BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148404.4 - phase: 0
(385 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SOX3_XENLA (P55863) Transcription factor SOX-3 (SOX-11) 33 1.2
SPO1_RAT (P35446) Spondin 1 precursor (F-spondin) 32 2.1
SPO1_MOUSE (Q8VCC9) Spondin 1 precursor (F-spondin) 32 2.7
ATH6_ARATH (P46668) Homeobox-leucine zipper protein ATHB-6 (Home... 32 2.7
WWP1_MOUSE (Q8BZZ3) Nedd-4-like E3 ubiquitin-protein ligase WWP1... 32 3.6
SPO1_HUMAN (Q9HCB6) Spondin 1 precursor (F-spondin) (Vascular sm... 32 3.6
SPO1_BOVIN (Q9GLX9) Spondin 1 precursor (F-spondin) (Vascular sm... 32 3.6
R11D_LOTJA (Q40194) Ras-related protein Rab11D 32 3.6
WWP1_HUMAN (Q9H0M0) Nedd-4-like E3 ubiquitin-protein ligase WWP1... 31 4.6
SPO1_CHICK (Q9W770) Spondin 1 precursor (F-spondin) 31 4.6
R11E_LOTJA (Q40195) Ras-related protein Rab11E 31 4.6
GCN2_YEAST (P15442) Serine/threonine-protein kinase GCN2 (EC 2.7... 31 4.6
YM8D_YEAST (P38429) Hypothetical 23.0 kDa protein in TPS3-IPP2 i... 31 6.1
YHO0_YEAST (P38800) Hypothetical 149.7 kDa protein in IRE1-KSP1 ... 31 6.1
LPXB_RICCN (Q92II0) Lipid-A-disaccharide synthase (EC 2.4.1.182) 31 6.1
SPO1_XENLA (P35447) Spondin 1 precursor (F-spondin) 30 7.9
ITBX_DROME (P11584) Integrin beta-PS precursor (Position-specifi... 30 7.9
GCSH_SHEON (Q8EIQ7) Glycine cleavage system H protein 30 7.9
>SOX3_XENLA (P55863) Transcription factor SOX-3 (SOX-11)
Length = 309
Score = 33.1 bits (74), Expect = 1.2
Identities = 18/42 (42%), Positives = 21/42 (49%)
Query: 18 KTFSDNLKKKLPGNVTLKGPSGVVWNVGLTARDDTIYFTNGW 59
KT K LPGN+ G S V +VG+ R DT NGW
Sbjct: 116 KTLLKKDKYSLPGNLLAPGVSPVASSVGVGQRIDTYAHMNGW 157
>SPO1_RAT (P35446) Spondin 1 precursor (F-spondin)
Length = 807
Score = 32.3 bits (72), Expect = 2.1
Identities = 22/61 (36%), Positives = 34/61 (55%), Gaps = 3/61 (4%)
Query: 6 NWKSINLLQALPKTFSDNLKKKLPGNVTLKGPSGVVWNVGLTARDDTIYFTNGW-QRFVK 64
+W+ +N+ A FS + + L +T+ GPS WNVGL+A +D GW Q+ V+
Sbjct: 291 SWQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPS-PDWNVGLSA-EDLCTKECGWVQKVVQ 348
Query: 65 D 65
D
Sbjct: 349 D 349
>SPO1_MOUSE (Q8VCC9) Spondin 1 precursor (F-spondin)
Length = 807
Score = 32.0 bits (71), Expect = 2.7
Identities = 22/60 (36%), Positives = 33/60 (54%), Gaps = 3/60 (5%)
Query: 7 WKSINLLQALPKTFSDNLKKKLPGNVTLKGPSGVVWNVGLTARDDTIYFTNGW-QRFVKD 65
W+ +N+ A FS + + L +T+ GPS WNVGL+A +D GW Q+ V+D
Sbjct: 292 WQPVNVRAAPSAEFSVDRTRHLMSFLTMMGPS-PDWNVGLSA-EDLCTKECGWVQKVVQD 349
>ATH6_ARATH (P46668) Homeobox-leucine zipper protein ATHB-6
(Homeodomain transcription factor ATHB-6) (HD-ZIP
protein ATHB-6)
Length = 311
Score = 32.0 bits (71), Expect = 2.7
