BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148289.7 - phase: 0
(197 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CPSD_HUMAN (O95639) Cleavage and polyadenylation specificity fac... 43 5e-04
MKR1_MOUSE (Q9QXP6) Makorin 1 41 0.002
CPSD_MOUSE (Q8BQZ5) Cleavage and polyadenylation specificity fac... 39 0.006
MKR2_SERQU (Q9DD48) Makorin 2 (Zinc finger protein YGHLC3HC4) 39 0.010
TTP_HUMAN (P26651) Tristetraproline (TTP) (Zinc finger protein 3... 37 0.038
TIS1_DROME (P47980) TIS11 protein (dTIS11) 37 0.038
YQC1_SCHPO (O74463) Hypothetical protein C1739.01 in chromosome III 36 0.065
MKR1_MACEU (Q9TT91) Makorin 1 36 0.065
LEE1_YEAST (Q02799) Zinc finger protein LEE1 36 0.065
TISD_MOUSE (P23949) Butyrate response factor 2 (TIS11D protein) 35 0.085
TISD_HUMAN (P47974) Butyrate response factor 2 (TIS11D protein) ... 35 0.085
MKR1_HUMAN (Q9UHC7) Makorin 1 35 0.11
TTP_RAT (P47973) Tristetraproline (TTP) (Zinc finger protein 36)... 35 0.15
SCP3_SCHPO (P41000) Protein scp3 35 0.15
TISB_RAT (P17431) Butyrate response factor 1 (TIS11B protein) (E... 34 0.19
TISB_MOUSE (P23950) Butyrate response factor 1 (TIS11B protein) 34 0.19
TISB_HUMAN (Q07352) Butyrate response factor 1 (TIS11B protein) ... 34 0.19
MKR2_MOUSE (Q9ERV1) Makorin 2 34 0.19
MKR2_HUMAN (Q9H000) Makorin 2 (HSPC070) 34 0.25
MKR2_BRARE (Q9DFG8) Makorin 2 33 0.32
>CPSD_HUMAN (O95639) Cleavage and polyadenylation specificity
factor, 30 kDa subunit (CPSF 30 kDa subunit) (NS1
effector domain-binding protein 1) (Neb-1) (No arches
homolog)
Length = 269
Score = 42.7 bits (99), Expect = 5e-04
Identities = 29/76 (38%), Positives = 32/76 (41%), Gaps = 9/76 (11%)
Query: 61 PECQFYMKTGDCKFGAVCRFHH--PRERTIPAP-----DCVLSPLGLPLRPGEPLCVFYS 113
PEC FY K G+C C F H P + P C PL +CV Y
Sbjct: 94 PECYFYSKFGECS-NKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 152
Query: 114 RYGICKFGPSCKFDHP 129
G C GPSCKF HP
Sbjct: 153 -VGFCPEGPSCKFMHP 167
>MKR1_MOUSE (Q9QXP6) Makorin 1
Length = 481
Score = 41.2 bits (95), Expect = 0.002
Identities = 32/112 (28%), Positives = 51/112 (44%), Gaps = 19/112 (16%)
Query: 60 QPECQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICK 119
Q C+++M G CK G CR+ H D SP G+ +C ++ R G C
Sbjct: 58 QVTCRYFMH-GVCKEGDNCRYSH---------DLSDSPYGV-------VCKYFQR-GYCV 99
Query: 120 FGPSCKFDHPMGIFTYNVSASPLAEAAGRRLLGS-SSGTAALSLSSEGLVES 170
+G C+++H + V+A+ L+ S SSG +L+ + G ES
Sbjct: 100 YGDRCRYEHSKPLKQEEVTATDLSAKPSLAASSSLSSGVGSLAEMNSGEAES 151
>CPSD_MOUSE (Q8BQZ5) Cleavage and polyadenylation specificity
factor, 30 kDa subunit (CPSF 30 kDa subunit) (Clipper
homolog) (Clipper/CPSF 30K)
Length = 211
Score = 39.3 bits (90), Expect = 0.006
