BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148532.5 + phase: 0 /pseudo
(343 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RD22_ARATH (Q08298) Dehydration-responsive protein RD22 precursor 243 4e-64
EA92_VICFA (P21747) Embryonic abundant protein USP92 precursor 92 1e-18
EA30_VICFA (P21745) Embryonic abundant protein VF30.1 precursor 92 2e-18
SVF3_VICFA (P09059) Unknown seed protein 30.1 precursor (VF30.1) 92 2e-18
EA87_VICFA (P21746) Embryonic abundant protein USP87 precursor 92 2e-18
LAC1_THACU (P56193) Laccase 1 precursor (EC 1.10.3.2) (Benzenedi... 32 3.0
TNP6_ENTFC (Q06238) Transposase for transposon Tn1546 31 5.2
>RD22_ARATH (Q08298) Dehydration-responsive protein RD22 precursor
Length = 392
Score = 243 bits (621), Expect = 4e-64
Identities = 160/396 (40%), Positives = 203/396 (50%), Gaps = 82/396 (20%)
Query: 10 LVATHATLPPELYWKSKLPTTQIPKAITDILHPIC*LNLYRLYNFIFTLMKG-------- 61
+VA A L PE YW + LP T IP + L+ L F FT K
Sbjct: 16 VVAIAADLTPERYWSTALPNTPIPNS------------LHNLLTFDFTDEKSTNVQVGKG 63
Query: 62 -------KAKCDSKICM**LT*KLIQLGVTSVDDGK--------ISVD------------ 94
K K S + + G VD GK +SV
Sbjct: 64 GVNVNTHKGKTGSGTAV------NVGKGGVRVDTGKGKPGGGTHVSVGSGKGHGGGVAVH 117
Query: 95 ------------GKISVNGKTRIVYDAAPIYF----------YENDANEAELHDNRNLAM 132
GK V TR + PIY Y A E +LHD+ N A+
Sbjct: 118 TGKPGKRTDVGVGKGGVTVHTR--HKGRPIYVGVKPGANPFVYNYAAKETQLHDDPNAAL 175
Query: 133 FLLEKDLHHGTKFNIQFTKTSDHG--PTFLPGDVANSIPFSSNKLENILNYFSIKQGSTE 190
F LEKDL G + N++F +G FLP A ++PF S K L FS++ GS E
Sbjct: 176 FFLEKDLVRGKEMNVRFNAEDGYGGKTAFLPRGEAETVPFGSEKFSETLKRFSVEAGSEE 235
Query: 191 SEIVKNTISECEAYGIKGEEKLCVTSLESMIDFTTLKLGN-NVDTVSTEVNGESG-LQQY 248
+E++K TI ECEA + GEEK C TSLESM+DF+ KLG +V VSTEV ++ +Q+Y
Sbjct: 236 AEMMKKTIEECEARKVSGEEKYCATSLESMVDFSVSKLGKYHVRAVSTEVAKKNAPMQKY 295
Query: 249 VIAN-GVKKMGENNLVVCHKRNYPYAVFYCHKTDATKVYSVPLEGADGSRVKAVAICHSD 307
IA GVKK+ ++ VVCHK+ YP+AVFYCHK T VY+VPLEG +G R KAVA+CH +
Sbjct: 296 KIAAAGVKKLSDDKSVVCHKQKYPFAVFYCHKAMMTTVYAVPLEGENGMRAKAVAVCHKN 355
Query: 308 TSQWSLKHLAFQVLKVQQGTFPVCHILQQGQVVWFS 343
TS W+ HLAF+VLKV+ GT PVCH L + VVWFS
Sbjct: 356 TSAWNPNHLAFKVLKVKPGTVPVCHFLPETHVVWFS 391
>EA92_VICFA (P21747) Embryonic abundant protein USP92 precursor
Length = 268
Score = 92.4 bits (228), Expect = 1e-18
Identities = 62/183 (33%), Positives = 97/183 (52%), Gaps = 12/183 (6%)
Query: 166 NSIPFSSNKLENILNYFS-IKQGSTESEIVKNTISE-----CEAYGIKGEEKLCVTSLES 219
N I ++N + +I+ F+ +QG T+S + N + C + E K CV+SL+S
Sbjct: 79 NFILGNTNSVGSIIRPFTKSRQGVTDSIWLANKEKQSFEDFCYSPTAIAEHKHCVSSLKS 138
Query: 220 MIDFTTLKLGNN-VDTVSTEVNGESGLQQYVIANGVKKMGENNLVVCHKRNYPYAVFYCH 278
MID G+ + +S+ N QYV+ + VKK+G+N V+CH+ N+ VF CH
Sbjct: 139 MIDQVISHFGSTKIKAISS--NFAPYQDQYVVED-VKKVGDN-AVMCHRLNFEKVVFNCH 194
