BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148485.5 + phase: 0
(102 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1, m... 42 2e-04
YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergeni... 40 0.001
PIF1_YEAST (P07271) DNA repair and recombination protein PIF1, m... 40 0.001
YB68_SCHPO (Q09748) Dynamin-like protein C12C2.08 30 1.0
Y875_CHLTR (O84883) Protein CT875 30 1.0
ENO_MESCR (Q43130) Enolase (EC 4.2.1.11) (2-phosphoglycerate deh... 28 3.0
ENO_CUNEL (O74286) Enolase (EC 4.2.1.11) (2-phosphoglycerate deh... 28 3.0
ENO1_MAIZE (P26301) Enolase 1 (EC 4.2.1.11) (2-phosphoglycerate ... 28 3.0
ENO1_ENTHI (P51555) Enolase 1 (EC 4.2.1.11) (2-phosphoglycerate ... 28 3.0
DNM1_YEAST (P54861) Dynamin-related protein DNM1 (EC 3.6.5.5) 28 3.9
POLG_EC23C (Q9YID8) Genome polyprotein [Contains: Coat protein V... 28 5.1
ENO_ORYSA (Q42971) Enolase (EC 4.2.1.11) (2-phosphoglycerate deh... 28 5.1
RGA1_YEAST (P39083) Rho-type GTPase-activating protein 1 27 6.7
ENO_ALNGL (Q43321) Enolase (EC 4.2.1.11) (2-phosphoglycerate deh... 27 6.7
SYM_THEMA (O33925) Methionyl-tRNA synthetase (EC 6.1.1.10) (Meth... 27 8.7
RL14_RICPR (Q9ZCR5) 50S ribosomal protein L14 27 8.7
ENO2_MAIZE (P42895) Enolase 2 (EC 4.2.1.11) (2-phosphoglycerate ... 27 8.7
>PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1,
mitochondrial precursor
Length = 805
Score = 42.4 bits (98), Expect = 2e-04
Identities = 23/48 (47%), Positives = 32/48 (65%), Gaps = 1/48 (2%)
Query: 55 NVSITFERRQFPLVLSFAMTINKSQGQTLTSVGLYLPRPVFTHGQLYV 102
N + R Q PL+L++A++I+K+QGQTL V + L R VF GQ YV
Sbjct: 701 NGEVQASRSQIPLILAYAISIHKAQGQTLDRVKVDLGR-VFEKGQAYV 747
>YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergenic
region
Length = 723
Score = 40.0 bits (92), Expect = 0.001
Identities = 20/46 (43%), Positives = 33/46 (71%), Gaps = 1/46 (2%)
Query: 57 SITFERRQFPLVLSFAMTINKSQGQTLTSVGLYLPRPVFTHGQLYV 102
++ ER Q PL+L +A++I+K+QGQT+ + + L R +F GQ+YV
Sbjct: 641 NVGLERTQIPLMLCWALSIHKAQGQTIQRLKVDL-RRIFEAGQVYV 685
>PIF1_YEAST (P07271) DNA repair and recombination protein PIF1,
mitochondrial precursor
Length = 857
Score = 39.7 bits (91), Expect = 0.001
Identities = 22/41 (53%), Positives = 30/41 (72%), Gaps = 1/41 (2%)
Query: 62 RRQFPLVLSFAMTINKSQGQTLTSVGLYLPRPVFTHGQLYV 102
R Q PL+L+++++I+KSQGQTL V + L R VF GQ YV
Sbjct: 691 RVQLPLMLAWSLSIHKSQGQTLPKVKVDL-RRVFEKGQAYV 730
>YB68_SCHPO (Q09748) Dynamin-like protein C12C2.08
Length = 781
Score = 30.0 bits (66), Expect = 1.0
Identities = 17/62 (27%), Positives = 27/62 (43%), Gaps = 4/62 (6%)
Query: 44 IPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSV----GLYLPRPVFTHGQ 99
+ +++L+ G N R +PL L F T+N+SQ ++ L R F H
Sbjct: 238 LTKLDLMDQGTNAMDILSGRVYPLKLGFVATVNRSQSDIVSHKSMRDALQSERSFFEHHP 297
Query: 100 LY 101
Y
Sbjct: 298 AY 299
>Y875_CHLTR (O84883) Protein CT875
Length = 591
