BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147961.2 - phase: 0 /pseudo
(451 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ERG3_YEAST (P32353) C-5 sterol desaturase (EC 1.3.3.-) (Sterol-C... 34 0.88
CYPD_BACSU (O08394) Probable bifunctional P-450:NADPH-P450 reduc... 34 0.88
HEMA_IADA2 (P03446) Hemagglutinin precursor [Contains: Hemagglut... 33 1.1
DNLI_PYRFU (P56709) Thermostable DNA ligase (EC 6.5.1.1) (Polyde... 33 1.1
ERG3_LEPMC (Q8J207) C-5 sterol desaturase (EC 1.3.3.-) (Sterol-C... 32 3.3
FCP1_YEAST (Q03254) RNA polymerase II subunit A C-terminal domai... 32 4.4
ERG3_CANGA (P50860) C-5 sterol desaturase (EC 1.3.3.-) (Sterol-C... 32 4.4
SYM_SULSO (Q9UWW2) Methionyl-tRNA synthetase (EC 6.1.1.10) (Meth... 31 7.4
GEM4_HUMAN (P57678) Component of gems 4 (Gemin4) (p97) 31 7.4
DP3A_RICPR (O05974) DNA polymerase III alpha subunit (EC 2.7.7.7) 31 7.4
THYX_AERPE (Q9YA75) Thymidylate synthase thyX (EC 2.1.1.148) (TS... 30 9.7
SC5D_TOBAC (Q9ZT29) Delta-7-sterol-C5(6)-desaturase (EC 1.3.3.-)... 30 9.7
PTA_BUCBP (Q89AS7) Phosphate acetyltransferase (EC 2.3.1.8) (Pho... 30 9.7
HM40_CAEEL (Q19503) Homeobox protein ceh-40 30 9.7
ERG3_ASHGO (Q754B9) C-5 sterol desaturase (EC 1.3.3.-) (Sterol-C... 30 9.7
>ERG3_YEAST (P32353) C-5 sterol desaturase (EC 1.3.3.-)
(Sterol-C5-desaturase) (Ergosterol delta 5,6 desaturase)
Length = 365
Score = 33.9 bits (76), Expect = 0.88
Identities = 14/46 (30%), Positives = 25/46 (53%)
Query: 80 HSLHHTKFRRNYSLFMPMYDYIYGTVDKSTDVIYETSLMRPKESPD 125
H++HH F NY F ++D + G+ + D +++ L KE+ D
Sbjct: 288 HTVHHLYFNYNYGQFTTLWDRLGGSYRRPDDSLFDPKLRDAKETWD 333
>CYPD_BACSU (O08394) Probable bifunctional P-450:NADPH-P450
reductase 1 [Includes: Cytochrome P450 102 (EC
1.14.14.1); NADPH--cytochrome P450 reductase (EC
1.6.2.4)]
Length = 1061
Score = 33.9 bits (76), Expect = 0.88
Identities = 39/153 (25%), Positives = 68/153 (43%), Gaps = 24/153 (15%)
Query: 83 HHTKFRRNYSLFMP------MYDYIYGTVDKSTDVIYETSLMRPKESPDVV-HLTHLTTF 135
H +R+ +++ MP M DY VD + +I + + + P E+ DV +T LT
Sbjct: 94 HEPNWRKAHNILMPTFSQRAMKDYHEKMVDIAVQLIQKWARLNPNEAVDVPGDMTRLTLD 153
Query: 136 NSIYQLRLGFASLASNPQTSKWYLHLMWPFTMFSMLMTWICGRAFVLESNSFKNLKLQCW 195
+G N + + +Y PF + SM+ RA + + L +Q
Sbjct: 154 T------IGLCGF--NYRFNSYYRETPHPF-INSMV------RALDEAMHQMQRLDVQDK 198
Query: 196 LIPRFKRQYFSKWQSKTFNNLIEEAIVEAELNG 228
L+ R KRQ+ ++ +T +L++ I E NG
Sbjct: 199 LMVRTKRQF--RYDIQTMFSLVDSIIAERRANG 229
>HEMA_IADA2 (P03446) Hemagglutinin precursor [Contains:
Hemagglutinin HA1 chain; Hemagglutinin HA2 chain]
Length = 564
Score = 33.5 bits (75), Expect = 1.1
Identities = 26/81 (32%), Positives = 37/81 (45%), Gaps = 15/81 (18%)
Query: 214 NNL-IEEAIVEAELNGAKVISLGLFNKNHQLNERHEHYIGRLPQLKIKVVDGSSLAAATV 272
NNL + + + E +LN G+ N + +HYIG+ P K + SL A
Sbjct: 283 NNLPMGQCVTECQLNE------GVMNTSKPFQNTSKHYIGKCP----KYIPSGSLKLAIG 332
Query: 273 LNNIPKGTNQVLLRGKFNKVA 293
L N+P QV RG F +A
Sbjct: 333 LRNVP----QVQDRGLFGAIA 349
