BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148482.6 - phase: 0
(402 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AIRC_ARATH (Q94BT2) Auxin-induced in root cultures protein 12 pr... 97 6e-20
YIQ9_YEAST (P40442) Hypothetical 99.7 kDa protein in SDL1 5'regi... 33 1.7
CYB_VENIN (O48334) Cytochrome b 32 2.9
AMT4_PSEST (P13507) Glucan 1,4-alpha-maltotetraohydrolase precur... 31 4.9
VSI1_REOVD (P03528) Sigma 1 protein precursor (Hemagglutinin) (C... 30 8.4
VP12_RDVF (Q85443) Nonstructural protein Pns12A 30 8.4
LY_BPSF6 (P21270) Lysozyme (EC 3.2.1.17) 30 8.4
GDF1_MOUSE (P20863) Embryonic growth/differentiation factor 1 pr... 30 8.4
DHSB_CHOCR (P48932) Succinate dehydrogenase [ubiquinone] iron-su... 30 8.4
>AIRC_ARATH (Q94BT2) Auxin-induced in root cultures protein 12
precursor
Length = 252
Score = 97.4 bits (241), Expect = 6e-20
Identities = 68/252 (26%), Positives = 110/252 (42%), Gaps = 25/252 (9%)
Query: 1 MVKNLRFMFLISILMFVTTTLAQTTTQTCKSQNFTNNAIFTSCRDLPQLTSYLHWTYDQT 60
M + + ++++ FV+ + Q CKSQN + F SC DLP L SYLH+TY+ +
Sbjct: 1 MASSSSSLLILAVACFVSLISPAISQQACKSQNLNSAGPFDSCEDLPVLNSYLHYTYNSS 60
Query: 61 TGKLDIAFRHKGITDTNRWVAWAINPNNDLASSMNGAQALVAILQSSGTPKAYTSSIANS 120
L +AF WVAWAINP + M G+QA +A G + +S
Sbjct: 61 NSSLSVAFVATPSQANGGWVAWAINPT---GTKMAGSQAFLAYRSGGGAAPVVKTYNISS 117
Query: 121 RTQLAESNISYPHSGLIA-THENNEVTIYASITLPVGTPSLVHLWQ-DGAMSGSTPQMHD 178
+ L E +++ L A + + I+ ++ +P G S+ +WQ G ++ P +H
Sbjct: 118 YSSLVEGKLAFDFWNLRAESLSGGRIAIFTTVKVPAGADSVNQVWQIGGNVTNGRPGVHP 177
Query: 179 MTSANTQSKESLDL-------------------RSGASEQG-SGGGSLSRRRNTHGVLNA 218
N S L SG++ G + GG + T V
Sbjct: 178 FGPDNLGSHRVLSFTEDAAPGSAPSPGSAPAPGTSGSTTPGTAAGGPGNAGSLTRNVNFG 237
Query: 219 ISWGILMPLGAV 230
++ GIL+ LG++
Sbjct: 238 VNLGILVLLGSI 249
>YIQ9_YEAST (P40442) Hypothetical 99.7 kDa protein in SDL1 5'region
precursor
Length = 995
Score = 32.7 bits (73), Expect = 1.7
Identities = 31/141 (21%), Positives = 69/141 (47%), Gaps = 16/141 (11%)
Query: 73 ITDTNRWVAWAINPNNDLASSMNGAQALVA-----ILQSSGTPKAYTSSIANSRTQLAES 127
++D + V+ + + +D++SS++ + + + + QSS + +SS++ S + ++
Sbjct: 102 VSDVSSSVSQSSSSASDVSSSVSQSASSTSDVSSSVSQSSSSASDVSSSVSQSSSSASDV 161
Query: 128 NISYPHSGLIATHENNEVTIYASITLPVGTPSLVHLWQDGAMSGSTPQMHDMTSANTQSK 187
+ S S A+ ++ V+ AS T V + ++S S+ D++S+ +QS
Sbjct: 162 SSSVSQSASSASDVSSSVSQSASSTSDVSS----------SVSQSSSSASDVSSSVSQSS 211
Query: 188 ESL-DLRSGASEQGSGGGSLS 207
S D+ S S+ S +S
Sbjct: 212 SSASDVSSSVSQSASSTSDVS 232
Score = 31.6 bits (70), Expect = 3.8
Identities = 41/211 (19%), Positives = 94/211 (44%), Gaps = 22/211 (10%)
Query: 6 RFMFLISILMFVTTTLAQT-TTQTCKSQNFTNNAIFTSCRDLPQLTSYLHWTYDQTTGKL 64
