BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148360.15 - phase: 0
(422 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
GCS1_AQUAE (O67151) Glycine cleavage system H protein 1 37 0.073
HUL4_YEAST (P40985) Probable ubiquitin--protein ligase HUL4 (EC ... 36 0.16
IF2_CHLCV (Q823F2) Translation initiation factor IF-2 35 0.47
AIPL_HUMAN (Q9NZN9) Aryl-hydrocarbon interacting protein-like 1 34 0.81
CK13_YEAST (P39962) Casein kinase I homolog 3 (EC 2.7.1.-) 33 1.8
T1RE_ECOLI (Q47281) Type I restriction enzyme EcoEI R protein (E... 32 2.4
IF3A_YEAST (P38249) Eukaryotic translation initiation factor 3 1... 32 3.1
T1RA_ECOLI (Q07736) Type I restriction enzyme EcoAI R protein (E... 32 4.0
GPH_ECOLI (P32662) Phosphoglycolate phosphatase (EC 3.1.3.18) (PGP) 32 4.0
GPH_ECO57 (P58422) Phosphoglycolate phosphatase (EC 3.1.3.18) (PGP) 32 4.0
RUNT_DROME (P22814) Segmentation protein Runt 31 5.2
ZFH1_DROME (P28166) Zinc finger protein 1 (Zinc finger homeodoma... 31 6.8
Y45A_METJA (P81308) Hypothetical protein MJ0458.1 31 6.8
POGZ_HUMAN (Q7Z3K3) Pogo transposable element with ZNF domain (N... 31 6.8
MK21_YEAST (Q12176) Ribosome biogenesis protein MAK21 31 6.8
KAT1_HUMAN (Q16773) Kynurenine--oxoglutarate transaminase I (EC ... 31 6.8
GPH_SALTY (Q8ZLK5) Phosphoglycolate phosphatase (EC 3.1.3.18) (PGP) 31 6.8
GPH_KLEAE (Q9EYY5) Phosphoglycolate phosphatase (EC 3.1.3.18) (PGP) 31 6.8
GOA4_MOUSE (Q91VW5) Golgi autoantigen, golgin subfamily A member... 31 6.8
YF53_MYCBO (P64870) Hypothetical protein Mb1553c 30 8.9
>GCS1_AQUAE (O67151) Glycine cleavage system H protein 1
Length = 143
Score = 37.4 bits (85), Expect = 0.073
Identities = 22/85 (25%), Positives = 43/85 (49%), Gaps = 2/85 (2%)
Query: 52 KIRCSSCKAVVNAPSNLSKFPCPQCHVRIDVHADVEEVNEKIDSEPVISCFHKFSQTVSQ 111
K+ A++ A ++ FP P + +DV+ +V + E I+ +P S K T +
Sbjct: 53 KLEYDEALAIIEAGKRVATFPTPLSGIVVDVNEEVIKNPELINKKPYSSWIAKLKATNLE 112
Query: 112 ELDMLQNPRETVG--KQSVLVNEVE 134
E+ LQ+ +E V K +++ +V+
Sbjct: 113 EVKNLQSAKEIVKTVKDFIILEDVD 137
>HUL4_YEAST (P40985) Probable ubiquitin--protein ligase HUL4 (EC
6.3.2.-)
Length = 892
Score = 36.2 bits (82), Expect = 0.16
Identities = 22/76 (28%), Positives = 37/76 (47%), Gaps = 3/76 (3%)
Query: 48 SSAVKIRCSSCKAVVNAPSNLSKFPCPQCHVRIDVHADVEEVNEKIDSEPVISCFHKFSQ 107
S + + C C + P++++KF C C V + V E+N ++S ISC + Q
Sbjct: 77 SCLISLNCLCCGVPLRFPASITKFRCSACQVTVIVKE--PEINSNLESSTHISCTLEGLQ 134