Score = 30.0 bits (66), Expect = 1.0
Identities = 23/66 (34%), Positives = 31/66 (46%), Gaps = 16/66 (24%)
Query: 13 CNGTRLIIVSLGKNV--ICARVIGG-AHAGEVV-------------YIPRMNLIPSGANV 56
CN S G++V +CARV G A AGE + Y PR +L P GA++
Sbjct: 358 CNSIEFSFGSFGEHVRVLCARVSRGLAAAGEAIRRCFSCCKGSTHRYAPRDDLSPEGASL 417
Query: 57 SITFER 62
+ T R
Sbjct: 418 AETLAR 423
>ENO_MESCR (Q43130) Enolase (EC 4.2.1.11) (2-phosphoglycerate
dehydratase) (2-phospho-D-glycerate hydro-lyase)
Length = 444
Score = 28.5 bits (62), Expect = 3.0
Identities = 15/54 (27%), Positives = 26/54 (47%)
Query: 34 GGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSVG 87
GG+HAG + + ++P+GA+ + + + I K GQ T+VG
Sbjct: 160 GGSHAGNKLAMQEFMILPTGASSFKEAMKMGSEVYHNLKSVIKKKYGQDATNVG 213
>ENO_CUNEL (O74286) Enolase (EC 4.2.1.11) (2-phosphoglycerate
dehydratase) (2-phospho-D-glycerate hydro-lyase)
(Fragment)
Length = 436
Score = 28.5 bits (62), Expect = 3.0
Identities = 15/54 (27%), Positives = 26/54 (47%)
Query: 34 GGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSVG 87
GG+HAG + + ++P+GA + + + IN+ GQ T+VG
Sbjct: 156 GGSHAGNKLAMQEFMIMPTGAKSFSEAMKLGSEVYHTLKKVINEKYGQDATNVG 209
>ENO1_MAIZE (P26301) Enolase 1 (EC 4.2.1.11) (2-phosphoglycerate
dehydratase 1) (2-phospho-D-glycerate hydro-lyase 1)
Length = 446
Score = 28.5 bits (62), Expect = 3.0
Identities = 15/54 (27%), Positives = 26/54 (47%)
Query: 34 GGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSVG 87
GG+HAG + + ++P+GA+ + + + I K GQ T+VG
Sbjct: 161 GGSHAGNKLAMQEFMILPTGASSFKEAMKMGVEVYHNLKSIIKKKYGQDATNVG 214
>ENO1_ENTHI (P51555) Enolase 1 (EC 4.2.1.11) (2-phosphoglycerate
dehydratase) (2-phospho-D-glycerate hydro-lyase)
Length = 436
Score = 28.5 bits (62), Expect = 3.0
Identities = 20/62 (32%), Positives = 26/62 (41%), Gaps = 1/62 (1%)
Query: 27 VICARVI-GGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTS 85
V C VI GGAHAG + + + P+GA R + I GQ T+
Sbjct: 145 VPCFNVINGGAHAGNALAMQEFMICPTGATNFHEALRMAAETYQCLKVVIKAKYGQDATN 204
Query: 86 VG 87
VG
Sbjct: 205 VG 206
>DNM1_YEAST (P54861) Dynamin-related protein DNM1 (EC 3.6.5.5)
Length = 757
Score = 28.1 bits (61), Expect = 3.9
Identities = 12/36 (33%), Positives = 20/36 (55%)
Query: 44 IPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQ 79
I +++L+ SG N + +PL L F +N+SQ
Sbjct: 243 ITKLDLMDSGTNALDILSGKMYPLKLGFVGVVNRSQ 278
>POLG_EC23C (Q9YID8) Genome polyprotein [Contains: Coat protein VP0
(P1AB); Coat protein VP3 (P1C); Coat protein VP1 (P1D);
Picornain 2A (EC 3.4.22.29) (Core protein P2A); Core
protein P2B; Core protein P2C; Core protein P3A;
Genome-linked protein VPG (P3
Length = 2188
Score = 27.7 bits (60), Expect = 5.1
Identities = 11/28 (39%), Positives = 18/28 (64%)
Query: 71 FAMTINKSQGQTLTSVGLYLPRPVFTHG 98
F + IN +QG ++ +Y P+P+ THG
Sbjct: 170 FQVQINVNQGTAGCALVVYEPKPIVTHG 197
