BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147961.4 - phase: 0
(266 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YH11_MYCLE (O05668) Hypothetical pseudouridine synthase ML1370 (... 37 0.050
HMDH_SCHMA (P16237) 3-hydroxy-3-methylglutaryl-coenzyme A reduct... 35 0.25
YH38_MYCBO (P65843) Hypothetical pseudouridine synthase Mb1738 (... 32 1.2
YH11_MYCTU (P65842) Hypothetical pseudouridine synthase Rv1711/M... 32 1.2
HYIN_AGRRH (Q09102) Indoleacetamide hydrolase (EC 3.5.1.-) (IAH)... 32 1.6
MD12_YEAST (Q92328) Mitochondrial inheritance component MDM12 31 2.7
RIK2_MOUSE (P58801) Receptor-interacting serine/threonine-protei... 31 3.6
LEU2_BUCUO (Q9EVI0) 3-isopropylmalate dehydratase large subunit ... 31 3.6
SSY5_YEAST (P47002) SSY5 protein 30 4.7
LEU2_BUCAP (O85065) 3-isopropylmalate dehydratase large subunit ... 30 4.7
UVRC_NEIMB (Q9JZ26) UvrABC system protein C (UvrC protein) (Exci... 30 6.1
UVRC_NEIMA (Q9JU21) UvrABC system protein C (UvrC protein) (Exci... 30 6.1
UL71_HHV6Z (P52546) Protein U44 30 6.1
LEU2_BUCRP (P48573) 3-isopropylmalate dehydratase large subunit ... 30 6.1
ICA1_MOUSE (P13597) Intercellular adhesion molecule-1 precursor ... 30 6.1
>YH11_MYCLE (O05668) Hypothetical pseudouridine synthase ML1370 (EC
4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase)
Length = 256
Score = 37.0 bits (84), Expect = 0.050
Identities = 42/140 (30%), Positives = 61/140 (43%), Gaps = 17/140 (12%)
Query: 134 IVQDTTVGSATNENAKICGVMLTD-GSICKRPPVEKRVRCPE---HKGMRINASTTKAIR 189
+V D+ V A N+ + M D G C +E+RVR + H G R++A T I
Sbjct: 71 VVDDSLVYLALNKPRGMYSTMSDDRGRPCVGDLIERRVRGNKKLFHVG-RLDADTEGLIL 129
Query: 190 SPKSELESIVRNAYRYQNASHDVEDPPQRTVKCHVEEGITKTIICGIILDDG-------S 242
L + A+R + SH+V TVK V G+ K + G+ LDDG +
Sbjct: 130 -----LTNDGELAHRLMHPSHEVSKTYLATVKGAVPRGLGKKLSVGLELDDGPAHVDDFA 184
Query: 243 TCRRQPVKGRKRCQEHKGRR 262
P K R H+GR+
Sbjct: 185 VVDAIPGKTLVRLTLHEGRK 204
>HMDH_SCHMA (P16237) 3-hydroxy-3-methylglutaryl-coenzyme A reductase
(EC 1.1.1.34) (HMG-CoA reductase)
Length = 948
Score = 34.7 bits (78), Expect = 0.25
Identities = 36/124 (29%), Positives = 54/124 (43%), Gaps = 28/124 (22%)
Query: 86 ILP-FTQKKVGIPIKSSK----LHLTDDKLDEADSGSYNVLSRVFKFNRSRPRIVQDTTV 140
+LP F++K IP++S K LH DD +D DS S + S K + RP V D +
Sbjct: 398 VLPKFSKKLNDIPLQSRKRIYCLHKDDDYIDRNDSSSVSTFSNTCKNSNERPSNVLD--L 455
Query: 141 GSATNENAKICGVMLTDGSICKRPPVEKRVRCPEHKGMRINASTTKAIRSPKSELESIVR 200
T + + G L+D I ++ H ++ ELES+VR
Sbjct: 456 DMLTEKIKQGLGHELSDTEI---------LQLLSHGRLKTR------------ELESVVR 494
Query: 201 NAYR 204
N +R
Sbjct: 495 NPFR 498
>YH38_MYCBO (P65843) Hypothetical pseudouridine synthase Mb1738 (EC
4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase)
Length = 254
Score = 32.3 bits (72), Expect = 1.2
Identities = 33/112 (29%), Positives = 52/112 (45%), Gaps = 10/112 (8%)
Query: 134 IVQDTTVGSATNENAKICGVMLTD-GSICKRPPVEKRVRCPE---HKGMRINASTTKAIR 189
++ D+ V A N+ + M D G C +E++VR + H G R++A T +
Sbjct: 69 VLDDSLVYLALNKPRGMHSTMSDDRGRPCIGDLIERKVRGTKKLFHVG-RLDADTEGLML 127
Query: 190 SPKSELESIVRNAYRYQNASHDVEDPPQRTVKCHVEEGITKTIICGIILDDG 241
L + A+R + SH+V TV V G+ +T+ GI LDDG