Query: 108 TVSQEL-DMLQNPRET 122
V + D LQ ++T
Sbjct: 135 MVVRRCHDDLQRLKKT 150
>IF2_CHLCV (Q823F2) Translation initiation factor IF-2
Length = 887
Score = 34.7 bits (78), Expect = 0.47
Identities = 27/109 (24%), Positives = 49/109 (44%), Gaps = 10/109 (9%)
Query: 106 SQTVSQELDMLQNPRETVGKQSVLVNEVEQEEGDGGIAGETFTDYRPSKLSIGSPHPDPI 165
S VS++L+ Q P S + EE D + E ++S+ +P P P+
Sbjct: 93 SSFVSEDLNSPQPPVPVDSDASAFSDSAVVEEVDSFVETE-------QEISVSTP-PPPV 144
Query: 166 VETSSLSAVQPPEPTYDPKIKNDLERSKALSCLQIETLVYACQRHLQHV 214
E + + A +PP P +P++ E K S + I++ +H+ H+
Sbjct: 145 TEETEVVAKEPPAPKKEPEVVVKKEPPK--SVVSIKSNFGPTGKHINHL 191
>AIPL_HUMAN (Q9NZN9) Aryl-hydrocarbon interacting protein-like 1
Length = 384
Score = 33.9 bits (76), Expect = 0.81
Identities = 29/108 (26%), Positives = 46/108 (41%), Gaps = 2/108 (1%)
Query: 77 HVRIDVHADV-EEVNEKIDSEPVISCFHKFSQTVSQELDMLQNPRETVGKQSVLVNEVEQ 135
+VR HA+V E K D + V+ + V +EL +L+N ++ L
Sbjct: 268 YVRARAHAEVWNEAEAKADLQKVLELEPSMQKAVRRELRLLENRMAEKQEEERLRCRNML 327
Query: 136 EEGDGGIAGETFTDYRPSKLSIGSPHPDPIVETSSLSAVQPPEPTYDP 183
+G E T+ P++ S P P ++ LSA P EP +P
Sbjct: 328 SQGATQPPAEPPTE-PPAQSSTEPPAEPPTAPSAELSAGPPAEPATEP 374
>CK13_YEAST (P39962) Casein kinase I homolog 3 (EC 2.7.1.-)
Length = 524
Score = 32.7 bits (73), Expect = 1.8
Identities = 22/87 (25%), Positives = 37/87 (42%), Gaps = 16/87 (18%)
Query: 319 MQQLVQWCGPKFDGLIIFDECHKAKNLVPE-----------KDKKPTKTGQAVLDIQAQL 367
++ L +WCG KF + C AK ++ +D KP + Q
Sbjct: 111 LEDLFEWCGRKFS---VKTTCMVAKQMIDRVRAIHDHDLIYRDIKPDNF--LISQYQRIS 165
Query: 368 PEARVVYCSATGASEPRNMAYMVRLGL 394
PE +V+ A+ ++ N+ YMV G+
Sbjct: 166 PEGKVIKSCASSSNNDPNLIYMVDFGM 192
>T1RE_ECOLI (Q47281) Type I restriction enzyme EcoEI R protein (EC
3.1.21.3) (R.EcoEI)
Length = 813
Score = 32.3 bits (72), Expect = 2.4
Identities = 25/115 (21%), Positives = 43/115 (36%), Gaps = 18/115 (15%)
Query: 229 GVGKGRTVAGLIWENWHHGRRKTLWISVGSDLKFDA--RRDLDDMGASCISVHALNKLPY 286
G GK T +IW W +K + ++ D R D G +
Sbjct: 197 GTGKTYTAFQIIWRLWKAKNKKRILFLADRNILVDQTKRNDFQPFGNAM----------- 245
Query: 287 TKLDTKSVGVREGVIFSTYSSLIASSDRGRTRMQQLVQWCGPKFDGLIIFDECHK 341
TK+ +++ V + Y ++ + Q+ + P F LI+ DECH+
Sbjct: 246 TKVTGRTIDPAYEVHLALYQAITGPEEH-----QKAYKQVAPDFFDLIVIDECHR 295
>IF3A_YEAST (P38249) Eukaryotic translation initiation factor 3 110
kDa subunit (eIF3 p110) (Translation initiation factor