Identities = 26/71 (36%), Positives = 33/71 (45%), Gaps = 7/71 (9%)
Query: 63 CQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVL----SPLGLPLRPGEPLCVFYSRYGIC 118
C+ +++ G CK G C F H + T P+C PL +CV Y G C
Sbjct: 68 CKHWLR-GLCKKGDQCEFLHEYDMT-KMPECYFYSKFGPLCRHRHTRRVICVNYL-VGFC 124
Query: 119 KFGPSCKFDHP 129
GPSCKF HP
Sbjct: 125 PEGPSCKFMHP 135
Score = 29.3 bits (64), Expect = 6.1
Identities = 25/87 (28%), Positives = 35/87 (39%), Gaps = 22/87 (25%)
Query: 63 CQFYMKTGDCKFGAVCRFHH-PRERTIPAPDCVLSPLGLPLRPGE------------PLC 109
C+F++K C G +C F H E+T+ C GL + + P C
Sbjct: 41 CEFFLKAA-CGKGGMCPFRHISGEKTVV---CKHWLRGLCKKGDQCEFLHEYDMTKMPEC 96
Query: 110 VFYSRYGICKFGPSCKFDHPMGIFTYN 136
FYS KFGP C+ H + N
Sbjct: 97 YFYS-----KFGPLCRHRHTRRVICVN 118
>MKR2_SERQU (Q9DD48) Makorin 2 (Zinc finger protein YGHLC3HC4)
Length = 423
Score = 38.5 bits (88), Expect = 0.010
Identities = 28/115 (24%), Positives = 47/115 (40%), Gaps = 21/115 (18%)
Query: 60 QPECQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICK 119
Q C++++ G C+ G+ C F H + P+ +C FY R G+C
Sbjct: 5 QVTCRYFLH-GVCREGSRCLFSHDLNNSKPST----------------ICKFYQR-GVCA 46
Query: 120 FGPSCKFDHPMGIFTYNVSASPLAEAAGRRLLGSSSGTAALSLSSEGLVESGSVK 174
+G C++DH +P +A G G+ G A + + G G +K
Sbjct: 47 YGERCRYDHIKPSSRGGGGGAPEDQAGGG---GAGGGGAGIGGAGGGPSVRGGMK 98
>TTP_HUMAN (P26651) Tristetraproline (TTP) (Zinc finger protein 36
homolog) (Zfp-36) (TIS11A protein) (TIS11) (Growth
factor-inducible nuclear protein NUP475) (G0/G1 switch
regulatory protein 24)
Length = 326
Score = 36.6 bits (83), Expect = 0.038
Identities = 34/131 (25%), Positives = 48/131 (35%), Gaps = 20/131 (15%)
Query: 55 PERPDQPECQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSR 114
P R C+ + ++G C++GA C+F H L + LC +
Sbjct: 101 PSRYKTELCRTFSESGRCRYGAKCQFAHGLGE--------LRQANRHPKYKTELCHKFYL 152
Query: 115 YGICKFGPSCKFDHPMG----------IFTYNVSASPLAEAAGRRLLGSSSGTAALSLSS 164
G C +G C F H + ++S S L +GRR G A SLSS
Sbjct: 153 QGRCPYGSRCHFIHNPSEDLAAPGHPPVLRQSISFSGL--PSGRRTSPPPPGLAGPSLSS 210
Query: 165 EGLVESGSVKP 175
S S P
Sbjct: 211 SSFSPSSSPPP 221
Score = 32.0 bits (71), Expect = 0.94
Identities = 14/43 (32%), Positives = 21/43 (48%)
Query: 89 PAPDCVLSPLGLPLRPGEPLCVFYSRYGICKFGPSCKFDHPMG 131
P+P + P R LC +S G C++G C+F H +G
Sbjct: 89 PSPTSPTATSTTPSRYKTELCRTFSESGRCRYGAKCQFAHGLG 131
>TIS1_DROME (P47980) TIS11 protein (dTIS11)
Length = 437
Score = 36.6 bits (83), Expect = 0.038
Identities = 33/151 (21%), Positives = 54/151 (34%), Gaps = 24/151 (15%)
Query: 48 LQRENIFPERPDQPE---------CQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPL 98
L+R P P QP C+ + + G+CK+G C+F H L +
Sbjct: 118 LERTQSEPLPPQQPMNTSRYKTELCRPFEEAGECKYGEKCQFAHGSHE--------LRNV 169