Sbjct: 128 -----LTNDGELAHRLMHPSHEVPKTYLATVTGSVPRGLGRTLRAGIELDDG 174
>YH11_MYCTU (P65842) Hypothetical pseudouridine synthase
Rv1711/MT1751.1 (EC 4.2.1.70) (Pseudouridylate synthase)
(Uracil hydrolyase)
Length = 254
Score = 32.3 bits (72), Expect = 1.2
Identities = 33/112 (29%), Positives = 52/112 (45%), Gaps = 10/112 (8%)
Query: 134 IVQDTTVGSATNENAKICGVMLTD-GSICKRPPVEKRVRCPE---HKGMRINASTTKAIR 189
++ D+ V A N+ + M D G C +E++VR + H G R++A T +
Sbjct: 69 VLDDSLVYLALNKPRGMHSTMSDDRGRPCIGDLIERKVRGTKKLFHVG-RLDADTEGLML 127
Query: 190 SPKSELESIVRNAYRYQNASHDVEDPPQRTVKCHVEEGITKTIICGIILDDG 241
L + A+R + SH+V TV V G+ +T+ GI LDDG
Sbjct: 128 -----LTNDGELAHRLMHPSHEVPKTYLATVTGSVPRGLGRTLRAGIELDDG 174
>HYIN_AGRRH (Q09102) Indoleacetamide hydrolase (EC 3.5.1.-) (IAH)
(Indole-3-acetamide hydrolase)
Length = 466
Score = 32.0 bits (71), Expect = 1.6
Identities = 17/32 (53%), Positives = 20/32 (62%), Gaps = 3/32 (9%)
Query: 153 VMLTDGSICKRPPVEKRVRCPEHKGMRINAST 184
V+L DG IC RPPV + VR KG+RI T
Sbjct: 218 VILLDGIICGRPPVNQTVRL---KGLRIGLPT 246
>MD12_YEAST (Q92328) Mitochondrial inheritance component MDM12
Length = 271
Score = 31.2 bits (69), Expect = 2.7
Identities = 23/67 (34%), Positives = 33/67 (48%), Gaps = 4/67 (5%)
Query: 47 TFDYAWNTISNGTRRPDDIHQMLNKLASGTRTFSDVAKL-ILPFTQKKVGIPIKSSKLHL 105
+FD W+T+ + R D I + LN T+ S V+ L +L F KVG I ++
Sbjct: 2 SFDINWSTLESDNRLNDLIRKHLNSYLQNTQLPSYVSNLRVLDFDLGKVGPAITLKEI-- 59
Query: 106 TDDKLDE 112
D LDE
Sbjct: 60 -TDPLDE 65
>RIK2_MOUSE (P58801) Receptor-interacting serine/threonine-protein
kinase 2 (EC 2.7.1.37)
Length = 539
Score = 30.8 bits (68), Expect = 3.6
Identities = 22/68 (32%), Positives = 30/68 (43%), Gaps = 3/68 (4%)
Query: 44 LLSTFDYAWNTISNGTRRPDDIHQMLNKLASGTRTFSDVAKLILPFTQKKVGIPIKSSKL 103
++S W N RP + + L +L RTF D+ L KK I SS +
Sbjct: 265 MISLIQSGW--AQNPDERPSFL-KCLIELEPVLRTFEDITFLEAVIQLKKAKIQSSSSTI 321
Query: 104 HLTDDKLD 111
HL D K+D
Sbjct: 322 HLCDKKMD 329
>LEU2_BUCUO (Q9EVI0) 3-isopropylmalate dehydratase large subunit (EC
4.2.1.33) (Isopropylmalate isomerase) (Alpha-IPM
isomerase) (IPMI) (Fragment)
Length = 443
Score = 30.8 bits (68), Expect = 3.6
Identities = 21/62 (33%), Positives = 35/62 (55%), Gaps = 12/62 (19%)
Query: 180 INASTTKAIRSPKSELESIVRNAYRYQNASHDVEDPPQRTVKCHV---EEGIT---KTII 233
INAS + A K ++E +++N + + +D+ +P Q V HV E+G+T TI+
Sbjct: 72 INASGSMA----KKQMEQLIKNCQEFNISLYDINNPNQGIV--HVIAPEKGMTLPGMTIV 125
Query: 234 CG 235
CG
Sbjct: 126 CG 127
>SSY5_YEAST (P47002) SSY5 protein
Length = 687
Score = 30.4 bits (67), Expect = 4.7
Identities = 29/118 (24%), Positives = 48/118 (40%), Gaps = 14/118 (11%)
Query: 63 DDIHQMLNKLASGTRTFSDVAKLILPFTQKKVGIPIKSSK-----------LHLTDDKL- 110
DDIHQ + ++ + K LP T+ ++ I K LH D+ L
Sbjct: 189 DDIHQDVISISKAVIEAIEYFKEFLPTTRDRIPYRISLEKSSSLRKINKIVLHFLDNLLV 248
Query: 111 DEADSGSYNVLSRVFKFNRSRPRIVQDTTVGSATNENAKICGVMLTDGSICKRPPVEK 168
+A S S ++L R F F + ++ D + + C + GS C P ++K
Sbjct: 249 SDAFSNSRSILLRRFYFFLKKLNLITDDDL--ISESGVLPCLSVFCIGSHCNLPSMDK 304
>LEU2_BUCAP (O85065) 3-isopropylmalate dehydratase large subunit (EC
4.2.1.33) (Isopropylmalate isomerase) (Alpha-IPM
isomerase) (IPMI)
Length = 469
Score = 30.4 bits (67), Expect = 4.7