>ENO_ORYSA (Q42971) Enolase (EC 4.2.1.11) (2-phosphoglycerate
dehydratase) (2-phospho-D-glycerate hydro-lyase) (OSE1)
Length = 446
Score = 27.7 bits (60), Expect = 5.1
Identities = 15/54 (27%), Positives = 25/54 (45%)
Query: 34 GGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSVG 87
GG+HAG + + ++P+GA + + + I K GQ T+VG
Sbjct: 161 GGSHAGNKLAMQEFMILPTGAASFKEAMKMGVEVYHNLKSVIKKKYGQDATNVG 214
>RGA1_YEAST (P39083) Rho-type GTPase-activating protein 1
Length = 1007
Score = 27.3 bits (59), Expect = 6.7
Identities = 12/47 (25%), Positives = 22/47 (46%)
Query: 43 YIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSVGLY 89
Y+ NL S +E + P++LS + +S + + S G+Y
Sbjct: 782 YLDGSNLYGSSLVARCNYENNEIPMILSVCIDFIESDEENMRSEGIY 828
>ENO_ALNGL (Q43321) Enolase (EC 4.2.1.11) (2-phosphoglycerate
dehydratase) (2-phospho-D-glycerate hydro-lyase)
Length = 440
Score = 27.3 bits (59), Expect = 6.7
Identities = 20/78 (25%), Positives = 33/78 (41%), Gaps = 4/78 (5%)
Query: 10 SGLCNGTRLIIVSLGKNVICARVIGGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVL 69
+ L +L++ NVI GG+HAG + + ++P GA+ + +
Sbjct: 141 ANLAGNPKLVLPVPAFNVIN----GGSHAGNKLAMQEFMILPVGASSFKEAMKMGVEVYH 196
Query: 70 SFAMTINKSQGQTLTSVG 87
I K GQ T+VG
Sbjct: 197 HLKAVIKKKYGQDATNVG 214
>SYM_THEMA (O33925) Methionyl-tRNA synthetase (EC 6.1.1.10)
(Methionine--tRNA ligase) (MetRS)
Length = 629
Score = 26.9 bits (58), Expect = 8.7
Identities = 13/20 (65%), Positives = 14/20 (70%)
Query: 83 LTSVGLYLPRPVFTHGQLYV 102
L SVGL LP+ VF HG L V
Sbjct: 274 LMSVGLPLPKKVFAHGWLTV 293
>RL14_RICPR (Q9ZCR5) 50S ribosomal protein L14
Length = 122
Score = 26.9 bits (58), Expect = 8.7
Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 5/34 (14%)
Query: 25 KNVICARVIGGAH-----AGEVVYIPRMNLIPSG 53
K V+C +V+GG+H G+V+ + IP G
Sbjct: 17 KKVMCIKVLGGSHHMMAKLGDVIVVSIKEAIPGG 50
>ENO2_MAIZE (P42895) Enolase 2 (EC 4.2.1.11) (2-phosphoglycerate
dehydratase 2) (2-phospho-D-glycerate hydro-lyase 2)
Length = 446
Score = 26.9 bits (58), Expect = 8.7
Identities = 15/54 (27%), Positives = 24/54 (43%)
Query: 34 GGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSVG 87
GG+HAG + + ++P+GA + + I K GQ T+VG
Sbjct: 161 GGSHAGNKLAMQEFMILPTGAASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVG 214
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.325 0.141 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,120,427
Number of Sequences: 164201
Number of extensions: 391206
Number of successful extensions: 1246
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 1238
Number of HSP's gapped (non-prelim): 17
length of query: 102
length of database: 59,974,054
effective HSP length: 78
effective length of query: 24
effective length of database: 47,166,376
effective search space: 1131993024
effective search space used: 1131993024
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148485.5