BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC148532.2 - phase: 0 
         (356 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YLE1_SCHPO (Q10451) Hypothetical protein C1093.01 in chromosome I      46  2e-04
YMV6_YEAST (Q04748) Hypothetical 104.7 kDa protein in NCA1-HMS1 ...    33  1.1
RPM2_YEAST (Q02773) Ribonuclease P protein component, mitochondr...    32  1.9
CFTR_MOUSE (P26361) Cystic fibrosis transmembrane conductance re...    32  3.2
VI07_VARV (P33003) Protein I7                                          31  4.2
VPS3_YEAST (P23643) Vacuolar protein sorting-associated protein ...    31  5.5
RPOM_YEAST (P13433) DNA-directed RNA polymerase, mitochondrial p...    31  5.5
PRIA_RICPR (Q9ZD10) Primosomal protein N' (Replication factor Y)       30  7.1
POLG_BSTV1 (Q65730) Genome polyprotein [Contains: P1 proteinase ...    30  7.1
VI07_VACCV (P12926) Protein I7                                         30  9.3
VI07_VACCC (P20501) Protein I7                                         30  9.3
V096_FOWPV (Q9J5C4) Protein FPV096                                     30  9.3
SECA_BUCBP (Q89AR1) Preprotein translocase secA subunit                30  9.3

>YLE1_SCHPO (Q10451) Hypothetical protein C1093.01 in chromosome I
          Length = 1261

 Score = 45.8 bits (107), Expect = 2e-04
 Identities = 38/154 (24%), Positives = 69/154 (44%), Gaps = 4/154 (2%)

Query: 22   ILEKLLKENVKDHG-RFSELIFLCGKLKNVQLGINVFTSMEGVGVKPTSLVFNSLISACL 80
            I E+  + NVK     ++ ++   G+ +       +F  M+  G+ PTS+ + ++I+A  
Sbjct: 913  IFEETKRHNVKPSVFLYNAVLSKLGRARRTTECWKLFQEMKESGLLPTSVTYGTVINAAC 972

Query: 81   SSHDIVTAYSLFEIMESSENYKPDFHTYNNFIS-AFSKSGNVDAMLAWYSAKKATGLGPD 139
               D   A  LF  ME+  NY+P    YN  I        N +  L +Y+   AT + P 
Sbjct: 973  RIGDESLAEKLFAEMENQPNYQPRVAPYNTMIQFEVQTMFNREKALFYYNRLCATDIEPS 1032

Query: 140  LQTFESVIS--GCVNSKNYEIADRVFEEMKISEM 171
              T++ ++   G +   N      V E M+ +++
Sbjct: 1033 SHTYKLLMDAYGTLKPVNVGSVKAVLELMERTDV 1066



 Score = 31.2 bits (69), Expect = 4.2
 Identities = 44/194 (22%), Positives = 75/194 (37%), Gaps = 36/194 (18%)

Query: 72  FNSLISACLSSH----------DIVTAYSLFEIMESSENYKPDFHTYNNFISAFSKSGNV 121
           FNSL ++ LS+           D +T  SL     S       F + N FI    +  + 
Sbjct: 136 FNSLATSPLSTETQSEVSDPLLDTLTPNSLESSFASIS--LNSFTSANEFIQFLKRLASS 193

Query: 122 DAMLAWYSAKKATGLGPDLQTFES--VISGCVN--------SKNYEIADRVFEEMKISEM 171
              +  +   K     P+L T E+  ++  C++        SKN +   +V+ +M  S +
Sbjct: 194 KLSIHTFDLYKLVRNSPELLTLEAYNIVLQCMSTDDYFLSSSKNIQKIIKVYVDMLNSFI 253

Query: 172 IPNVTILESMLKGFCSQKSLCRANEFFKFVLDNRWQISETMAAMLVVLYHEQGQVEKMEE 231
            PNVT  E+++   C +          KFV      +S+       +  H     E   E
Sbjct: 254 SPNVTTFETVIFALCRRA---------KFVHQKIESLSKR-----TIYAHPSIAKEIQPE 299

Query: 232 LLETITSYPIDSGV 245
           LL+  +  P+ + V
Sbjct: 300 LLDLQSEMPLQTAV 313



 Score = 30.4 bits (67), Expect = 7.1
 Identities = 18/76 (23%), Positives = 35/76 (45%), Gaps = 1/76 (1%)