Identities = 39/137 (28%), Positives = 53/137 (38%), Gaps = 12/137 (8%)
Query: 169 QQLALPKTFSDNLNKKLPENVTLKGPGGVAWKIGLITRDDTVYFLNGWQRFVNDHSLKDN 228
Q AL K F L KL +K +A ++GL R V+F N R+ KD
Sbjct: 70 QVKALEKNFE--LENKLEPERKVK----LAQELGLQPRQVAVWFQNRRARWKTKQLEKDY 123
Query: 229 DFLVFNYNGESH-FDVLIFDGESFCEKEASYFVGKCSHTQTELGGSKANETNNSIEEVNT 287
L Y+ H FD L D ES ++ + K GG + E NN+ +
Sbjct: 124 GVLKTQYDSLRHNFDSLRRDNESLLQE-----ISKLKTKLNGGGGEEEEEENNAAVTTES 178
Query: 288 ASSDGGVECGLYEKFQD 304
S E L EK +
Sbjct: 179 DISVKEEEVSLPEKITE 195
>WWP1_MOUSE (Q8BZZ3) Nedd-4-like E3 ubiquitin-protein ligase WWP1
(EC 6.3.2.-) (WW domain-containing protein 1)
Length = 918
Score = 31.6 bits (70), Expect = 3.6
Identities = 30/145 (20%), Positives = 58/145 (39%), Gaps = 12/145 (8%)
Query: 122 NNSVEEVSTPSNG--SVECSLPEKSWEEDIYW--THFQFIHFTQLLPEDFKQQLALPKTF 177
N P NG SV P+ ++E W HF+++ + LP K ++ F
Sbjct: 514 NTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFRYLCQSNALPSHVKINVSRQTLF 573
Query: 178 SDNLNKKLPENVTLKGPGGVAWKIGLITRDDTVYFLNGWQR---FVNDHSLKDNDFLVFN 234
D+ + + P + ++ +I R + G R F+ H + + + +F
Sbjct: 574 EDSF-----QQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFE 628
Query: 235 YNGESHFDVLIFDGESFCEKEASYF 259
Y G++++ + I + SYF
Sbjct: 629 YAGKNNYCLQINPASTINPDHLSYF 653
>SPO1_HUMAN (Q9HCB6) Spondin 1 precursor (F-spondin) (Vascular
smooth muscle cell growth promoting factor)
Length = 807
Score = 31.6 bits (70), Expect = 3.6
Identities = 22/60 (36%), Positives = 33/60 (54%), Gaps = 3/60 (5%)
Query: 7 WKSINLLQALPKTFSDNLKKKLPGNVTLKGPSGVVWNVGLTARDDTIYFTNGW-QRFVKD 65
W+ +N+ A FS + + L +T+ GPS WNVGL+A +D GW Q+ V+D
Sbjct: 292 WQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPS-PDWNVGLSA-EDLCTKECGWVQKVVQD 349
>SPO1_BOVIN (Q9GLX9) Spondin 1 precursor (F-spondin) (Vascular
smooth muscle cell growth promoting factor)
Length = 807
Score = 31.6 bits (70), Expect = 3.6
Identities = 22/60 (36%), Positives = 33/60 (54%), Gaps = 3/60 (5%)
Query: 7 WKSINLLQALPKTFSDNLKKKLPGNVTLKGPSGVVWNVGLTARDDTIYFTNGW-QRFVKD 65
W+ +N+ A FS + + L +T+ GPS WNVGL+A +D GW Q+ V+D
Sbjct: 292 WQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPS-PDWNVGLSA-EDLCTKECGWVQKVVQD 349
>R11D_LOTJA (Q40194) Ras-related protein Rab11D
Length = 218
Score = 31.6 bits (70), Expect = 3.6
Identities = 31/118 (26%), Positives = 47/118 (39%), Gaps = 25/118 (21%)
Query: 204 ITRDDTVYFLNGWQRFVNDHSLKDNDFLVFNYNGESHFDVLIF----DGESFCEKEASYF 259
+TR T W + + DH+ D + +V +S L+ DG+SF E+E+ YF
Sbjct: 95 VTRRATFENAARWLKELRDHT--DPNIVVMLIGNKSDLRHLVAVPTEDGKSFAERESLYF 152
Query: 260 VGKCSHTQTELGGSKANETNNSIEEVNT-----------ASSDGGVECGLYEKFQDLN 306
+ E +A N+ EV T + D G GL K Q +N