eIF3, p110 subunit) (eIF3a)
Length = 964
Score = 32.0 bits (71), Expect = 3.1
Identities = 30/127 (23%), Positives = 55/127 (42%), Gaps = 13/127 (10%)
Query: 87 EEVNEKIDSEPVISCFHKFSQTVSQELDMLQNPRETVGKQSVLVNEVEQEEGDGGIAGET 146
+EV+E+ ++EP + Q +E D P+ET E ++EE D I +
Sbjct: 505 KEVSEEENTEPEV-------QEEKEETDEALGPQETED------GEEKEEESDPVIIRNS 551
Query: 147 FTDYRPSKLSIGSPHPDPIVETSSLSAVQPPEPTYDPKIKNDLERSKALSCLQIETLVYA 206
+ + +LS D S + V+ T K K+DLE+ + +++
Sbjct: 552 YIHNKLLELSNVLHDVDSFNNASYMEKVRIARETLIKKNKDDLEKISKIVDERVKRSQEQ 611
Query: 207 CQRHLQH 213
Q+H++H
Sbjct: 612 KQKHMEH 618
>T1RA_ECOLI (Q07736) Type I restriction enzyme EcoAI R protein (EC
3.1.21.3) (R.EcoAI)
Length = 810
Score = 31.6 bits (70), Expect = 4.0
Identities = 24/115 (20%), Positives = 43/115 (36%), Gaps = 18/115 (15%)
Query: 229 GVGKGRTVAGLIWENWHHGRRKTLWISVGSDLKFDARR--DLDDMGASCISVHALNKLPY 286
G GK T +IW W +K + ++ D + D G +
Sbjct: 199 GTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDFQPFGTAM----------- 247
Query: 287 TKLDTKSVGVREGVIFSTYSSLIASSDRGRTRMQQLVQWCGPKFDGLIIFDECHK 341
TK+ +++ + + Y ++ G Q+ + P F LI+ DECH+
Sbjct: 248 TKVSGRTIDPAYEIHLALYQAIT-----GPEEDQKAFKQVAPDFFDLIVIDECHR 297
>GPH_ECOLI (P32662) Phosphoglycolate phosphatase (EC 3.1.3.18) (PGP)
Length = 252
Score = 31.6 bits (70), Expect = 4.0
Identities = 11/38 (28%), Positives = 21/38 (54%)
Query: 160 PHPDPIVETSSLSAVQPPEPTYDPKIKNDLERSKALSC 197
PHPDP++ + + P + + +ND++ +KA C
Sbjct: 168 PHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGC 205
>GPH_ECO57 (P58422) Phosphoglycolate phosphatase (EC 3.1.3.18) (PGP)
Length = 252
Score = 31.6 bits (70), Expect = 4.0
Identities = 11/38 (28%), Positives = 21/38 (54%)
Query: 160 PHPDPIVETSSLSAVQPPEPTYDPKIKNDLERSKALSC 197
PHPDP++ + + P + + +ND++ +KA C
Sbjct: 168 PHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGC 205
>RUNT_DROME (P22814) Segmentation protein Runt
Length = 509
Score = 31.2 bits (69), Expect = 5.2
Identities = 16/26 (61%), Positives = 18/26 (68%)
Query: 153 SKLSIGSPHPDPIVETSSLSAVQPPE 178
S S SP+P+P V TSS SAVQP E
Sbjct: 287 SSASSVSPNPNPSVATSSSSAVQPSE 312
>ZFH1_DROME (P28166) Zinc finger protein 1 (Zinc finger homeodomain
protein 1)
Length = 1054
Score = 30.8 bits (68), Expect = 6.8
Identities = 22/105 (20%), Positives = 43/105 (40%), Gaps = 3/105 (2%)
Query: 50 AVKIRCSSCKAVVNAPSNLSKFPCPQCHVRIDVHADVEEVNEKIDSEPVISCFHKFSQTV 109