Query: 45  GKLKNVQLGINVFTSMEGVGVKPTSLVFNSLISACLSSHDIVTAYSLFEIMESSENYKPD 104
           G   +    +N+F   +   VKP+  ++N+++S    +      + LF+ M+ S    P 
Sbjct: 902 GDTDDATTALNIFEETKRHNVKPSVFLYNAVLSKLGRARRTTECWKLFQEMKES-GLLPT 960

Query: 105 FHTYNNFISAFSKSGN 120
             TY   I+A  + G+
Sbjct: 961 SVTYGTVINAACRIGD 976



 Score = 30.0 bits (66), Expect = 9.3
 Identities = 31/146 (21%), Positives = 63/146 (42%), Gaps = 18/146 (12%)

Query: 65   VKPTSLVFNSLISA--CLSSHDIVTAYSLFEIMESSE------NYKPDFHTYNNFISAFS 116
            ++P+S  +  L+ A   L   ++ +  ++ E+ME ++      +Y    H   N +S   
Sbjct: 1029 IEPSSHTYKLLMDAYGTLKPVNVGSVKAVLELMERTDVPILSMHYAAYIHILGNVVS--- 1085

Query: 117  KSGNVDAMLAWYS---AKKATG-LGPDLQTFESVISGCVNSKNYEIADRVFEEMKISEMI 172
               +V A  + Y    AK   G +  D   F+S I   + +       ++  +MK   + 
Sbjct: 1086 ---DVQAATSCYMNALAKHDAGEIQLDANLFQSQIESLIANDRIVEGIQIVSDMKRYNVS 1142

Query: 173  PNVTILESMLKGFCSQKSLCRANEFF 198
             N  I+ +++KGF     + +A  +F
Sbjct: 1143 LNAYIVNALIKGFTKVGMISKARYYF 1168


>YMV6_YEAST (Q04748) Hypothetical 104.7 kDa protein in NCA1-HMS1
           intergenic region
          Length = 898

 Score = 33.1 bits (74), Expect = 1.1
 Identities = 31/142 (21%), Positives = 61/142 (42%), Gaps = 14/142 (9%)

Query: 72  FNSLISACLSSHDIVTAYSLFEIMESSENYK---PDFHTYNNFISAFSKSGNVDAMLAWY 128
           FN L++  L + +   AY++ + +E +       PD   YN F S  +         A++
Sbjct: 538 FNGLLNILLGTGEEEKAYNILKKLEEASKKSKTDPDL-LYNQFYSEVN---------AYH 587

Query: 129 SAKKATGLGPDLQTFESVISGCVNSKNY-EIADRVFEEMKISEMIPNVTILESMLKGFCS 187
            AK        +Q  ++  +     K + +    + + M+ SE+IPN   L  +L  + S
Sbjct: 588 YAKFVEFYSLQIQNMKAQNTPSFRKKEFKQKVKSLLKRMQESEVIPNAVFLREILNFYDS 647

Query: 188 QKSLCRANEFFKFVLDNRWQIS 209
                 + E    +L+++ Q+S
Sbjct: 648 MYDFNSSFEIINPLLESKQQVS 669



 Score = 31.2 bits (69), Expect = 4.2
 Identities = 38/184 (20%), Positives = 80/184 (42%), Gaps = 16/184 (8%)

Query: 69  SLVFNSLISACLSSHDIVTAYSLFEIMESSENYKPDFHTYNNFISAFSKSGNVD---AML 125
           S +   LI   +  H       LF++   +    PD       +S F KSG+++   A+L
Sbjct: 718 SSIVKKLIKRQIKIHPTCHPRVLFQMTAENGEILPDKTFSKLIVSTFMKSGDLEAIPAIL 777

Query: 126 AWYSAKKATGLGPDLQTFESVISGCVNSKNYEIA----DRVFEEMKISEMIPNVTILESM 181
            + + K    +  DL  +  ++ G       +I+    D    +++ +E++ N +IL+++
Sbjct: 778 TFLTKKFDLNIDYDLSMY--ILKGLKRQYLRDISNISKDACEYKLRKAELMNNESILKNI 835

Query: 182 LKGFCSQKSLCR-ANEFFKFVLDNRWQISETMAAMLVV--LYHEQG-QVEKMEELLETIT 237
            +G   + ++     E   F+   +W+     +  L+V   + E G +   +EEL+E + 
Sbjct: 836 PQGTNQENTISHLIREILIFI---KWKEKSDCSTFLMVEDAFKELGTEFTLLEELIEDVN 892