Query: 99 GLPLRPGEPLCVFYSRYGICKFGPSCKFDHPMGIFTYNVSASPLAEAAGRRLLGSSSGTA 158
+ C + G C +GP C F H N + +AA + S +
Sbjct: 170 HRHPKYKTEYCRTFHSVGFCPYGPRCHFVH-------NADEARAQQAAQAAKSSTQSQSQ 222
Query: 159 ALSLSSEGLVESGSVKPRRLSLSETRPNPSG 189
+ SS+ + + S S + + SG
Sbjct: 223 SQQSSSQNFSPKSNQSSNQSSNSSSSSSSSG 253
>YQC1_SCHPO (O74463) Hypothetical protein C1739.01 in chromosome III
Length = 547
Score = 35.8 bits (81), Expect = 0.065
Identities = 33/133 (24%), Positives = 49/133 (36%), Gaps = 30/133 (22%)
Query: 63 CQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICKFGP 122
C+F+ + G C G C F H E P+C ++ + G CKFGP
Sbjct: 47 CKFF-RNGTCTAGENCPFSHSLETE------------------RPICKYFLK-GNCKFGP 86
Query: 123 SCKFDH--------PMGIFTYNVSASPLAEAAGRRLLGSSSGTAALSLS-SEGLVESGSV 173
C H P G T N AS A + G +S S S +++ +
Sbjct: 87 KCALSHALPGNTNLPNGTST-NTMASMAANGGASSVASKQMGANQISPSLSSKTMKNPAD 145
Query: 174 KPRRLSLSETRPN 186
K + ++ R N
Sbjct: 146 KANNTTATDVRGN 158
>MKR1_MACEU (Q9TT91) Makorin 1
Length = 478
Score = 35.8 bits (81), Expect = 0.065
Identities = 28/101 (27%), Positives = 43/101 (41%), Gaps = 21/101 (20%)
Query: 60 QPECQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICK 119
Q C+++M G CK G CR+ H L +C +Y R G C
Sbjct: 54 QVTCRYFMH-GVCKKGNNCRYSHD----------------LSTSQSAMVCRYYQR-GCCA 95
Query: 120 FGPSCKFDHPMGIFTYNVSASPLAEAAGRRLLGSSSGTAAL 160
+G C+++H + V+A+ LA + L +SS AL
Sbjct: 96 YGDRCRYEHTKPLKREEVTAANLAAKSD---LPASSSLPAL 133
>LEE1_YEAST (Q02799) Zinc finger protein LEE1
Length = 301
Score = 35.8 bits (81), Expect = 0.065
Identities = 22/66 (33%), Positives = 33/66 (49%), Gaps = 16/66 (24%)
Query: 63 CQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICKFGP 122
C+F+ K G+C+ G+ C F H +PD + S LP C ++++ G CKFG
Sbjct: 93 CKFF-KMGNCQAGSSCPFSH-------SPDIISSANNLP-------CKYFAK-GNCKFGN 136
Query: 123 SCKFDH 128
C H
Sbjct: 137 KCVNAH 142
>TISD_MOUSE (P23949) Butyrate response factor 2 (TIS11D protein)
Length = 367
Score = 35.4 bits (80), Expect = 0.085
Identities = 19/66 (28%), Positives = 28/66 (41%), Gaps = 8/66 (12%)
Query: 63 CQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICKFGP 122
C+ + ++G CK+G C+F H L L + LC + G C +GP
Sbjct: 132 CRPFEESGTCKYGEKCQFAHGFHE--------LRSLTRHPKYKTELCRTFHTIGFCPYGP 183
Query: 123 SCKFDH 128
C F H
Sbjct: 184 RCHFIH 189
>TISD_HUMAN (P47974) Butyrate response factor 2 (TIS11D protein)
(EGF-response factor 2) (ERF-2)
Length = 492
Score = 35.4 bits (80), Expect = 0.085
Identities = 19/66 (28%), Positives = 28/66 (41%), Gaps = 8/66 (12%)
Query: 63 CQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICKFGP 122
C+ + ++G CK+G C+F H L L + LC + G C +GP
