BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147714.14 + phase: 0
(129 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contain... 52 2e-07
YCH4_YEAST (P25600) Transposon Ty5-1 34.5 kDa hypothetical protein 47 1e-05
POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran... 36 0.013
M810_ARATH (P92519) Hypothetical mitochondrial protein AtMg00810... 36 0.017
TIG_RHOBA (Q7URG5) Trigger factor (TF) 30 1.2
SKIL_HUMAN (P12757) Ski-like protein SKIL (Ski-related oncogene) 28 2.7
DNAK_BUCBP (P59565) Chaperone protein dnaK (Heat shock protein 7... 28 2.7
TERT_SCHPO (O13339) Telomerase reverse transcriptase (EC 2.7.7.-... 28 3.6
SKIL_MOUSE (Q60665) Ski-related oncogene SNO isoform SNON1/DE3 28 3.6
RPOB_XYLFT (Q87A32) DNA-directed RNA polymerase beta chain (EC 2... 28 3.6
RPOB_XYLFA (Q9PA86) DNA-directed RNA polymerase beta chain (EC 2... 28 3.6
PMC4_SCHPO (Q9Y821) RNA polymerase II mediator complex protein pmc4 28 3.6
ETR1_PELHO (Q9XH58) Ethylene receptor 1 (EC 2.7.3.-) (PhETR1) 28 4.6
ETR1_LYCES (Q41342) Ethylene receptor 1 (EC 2.7.3.-) (LeETR1) 28 4.6
DHAM_MOUSE (P47738) Aldehyde dehydrogenase, mitochondrial precur... 28 4.6
NFIB_CHICK (P17924) Nuclear factor 1 B-type (Nuclear factor 1/B)... 27 6.1
VGLB_VZVD (P09257) Glycoprotein B precursor (Glycoprotein II) 27 7.9
RPOA_VIBVY (Q7MPG4) DNA-directed RNA polymerase alpha chain (EC ... 27 7.9
RPOA_VIBVU (Q8DE63) DNA-directed RNA polymerase alpha chain (EC ... 27 7.9
RPOA_VIBPA (Q87SZ0) DNA-directed RNA polymerase alpha chain (EC ... 27 7.9
>COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contains:
Copia VLP protein; Copia protease (EC 3.4.23.-)]
Length = 1409
Score = 52.4 bits (124), Expect = 2e-07
Identities = 28/96 (29%), Positives = 51/96 (52%), Gaps = 6/96 (6%)
Query: 36 DEIEMSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCT-- 93
++ M+ + E+K F+ I+I +D++Y+ QS Y K +L KF +++CN ++TP+
Sbjct: 1110 EKFRMTDLNEIKHFIGIRIEMQEDKIYLSQSAYVKKILSKFNMENCNAVSTPLPSKINYE 1169
Query: 94 LSEEDADAKVDQKLFRGIILFDDSILFLILRPILIT 129
L D D + G +++ + L RP L T
Sbjct: 1170 LLNSDEDCNTPCRSLIGCLMY----IMLCTRPDLTT 1201
Score = 33.5 bits (75), Expect = 0.085
Identities = 13/23 (56%), Positives = 18/23 (77%)
Query: 1 MKVKSAFLNGAISEEVYVKQPPG 23
M VK+AFLNG + EE+Y++ P G
Sbjct: 1001 MDVKTAFLNGTLKEEIYMRLPQG 1023
>YCH4_YEAST (P25600) Transposon Ty5-1 34.5 kDa hypothetical
protein
Length = 308
Score = 46.6 bits (109), Expect = 1e-05
Identities = 19/35 (54%), Positives = 27/35 (76%)
Query: 1 MKVKSAFLNGAISEEVYVKQPPGFEDLKHPDYVYK 35
M V +AFLN + E +YVKQPPGF + ++PDYV++
Sbjct: 1 MDVDTAFLNSTMDEPIYVKQPPGFVNERNPDYVWE 35
>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from
transposon TNT 1-94 [Contains: Protease (EC 3.4.23.-);
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1328
Score = 36.2 bits (82), Expect = 0.013
Identities = 14/25 (56%), Positives = 21/25 (84%)
Query: 1 MKVKSAFLNGAISEEVYVKQPPGFE 25
+ VK+AFL+G + EE+Y++QP GFE
Sbjct: 921 LDVKTAFLHGDLEEEIYMEQPEGFE 945
>M810_ARATH (P92519) Hypothetical mitochondrial protein AtMg00810
(ORF240b)
Length = 240
Score = 35.8 bits (81), Expect = 0.017
Identities = 22/73 (30%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Query: 40 MSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTLSEEDA 99
M +G + +FL IQI +++ Q+KY + +L + DC M+TP+ P S
Sbjct: 33 MKDLGPVHYFLGIQIKTHPSGLFLSQTKYAEQILNNAGMLDCKPMSTPL-PLKLNSSVST 91
Query: 100 DAKVDQKLFRGII 112
D FR I+
Sbjct: 92 AKYPDPSDFRSIV 104
>TIG_RHOBA (Q7URG5) Trigger factor (TF)
Length = 480
Score = 29.6 bits (65), Expect = 1.2
