BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148485.6 + phase: 0
(380 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
GUNG_CLOCE (P37700) Endoglucanase G precursor (EC 3.2.1.4) (Endo... 33 1.2
ER12_BRANA (O65726) Squalene monooxygenase 1,2 (EC 1.14.99.7) (S... 33 1.6
YG2K_YEAST (P53253) Hypothetical 106.5 kDa protein in CTT1-PRP31... 32 2.7
ER11_BRANA (O65727) Squalene monooxygenase 1,1 (EC 1.14.99.7) (S... 32 2.7
TOP2_YEAST (P06786) DNA topoisomerase II (EC 5.99.1.3) 31 4.6
TIS1_DROME (P47980) TIS11 protein (dTIS11) 31 4.6
ER12_ARATH (O65402) Squalene monooxygenase 1,2 (EC 1.14.99.7) (S... 31 4.6
ER11_ARATH (O65404) Squalene monooxygenase 1,1 (EC 1.14.99.7) (S... 31 6.0
SECY_PAVLU (P28540) Preprotein translocase secY subunit 30 7.8
NPR1_YEAST (P22211) Nitrogen permease reactivator protein (EC 2.... 30 7.8
>GUNG_CLOCE (P37700) Endoglucanase G precursor (EC 3.2.1.4)
(Endo-1,4-beta-glucanase G) (Cellulase G) (EGCCG)
Length = 725
Score = 33.1 bits (74), Expect = 1.2
Identities = 34/124 (27%), Positives = 49/124 (39%), Gaps = 21/124 (16%)
Query: 98 AATLDRFGLKQSSSFVNSKNTTISNFSKLSGSSNV--VSEDHSGVHLATSGSNFIGAEAD 155
AA +D L SS++ KNT +S S+NV V+ D +G ++ G + E
Sbjct: 541 AAGIDPLSLVTSSNYSEGKNTKVSGVLPWDVSNNVYYVNVDLTGENIYPGGQSACRREVQ 600
Query: 156 INV--------WNPKVDLPDD--------STTAQIWLKAGNGNEFESIEAGWMVNPG--- 196
+ WNPK D D +T I + F + AG NP
Sbjct: 601 FRIAAPQGRRYWNPKNDFSYDGLPTTSTVNTVTNIPVYDNGVKVFGNEPAGGSENPDPEI 660
Query: 197 LYGN 200
LYG+
Sbjct: 661 LYGD 664
>ER12_BRANA (O65726) Squalene monooxygenase 1,2 (EC 1.14.99.7)
(Squalene epoxidase 1,2) (SE 1,2)
Length = 518
Score = 32.7 bits (73), Expect = 1.6
Identities = 19/44 (43%), Positives = 24/44 (54%), Gaps = 8/44 (18%)
Query: 207 SYW-----TTDSYKST---GCFDLTCSGFVQTSNTVALGGGINP 242
S+W +TD K GCFD SG +TS +AL GG+NP
Sbjct: 400 SFWQVLIASTDEAKEAMRQGCFDYLSSGGFRTSGLMALIGGMNP 443
>YG2K_YEAST (P53253) Hypothetical 106.5 kDa protein in CTT1-PRP31
intergenic region
Length = 936
Score = 32.0 bits (71), Expect = 2.7
Identities = 17/81 (20%), Positives = 33/81 (39%)
Query: 92 KQDLLRAATLDRFGLKQSSSFVNSKNTTISNFSKLSGSSNVVSEDHSGVHLATSGSNFIG 151
K D+ R L + + SSS NT + +S + +H + ++ +
Sbjct: 284 KPDVARVQGLQKMRISSSSSVSADSNTLEDQSPMIPNDPGGLSSSNQTIHPSQQNNSPVP 343
Query: 152 AEADINVWNPKVDLPDDSTTA 172
+ N+ NP P D+T++
Sbjct: 344 LSSHSNILNPAASYPTDATSS 364
>ER11_BRANA (O65727) Squalene monooxygenase 1,1 (EC 1.14.99.7)
(Squalene epoxidase 1,1) (SE 1,1)
Length = 506
Score = 32.0 bits (71), Expect = 2.7
Identities = 17/36 (47%), Positives = 21/36 (58%), Gaps = 3/36 (8%)
Query: 210 TTDSYKST---GCFDLTCSGFVQTSNTVALGGGINP 242
+TD K GCFD SG +TS +AL GG+NP
Sbjct: 407 STDEAKEAMRQGCFDYLSSGGFRTSGMMALLGGMNP 442
>TOP2_YEAST (P06786) DNA topoisomerase II (EC 5.99.1.3)
Length = 1428
Score = 31.2 bits (69), Expect = 4.6
Identities = 33/144 (22%), Positives = 59/144 (40%), Gaps = 19/144 (13%)
Query: 26 EIEAKLKLHNRPAVKTIKSENGDIIDCVDIYKQPAFDHPVLKNHTIQTWHKSGRCPKGTV 85
+I LK + +VK+ + +N I + + PAF + T + RC
Sbjct: 327 KISEILKKKKKKSVKSFQIKNNMFIFINCLIENPAFTSQTKEQLTTRVKDFGSRCEIPLE 386
Query: 86 PIRRIQKQDLLR-----AATLDRFGLKQSSSFVNSKNTTISNFSKLSGSSN--------- 131
I +I K DL A + LK+S ++ + I+N+ KL ++
Sbjct: 387 YINKIMKTDLATRMFEIADANEENALKKSD---GTRKSRITNYPKLEDANKAGTKEGYKC 443