Query: 238 SYPI 241
              I
Sbjct: 893 KLKI 896


>RPM2_YEAST (Q02773) Ribonuclease P protein component, mitochondrial
           precursor (EC 3.1.26.5) (RNase P)
          Length = 1202

 Score = 32.3 bits (72), Expect = 1.9
 Identities = 13/49 (26%), Positives = 26/49 (52%)

Query: 140 LQTFESVISGCVNSKNYEIADRVFEEMKISEMIPNVTILESMLKGFCSQ 188
           LQT    I+ C   KNY   + +++ +K ++++P + I   +L   C +
Sbjct: 187 LQTHIDEINRCYEQKNYNKINSLYQSLKRNDIVPPLEIFTKVLDSLCKR 235


>CFTR_MOUSE (P26361) Cystic fibrosis transmembrane conductance
           regulator (CFTR) (cAMP-dependent chloride channel)
          Length = 1476

 Score = 31.6 bits (70), Expect = 3.2
 Identities = 24/76 (31%), Positives = 37/76 (48%), Gaps = 2/76 (2%)

Query: 208 ISETMAAMLVVLYHEQGQVEKMEELLETITSYPIDSGVLSQIHCGIVTMYAMLDRLDEVE 267
           I + +   ++V Y +Q   +  E L+  ITS  ID+    + +C    M  M++ L EVE
Sbjct: 235 IFQAILGKMMVKYRDQRAAKINERLV--ITSEIIDNIYSVKAYCWESAMEKMIENLREVE 292

Query: 268 LSVGRMLKQGMSFTSS 283
           L + R       FTSS
Sbjct: 293 LKMTRKAAYMRFFTSS 308


>VI07_VARV (P33003) Protein I7
          Length = 423

 Score = 31.2 bits (69), Expect = 4.2
 Identities = 34/141 (24%), Positives = 56/141 (39%), Gaps = 13/141 (9%)

Query: 79  CLSSHDIVTAYS----LFEIMESSENYKPDFHTYNNF-ISAFSKSGNVDAMLAWYSAKKA 133
           C  SH     Y     L    +S  N   +FH YNNF   +FS   N +     +S    
Sbjct: 237 CYLSHWKCVIYDKKQCLVSFYDSGGNIPTEFHHYNNFYFYSFSDGFNTNHR---HSVLDN 293

Query: 134 TGLGPDL--QTFESVISGCVNSKNYEIADRVFEEMKISEMIPNVTILESMLKGFCSQKSL 191
           T    D+  + FE +    +   N E+   +  E  +   +  +    +  K F   KSL
Sbjct: 294 TNCDIDVLFRFFECIFGAKIGCINVEVNQLLESECGMFISLFMILCTRTPPKSF---KSL 350

Query: 192 CRANEFFKFVLDNRWQISETM 212
            +   FFKF+ D +  + +++
Sbjct: 351 KKVYTFFKFLADKKMTLFKSI 371


>VPS3_YEAST (P23643) Vacuolar protein sorting-associated protein VPS3
          Length = 1011

 Score = 30.8 bits (68), Expect = 5.5
 Identities = 36/150 (24%), Positives = 65/150 (43%), Gaps = 25/150 (16%)

Query: 68   TSLVFNSLISACLSSHDIVTAYSLFEIMESSENYKPDFHTYNNFISAFSKSGNVDAMLAW 127
            T L F + ++  +SS +++  Y  F    S E Y        N ++   +  ++ ++   
Sbjct: 876  TLLFFPNELTNWVSSEELLKIYLSFNDFRSVEKYIGK----QNLVAVMKQYLDISSLN-- 929

Query: 128  YSAKKATGLGPDLQTFESVISGCVNSKNYEIADRVFEEMKISEMIPNV----TILESMLK 183
            YS +  T L   LQ            +N+E+ D    ++KI E IP+V    TI E +LK
Sbjct: 930  YSVELVTNL---LQ------------RNFELLDDTDIQLKILETIPSVFPVQTISELLLK 974

Query: 184  GFCSQKSLCRANEFFKFVLDNRWQISETMA 213
                 +     +   K +L N+  IS+ ++
Sbjct: 975  VLIKYQEKKEESNLRKCLLKNQISISDELS 1004


>RPOM_YEAST (P13433) DNA-directed RNA polymerase, mitochondrial
           precursor (EC 2.7.7.6)
          Length = 1351