Sbjct: 159 CRPFEESGTCKYGEKCQFAHGFHE--------LRSLTRHPKYKTELCRTFHTIGFCPYGP 210
Query: 123 SCKFDH 128
C F H
Sbjct: 211 RCHFIH 216
>MKR1_HUMAN (Q9UHC7) Makorin 1
Length = 482
Score = 35.0 bits (79), Expect = 0.11
Identities = 31/107 (28%), Positives = 44/107 (40%), Gaps = 30/107 (28%)
Query: 60 QPECQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICK 119
Q C+++M G CK G CR+ H L P +C ++ R G C
Sbjct: 58 QVTCRYFMH-GVCKEGDNCRYSHD----------------LSDSPYSVVCKYFQR-GYCI 99
Query: 120 FGPSCKFDHPMGIFTYNVSASPL--AEAAGRRLLGSSSGTAALSLSS 164
+G C+++H + PL EA L SS A+ SLSS
Sbjct: 100 YGDRCRYEH----------SKPLKQEEATATELTTKSSLAASSSLSS 136
>TTP_RAT (P47973) Tristetraproline (TTP) (Zinc finger protein 36)
(Zfp-36) (TIS11A protein) (TIS11)
Length = 320
Score = 34.7 bits (78), Expect = 0.15
Identities = 34/122 (27%), Positives = 45/122 (36%), Gaps = 18/122 (14%)
Query: 63 CQFYMKTGDCKFGAVCRF-HHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICKFG 121
C+ Y ++G C++GA C+F H P E L + LC + G C +G
Sbjct: 102 CRTYSESGRCRYGAKCQFAHGPGE---------LRQANRHPKYKTELCHKFYLQGRCPYG 152
Query: 122 PSCKFDH--------PMGIFTYNVSASPLAEAAGRRLLGSSSGTAALSLSSEGLVESGSV 173
C F H P S S +GRR G + SLSS S S
Sbjct: 153 SRCHFIHNPTEDLALPGQPHVLRQSISFSGLPSGRRTSPPPPGFSGPSLSSCSFSPSSSP 212
Query: 174 KP 175
P
Sbjct: 213 PP 214
Score = 28.9 bits (63), Expect = 8.0
Identities = 11/24 (45%), Positives = 14/24 (57%)
Query: 108 LCVFYSRYGICKFGPSCKFDHPMG 131
LC YS G C++G C+F H G
Sbjct: 101 LCRTYSESGRCRYGAKCQFAHGPG 124
>SCP3_SCHPO (P41000) Protein scp3
Length = 583
Score = 34.7 bits (78), Expect = 0.15
Identities = 34/150 (22%), Positives = 53/150 (34%), Gaps = 39/150 (26%)
Query: 55 PERPDQPECQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSR 114
P+ C+F+ + G C G C F H E L + +C ++ +
Sbjct: 33 PKSLQHVPCKFF-RQGTCTSGKNCIFSHDLE----------------LATEKTICKYFQK 75
Query: 115 YGICKFGPSCKFDH------------------PMGIFTYNVSASPLAEAAGRRLLGSSSG 156
G CKFG C +H MG + N+SA+P+A
Sbjct: 76 -GNCKFGSKCALEHVLPDGRKVKTRAFAPSTTAMGSSSQNISAAPMANIISNNDKILPMT 134
Query: 157 TAALSLSSEGLVESGSVKPRRLSLSETRPN 186
T A + +SE E +K L + + N
Sbjct: 135 TMASATASE---EKNRIKDEALVIKKEESN 161
>TISB_RAT (P17431) Butyrate response factor 1 (TIS11B protein)
(EGF-inducible protein CMG1)
Length = 338
Score = 34.3 bits (77), Expect = 0.19
Identities = 19/66 (28%), Positives = 27/66 (40%), Gaps = 8/66 (12%)
Query: 63 CQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICKFGP 122
C+ + + G CK+G C+F H L L + LC + G C +GP
Sbjct: 120 CRPFEENGACKYGDKCQFAHGIHE--------LRSLTRHPKYKTELCRTFHTIGFCPYGP 171
Query: 123 SCKFDH 128
C F H
Sbjct: 172 RCHFIH 177
>TISB_MOUSE (P23950) Butyrate response factor 1 (TIS11B protein)
Length = 338
Score = 34.3 bits (77), Expect = 0.19