A +RCS C N P+ L + C + + ++E+ ++ +
Sbjct: 625 AADLRCSRCSKQFNHPTELVQHEKVLCGL---IKEELEQHFQQQQATSFALASASEEDEE 681
Query: 110 SQELDMLQNPRETVGKQSVLVNEVEQEEGDGGIAGETFTDYRPSK 154
+E+D+ + PR+ G++ V V EE + + RPS+
Sbjct: 682 DEEMDVEEEPRQESGERKVRVRTAINEEQQQQLKQHYSLNARPSR 726
>Y45A_METJA (P81308) Hypothetical protein MJ0458.1
Length = 57
Score = 30.8 bits (68), Expect = 6.8
Identities = 14/60 (23%), Positives = 23/60 (38%), Gaps = 15/60 (25%)
Query: 17 CAGCRTYFSAAQGVAELPCPNCQMPHVFFVDSSAVKIRCSSCKAVVNAPSNLSKFPCPQC 76
C C + + + PCPNC + +RC C+ + N + CP+C
Sbjct: 8 CISCNAEIAPREKSTKFPCPNCGEVEI---------VRCERCRKLNN------PYKCPKC 52
>POGZ_HUMAN (Q7Z3K3) Pogo transposable element with ZNF domain
(Nbla00003)
Length = 1410
Score = 30.8 bits (68), Expect = 6.8
Identities = 31/149 (20%), Positives = 52/149 (34%), Gaps = 19/149 (12%)
Query: 7 PPPPSTARARCAGCRTYFSAAQGVAELPCPN------CQMPHVFFVDSSAVKIRCSSCKA 60
P P TA T A ++P PN Q + VD
Sbjct: 424 PSPEKTAPVASTPSSTPIPALSPPTKVPEPNENVGDAVQTKLIMLVDDFYYGRDGGKVAQ 483
Query: 61 VVNAPSNLSKFPCPQCHVRI----------DVHADVEEVNEKIDSEPVIS-CFHKFSQTV 109
+ N P + F CP C R+ H ++++ N ++D + C+ +FS
Sbjct: 484 LTNFPKVATSFRCPHCTKRLKNNIRFMNHMKHHVELDQQNGEVDGHTICQHCYRQFSTPF 543
Query: 110 SQE--LDMLQNPRETVGKQSVLVNEVEQE 136
+ L+ + +P E+ K + E E
Sbjct: 544 QLQCHLENVHSPYESTTKCKICEWAFESE 572
>MK21_YEAST (Q12176) Ribosome biogenesis protein MAK21
Length = 1025
Score = 30.8 bits (68), Expect = 6.8
Identities = 26/121 (21%), Positives = 47/121 (38%), Gaps = 5/121 (4%)
Query: 105 FSQTVSQELDMLQNPRETVGKQSVLVNEVEQEEGDGGIAGETFTDYRPSKLSIGSPHPDP 164
F Q +++D E+ + + E+E IA T + GS +
Sbjct: 110 FDQHKLEDVDDDDIEEESTSSKESKIPAQEKEHAQSNIASSTIEKTSQESIDNGSEQEEN 169
Query: 165 IVETSSLSAVQPPEPTYDPKIKNDLERSKAL----SCLQIETLVYACQRHLQHVPSGPRA 220
VE ++LS+ Q PE K K + E+ L + + + L+ + +P P+
Sbjct: 170 TVEEANLSSDQEPESESAEKEKKE-EKDGGLITQTTIISSDKLIIPYDKPWYEIPLDPQV 228
Query: 221 G 221
G
Sbjct: 229 G 229
>KAT1_HUMAN (Q16773) Kynurenine--oxoglutarate transaminase I (EC
2.6.1.7) (Kynurenine aminotransferase I) (KATI)
(Glutamine--phenylpyruvate transaminase) (EC 2.6.1.64)
(Glutamine transaminase K) (GTK) (Cysteine-S-conjugate
beta-lyase) (EC 4.4.1.13)
Length = 422
Score = 30.8 bits (68), Expect = 6.8
Identities = 31/115 (26%), Positives = 45/115 (38%), Gaps = 9/115 (7%)
Query: 53 IRCSSCKAVVNAPSNLSKFPCPQCHVRIDVHAD-----VEEVNEKIDSEPVISCFHKF-S 106
++ +S VVN FP P V HA + + + P+ F