Query: 279 KTDATKVYSVPLEGADGSRVKAVAICHSDTSQWSLKHLAFQVLKVQQGTFPVCHILQQGQ 338
+ T Y V L +DG++ KA+ +CH DT + L ++ L+V GT PVCH +
Sbjct: 195 QVRETTAYVVSLVASDGTKTKALTVCHHDTRGMN-PELLYEALEVTPGTVPVCHFIGNKA 253
Query: 339 VVW 341
W
Sbjct: 254 AAW 256
Score = 33.5 bits (75), Expect = 0.80
Identities = 16/35 (45%), Positives = 20/35 (56%)
Query: 8 LALVATHATLPPELYWKSKLPTTQIPKAITDILHP 42
LA V AT E YW+S P T +PK +D+L P
Sbjct: 14 LAFVGITATSSEEDYWQSIWPNTPLPKTFSDLLIP 48
>EA30_VICFA (P21745) Embryonic abundant protein VF30.1 precursor
Length = 268
Score = 92.0 bits (227), Expect = 2e-18
Identities = 62/183 (33%), Positives = 97/183 (52%), Gaps = 12/183 (6%)
Query: 166 NSIPFSSNKLENILNYFS-IKQGSTESEIVKNTISE-----CEAYGIKGEEKLCVTSLES 219
N I ++N + +I+ F+ +QG T+S + N + C + E K CV+SL+S
Sbjct: 79 NFILGNTNSVGSIIRPFTKSRQGVTDSIWLANKEKQSLEDFCYSPTAIAEHKHCVSSLKS 138
Query: 220 MIDFTTLKLGNN-VDTVSTEVNGESGLQQYVIANGVKKMGENNLVVCHKRNYPYAVFYCH 278
MID G+ + +S+ N QYV+ + VKK+G+N V+CH+ N+ VF CH
Sbjct: 139 MIDQVISHFGSTKIKAISS--NFAPYQDQYVVED-VKKVGDN-AVMCHRLNFEKVVFNCH 194
Query: 279 KTDATKVYSVPLEGADGSRVKAVAICHSDTSQWSLKHLAFQVLKVQQGTFPVCHILQQGQ 338
+ T Y V L +DG++ KA+ +CH DT + L ++ L+V GT PVCH +
Sbjct: 195 QVRDTTAYVVSLVASDGTKTKALTVCHHDTRGMN-PELLYEALEVTPGTVPVCHFIGNKA 253
Query: 339 VVW 341
W
Sbjct: 254 AAW 256
Score = 33.1 bits (74), Expect = 1.0
Identities = 16/35 (45%), Positives = 20/35 (56%)
Query: 8 LALVATHATLPPELYWKSKLPTTQIPKAITDILHP 42
LA V AT E YW+S P T +PK +D+L P
Sbjct: 14 LAFVGITATSSGEDYWQSIWPNTPLPKTFSDLLIP 48
>SVF3_VICFA (P09059) Unknown seed protein 30.1 precursor (VF30.1)
Length = 268
Score = 91.7 bits (226), Expect = 2e-18
Identities = 62/183 (33%), Positives = 97/183 (52%), Gaps = 12/183 (6%)
Query: 166 NSIPFSSNKLENILNYFS-IKQGSTESEIVKNTISE-----CEAYGIKGEEKLCVTSLES 219
N I ++N + +I+ F+ +QG T+S + N + C + E K CV+SL+S
Sbjct: 79 NFILGNTNSVGSIIRPFTKSRQGVTDSIWLANKEKQSLEDFCYSPTAIAEHKHCVSSLKS 138
Query: 220 MIDFTTLKLGNN-VDTVSTEVNGESGLQQYVIANGVKKMGENNLVVCHKRNYPYAVFYCH 278
MID G+ + +S+ N QYV+ + VKK+G+N V+CH+ N+ VF CH
Sbjct: 139 MIDQVISHFGSTKIKAISS--NFAPYQDQYVVED-VKKVGDN-AVMCHRLNFEKVVFNCH 194
Query: 279 KTDATKVYSVPLEGADGSRVKAVAICHSDTSQWSLKHLAFQVLKVQQGTFPVCHILQQGQ 338
+ T Y V L +DG++ KA+ +CH DT + L ++ L+V GT PVCH +
Sbjct: 195 QVRDTTAYVVSLVASDGTKTKALTVCHHDTRGMN-PELLYEALEVTLGTVPVCHFIGNKA 253
Query: 339 VVW 341
W
Sbjct: 254 AAW 256
Score = 30.8 bits (68), Expect = 5.2
Identities = 15/35 (42%), Positives = 19/35 (53%)
Query: 8 LALVATHATLPPELYWKSKLPTTQIPKAITDILHP 42
LA V AT E YW+S P T +PK +D+ P
Sbjct: 14 LAFVGITATSSGEDYWQSIWPNTPLPKTFSDLSIP 48
>EA87_VICFA (P21746) Embryonic abundant protein USP87 precursor
Length = 268
Score = 91.7 bits (226), Expect = 2e-18
