BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148483.13 + phase: 0
(63 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ARG3_MOUSE (Q9D8S3) ADP-ribosylation factor GTPase-activating pr... 91 6e-19
ARG3_HUMAN (Q9NP61) ADP-ribosylation factor GTPase-activating pr... 91 6e-19
YQP4_CAEEL (Q09531) Hypothetical protein F07F6.4 in chromosome III 89 2e-18
YDBH_SCHPO (Q10367) Hypothetical protein C22E12.17c in chromosome I 85 4e-17
GLO3_YEAST (P38682) ADP-ribosylation factor GTPase-activating pr... 81 4e-16
ARG1_RAT (Q62848) ADP-ribosylation factor GTPase activating prot... 75 4e-14
ARG1_MOUSE (Q9EPJ9) ADP-ribosylation factor GTPase activating pr... 75 4e-14
ARG1_HUMAN (Q8N6T3) ADP-ribosylation factor GTPase activating pr... 75 4e-14
GCS1_YEAST (P35197) ADP-ribosylation factor GTPase-activating pr... 68 5e-12
CEG1_HUMAN (Q99490) Centaurin gamma 1 64 1e-10
CEB5_HUMAN (Q96P50) Centaurin beta 5 (Cnt-b5) 64 1e-10
CEB2_HUMAN (Q15057) Centaurin beta 2 (Cnt-b2) 62 3e-10
CG1A_DROME (Q9NGC3) Centaurin gamma 1A protein 61 5e-10
CEG3_HUMAN (Q96P47) Centaurin gamma 3 61 6e-10
CEG2_MOUSE (Q8BXK8) Centaurin gamma 2 (ARF-GAP with GTP-binding ... 60 8e-10
CEG2_HUMAN (Q9UPQ3) Centaurin gamma 2 (ARF-GAP with GTP-binding ... 60 1e-09
CEB1_HUMAN (Q15027) Centaurin beta 1 (Cnt-b1) 59 2e-09
GIT2_MOUSE (Q9JLQ2) ARF GTPase-activating protein GIT2 (G protei... 57 7e-09
GIT2_HUMAN (Q14161) ARF GTPase-activating protein GIT2 (G protei... 57 1e-08
DDF1_MOUSE (Q9QWY8) 130-kDa phosphatidylinositol 4,5-biphosphate... 57 1e-08
>ARG3_MOUSE (Q9D8S3) ADP-ribosylation factor GTPase-activating
protein 3 (ARF GAP 3)
Length = 525
Score = 90.9 bits (224), Expect = 6e-19
Identities = 38/63 (60%), Positives = 49/63 (77%)
Query: 1 MASDSFTDKNAVFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHIS 60
M S D A+F++L++ NK CFDC AKNP+WAS++YG+FLCIDCS HRSLGVH+S
Sbjct: 1 MGDPSKQDILAIFKRLRSVPTNKVCFDCGAKNPSWASISYGVFLCIDCSGSHRSLGVHLS 60
Query: 61 FVR 63
F+R
Sbjct: 61 FIR 63
>ARG3_HUMAN (Q9NP61) ADP-ribosylation factor GTPase-activating
protein 3 (ARF GAP 3)
Length = 516
Score = 90.9 bits (224), Expect = 6e-19
Identities = 38/63 (60%), Positives = 48/63 (75%)
Query: 1 MASDSFTDKNAVFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHIS 60
M S D +F++L++ NK CFDC AKNP+WAS+TYG+FLCIDCS HRSLGVH+S
Sbjct: 1 MGDPSKQDILTIFKRLRSVPTNKVCFDCGAKNPSWASITYGVFLCIDCSGSHRSLGVHLS 60
Query: 61 FVR 63
F+R
Sbjct: 61 FIR 63
>YQP4_CAEEL (Q09531) Hypothetical protein F07F6.4 in chromosome
III
Length = 529
Score = 89.0 bits (219), Expect = 2e-18
Identities = 37/59 (62%), Positives = 45/59 (75%)
Query: 5 SFTDKNAVFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
S D RK++ NK CFDC A+NPTW +VTYG+FLCIDCSAVHR+LGVH++FVR
Sbjct: 8 SKVDLQTAMRKMRALPPNKLCFDCGARNPTWCTVTYGVFLCIDCSAVHRNLGVHLTFVR 66
>YDBH_SCHPO (Q10367) Hypothetical protein C22E12.17c in chromosome
I
Length = 486
Score = 84.7 bits (208), Expect = 4e-17
Identities = 33/56 (58%), Positives = 45/56 (79%)
Query: 8 DKNAVFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
+ + L+++ +NK CFDC AKNPTW+S T+GI+LC+DCSA HR++GVHISFVR
Sbjct: 7 ESQKLLTSLRSQRDNKVCFDCGAKNPTWSSTTFGIYLCLDCSAAHRNMGVHISFVR 62
>GLO3_YEAST (P38682) ADP-ribosylation factor GTPase-activating
protein GLO3
Length = 493
Score = 81.3 bits (199), Expect = 4e-16
Identities = 33/52 (63%), Positives = 42/52 (80%)
Query: 12 VFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
VF+KL + EN+ CFDC KNPTW SV +G+ LCI CSAVHR++GVHI+FV+
Sbjct: 18 VFQKLGSNMENRVCFDCGNKNPTWTSVPFGVMLCIQCSAVHRNMGVHITFVK 69