>DNLI_PYRFU (P56709) Thermostable DNA ligase (EC 6.5.1.1)
(Polydeoxyribonucleotide synthase [ATP]) (Pfu DNA
ligase)
Length = 561
Score = 33.5 bits (75), Expect = 1.1
Identities = 39/152 (25%), Positives = 59/152 (38%), Gaps = 25/152 (16%)
Query: 218 EEAIVEAEL-----NGAKV---ISLGLFNKNHQLNERHEHYIGRLPQLKIKVVDGSSLAA 269
E+AIVE EL NG + L F + H + E E L + VDG SL
Sbjct: 292 EKAIVEGELVAIGENGRPLPFQYVLRRFRRKHNIEEMMEKIPLELNLFDVLYVDGQSLID 351
Query: 270 ATVLNNIPKGTNQVLLRGKFNKVAFVIANALCKKNVQVVVLYKDELKELEQRINTSKGNL 329
++ + T + ++ K N+ V N + KK + YK L+ + + + +
Sbjct: 352 TKFIDR--RRTLEEII--KQNEKIKVAENLITKKVEEAEAFYKRALEMGHEGLMAKRLDA 407
Query: 330 ALSPFNTPKIWL-------------VGDEWDE 348
P N K WL +G EW E
Sbjct: 408 VYEPGNRGKKWLKIKPTMENLDLVIIGAEWGE 439
>ERG3_LEPMC (Q8J207) C-5 sterol desaturase (EC 1.3.3.-)
(Sterol-C5-desaturase) (Ergosterol delta 5,6 desaturase)
Length = 356
Score = 32.0 bits (71), Expect = 3.3
Identities = 12/38 (31%), Positives = 21/38 (54%)
Query: 80 HSLHHTKFRRNYSLFMPMYDYIYGTVDKSTDVIYETSL 117
H++HH F NY F ++D + G+ K D +++ L
Sbjct: 280 HTMHHLYFNYNYGQFTTLWDRLGGSYRKPNDELFKREL 317
>FCP1_YEAST (Q03254) RNA polymerase II subunit A C-terminal domain
phosphatase (EC 3.1.3.16) (CTD phosphatase FCP1)
Length = 732
Score = 31.6 bits (70), Expect = 4.4
Identities = 29/96 (30%), Positives = 46/96 (47%), Gaps = 12/96 (12%)
Query: 81 SLHHTKFRRNYSLFMPMYDYIYGTVDKSTDVIYETSLMRPKESPDVVHL-THLTTFN-SI 138
SL F+ Y +F + GT + +D++ TS +PD+ +L THL T N S
Sbjct: 498 SLKQKVFQNCYFVFSGLIPL--GTDIQRSDIVIWTSTFGATSTPDIDYLTTHLITKNPST 555
Query: 139 YQLRLGFASLASNPQTSKWYLHLMWPFTMFSMLMTW 174
Y+ RL + NPQ + ++ P +F L+ W
Sbjct: 556 YKARL---AKKFNPQ-----IKIVHPDWIFECLVNW 583
>ERG3_CANGA (P50860) C-5 sterol desaturase (EC 1.3.3.-)
(Sterol-C5-desaturase) (Ergosterol delta 5,6 desaturase)
Length = 364
Score = 31.6 bits (70), Expect = 4.4
Identities = 12/43 (27%), Positives = 23/43 (52%)
Query: 80 HSLHHTKFRRNYSLFMPMYDYIYGTVDKSTDVIYETSLMRPKE 122
H++HH F NY F ++D + G+ + D +++ L K+
Sbjct: 289 HTVHHLYFNYNYGQFTTLWDRLGGSYRRPEDSLFDPKLKMDKK 331
>SYM_SULSO (Q9UWW2) Methionyl-tRNA synthetase (EC 6.1.1.10)
(Methionine--tRNA ligase) (MetRS)
Length = 573
Score = 30.8 bits (68), Expect = 7.4
Identities = 19/68 (27%), Positives = 34/68 (49%), Gaps = 5/68 (7%)
Query: 221 IVEAELNGAKVISLGLFNKNHQLNERHEHYIGRLPQLKIKVVDGSSLAAATVLNNIPKGT 280
IV ELN +G + N L+ + +Y G +P+ KI ++D + +++N PK
Sbjct: 380 IVNTELND----DIGNY-VNRVLSMVNRYYSGIVPEFKIDILDDNDRKIISLINETPKVV 434
Query: 281 NQVLLRGK 288
+ +GK
Sbjct: 435 GDLFEKGK 442
>GEM4_HUMAN (P57678) Component of gems 4 (Gemin4) (p97)
Length = 1058
Score = 30.8 bits (68), Expect = 7.4
Identities = 26/90 (28%), Positives = 33/90 (35%), Gaps = 24/90 (26%)
Query: 340 WLVGDEWDEYEQMEA---PKGSLFIPFS----HFPPKKMRKDCFYHYTPAMITPTTF--M 390
W EW Q+ K L +P+S F P K +++ TF +
Sbjct: 863 WCSPQEWQRLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQLSVLFLRTFQFL 922
Query: 391 NSHSCENWLPRRVMSAWRIAGIIHALEGWN 420