Identities = 62/183 (33%), Positives = 97/183 (52%), Gaps = 12/183 (6%)
Query: 166 NSIPFSSNKLENILNYFS-IKQGSTESEIVKNTISE-----CEAYGIKGEEKLCVTSLES 219
N I ++N + +I+ F+ +QG T+S + N + C + E K CV+SL+S
Sbjct: 79 NFILGNTNSVGSIIRPFTKSRQGVTDSIWLANKEKQSLEDFCYSPTAIAEHKHCVSSLKS 138
Query: 220 MIDFTTLKLGNN-VDTVSTEVNGESGLQQYVIANGVKKMGENNLVVCHKRNYPYAVFYCH 278
MID G+ + +S+ N QYV+ + VKK+G+N V+CH+ N+ VF CH
Sbjct: 139 MIDQVISHFGSTKIKAISS--NFAPYQDQYVVED-VKKVGDN-AVMCHRLNFEKVVFNCH 194
Query: 279 KTDATKVYSVPLEGADGSRVKAVAICHSDTSQWSLKHLAFQVLKVQQGTFPVCHILQQGQ 338
+ T Y V L +DG++ KA+ +CH DT + L ++ L+V GT PVCH +
Sbjct: 195 QVRDTTAYVVSLVASDGTKTKALTVCHHDTRGMN-PELLYEALEVTLGTVPVCHFIGNKA 253
Query: 339 VVW 341
W
Sbjct: 254 AAW 256
Score = 35.8 bits (81), Expect = 0.16
Identities = 17/35 (48%), Positives = 21/35 (59%)
Query: 8 LALVATHATLPPELYWKSKLPTTQIPKAITDILHP 42
LA V AT P E YW+S P T +PK +D+L P
Sbjct: 14 LAFVGITATSPREDYWQSIWPNTPLPKTFSDMLIP 48
>LAC1_THACU (P56193) Laccase 1 precursor (EC 1.10.3.2)
(Benzenediol:oxygen oxidoreductase) (Urishiol oxidase)
(Diphenol oxidase)
Length = 576
Score = 31.6 bits (70), Expect = 3.0
Identities = 21/74 (28%), Positives = 37/74 (49%), Gaps = 8/74 (10%)
Query: 166 NSIPFSSNKLENILNYFSIKQGSTESEIVK--NTI----SECEAYGIKGEEKLCVTSLES 219
N IP+ S K+ +L + + G TES+ K +T+ ++C + IKG + +T
Sbjct: 415 NGIPYESPKIPTLLKILTDEDGVTESDFTKEEHTVILPKNKCIEFNIKGNSGIPITHPVH 474
Query: 220 MIDFT--TLKLGNN 231
+ T ++ GNN
Sbjct: 475 LHGHTWDVVQFGNN 488
>TNP6_ENTFC (Q06238) Transposase for transposon Tn1546
Length = 988
Score = 30.8 bits (68), Expect = 5.2
Identities = 35/141 (24%), Positives = 60/141 (41%), Gaps = 22/141 (15%)
Query: 82 GVTSVDDGKISVDGKISVNGKTRIVYDA---APIYFYENDANEAE----LHDNRNLAMFL 134
G TS DG G S++ Y A IY + +D + +H N A+ +
Sbjct: 679 GTTSSSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHV 738
Query: 135 LEKDLHHGTKFNIQ--FTKTSDH-----GPTFLPG--------DVANSIPFSSNKLENIL 179
L+ LHH T NI+ +T T+ + G T L G D+++S F+ +K
Sbjct: 739 LDGLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP 798
Query: 180 NYFSIKQGSTESEIVKNTISE 200
+I +G ++++K +
Sbjct: 799 KLEAILRGQINTKVIKENYED 819
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.140 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,234,271
Number of Sequences: 164201
Number of extensions: 1591540
Number of successful extensions: 4779
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 4759
Number of HSP's gapped (non-prelim): 12
length of query: 343
length of database: 59,974,054
effective HSP length: 111
effective length of query: 232
effective length of database: 41,747,743
effective search space: 9685476376
effective search space used: 9685476376
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 66 (30.0 bits)
Medicago: description of AC148532.5