>ARG1_RAT (Q62848) ADP-ribosylation factor GTPase activating
protein 1 (ADP-ribosylation factor 1 GTPase activating
protein) (ARF1 GAP) (ARF1-directed GTPase-activating
protein) (GAP protein)
Length = 415
Score = 74.7 bits (182), Expect = 4e-14
Identities = 29/52 (55%), Positives = 40/52 (76%)
Query: 12 VFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
V ++++ + EN CF+C A NP W SVTYGI++C++CS HR LGVH+SFVR
Sbjct: 9 VLKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVR 60
>ARG1_MOUSE (Q9EPJ9) ADP-ribosylation factor GTPase activating
protein 1 (ADP-ribosylation factor 1 GTPase activating
protein) (ARF1 GAP) (ARF1-directed GTPase-activating
protein) (GAP protein)
Length = 414
Score = 74.7 bits (182), Expect = 4e-14
Identities = 29/52 (55%), Positives = 40/52 (76%)
Query: 12 VFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
V ++++ + EN CF+C A NP W SVTYGI++C++CS HR LGVH+SFVR
Sbjct: 9 VLKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVR 60
>ARG1_HUMAN (Q8N6T3) ADP-ribosylation factor GTPase activating
protein 1 (ADP-ribosylation factor 1 GTPase activating
protein) (ARF1 GAP) (ARF1-directed GTPase-activating
protein) (GAP protein)
Length = 406
Score = 74.7 bits (182), Expect = 4e-14
Identities = 29/52 (55%), Positives = 40/52 (76%)
Query: 12 VFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
V ++++ + EN CF+C A NP W SVTYGI++C++CS HR LGVH+SFVR
Sbjct: 9 VLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVR 60
>GCS1_YEAST (P35197) ADP-ribosylation factor GTPase-activating
protein GCS1
Length = 352
Score = 67.8 bits (164), Expect = 5e-12
Identities = 26/49 (53%), Positives = 35/49 (71%)
Query: 15 KLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
+L+ NK C DC A NP WA+ +G F+C++C+ +HR LGVHISFVR
Sbjct: 16 QLQKIGANKKCMDCGAPNPQWATPKFGAFICLECAGIHRGLGVHISFVR 64
>CEG1_HUMAN (Q99490) Centaurin gamma 1
Length = 836
Score = 63.5 bits (153), Expect = 1e-10
Identities = 27/63 (42%), Positives = 41/63 (64%), Gaps = 1/63 (1%)
Query: 1 MASDSFTDKNAVFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHIS 60
+ +DS ++ A+ + ++ N C DC A NPTWAS+ G +CI+CS +HR+LG H+S
Sbjct: 567 LRTDSQSEAVAI-QAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLS 625
Query: 61 FVR 63
VR
Sbjct: 626 RVR 628
>CEB5_HUMAN (Q96P50) Centaurin beta 5 (Cnt-b5)
Length = 759
Score = 63.5 bits (153), Expect = 1e-10
Identities = 25/53 (47%), Positives = 37/53 (69%)
Query: 11 AVFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
+V +++++ + N C DC +P WAS+ G+ LCI+CS +HRSLGVH S VR
Sbjct: 362 SVLQRVQSVAGNSQCGDCGQPDPRWASINLGVLLCIECSGIHRSLGVHCSKVR 414
>CEB2_HUMAN (Q15057) Centaurin beta 2 (Cnt-b2)
Length = 778
Score = 62.0 bits (149), Expect = 3e-10
Identities = 26/42 (61%), Positives = 30/42 (70%)
Query: 22 NKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
N SC DC +P WAS+ GI LCI+CS +HRSLGVH S VR
Sbjct: 411 NASCCDCGLADPRWASINLGITLCIECSGIHRSLGVHFSKVR 452
>CG1A_DROME (Q9NGC3) Centaurin gamma 1A protein
Length = 995
Score = 61.2 bits (147), Expect = 5e-10
Identities = 28/57 (49%), Positives = 35/57 (61%)
Query: 7 TDKNAVFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
TD A+ + N C DC A NP WAS+ G+ +CI+CS VHR+LG HIS VR
Sbjct: 699 TDLAAMLAIRQRVPGNGFCVDCGAPNPEWASLNLGVLMCIECSGVHRNLGSHISKVR 755
>CEG3_HUMAN (Q96P47) Centaurin gamma 3
Length = 876
Score = 60.8 bits (146), Expect = 6e-10
Identities = 24/48 (50%), Positives = 32/48 (66%)
Query: 16 LKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
