BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147960.7 + phase: 0
(248 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SLA2_BACAN (P94217) S-layer protein EA1 precursor 38 0.020
APL3_HUMAN (O95236) Apolipoprotein L3 (Apolipoprotein L-III) (Ap... 33 0.84
G6PI_DEIRA (Q9RTL8) Glucose-6-phosphate isomerase (EC 5.3.1.9) (... 32 1.4
CYPE_BACSU (O08336) Probable bifunctional P-450:NADPH-P450 reduc... 32 1.9
VN34_ROTS4 (Q00721) Nonstructural RNA-binding protein 34 (NS34) ... 31 2.4
VN34_ROTS1 (P03536) Nonstructural RNA-binding protein 34 (NS34) ... 31 3.2
THIL_RHIME (P50174) Acetyl-CoA acetyltransferase (EC 2.3.1.9) (A... 31 3.2
HNMT_RAT (Q01984) Histamine N-methyltransferase (EC 2.1.1.8) (HMT) 31 3.2
FIXG_RHIME (P18396) Nitrogen fixation protein fixG 30 4.2
MXIK_SHIFL (Q06082) MxiK protein 30 5.4
FOS_CYPCA (P79702) Proto-oncogene protein c-fos (Cellular oncoge... 30 5.4
VATD_DEIRA (Q9RWG6) V-type ATP synthase subunit D (EC 3.6.3.14) ... 30 7.1
TRPE_CITFR (P00896) Anthranilate synthase component I (EC 4.1.3.... 30 7.1
PLSX_GLOVI (Q7NMG6) Fatty acid/phospholipid synthesis protein plsX 30 7.1
CH60_LACPL (Q88YM5) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 30 7.1
UGA3_YEAST (P26370) Transcriptional activator protein UGA3 29 9.3
PIF1_YEAST (P07271) DNA repair and recombination protein PIF1, m... 29 9.3
GPB2_YEAST (P39717) Guanine nucleotide-binding protein beta subu... 29 9.3
>SLA2_BACAN (P94217) S-layer protein EA1 precursor
Length = 862
Score = 38.1 bits (87), Expect = 0.020
Identities = 37/126 (29%), Positives = 54/126 (42%), Gaps = 8/126 (6%)
Query: 103 AFVSLPQVTFGARRWELPEAKHGVSASTANELRQDRYD---VNVNPEKLKAASEGLANLG 159
AF + V+ + + P+ K ++ ST E + +Y V +PE L EG
Sbjct: 616 AFKNFELVSKVGQYGQSPDTKLDLNVSTTVEYQLSKYTSDRVYSDPENL----EGYEVES 671
Query: 160 KAFAIATAVVFGGAAMVIGMVASKLELHNMGDLKTKGKDVVEPQLENIKNYFVPMK-VWA 218
K A+A A + G +V G K+++H + T GK VE E I V K V
Sbjct: 672 KNLAVADAKIVGNKVVVTGKTPGKVDIHLTKNGATAGKATVEIVQETIAIKSVNFKPVQT 731
Query: 219 ENMSRK 224
EN K
Sbjct: 732 ENFVEK 737
>APL3_HUMAN (O95236) Apolipoprotein L3 (Apolipoprotein L-III)
(ApoL-III) (TNF-inducible protein CG12-1) (CG12_1)
Length = 402
Score = 32.7 bits (73), Expect = 0.84
Identities = 45/215 (20%), Positives = 78/215 (35%), Gaps = 6/215 (2%)
Query: 2 SFLAGRLAGKEAAYFFQESKQAVTKLAQKNNPISKTNVVDQRHVVQDNADVLPEVLRHSL 61
SFL + +EA +F+E V N + V + +D AD L E L+