Sbjct: 153 M--------ETSALEATNVENAFTEVLTQIYRIVSKRAVEAGDSGSSSGLPSKGQTIN 202
>WWP1_HUMAN (Q9H0M0) Nedd-4-like E3 ubiquitin-protein ligase WWP1
(EC 6.3.2.-) (WW domain-containing protein 1) (Atropin-1
interacting protein 5) (AIP5)
Length = 922
Score = 31.2 bits (69), Expect = 4.6
Identities = 30/145 (20%), Positives = 58/145 (39%), Gaps = 12/145 (8%)
Query: 122 NNSVEEVSTPSNG--SVECSLPEKSWEEDIYW--THFQFIHFTQLLPEDFKQQLALPKTF 177
N P NG SV P+ ++E W HF+++ + LP K ++ F
Sbjct: 518 NTRTTTFKDPRNGKSSVTKGGPQIAYERGFRWKLAHFRYLCQSNALPSHVKINVSRQTLF 577
Query: 178 SDNLNKKLPENVTLKGPGGVAWKIGLITRDDTVYFLNGWQR---FVNDHSLKDNDFLVFN 234
D+ + + P + ++ +I R + G R F+ H + + + +F
Sbjct: 578 EDSF-----QQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFE 632
Query: 235 YNGESHFDVLIFDGESFCEKEASYF 259
Y G++++ + I + SYF
Sbjct: 633 YAGKNNYCLQINPASTINPDHLSYF 657
>SPO1_CHICK (Q9W770) Spondin 1 precursor (F-spondin)
Length = 802
Score = 31.2 bits (69), Expect = 4.6
Identities = 22/60 (36%), Positives = 33/60 (54%), Gaps = 3/60 (5%)
Query: 7 WKSINLLQALPKTFSDNLKKKLPGNVTLKGPSGVVWNVGLTARDDTIYFTNGW-QRFVKD 65
W+ +N+ A FS + + L +T+ GPS WNVGL+A +D GW Q+ V+D
Sbjct: 287 WQPLNVRAAPSAEFSVDRHRHLMSFLTMLGPS-PDWNVGLSA-EDLCTKDCGWVQKVVQD 344
>R11E_LOTJA (Q40195) Ras-related protein Rab11E
Length = 218
Score = 31.2 bits (69), Expect = 4.6
Identities = 24/88 (27%), Positives = 38/88 (42%), Gaps = 14/88 (15%)
Query: 204 ITRDDTVYFLNGWQRFVNDHSLKDNDFLVFNYNGESHFDVLIF----DGESFCEKEASYF 259
+TR T W + + DH+ D + +V +S L+ DG++F EKE+ YF
Sbjct: 95 VTRSTTFETAGRWLKELRDHT--DPNIVVMLIGNKSDLRHLVTVSTEDGKAFAEKESLYF 152
Query: 260 VGKCSHTQTELGGSKANETNNSIEEVNT 287
+ E +A N+ EV T
Sbjct: 153 M--------ETSALEATNVENAFSEVLT 172
>GCN2_YEAST (P15442) Serine/threonine-protein kinase GCN2 (EC
2.7.1.37)
Length = 1590
Score = 31.2 bits (69), Expect = 4.6
Identities = 17/57 (29%), Positives = 26/57 (44%)
Query: 324 FNAGVESDSPELLIADTVTKTTAIQFPYQPTGKRSKRRRSSTSANCCWRALDRVAYN 380
F+A + S +IA T I F +P+GK+S R + N W + +A N
Sbjct: 1238 FHAVYDDGSSRNMIAAGGRYDTLISFFARPSGKKSSNTRKAVGFNLAWETIFGIAQN 1294
>YM8D_YEAST (P38429) Hypothetical 23.0 kDa protein in TPS3-IPP2
intergenic region (ORF2)
Length = 201
Score = 30.8 bits (68), Expect = 6.1
Identities = 20/82 (24%), Positives = 40/82 (48%), Gaps = 4/82 (4%)
Query: 116 NKGKSSNNSVEEVSTPSNGSVECSLPEKSWEEDIYWTHFQFIH---FTQLLPEDFKQQL- 171
N S+NN+ S +NG + ++ + +++ TH H + P DF++
Sbjct: 50 NSNNSNNNNGPTSSGRTNGKQRLTAAQQQYIKNLIETHITDNHPDLRPKSHPMDFEEYTD 109
Query: 172 ALPKTFSDNLNKKLPENVTLKG 193
A + + D+ +P+N+TL+G
Sbjct: 110 AFLRRYKDHFQLDVPDNLTLQG 131
>YHO0_YEAST (P38800) Hypothetical 149.7 kDa protein in IRE1-KSP1
intergenic region
Length = 1345
Score = 30.8 bits (68), Expect = 6.1