++T N C DC+A NP WAS+ G +CI+CS +HR LG H+S VR
Sbjct: 633 VRTVRGNSFCIDCDAPNPDWASLNLGALMCIECSGIHRHLGAHLSRVR 680
>CEG2_MOUSE (Q8BXK8) Centaurin gamma 2 (ARF-GAP with GTP-binding
protein-like, ankyrin repeat and pleckstrin homology
domains 1) (AGAP1)
Length = 857
Score = 60.5 bits (145), Expect = 8e-10
Identities = 22/50 (44%), Positives = 33/50 (66%)
Query: 14 RKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
+ ++ N C DC+ +NP WAS+ G +CI+CS +HR+LG H+S VR
Sbjct: 613 QSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVR 662
>CEG2_HUMAN (Q9UPQ3) Centaurin gamma 2 (ARF-GAP with GTP-binding
protein-like, ankyrin repeat and pleckstrin homology
domains 1) (AGAP1)
Length = 857
Score = 60.1 bits (144), Expect = 1e-09
Identities = 22/50 (44%), Positives = 32/50 (64%)
Query: 14 RKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
+ ++ N C DC +NP WAS+ G +CI+CS +HR+LG H+S VR
Sbjct: 613 QSIRNMRGNSHCVDCETQNPNWASLNLGALMCIECSGIHRNLGTHLSRVR 662
>CEB1_HUMAN (Q15027) Centaurin beta 1 (Cnt-b1)
Length = 740
Score = 58.9 bits (141), Expect = 2e-09
Identities = 25/52 (48%), Positives = 32/52 (61%)
Query: 12 VFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
V ++++ N C DC P WAS+ G+ LCI CS +HRSLGVH S VR
Sbjct: 407 VVAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVR 458
>GIT2_MOUSE (Q9JLQ2) ARF GTPase-activating protein GIT2 (G
protein-coupled receptor kinase-interactor 2)
(Cool-interacting tyrosine-phosphorylated protein 2)
(CAT2) (CAT-2)
Length = 708
Score = 57.4 bits (137), Expect = 7e-09
Identities = 24/39 (61%), Positives = 29/39 (73%)
Query: 25 CFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
C DCN +P+WASV G F+C +C +VHRSLG HIS VR
Sbjct: 11 CADCNGPDPSWASVNRGTFICDECCSVHRSLGRHISQVR 49
>GIT2_HUMAN (Q14161) ARF GTPase-activating protein GIT2 (G
protein-coupled receptor kinase-interactor 2)
(GRK-interacting protein 2) (Cool-interacting
tyrosine-phosphorylated protein 2) (CAT2) (CAT-2)
Length = 759
Score = 56.6 bits (135), Expect = 1e-08
Identities = 25/47 (53%), Positives = 32/47 (67%)
Query: 17 KTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
K ++ C DC+ +P+WASV G FLC +C +VHRSLG HIS VR
Sbjct: 3 KRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVR 49
>DDF1_MOUSE (Q9QWY8) 130-kDa phosphatidylinositol
4,5-biphosphate-dependent ARF1 GTPase-activating protein
(PIP2-dependent ARF1 GAP) (ADP-ribosylation
factor-directed GTPase-activating protein 1) (ARF
GTPase-activating protein 1) (Development and differen
Length = 1147
Score = 56.6 bits (135), Expect = 1e-08
Identities = 22/53 (41%), Positives = 31/53 (57%)
Query: 11 AVFRKLKTKSENKSCFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVR 63
A+ ++ N C DC + PTW S GI CI+CS +HR +GVHIS ++
Sbjct: 455 AIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQ 507
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.324 0.132 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,875,782
Number of Sequences: 164201
Number of extensions: 196069
Number of successful extensions: 529
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 39
Number of HSP's successfully gapped in prelim test: 25
Number of HSP's that attempted gapping in prelim test: 487
Number of HSP's gapped (non-prelim): 66
length of query: 63
length of database: 59,974,054
effective HSP length: 39
effective length of query: 24
effective length of database: 53,570,215
effective search space: 1285685160
effective search space used: 1285685160
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148483.13