Sbjct: 72 SFLEKKRFTEEATKYFRERVSPVHLQILLTNNEAWKRFVTAAELPRDEADALYEALKKLR 131
Query: 62 PSKLFRDETASSSSFSASKWVLQSDPKLRSSVSPDAINPLRAFVS-LPQVTFGARRWELP 120
DE +W L+ P+++ + ++I LRA + + +V G +
Sbjct: 132 TYAAIEDEYVQQKDEQFREWFLKEFPQVKRKIQ-ESIEKLRALANGIEEVHRGCTISNVV 190
Query: 121 EAKHGVSASTANELRQDRYDVNVNPE-KLKAASEGLANLGKAFAIATAVVFGGAAMVIGM 179
+ G ++ + L AA GL I T++V
Sbjct: 191 SSSTGAASGIMSLAGLVLAPFTAGTSLALTAAGVGLGAASAVTGITTSIVEHSYTSSAEA 250
Query: 180 VASKLELHNMGDLKT---KGKDVVEPQLENIKNYF 211
AS+L ++ LK +D+ L + NY+
Sbjct: 251 EASRLTATSIDRLKVFKEVMRDITPNLLSLLNNYY 285
>G6PI_DEIRA (Q9RTL8) Glucose-6-phosphate isomerase (EC 5.3.1.9)
(GPI) (Phosphoglucose isomerase) (PGI) (Phosphohexose
isomerase) (PHI)
Length = 541
Score = 32.0 bits (71), Expect = 1.4
Identities = 18/64 (28%), Positives = 33/64 (51%)
Query: 22 QAVTKLAQKNNPISKTNVVDQRHVVQDNADVLPEVLRHSLPSKLFRDETASSSSFSASKW 81
+A+ AQ++ + + VD + + D+ PEV + SK F + +++ SA KW
Sbjct: 164 EALKHYAQRDLTVRFVSNVDGTDLTEKTRDLDPEVTLFIVSSKTFTTQETMTNARSARKW 223
Query: 82 VLQS 85
+L S
Sbjct: 224 LLGS 227
>CYPE_BACSU (O08336) Probable bifunctional P-450:NADPH-P450
reductase 2 [Includes: Cytochrome P450 102 (EC
1.14.14.1); NADPH--cytochrome P450 reductase (EC
1.6.2.4)]
Length = 1054
Score = 31.6 bits (70), Expect = 1.9
Identities = 27/110 (24%), Positives = 48/110 (43%), Gaps = 6/110 (5%)
Query: 131 ANELRQDRYDVNVNPEKLKAASEGLANLGKAFAIATAVVFGGAAMVIGMVASKLELHNMG 190
A + R +R++ +P + + G+ I A MV+G+V EL N
Sbjct: 374 AEDFRPERFE---DPSSIPHHAYKPFGNGQRACIGMQFALQEATMVLGLVLKHFELINHT 430
Query: 191 DLKTKGKDVVEPQLENIKNYFVPMKVWAENMSRKWHLEREDVKQKAIVKD 240
+ K K+ + + ++ K P K A N+ RK E+ D+K + K+
Sbjct: 431 GYELKIKEALTIKPDDFKITVKPRKTAAINVQRK---EQADIKAETKPKE 477
>VN34_ROTS4 (Q00721) Nonstructural RNA-binding protein 34 (NS34)
(NCVP4)
Length = 315
Score = 31.2 bits (69), Expect = 2.4
Identities = 27/87 (31%), Positives = 39/87 (44%), Gaps = 15/87 (17%)
Query: 32 NPISKTNVVDQRHVVQDNADVLPEVLRHSLPSKLFRDETASSSSFSASKWVLQS---DPK 88
N S NV+ Q+ V L L +K+ S S+ +W L+S DP+
Sbjct: 205 NMHSLQNVISQQQAHIAELQVYNNKLERDLQNKI-------GSLTSSIEWYLRSMELDPE 257
Query: 89 LRSSV-----SPDAINPLRAFVSLPQV 110
+++ + S DAINPL AF L V
Sbjct: 258 IKADIEQQINSIDAINPLHAFDDLESV 284
>VN34_ROTS1 (P03536) Nonstructural RNA-binding protein 34 (NS34)
(NCVP4)
Length = 315
Score = 30.8 bits (68), Expect = 3.2
Identities = 27/87 (31%), Positives = 39/87 (44%), Gaps = 15/87 (17%)
Query: 32 NPISKTNVVDQRHVVQDNADVLPEVLRHSLPSKLFRDETASSSSFSASKWVLQS---DPK 88