 Score = 30.8 bits (68), Expect = 5.5
 Identities = 16/46 (34%), Positives = 28/46 (60%), Gaps = 1/46 (2%)

Query: 74  SLISACLSSHDIVTAYSLFEIMESSENYKPDF-HTYNNFISAFSKS 118
           SL+ ACL S+ +  A+S+ E +     +K  F   YN ++++FSK+
Sbjct: 116 SLLEACLQSNLMKRAFSILESLYLVPEHKQRFIEDYNMYLNSFSKN 161


>PRIA_RICPR (Q9ZD10) Primosomal protein N' (Replication factor Y)
          Length = 648

 Score = 30.4 bits (67), Expect = 7.1
 Identities = 34/152 (22%), Positives = 66/152 (43%), Gaps = 24/152 (15%)

Query: 32  KDHGRFSELIF-LCGKLKNVQLGINVFTSMEGVGVKPTSLVFNSLISACLSSH--DIVTA 88
           KDH +  E I  L  +++N+++ I + T +   G    +L    +I A L S+  ++  +
Sbjct: 435 KDHAKTPEKIAQLLHQMENLEIDILIGTQIITKGYHFPNLTLVGVIDADLGSNNAELRAS 494

Query: 89  YSLFEIMESS---------------ENYKPDFHTYNNFISAFSKSGNVDAMLA-WYSAKK 132
              F+++                  ++Y PD     N I ++ K G+ D         +K
Sbjct: 495 ERTFQLLHQVGGRAGRGDSKGVVYLQSYYPD-----NIIFSYVKVGDEDRFFTNELEIRK 549

Query: 133 ATGLGPDLQTFESVISGCVNSKNYEIADRVFE 164
           A  + P  +T   ++SG   SK  +IA ++ +
Sbjct: 550 AANMPPFSKTASLILSGFSESKILDIARKIVQ 581


>POLG_BSTV1 (Q65730) Genome polyprotein [Contains: P1 proteinase
            (N-terminal protein); Helper component proteinase (EC
            3.4.22.45) (HC-pro); Protein P3; 6 kDa protein 1 (6K1);
            Cytoplasmic inclusion protein (CI); 6 kDa protein 2
            (6K2); Viral genome-linked p
          Length = 3093

 Score = 30.4 bits (67), Expect = 7.1
 Identities = 26/109 (23%), Positives = 50/109 (45%), Gaps = 9/109 (8%)

Query: 8    EILIINILIQVLEWILEKLLKENVKDHGRFSELIFLCGKLKNVQLG--------INVFTS 59
            +IL+  ++  +  W+L+KLLK    + GR +E++    K + + +         I+ F S
Sbjct: 1051 QILVAAVITAIGSWLLKKLLKFIRHEKGRLNEVVVFQSKQEELFISKFMAVCFVISTFFS 1110

Query: 60   MEGVGVKPTSLVFNSLISACLSSHDIVTAYSLFEIMESSENYKPDFHTY 108
            ++      +SL     I + LS   I  + +L E +E    +   FH +
Sbjct: 1111 LDMSNAIYSSLTKFRAIFSILSVGSIYQSGAL-EKLEEQLGHVDTFHEF 1158


>VI07_VACCV (P12926) Protein I7
          Length = 423

 Score = 30.0 bits (66), Expect = 9.3
 Identities = 34/141 (24%), Positives = 55/141 (38%), Gaps = 13/141 (9%)

Query: 79  CLSSHDIVTAYS----LFEIMESSENYKPDFHTYNNF-ISAFSKSGNVDAMLAWYSAKKA 133
           C  SH     Y     L    +S  N   +FH YNNF   +FS   N +     +S    
Sbjct: 237 CYLSHWKCVIYDKKQCLVSFYDSGGNIPTEFHHYNNFYFYSFSDGFNTNHK---HSVLDN 293

Query: 134 TGLGPDL--QTFESVISGCVNSKNYEIADRVFEEMKISEMIPNVTILESMLKGFCSQKSL 191
           T    D+  + FE      +   N E+   +  E  +   +  +    +  K F   KSL
Sbjct: 294 TNCDIDVLFRFFECTFGAKIGCINVEVNQLLESECGMFISLFMILCTRTPPKSF---KSL 350