+F +++ ++ + L Q + T S N ++ ++ +S ++S + T T
Sbjct: 7 KFQAALALALYSQSALGQYYSNSTSISSNSSSTSVVSSSSGSVSISSSIAETSSSAT--- 63
Query: 65 DIAFRHKGITDTNRWVAWAINPNND--LASSMNGAQALVA-----ILQSSGTPKAYTSSI 117
DI + V+ ++ P++ ++SS++ + + V+ + QSS + +SS+
Sbjct: 64 DILSSITQSASSTSGVSSSVGPSSSSVVSSSVSQSSSSVSDVSSSVSQSSSSASDVSSSV 123
Query: 118 ANSRTQLAESNISYPHSGLIATHENNEVTIYASITLPVGTPSLVHLWQDGAMSGSTPQMH 177
+ S + ++ + S S A+ ++ V+ +S V + ++S S
Sbjct: 124 SQSASSTSDVSSSVSQSSSSASDVSSSVSQSSSSASDVSS----------SVSQSASSAS 173
Query: 178 DMTSANTQSKESL-DLRSGASEQGSGGGSLS 207
D++S+ +QS S D+ S S+ S +S
Sbjct: 174 DVSSSVSQSASSTSDVSSSVSQSSSSASDVS 204
Score = 31.2 bits (69), Expect = 4.9
Identities = 38/168 (22%), Positives = 70/168 (41%), Gaps = 9/168 (5%)
Query: 74 TDTNRWVAWAINPNNDLASSMNGAQALVA-----ILQSSGTPKAYTSSIANSRTQLAESN 128
+D + V+ + + +D++SS++ + + + + QSS + +SS++ S + ++ +
Sbjct: 159 SDVSSSVSQSASSASDVSSSVSQSASSTSDVSSSVSQSSSSASDVSSSVSQSSSSASDVS 218
Query: 129 ISYPHSGLIATHENNEVTIYASITLPVGTPSLVHLWQDGAMSGSTPQMHDMTSANTQSKE 188
S S + ++ V+ AS T V + + S S PQ S + S
Sbjct: 219 SSVSQSASSTSDVSSSVSQSASSTSGVSSSGSQSVSSASGSSSSFPQSTSSASTASGSAT 278
Query: 189 SLDL----RSGASEQGSGGGSLSRRRNTHGVLNAISWGILMPLGAVIA 232
S L S +S + SLS T + G L G VIA
Sbjct: 279 SNSLSSITSSASSASATASNSLSSSDGTIYLPTTTISGDLTLTGKVIA 326
>CYB_VENIN (O48334) Cytochrome b
Length = 393
Score = 32.0 bits (71), Expect = 2.9
Identities = 27/97 (27%), Positives = 39/97 (39%), Gaps = 12/97 (12%)
Query: 237 VFKSADPAWF--YLHVTCQSAAYIVGVAGWGTGLKLGSDSAGVTYSTHRTLGIVIFCLGT 294
+ + + W YLH SA + + G GL GS A T TLG++IF L
Sbjct: 68 IMRDVNNGWLIRYLHANTASAFFFIVYLHMGRGLYYGSYRAPRTLVW--TLGVIIFILMI 125
Query: 295 LQVFALLLRPKKDHKIRFYWNLYHWGVGYATIIISII 331
+ F + P Y + WG T ++S I
Sbjct: 126 VTAFLGYVLP--------YGQMSLWGATVITNLMSAI 154
>AMT4_PSEST (P13507) Glucan 1,4-alpha-maltotetraohydrolase precursor
(EC 3.2.1.60) (G4-amylase) (Maltotetraose-forming
amylase) (Exo-maltotetraohydrolase)
(Maltotetraose-forming exo-amylase)
Length = 548
Score = 31.2 bits (69), Expect = 4.9
Identities = 35/124 (28%), Positives = 51/124 (40%), Gaps = 32/124 (25%)
Query: 98 QALVAILQSSGTPKAYTS---------------SIANSRTQLAESNISYP--HSGLIATH 140
QA IL S GTP Y S + + A+S IS+ +SGL+AT
Sbjct: 338 QAYAYILTSPGTPVVYWSHMYDWGYGDFIRQLIQVRRAAGVRADSAISFHSGYSGLVATV 397
Query: 141 ENNEVTIYASITLPVGTPSLVHLWQDGAMSGSTPQMHDMTSANTQSKESLDLRSGASEQG 200
++ T+ ++ +G P V SGS + + N + + RSG G
Sbjct: 398 SGSQQTLVVALNSDLGNPGQV-------ASGSFSE-----AVNASNGQVRVWRSGT---G 442
Query: 201 SGGG 204
SGGG
Sbjct: 443 SGGG 446
>VSI1_REOVD (P03528) Sigma 1 protein precursor (Hemagglutinin) (Cell
attachment protein) (Minor outer capsid protein)
Length = 455