Identities = 29/110 (26%), Positives = 43/110 (38%), Gaps = 4/110 (3%)
Query: 261 GKCSHTQTELGGSKANETNNSIEEVNTASSDGGVECGLYEKFQDLNNIGTPLAVPVETTN 320
G HT E S A+ + I ++N +D G K + N+ A+ +TN
Sbjct: 162 GHDEHTAFETFLSFAHNAVSHIPKINVQDADNGTISRNEPKDRKKNSSNISGALSENSTN 221
Query: 321 EKTFNAGVESDSPELLIADTVTKTTAIQFP----YQPTGKRSKRRRSSTS 366
K ++ ESD P L D + + P T SK + SS S
Sbjct: 222 NKNTSSTKESDGPFLKNLDNILAASKSSTPSNQQLNTTEAGSKSKPSSLS 271
>LPXB_RICCN (Q92II0) Lipid-A-disaccharide synthase (EC 2.4.1.182)
Length = 446
Score = 30.8 bits (68), Expect = 6.1
Identities = 14/44 (31%), Positives = 22/44 (49%)
Query: 152 THFQFIHFTQLLPEDFKQQLALPKTFSDNLNKKLPENVTLKGPG 195
T + F ++LP FK + + KT D +N K +T+ PG
Sbjct: 67 TSINLMGFVEILPHIFKLKKLIDKTVEDIINSKADLLITIDSPG 110
>SPO1_XENLA (P35447) Spondin 1 precursor (F-spondin)
Length = 803
Score = 30.4 bits (67), Expect = 7.9
Identities = 21/60 (35%), Positives = 32/60 (53%), Gaps = 3/60 (5%)
Query: 7 WKSINLLQALPKTFSDNLKKKLPGNVTLKGPSGVVWNVGLTARDDTIYFTNGW-QRFVKD 65
W+ +N+ A FS + + L + + GPS WNVGLT+ +D GW Q+ V+D
Sbjct: 287 WQPLNVRAAPSAEFSVDRSRHLMSFLAMMGPSPD-WNVGLTS-EDLCTKECGWVQKVVQD 344
>ITBX_DROME (P11584) Integrin beta-PS precursor (Position-specific
antigen, beta chain) (Myospheroid protein) (Olfactory C
protein)
Length = 846
Score = 30.4 bits (67), Expect = 7.9
Identities = 14/45 (31%), Positives = 21/45 (46%)
Query: 252 CEKEASYFVGKCSHTQTELGGSKANETNNSIEEVNTASSDGGVEC 296
C + SYF KC + T+L AN+T+ + +T G C
Sbjct: 533 CNCDDSYFGNKCECSATDLTSKFANDTSCRADSTSTTDCSGRGHC 577
>GCSH_SHEON (Q8EIQ7) Glycine cleavage system H protein
Length = 129
Score = 30.4 bits (67), Expect = 7.9
Identities = 26/87 (29%), Positives = 39/87 (43%), Gaps = 11/87 (12%)
Query: 253 EKEASYFVGKCSHTQTELGGSKANETNNSIEEVNTASSDGGVECGLYEKFQDLNNIGTPL 312
E++ SY VG H Q LG E + EV + G +C + E + ++I P+
Sbjct: 20 EEDGSYTVGITEHAQELLGDMVFVE----LPEVGDTVT-AGDDCAVAESVKAASDIYAPI 74
Query: 313 AVPVETTNEKTFNAGVESDSPELLIAD 339
+ V NE DSPEL+ +D
Sbjct: 75 SGEVIAVNEAL------EDSPELVNSD 95
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.315 0.133 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,305,032
Number of Sequences: 164201
Number of extensions: 2227028
Number of successful extensions: 5009
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 4998
Number of HSP's gapped (non-prelim): 19
length of query: 385
length of database: 59,974,054
effective HSP length: 112
effective length of query: 273
effective length of database: 41,583,542
effective search space: 11352306966
effective search space used: 11352306966
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 67 (30.4 bits)
Medicago: description of AC148404.4