Sbjct: 22 VKLASEHDVVNLGQGFPDFPPPDFAVEAFQHAVSGDFMLNQYTKTFGYPPLTKILASFFG 81
Query: 107 QTVSQELDMLQNPRETVGKQSVLVNEVEQ--EEGDGGIAGETFTD-YRPSKLSIG 158
+ + QE+D L+N TVG L + +EGD I E F D Y P + G
Sbjct: 82 ELLGQEIDPLRNVLVTVGGYGALFTAFQALVDEGDEVIIIEPFFDCYEPMTMMAG 136
>GPH_SALTY (Q8ZLK5) Phosphoglycolate phosphatase (EC 3.1.3.18) (PGP)
Length = 252
Score = 30.8 bits (68), Expect = 6.8
Identities = 11/38 (28%), Positives = 22/38 (56%)
Query: 160 PHPDPIVETSSLSAVQPPEPTYDPKIKNDLERSKALSC 197
PHP+P++ +S + P + + +ND++ +KA C
Sbjct: 168 PHPEPLLLVASRLGMMPEQMLFVGDSRNDIQAAKAAGC 205
>GPH_KLEAE (Q9EYY5) Phosphoglycolate phosphatase (EC 3.1.3.18) (PGP)
Length = 253
Score = 30.8 bits (68), Expect = 6.8
Identities = 11/41 (26%), Positives = 23/41 (55%)
Query: 160 PHPDPIVETSSLSAVQPPEPTYDPKIKNDLERSKALSCLQI 200
PHP+P++ + ++ P E + +ND++ +KA C +
Sbjct: 169 PHPEPLLLVAEKLSLAPAELLFVGDSRNDIQAAKAAGCCSV 209
>GOA4_MOUSE (Q91VW5) Golgi autoantigen, golgin subfamily A member 4
(tGolgin-1)
Length = 2238
Score = 30.8 bits (68), Expect = 6.8
Identities = 24/87 (27%), Positives = 42/87 (47%), Gaps = 10/87 (11%)
Query: 63 NAPSNL-SKFPCPQCHVRIDVHADVEE---VNEKIDSEPVISCFHKFSQTVSQELDMLQN 118
N P N+ + P P ++ +VH D E+ V E + Q V ++ ++LQ
Sbjct: 258 NGPMNVDAPKPLPPGELQAEVHGDTEKMEGVGEPVGGGTSAKTLEMLQQRVKRQENLLQR 317
Query: 119 PRETVGKQ----SVLVNEVE--QEEGD 139
+ET+G ++L++E E QE+ D
Sbjct: 318 CKETIGSHKEQCALLLSEKEALQEQLD 344
>YF53_MYCBO (P64870) Hypothetical protein Mb1553c
Length = 426
Score = 30.4 bits (67), Expect = 8.9
Identities = 15/50 (30%), Positives = 26/50 (52%)
Query: 353 PTKTGQAVLDIQAQLPEARVVYCSATGASEPRNMAYMVRLGLWGAGTFFP 402
P +T + D+ AQL E ++Y A ++ R+ ++ R+GL T P
Sbjct: 263 PVQTLAMISDVCAQLGERALIYSPAANSTRIRHADHVKRVGLVNYSTILP 312
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.136 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,135,897
Number of Sequences: 164201
Number of extensions: 2373107
Number of successful extensions: 6531
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 6510
Number of HSP's gapped (non-prelim): 33
length of query: 422
length of database: 59,974,054
effective HSP length: 113
effective length of query: 309
effective length of database: 41,419,341
effective search space: 12798576369
effective search space used: 12798576369
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 67 (30.4 bits)
Medicago: description of AC148360.15