Query: 132 --VVSEDHSGVHLATSGSNFIGAE 153
V++E S + LA +G +G +
Sbjct: 444 TLVLTEGDSALSLAVAGLAVVGRD 467
>TIS1_DROME (P47980) TIS11 protein (dTIS11)
Length = 437
Score = 31.2 bits (69), Expect = 4.6
Identities = 22/84 (26%), Positives = 34/84 (40%), Gaps = 1/84 (1%)
Query: 63 HPVLKNHTIQTWHKSGRCPKGTVPIRRIQKQDLLRAATLDRFGLKQSSSFVNSKNTTISN 122
HP K +T+H G CP G + D RA + + S S+ ++ N
Sbjct: 172 HPKYKTEYCRTFHSVGFCPYGP-RCHFVHNADEARAQQAAQAAKSSTQSQSQSQQSSSQN 230
Query: 123 FSKLSGSSNVVSEDHSGVHLATSG 146
FS S S+ S + S ++ G
Sbjct: 231 FSPKSNQSSNQSSNSSSSSSSSGG 254
>ER12_ARATH (O65402) Squalene monooxygenase 1,2 (EC 1.14.99.7)
(Squalene epoxidase 1,2) (SE 1,2)
Length = 517
Score = 31.2 bits (69), Expect = 4.6
Identities = 50/234 (21%), Positives = 95/234 (40%), Gaps = 24/234 (10%)
Query: 15 FVAGAISLSETEIEAKLKLH---NRPAVKTIKSENGDIIDCVDIYKQPAFDHPVLKNHTI 71
++ G IS +E KLH ++P+ + + + C ++ + P + N +
Sbjct: 227 YIVGYIS-KNCRLEEPEKLHLILSKPSFTMVYQISSTDVRCG--FEVLPENFPSIANGEM 283
Query: 72 QTWHKSGRCPKGTVPIRRIQKQDLLRAATLDRFGLKQSSSFVNSKNTTISNFSKLSGSSN 131
T+ K+ P+ +R+I + + A + K+ +S ++ K I +
Sbjct: 284 STFMKNTIVPQVPPKLRKIFLKGIDEGAHIKVVPAKRMTSTLSKKKGVIVLGDAFNMRHP 343
Query: 132 VVSEDHSGVHLATSGSNFIGAEADINVWNPKVDLPDDSTTAQIWLKAGNGNEFESIEAGW 191
VV+ SG+ + S + + P +L D + +++ N F I
Sbjct: 344 VVA---SGMMVLLSDILILR-----RLLQPLSNLGDANKVSEVI------NSFYDIRKPM 389
Query: 192 MVNPGLYGNHDTRLFSYWTTDSYKST---GCFDLTCSGFVQTSNTVALGGGINP 242
GN +++ +TD K G +D CSG +TS +AL GG+NP
Sbjct: 390 SATVNTLGNAFSQVL-IGSTDEAKEAMRQGVYDYLCSGGFRTSGMMALLGGMNP 442
>ER11_ARATH (O65404) Squalene monooxygenase 1,1 (EC 1.14.99.7)
(Squalene epoxidase 1,1) (SE 1,1)
Length = 516
Score = 30.8 bits (68), Expect = 6.0
Identities = 16/36 (44%), Positives = 21/36 (57%), Gaps = 3/36 (8%)
Query: 210 TTDSYKST---GCFDLTCSGFVQTSNTVALGGGINP 242
+TD K GC+D SG +TS +AL GG+NP
Sbjct: 406 STDEAKEAMRQGCYDYLSSGGFRTSGMMALLGGMNP 441
>SECY_PAVLU (P28540) Preprotein translocase secY subunit
Length = 419
Score = 30.4 bits (67), Expect = 7.8
Identities = 16/32 (50%), Positives = 21/32 (65%), Gaps = 2/32 (6%)
Query: 105 GLKQSSSFVNS--KNTTISNFSKLSGSSNVVS 134
G+ + SF S +NT+I N S LSG SNV+S
Sbjct: 35 GITEVESFYESSFRNTSIYNLSALSGGSNVIS 66
>NPR1_YEAST (P22211) Nitrogen permease reactivator protein (EC
2.7.1.-)
Length = 790
Score = 30.4 bits (67), Expect = 7.8
Identities = 20/65 (30%), Positives = 29/65 (43%), Gaps = 1/65 (1%)
Query: 86 PIRRIQKQDLLRAATLDRFGLKQSSSFVNSK-NTTISNFSKLSGSSNVVSEDHSGVHLAT 144
P + + + L RF K S+S ++S N I N S LS V S HS H A
Sbjct: 346 PSQSVPRSQHSSIGDLKRFFKKSSNSNLSSNSNNVIPNGSPLSSGIAVPSHSHSSSHFAA 405
Query: 145 SGSNF 149
+++
Sbjct: 406 GNNSY 410
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.315 0.132 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,519,319
Number of Sequences: 164201
Number of extensions: 1988312
Number of successful extensions: 4441
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 4434
Number of HSP's gapped (non-prelim): 12
length of query: 380
length of database: 59,974,054
effective HSP length: 112
effective length of query: 268
effective length of database: 41,583,542
effective search space: 11144389256
effective search space used: 11144389256
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 67 (30.4 bits)
Medicago: description of AC148485.6