Score = 30.4 bits (67), Expect = 8.4
Identities = 25/100 (25%), Positives = 48/100 (48%), Gaps = 11/100 (11%)
Query: 71 KGITDTNRWVAWAINPNNDLASSMNGAQALVAILQSS-GTPKAYTSSIANSRTQLAE--- 126
+G+ D N+ + +DL +S++ AQ ++ L+SS G + + + +S TQL
Sbjct: 51 QGLDDANKRIIALEQSRDDLVASVSDAQLAISRLESSIGALQTVVNGLDSSVTQLGARVG 110
Query: 127 ------SNISYPHSGLIATHENNEVTIYASITLPVGTPSL 160
+++ H L+A + E I S+T + T +L
Sbjct: 111 QLETGLADVRVDHDNLVARVDTAERNI-GSLTTELSTLTL 149
>VP12_RDVF (Q85443) Nonstructural protein Pns12A
Length = 312
Score = 30.4 bits (67), Expect = 8.4
Identities = 15/49 (30%), Positives = 24/49 (48%)
Query: 171 GSTPQMHDMTSANTQSKESLDLRSGASEQGSGGGSLSRRRNTHGVLNAI 219
G P D T S+ S +++ SE+ +GG L+ R +HG + I
Sbjct: 115 GRGPSTSDARHTATDSRLSQEVKQPFSEENAGGNDLNTGRGSHGTGDGI 163
>LY_BPSF6 (P21270) Lysozyme (EC 3.2.1.17)
Length = 316
Score = 30.4 bits (67), Expect = 8.4
Identities = 25/100 (25%), Positives = 48/100 (48%), Gaps = 11/100 (11%)
Query: 71 KGITDTNRWVAWAINPNNDLASSMNGAQALVAILQSS-GTPKAYTSSIANSRTQLAE--- 126
+G+ D N+ + +DL +S++ AQ ++ L+SS G + + + +S TQL
Sbjct: 51 QGLDDANKRIIALEQSRDDLVASVSDAQLAISRLESSIGALQTVVNGLDSSVTQLGARVG 110
Query: 127 ------SNISYPHSGLIATHENNEVTIYASITLPVGTPSL 160
+++ H L+A + E I S+T + T +L
Sbjct: 111 QLETGLADVRVDHDNLVARVDTAERNI-GSLTTELSTLTL 149
>GDF1_MOUSE (P20863) Embryonic growth/differentiation factor 1
precursor (GDF-1)
Length = 357
Score = 30.4 bits (67), Expect = 8.4
Identities = 18/57 (31%), Positives = 25/57 (43%), Gaps = 6/57 (10%)
Query: 61 TGKLDIAFRHKGITDTNRWVAWAINPNNDLASSMNGAQALVAILQSSGTPKAYTSSI 117
T +L ++FR G W W I P LA+ G AL L+ G P A ++
Sbjct: 253 TRRLHVSFREVG------WHRWVIAPRGFLANFCQGTCALPETLRGPGGPPALNHAV 303
>DHSB_CHOCR (P48932) Succinate dehydrogenase [ubiquinone]
iron-sulfur protein (EC 1.3.5.1) (IP)
Length = 250
Score = 30.4 bits (67), Expect = 8.4
Identities = 12/28 (42%), Positives = 19/28 (67%)
Query: 364 GVAVLLEAYTWIIVIKRKKSENKLQGMN 391
G A+LL+AY WI+ + +EN+L +N
Sbjct: 185 GPAILLQAYRWIVDSRDNSTENRLNFLN 212
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.133 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,242,029
Number of Sequences: 164201
Number of extensions: 1910274
Number of successful extensions: 4859
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 4852
Number of HSP's gapped (non-prelim): 11
length of query: 402
length of database: 59,974,054
effective HSP length: 112
effective length of query: 290
effective length of database: 41,583,542
effective search space: 12059227180
effective search space used: 12059227180
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 67 (30.4 bits)
Medicago: description of AC148482.6