N S NV+ Q+ V L L +K+ S S+ +W L+S DP+
Sbjct: 205 NMHSLQNVIPQQQAHIAELQVYNNKLERDLQNKI-------GSLTSSIEWYLRSMELDPE 257
Query: 89 LRSSV-----SPDAINPLRAFVSLPQV 110
+++ + S DAINPL AF L V
Sbjct: 258 IKADIEQQINSIDAINPLHAFDDLESV 284
>THIL_RHIME (P50174) Acetyl-CoA acetyltransferase (EC 2.3.1.9)
(Acetoacetyl-CoA thiolase)
Length = 393
Score = 30.8 bits (68), Expect = 3.2
Identities = 32/118 (27%), Positives = 47/118 (39%), Gaps = 14/118 (11%)
Query: 132 NELRQDRYDVNVNPEKLKAASEGLANLG-KAFAIATAVVFGGAAMVI---GMVASKLELH 187
N RQ + EK L G +A A+ + G A VI GM + + H
Sbjct: 66 NPARQAAMKAGLPQEKTAWGMNQLCGSGLRAVALGMQQIATGDAKVIVAGGMESMSMAPH 125
Query: 188 --------NMGDLKTKGKDVVEPQLENIKNYFVPMKVWAENMSRKWHLEREDVKQKAI 237
MGD K + + + Y M + AEN++RKW L RE+ + A+
Sbjct: 126 CAHLRGGVKMGDYKMIDTMIKDGLTDAFYGYH--MGITAENVARKWQLTREEQDEFAL 181
>HNMT_RAT (Q01984) Histamine N-methyltransferase (EC 2.1.1.8) (HMT)
Length = 294
Score = 30.8 bits (68), Expect = 3.2
Identities = 26/89 (29%), Positives = 39/89 (43%), Gaps = 5/89 (5%)
Query: 155 LANLGKAFAIATAVVFGGAAMVIGM-VASKLELHNMGDLKTKGKDVVEPQLENIKNY--F 211
+A +G+ A + GG A I + + SK++ G +VVEP E I Y
Sbjct: 43 IARIGETKAEIKILSIGGGAGEIDLQILSKVQAQYPGICINN--EVVEPNAEQIVKYKEL 100
Query: 212 VPMKVWAENMSRKWHLEREDVKQKAIVKD 240
V EN+ WH E QK +V++
Sbjct: 101 VAKTSNMENIKFAWHKETSSEYQKRVVEE 129
>FIXG_RHIME (P18396) Nitrogen fixation protein fixG
Length = 524
Score = 30.4 bits (67), Expect = 4.2
Identities = 19/54 (35%), Positives = 26/54 (47%), Gaps = 9/54 (16%)
Query: 93 VSPDAINPLRAFVSLPQVTFGARRWELPEAKHGVS--ASTANELRQDRYDVNVN 144
V PD + PL+ FV+LP+ F EA+ G S A + +D Y N N
Sbjct: 471 VEPDKVTPLKVFVTLPKGRFA-------EAEEGFSLIAEDPSSHERDVYQANFN 517
>MXIK_SHIFL (Q06082) MxiK protein
Length = 175
Score = 30.0 bits (66), Expect = 5.4
Identities = 29/117 (24%), Positives = 47/117 (39%), Gaps = 10/117 (8%)
Query: 108 PQVTFGARRWELPEA--KHGVSASTANELRQDRYDVNVNPEKLKAASEGLANLGKAFAIA 165
P R LP ++GV S N L ++YD+N + E L + A +A
Sbjct: 18 PAFYINRNRLNLPSELLENGVIRSEINNLIINKYDLNCDIEPLSGVT--------AMFVA 69
Query: 166 TAVVFGGAAMVIGMVASKLELHNMGDLKTKGKDVVEPQLENIKNYFVPMKVWAENMS 222
+ A IG S+L H+ + G + + I++ F + W EN+S
Sbjct: 70 NWNLLPAVAYFIGSQESRLINHSEMVISYYGGKISKQGEAAIRSGFWHLIAWKENIS 126
>FOS_CYPCA (P79702) Proto-oncogene protein c-fos (Cellular oncogene
fos)
Length = 347
Score = 30.0 bits (66), Expect = 5.4
Identities = 30/120 (25%), Positives = 48/120 (40%), Gaps = 6/120 (5%)