Query: 192 CRANEFFKFVLDNRWQISETM 212
            +   FFKF+ D +  + +++
Sbjct: 351 KKVYTFFKFLADKKMTLFKSI 371


>VI07_VACCC (P20501) Protein I7
          Length = 423

 Score = 30.0 bits (66), Expect = 9.3
 Identities = 34/141 (24%), Positives = 55/141 (38%), Gaps = 13/141 (9%)

Query: 79  CLSSHDIVTAYS----LFEIMESSENYKPDFHTYNNF-ISAFSKSGNVDAMLAWYSAKKA 133
           C  SH     Y     L    +S  N   +FH YNNF   +FS   N +     +S    
Sbjct: 237 CYLSHWKCVIYDKKQCLVSFYDSGGNIPTEFHHYNNFYFYSFSDGFNTNHR---HSVLDN 293

Query: 134 TGLGPDL--QTFESVISGCVNSKNYEIADRVFEEMKISEMIPNVTILESMLKGFCSQKSL 191
           T    D+  + FE      +   N E+   +  E  +   +  +    +  K F   KSL
Sbjct: 294 TNCDIDVLFRFFECTFGAKIGCINVEVNQLLESECGMFISLFMILCTRTPPKSF---KSL 350

Query: 192 CRANEFFKFVLDNRWQISETM 212
            +   FFKF+ D +  + +++
Sbjct: 351 KKVYTFFKFLADKKMTLFKSI 371


>V096_FOWPV (Q9J5C4) Protein FPV096
          Length = 571

 Score = 30.0 bits (66), Expect = 9.3
 Identities = 61/268 (22%), Positives = 103/268 (37%), Gaps = 34/268 (12%)

Query: 1   MLLRFIIEILIINILIQVLEWILEKLLKENVKDHGRFSELIFLCGKLKNVQLGINVFTSM 60
           + L FI +    +I+++    +    L + VK++  F++ I +  +  N  L    F   
Sbjct: 63  IFLHFIRDCETYDIVLKSSFDVTLLYLNQLVKNYTSFTDFIDIYKQQSNTLLDDKRF--- 119

Query: 61  EGVGVKPTSLVFNSLISACLSSHDIVTAYSLFEIMESSENYKPDFHTYNNFISAFSKSGN 120
             + V   S  F  +IS   S+ ++   + L E ++  E           FI     S  
Sbjct: 120 --LFVTKLSPYFQDIISVNFST-ELNPLFHLNEPIKDLEIIYSKLFKETRFIKVDRIS-- 174

Query: 121 VDAMLAW-YSAKKATGLGPD----------LQTFESVISGCVNSKNYEIA---------- 159
           V  +L W YS K  TG+  D          LQ    V+S  +     E            
Sbjct: 175 VLRLLIWAYSLKMDTGMKFDDNDSHDLYTILQKTGPVVSSIMTETFKEFVFPKNSTTSYW 234

Query: 160 ----DRVFEEMKISEMIPNVTILESMLKGFCSQKSLCRANEFFKFVLDNRWQISETMAAM 215
               +R++ + K+    P +TI E +L    S+    R N+    V+      SE    M
Sbjct: 235 LFMKERIYNDEKVYTNEPAITIYEKVLSYIYSEIKQARVNKNMLKVVYMLDSDSEIKKFM 294

Query: 216 LVVLYHEQGQV-EKMEELLETITSYPID 242
           L ++Y   G +   ++E  ET  SY +D
Sbjct: 295 LELIYGIPGDILSIIDERDETWKSYFVD 322


>SECA_BUCBP (Q89AR1) Preprotein translocase secA subunit
          Length = 850

 Score = 30.0 bits (66), Expect = 9.3
 Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 7/55 (12%)

Query: 9   ILIINILIQVLEWILEKLLKENVKD-------HGRFSELIFLCGKLKNVQLGINV 56
           +L+  I IQ  E I +KLLK N+K        H + +++I   GKL+ V +  N+
Sbjct: 452 VLVGTISIQKSELISQKLLKLNIKHNVLNAKFHAQEAKIIAQAGKLRAVTIATNM 506


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.321    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,131,776
Number of Sequences: 164201
Number of extensions: 1200777
Number of successful extensions: 3281
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 3268
Number of HSP's gapped (non-prelim): 19
length of query: 356
length of database: 59,974,054
effective HSP length: 111
effective length of query: 245
effective length of database: 41,747,743
effective search space: 10228197035
effective search space used: 10228197035
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 66 (30.0 bits)