Identities = 21/62 (33%), Positives = 35/62 (55%), Gaps = 12/62 (19%)
Query: 180 INASTTKAIRSPKSELESIVRNAYRYQNASHDVEDPPQRTVKCHV---EEGIT---KTII 233
INAS + A K +++ +++N + A +D+ +P Q V HV E+G+T TI+
Sbjct: 72 INASGSMA----KIQMQQLIKNCEEFNIALYDLNNPKQGIV--HVIGPEQGLTLPGSTIV 125
Query: 234 CG 235
CG
Sbjct: 126 CG 127
>UVRC_NEIMB (Q9JZ26) UvrABC system protein C (UvrC protein)
(Excinuclease ABC subunit C)
Length = 617
Score = 30.0 bits (66), Expect = 6.1
Identities = 17/49 (34%), Positives = 25/49 (50%)
Query: 65 IHQMLNKLASGTRTFSDVAKLILPFTQKKVGIPIKSSKLHLTDDKLDEA 113
+H L +A G + + +LILPFT + +P S LHL DE+
Sbjct: 497 LHIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDES 545
>UVRC_NEIMA (Q9JU21) UvrABC system protein C (UvrC protein)
(Excinuclease ABC subunit C)
Length = 617
Score = 30.0 bits (66), Expect = 6.1
Identities = 17/49 (34%), Positives = 25/49 (50%)
Query: 65 IHQMLNKLASGTRTFSDVAKLILPFTQKKVGIPIKSSKLHLTDDKLDEA 113
+H L +A G + + +LILPFT + +P S LHL DE+
Sbjct: 497 LHIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDES 545
>UL71_HHV6Z (P52546) Protein U44
Length = 231
Score = 30.0 bits (66), Expect = 6.1
Identities = 25/90 (27%), Positives = 42/90 (45%), Gaps = 8/90 (8%)
Query: 38 LRTESQLLSTFDYAW--NTISNGTRRPDDIHQMLNKLASGTRTFSDVAKLILPFTQKKVG 95
L ++L T D + I + D+++Q+LNK A+ T SDV K+ +
Sbjct: 108 LMKAEKILQTMDVVFMKTLIGEFSMCTDNLNQLLNKFATDQSTLSDVEKI-----NSLIE 162
Query: 96 IPIKSSKLHLTD-DKLDEADSGSYNVLSRV 124
I ++SK L + D + ++G Y L V
Sbjct: 163 IDGENSKRLLVELDPILHEETGLYQALPNV 192
>LEU2_BUCRP (P48573) 3-isopropylmalate dehydratase large subunit (EC
4.2.1.33) (Isopropylmalate isomerase) (Alpha-IPM
isomerase) (IPMI)
Length = 471
Score = 30.0 bits (66), Expect = 6.1
Identities = 21/62 (33%), Positives = 36/62 (57%), Gaps = 12/62 (19%)
Query: 180 INASTTKAIRSPKSELESIVRNAYRYQNASHDVEDPPQRTVKCHV---EEGIT---KTII 233
INAS + A K +++ ++RN ++ + +D+ +P Q V HV E+G+T TI+
Sbjct: 72 INASGSMA----KIQMQQLIRNCNQFNISLYDLNNPKQGIV--HVIGPEQGMTLPGMTIV 125
Query: 234 CG 235
CG
Sbjct: 126 CG 127
>ICA1_MOUSE (P13597) Intercellular adhesion molecule-1 precursor
(MALA-2)
Length = 537
Score = 30.0 bits (66), Expect = 6.1
Identities = 23/84 (27%), Positives = 40/84 (47%), Gaps = 9/84 (10%)
Query: 172 CPEHKG-MRINASTTKAIRSPKSELESIVRNAYRYQNASHDVEDPPQRTVKCHVEEGITK 230
C E+ G ++ +AS T + S +E +R +Q D+ T++CHV+ G +
Sbjct: 91 CFENCGTVQSSASATITVYSFPESVE--LRPLPAWQQVGKDL------TLRCHVDGGAPR 142
Query: 231 TIICGIILDDGSTCRRQPVKGRKR 254
T + ++L RQPV G +
Sbjct: 143 TQLSAVLLRGEEILSRQPVGGHPK 166
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.133 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,697,514
Number of Sequences: 164201
Number of extensions: 1215679
Number of successful extensions: 2694
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 2687
Number of HSP's gapped (non-prelim): 16
length of query: 266
length of database: 59,974,054
effective HSP length: 108
effective length of query: 158
effective length of database: 42,240,346
effective search space: 6673974668
effective search space used: 6673974668
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 65 (29.6 bits)
Medicago: description of AC147961.4