Query: 4 LAGRLAGKEAAYFFQESKQAVTKLAQKN-NPISKTNVVDQRHVVQDNADVLPEVLRHSLP 62
+A L KE F + + + K+ + +PI +V + + + S
Sbjct: 159 IANLLKEKERLEFILAAHKPICKIPSSSVSPIPAASVPEIHSITTSVVSTANAPVTTSSS 218
Query: 63 SKLFRDETASSSSFSASKWVLQSDPKLRSSVSPDAINPLRAFVSLPQVTFG----ARRWE 118
S LF TAS+ SF ++ + +P L S+ A L S+P V AR WE
Sbjct: 219 SSLF-SSTASTDSFGSTVEISDLEPTLEESLELLAKAELETARSVPDVDLSSSLYARDWE 277
>VATD_DEIRA (Q9RWG6) V-type ATP synthase subunit D (EC 3.6.3.14)
(V-type ATPase subunit D)
Length = 224
Score = 29.6 bits (65), Expect = 7.1
Identities = 25/90 (27%), Positives = 41/90 (44%), Gaps = 11/90 (12%)
Query: 112 FGARRWELPEAKHGVSASTANELRQDR-----YDVNVN----PEKLKAASEGLANLGKAF 162
FGA+ W+ PEA +S + + D Y V V PE+ A N+G A
Sbjct: 66 FGAKAWDSPEAVESLSLAGTGDYAVDMQIESIYGVKVPKINIPERAAQADFSPINVG-AR 124
Query: 163 AIATAVVFGGA-AMVIGMVASKLELHNMGD 191
I + FGG ++ + A++ +L +G+
Sbjct: 125 TIQASNDFGGVLEAIVKVAATETKLRRIGE 154
>TRPE_CITFR (P00896) Anthranilate synthase component I (EC 4.1.3.27)
(Fragment)
Length = 150
Score = 29.6 bits (65), Expect = 7.1
Identities = 14/33 (42%), Positives = 20/33 (60%)
Query: 76 FSASKWVLQSDPKLRSSVSPDAINPLRAFVSLP 108
F A +L D +LRS +PDA PL+ V++P
Sbjct: 105 FPAVSPLLDEDVRLRSLSAPDAFRPLQELVNVP 137
>PLSX_GLOVI (Q7NMG6) Fatty acid/phospholipid synthesis protein plsX
Length = 338
Score = 29.6 bits (65), Expect = 7.1
Identities = 37/174 (21%), Positives = 65/174 (37%), Gaps = 22/174 (12%)
Query: 52 VLPEVLRHSLPSKLFRDETASSSSFSASKWVLQSDPKLRSSVSPDAINPLRAFVSLPQVT 111
VLPE+ RH+ + + +++ P V+ D + RA ++
Sbjct: 48 VLPELRRHNAAQSVEIVDAPEQVGMGEEPTIVRRKPNSSIMVTMDLVKQGRAEAAVAAGN 107
Query: 112 FGA-------RRWELPEAKHGVSASTANELRQDR----YDVNVNPEKLKAASEGLANLGK 160
GA R LP + + L+ + DV N + E A +G
Sbjct: 108 TGAAMAAALFRIGRLPGIERPAIGAMLPTLKLGKRVLLLDVGANTDSRPRFLEQFALMGA 167
Query: 161 AFAIATAVVFGGAAMVIGMVASKLELHNMGDLKTKGKDVVEPQLEN-IKNYFVP 213
++ V+G+ K+ L N+G+ + KG ++V E +KN VP
Sbjct: 168 LYS----------RYVLGVAEPKVGLLNIGEERGKGNELVADAYEMLVKNPHVP 211
>CH60_LACPL (Q88YM5) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 541
Score = 29.6 bits (65), Expect = 7.1
Identities = 28/110 (25%), Positives = 50/110 (45%), Gaps = 19/110 (17%)
Query: 126 VSASTANELRQDRYDVNVNPEKLKAASEGLANLGKAFAIATAVVFGGAAMVIGMVASKLE 185
V A+T EL++ +Y + E N +A A+ V GG +I ++
Sbjct: 379 VGAATETELKERKYRI-----------EDALNATRA-AVEEGFVAGGGTALINVIKDVAA 426
Query: 186 LHNMGDLKTKGKDVVEPQLENIKNYFVPMKVWAENMSRKWHLEREDVKQK 235
L GD++T G ++V+ LE P++ AEN + + E +K++