Identities = 19/66 (28%), Positives = 27/66 (40%), Gaps = 8/66 (12%)
Query: 63 CQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICKFGP 122
C+ + + G CK+G C+F H L L + LC + G C +GP
Sbjct: 120 CRPFEENGACKYGDKCQFAHGIHE--------LRSLTRHPKYKTELCRTFHTIGFCPYGP 171
Query: 123 SCKFDH 128
C F H
Sbjct: 172 RCHFIH 177
>TISB_HUMAN (Q07352) Butyrate response factor 1 (TIS11B protein)
(EGF-response factor 1) (ERF-1)
Length = 338
Score = 34.3 bits (77), Expect = 0.19
Identities = 19/66 (28%), Positives = 27/66 (40%), Gaps = 8/66 (12%)
Query: 63 CQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICKFGP 122
C+ + + G CK+G C+F H L L + LC + G C +GP
Sbjct: 120 CRPFEENGACKYGDKCQFAHGIHE--------LRSLTRHPKYKTELCRTFHTIGFCPYGP 171
Query: 123 SCKFDH 128
C F H
Sbjct: 172 RCHFIH 177
>MKR2_MOUSE (Q9ERV1) Makorin 2
Length = 416
Score = 34.3 bits (77), Expect = 0.19
Identities = 18/69 (26%), Positives = 31/69 (44%), Gaps = 18/69 (26%)
Query: 60 QPECQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICK 119
Q C+++M G C+ G+ C F H + P+ +C +Y + G C
Sbjct: 5 QVTCRYFMH-GVCREGSQCLFSHDLANSKPST----------------ICKYYQK-GYCA 46
Query: 120 FGPSCKFDH 128
+G C++DH
Sbjct: 47 YGARCRYDH 55
>MKR2_HUMAN (Q9H000) Makorin 2 (HSPC070)
Length = 416
Score = 33.9 bits (76), Expect = 0.25
Identities = 18/69 (26%), Positives = 31/69 (44%), Gaps = 18/69 (26%)
Query: 60 QPECQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICK 119
Q C+++M G C+ G+ C F H + P+ +C +Y + G C
Sbjct: 5 QITCRYFMH-GVCREGSQCLFSHDLANSKPST----------------ICKYYQK-GYCA 46
Query: 120 FGPSCKFDH 128
+G C++DH
Sbjct: 47 YGTRCRYDH 55
>MKR2_BRARE (Q9DFG8) Makorin 2
Length = 414
Score = 33.5 bits (75), Expect = 0.32
Identities = 18/69 (26%), Positives = 31/69 (44%), Gaps = 18/69 (26%)
Query: 60 QPECQFYMKTGDCKFGAVCRFHHPRERTIPAPDCVLSPLGLPLRPGEPLCVFYSRYGICK 119
Q C++++ G C+ G+ C F H + P+ +C +Y R G C
Sbjct: 5 QVTCRYFLH-GVCREGSRCLFSHDLTTSKPST----------------ICKYYQR-GACA 46
Query: 120 FGPSCKFDH 128
+G C++DH
Sbjct: 47 YGDRCRYDH 55
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.137 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,479,741
Number of Sequences: 164201
Number of extensions: 1175787
Number of successful extensions: 2528
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 34
Number of HSP's that attempted gapping in prelim test: 2396
Number of HSP's gapped (non-prelim): 126
length of query: 197
length of database: 59,974,054
effective HSP length: 105
effective length of query: 92
effective length of database: 42,732,949
effective search space: 3931431308
effective search space used: 3931431308
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC148289.7