SHSC NWLP LEGWN
Sbjct: 923 CSHSCRNWLP---------------LEGWN 937
>DP3A_RICPR (O05974) DNA polymerase III alpha subunit (EC 2.7.7.7)
Length = 1182
Score = 30.8 bits (68), Expect = 7.4
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 11/59 (18%)
Query: 233 SLGLFNKNHQLNERHEHYIGRLPQLKIKVVDGSSLAAATVLNNIPKGTNQVLLRGKFNK 291
++GLF NH L E Y G +L I L + N +P GTN+V L G K
Sbjct: 987 AMGLFLSNHPLTE----YQGIFSRLNI-------LNTRDLYNKLPNGTNRVTLAGVIQK 1034
>THYX_AERPE (Q9YA75) Thymidylate synthase thyX (EC 2.1.1.148) (TS)
(TSase)
Length = 327
Score = 30.4 bits (67), Expect = 9.7
Identities = 8/28 (28%), Positives = 19/28 (67%)
Query: 191 KLQCWLIPRFKRQYFSKWQSKTFNNLIE 218
+++ W++ F+RQ+FS W+ + ++E
Sbjct: 52 EVETWILETFRRQHFSPWEHSVYTFMVE 79
>SC5D_TOBAC (Q9ZT29) Delta-7-sterol-C5(6)-desaturase (EC 1.3.3.-)
(Delta-7-C-5 sterol desaturase)
(Delta7-sterol-C5-desaturase)
Length = 271
Score = 30.4 bits (67), Expect = 9.7
Identities = 9/27 (33%), Positives = 18/27 (66%)
Query: 79 FHSLHHTKFRRNYSLFMPMYDYIYGTV 105
+H++HH +R NY + D+++GT+
Sbjct: 234 YHTIHHRTYRHNYGHYTIWMDWMFGTL 260
>PTA_BUCBP (Q89AS7) Phosphate acetyltransferase (EC 2.3.1.8)
(Phosphotransacetylase)
Length = 715
Score = 30.4 bits (67), Expect = 9.7
Identities = 24/92 (26%), Positives = 42/92 (45%), Gaps = 8/92 (8%)
Query: 235 GLFNKNHQ--LNERHEHYIGRLPQLKIKVVDGSSLAAATVLNNIPKGTNQVLLRGKFNKV 292
G K+H L+ +YI + ++I V ++ +NNI N V + K +
Sbjct: 108 GFDTKSHTYVLSNDINYYISKSINVEIVFVCAVDRSSQLDINNIVYIINNVFKKNKNINI 167
Query: 293 AFVIANALCKK------NVQVVVLYKDELKEL 318
VI N LC+K NV + ++K +K++
Sbjct: 168 RGVIINELCQKVRNDYGNVNLFDMFKSSVKKI 199
>HM40_CAEEL (Q19503) Homeobox protein ceh-40
Length = 329
Score = 30.4 bits (67), Expect = 9.7
Identities = 14/41 (34%), Positives = 22/41 (53%)
Query: 285 LRGKFNKVAFVIANALCKKNVQVVVLYKDELKELEQRINTS 325
+ GKFNKV FV+ C++ +Q+ Y D ++ TS
Sbjct: 158 MSGKFNKVCFVLKQTACEEVIQLKKRYLDARRKRRNFSKTS 198
>ERG3_ASHGO (Q754B9) C-5 sterol desaturase (EC 1.3.3.-)
(Sterol-C5-desaturase) (Ergosterol delta 5,6 desaturase)
Length = 351
Score = 30.4 bits (67), Expect = 9.7
Identities = 11/42 (26%), Positives = 24/42 (56%)
Query: 80 HSLHHTKFRRNYSLFMPMYDYIYGTVDKSTDVIYETSLMRPK 121
H++HH F NY F ++D + G+ + +++++L + K
Sbjct: 282 HTVHHLYFNYNYGQFTTLWDRLGGSYREPDHELFDSNLKKDK 323
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.328 0.139 0.455
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,812,384
Number of Sequences: 164201
Number of extensions: 2260397
Number of successful extensions: 6085
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 6077
Number of HSP's gapped (non-prelim): 15
length of query: 451
length of database: 59,974,054
effective HSP length: 114
effective length of query: 337
effective length of database: 41,255,140
effective search space: 13902982180
effective search space used: 13902982180
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 67 (30.4 bits)
Medicago: description of AC147961.2