Sbjct: 427 LKETGDVQT-GINIVKRALEE------PVRQIAENAGLEGSVIVEKMKEQ 469
>UGA3_YEAST (P26370) Transcriptional activator protein UGA3
Length = 528
Score = 29.3 bits (64), Expect = 9.3
Identities = 36/131 (27%), Positives = 54/131 (40%), Gaps = 27/131 (20%)
Query: 110 VTFGARRWELPEAKHGVSASTANELRQDRYDVNVNPE---------KLKAASEGLANLGK 160
+ F W A H +S S++NELR+D VN+ + K S A LG
Sbjct: 157 ILFAILAWS---ANH-LSISSSNELRKDEIFVNLANKYTYMSLSHLKTNEGSSACAKLGF 212
Query: 161 AFAIATAVVFGGAAMVIGMVASKLELHNMGDLKTK---GKDV--------VEPQLEN--- 206
+++A ++ G+ + G V L N+G + GKDV EP LE
Sbjct: 213 LYSLAQILILCGSEICQGDVKFWKILLNIGKNLIENHVGKDVSRILTTTTEEPSLEERII 272
Query: 207 IKNYFVPMKVW 217
N+ +K W
Sbjct: 273 FPNFNSVVKYW 283
>PIF1_YEAST (P07271) DNA repair and recombination protein PIF1,
mitochondrial precursor
Length = 857
Score = 29.3 bits (64), Expect = 9.3
Identities = 30/87 (34%), Positives = 42/87 (47%), Gaps = 4/87 (4%)
Query: 144 NPEKLKAASEGLANLGKAFAIA---TAVVFGGAAMVIGMVASKLELHNMGDLKTKGKDVV 200
NP KLKAA E + G+ A+A ++V G A IG S L+ ++ D + K
Sbjct: 571 NPSKLKAAMEREQSDGEESAVASRKSSVKEGFAKSDIGEPVSPLD-SSVFDFMKRVKTDD 629
Query: 201 EPQLENIKNYFVPMKVWAENMSRKWHL 227
E LENIK M+ +N + K L
Sbjct: 630 EVVLENIKRKEQLMQTIHQNSAGKRRL 656
>GPB2_YEAST (P39717) Guanine nucleotide-binding protein beta subunit
GPB2 (Gbeta mimic Kelch protein)
Length = 880
Score = 29.3 bits (64), Expect = 9.3
Identities = 27/99 (27%), Positives = 41/99 (41%), Gaps = 7/99 (7%)
Query: 30 KNNPISKTNVVDQRHVVQDNADVLPEVLRHSLPSKLFRDETASSSSFSA-----SKWVLQ 84
KN+ + K + + Q VV V P ++ H LF +A+ S A S
Sbjct: 590 KNHFVLKPSYISQDRVVSPKP-VFPMMV-HGTHQDLFNSGSAAQESPKAGASASSASAAS 647
Query: 85 SDPKLRSSVSPDAINPLRAFVSLPQVTFGARRWELPEAK 123
DP + ++ INP R S+P G +R L + K
Sbjct: 648 FDPDMDDNLENYIINPGRKSSSIPMTAIGRQRLILSQEK 686
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.314 0.129 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,135,373
Number of Sequences: 164201
Number of extensions: 1042191
Number of successful extensions: 2408
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 2403
Number of HSP's gapped (non-prelim): 19
length of query: 248
length of database: 59,974,054
effective HSP length: 107
effective length of query: 141
effective length of database: 42,404,547
effective search space: 5979041127
effective search space used: 5979041127
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 64 (29.3 bits)
Medicago: description of AC147960.7