + +DA      +  D+ K   D + +S   ++G P G+GAL VR
Sbjct: 165 -------NGRAALHVDATASLGQIEVDVEKIGADMLTISSNDIYG-PKGVGALWVR 212


>CSD_STAEP (Q8CTA4) Probable cysteine desulfurase (EC 2.8.1.7)
          Length = 413

 Score = 47.0 bits (110), Expect = 2e-04
 Identities = 68/310 (21%), Positives = 127/310 (40%), Gaps = 42/310 (13%)

Query: 37  LVYLDHAGATLYSELQMESVFKDLTTNVYGNPHS--QSDSSAATHDIVRDARQQVLDYCN 94
           L YLD   AT  + +Q+ +V  D       N H    +  S AT D   +AR+ V  + N
Sbjct: 27  LAYLDST-ATSQTPVQVLNVLDDYYKRYNSNVHRGVHTLGSLAT-DGYENARETVRRFIN 84

Query: 95  ASPEDYKCIFTSGATAALKLV----GEAFPWSCNSNFMYTMENHNSVLGIREYALGQGAA 150
           A   + + IFT G TA++ +V    G+A     +   +  ME+H +++  ++ A  + A 
Sbjct: 85  AKYFE-EIIFTRGTTASINIVAHSYGDANISEGDEIVVTEMEHHANIVPWQQLAKRKNAT 143

Query: 151 AIAVDIEDVHPRIEGEKFPTKISLHQEQRRKVTGLQEEEPMECNFSGLRFDLDLAKIIKE 210
                ++ +    +GE     I      + K+  +     +    + ++    +AKI  E
Sbjct: 144 -----LKFIPMTKDGELQLDDIKATINDKTKIVAIAHVSNVLGTINDVK---TIAKIAHE 195

Query: 211 DSSKILGASVCKKGRWLVLIDAAKGSATMPPDLSKYPVDFVALSFYKLFGYPTGLGALVV 270
             +             ++ +D A+ +  M  D+     DF + S +K+ G PTG+G L  
Sbjct: 196 HGA-------------VISVDGAQSAPHMALDMQDIDADFYSFSGHKMLG-PTGIGVLYG 241

Query: 271 RNDAAKLLKKSYFSGGTVAASIADIDFIKRREG----IEELFEDGTVSFLSIASIRHGFK 326
           + +  + ++   F G         IDF+ + +     +   FE GT        +     
Sbjct: 242 KRELLQNMEPVEFGGDM-------IDFVSKYDSTWADLPTKFEAGTPLIAQAIGLAEAIH 294

Query: 327 ILNSLTVSAI 336
            + +L  +AI
Sbjct: 295 YIENLGFNAI 304


>CSD_MYCPN (P75298) Probable cysteine desulfurase (EC 2.8.1.7)
          Length = 408

 Score = 46.2 bits (108), Expect = 3e-04
 Identities = 62/258 (24%), Positives = 108/258 (41%), Gaps = 27/258 (10%)

Query: 67  NPHSQS-DSSAATHDIVRDARQQVLDYCNASPEDYKCIFTSGATAALKLVGEAF-PWSCN 124
           NPH+++ D +     I+ + RQ V D+ N +    + IFTS AT ++ L      PW   
Sbjct: 53  NPHNKTPDLNNQIIAIIAETRQLVADWFNVTA--LEIIFTSSATESINLFAHGLKPWIKP 110

Query: 125 SNFMYTMENHNSVLGIREYALGQGAAAIAVDIEDVHPRIEGEKFPTKISLHQEQRRKVTG 184
            + +    + +S   +   AL +   A  V +E    +   + F + I+     + KV  
Sbjct: 111 GDEIVLKGDEHSANVLPWVALAKQTKARLVWVEKQANQSLEDTFKSLIN----PKTKVVA 166

Query: 185 LQEEEPMECNFSGLRFDL-DLAKIIKEDSSKILGASVCKKGRWLVLIDAAKGSATMPPDL 243
           +        N  G   D   +A  +K+ + K             V +DA +       D+
Sbjct: 167 ITATS----NLFGNSIDFAQIADYLKQVNPKAF-----------VAVDAVQTVQHKQIDI 211

Query: 244 SKYPVDFVALSFYKLFGYPTGLGALVVRNDAAKLLKKSYFSGGTVAASIADIDFIKRREG 303
           +K  +DF+A S +K +G PTGLG   ++      L+     GG +   I D D     + 
Sbjct: 212 AKTQIDFLAFSTHKFYG-PTGLGVAYIKKSLQPQLQPLKL-GGDIFTQI-DPDNTIHFKS 268

Query: 304 IEELFEDGTVSFLSIASI 321
               FE GT + ++I ++
Sbjct: 269 TPLKFEAGTPNIMAIYAL 286


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.322    0.138    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,990,548
Number of Sequences: 164201
Number of extensions: 3719965
Number of successful extensions: 7663
Number of sequences better than 10.0: 96
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 84
Number of HSP's that attempted gapping in prelim test: 7596
Number of HSP's gapped (non-prelim): 136
length of query: 764
length of database: 59,974,054
effective HSP length: 118
effective length of query: 646
effective length of database: 40,598,336
effective search space: 26226525056
effective search space used: 26226525056
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 70 (31.6 bits)