Identities = 26/108 (24%), Positives = 47/108 (43%), Gaps = 23/108 (21%)
Query: 2 KVKSAFLNGAISEEVYVKQPPGFEDLKHPDYVYKDEIEMSMMGELKFFLRIQINQCKDEV 61
+VK + L ++++ V + F + PD+ Y + IE+ GE K+ I++
Sbjct: 63 RVKGSLLMDSLAK---VTEDAEFSAIGEPDFDY-ESIELPEKGEFKYQFSIEVRP----- 113
Query: 62 YVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTLSEEDADAKVDQKLFR 109
+F+ D ++ P T+SEED DA + + L R
Sbjct: 114 -------------EFETPDWKKLELKK-PVETISEEDVDAALQRVLSR 147
>SKIL_HUMAN (P12757) Ski-like protein SKIL (Ski-related oncogene)
Length = 684
Score = 28.5 bits (62), Expect = 2.7
Identities = 18/51 (35%), Positives = 25/51 (48%)
Query: 46 LKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTLSE 96
L+ F QIN DE+Y++ S+ T D L KV N P TL++
Sbjct: 175 LREFTLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTD 225
>DNAK_BUCBP (P59565) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 638
Score = 28.5 bits (62), Expect = 2.7
Identities = 18/99 (18%), Positives = 43/99 (43%), Gaps = 2/99 (2%)
Query: 9 NGAISEEVYVKQPPGFEDLKHPDYVYKDEIEMSMMGELKFFLRIQINQCKDEVYVHQSKY 68
N +++ +K G + + + + E + + KF +++ D++ K
Sbjct: 492 NTGKEQKITIKASSGLNETEIKKMIL--DAEQNSESDQKFEELVKVRNQGDQISHSTKKQ 549
Query: 69 TKDLLMKFKVDDCNEMNTPMHPTCTLSEEDADAKVDQKL 107
KDL V+D E+N ++ T + + K+++K+
Sbjct: 550 LKDLEKTINVNDQKEINEALNKLDTALKGENKTKIEEKI 588
>TERT_SCHPO (O13339) Telomerase reverse transcriptase (EC 2.7.7.-)
(Telomerase catalytic subunit)
Length = 988
Score = 28.1 bits (61), Expect = 3.6
Identities = 11/27 (40%), Positives = 16/27 (58%)
Query: 35 KDEIEMSMMGELKFFLRIQINQCKDEV 61
KD ++ M G K+F+RI I C D +
Sbjct: 572 KDLLKHRMFGRKKYFVRIDIKSCYDRI 598
>SKIL_MOUSE (Q60665) Ski-related oncogene SNO isoform SNON1/DE3
Length = 629
Score = 28.1 bits (61), Expect = 3.6
Identities = 18/51 (35%), Positives = 25/51 (48%)
Query: 46 LKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTLSE 96
L+ F QIN DE+Y++ S+ T D L KV N P TL++
Sbjct: 172 LREFSLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTD 222
>RPOB_XYLFT (Q87A32) DNA-directed RNA polymerase beta chain (EC
2.7.7.6) (RNAP beta subunit) (Transcriptase beta chain)
(RNA polymerase beta subunit)
Length = 1388
Score = 28.1 bits (61), Expect = 3.6
Identities = 17/64 (26%), Positives = 29/64 (44%), Gaps = 7/64 (10%)
Query: 15 EVYVKQPPG-------FEDLKHPDYVYKDEIEMSMMGELKFFLRIQINQCKDEVYVHQSK 67
E+Y PG ++L H + D ++SM+G +KF R+ + E ++ K
Sbjct: 375 EIYRMMRPGEPPTKEAAQNLFHNLFFTFDRYDLSMVGRMKFNRRVGRKEVAGEPVLYDKK 434
Query: 68 YTKD 71
Y D
Sbjct: 435 YFSD 438
>RPOB_XYLFA (Q9PA86) DNA-directed RNA polymerase beta chain (EC
2.7.7.6) (RNAP beta subunit) (Transcriptase beta chain)
(RNA polymerase beta subunit)
Length = 1388
Score = 28.1 bits (61), Expect = 3.6
Identities = 17/64 (26%), Positives = 29/64 (44%), Gaps = 7/64 (10%)
Query: 15 EVYVKQPPG-------FEDLKHPDYVYKDEIEMSMMGELKFFLRIQINQCKDEVYVHQSK 67
E+Y PG ++L H + D ++SM+G +KF R+ + E ++ K
Sbjct: 375 EIYRMMRPGEPPTKEAAQNLFHNLFFTFDRYDLSMVGRMKFNRRVGRKEVAGEPVLYDKK 434
Query: 68 YTKD 71
Y D
Sbjct: 435 YFSD 438
>PMC4_SCHPO (Q9Y821) RNA polymerase II mediator complex protein pmc4
Length = 239
Score = 28.1 bits (61), Expect = 3.6
Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
Query: 78 VDDCNEMNTPMHPTCTLSEE--DADAKVDQKLFRGIILFD 115
V++ +MN P PT T EE + ++ ++ +F G LFD
Sbjct: 193 VEEKTKMNYPASPTFTTQEENKEVESPANKDVFAGFDLFD 232
>ETR1_PELHO (Q9XH58) Ethylene receptor 1 (EC 2.7.3.-) (PhETR1)
Length = 740
Score = 27.7 bits (60), Expect = 4.6
Identities = 15/48 (31%), Positives = 25/48 (51%), Gaps = 2/48 (4%)
Query: 82 NEMNTPMHPTCTLSE--EDADAKVDQKLFRGIILFDDSILFLILRPIL 127
+EM TPMH LS ++ D +Q+L IL ++L ++ +L
Sbjct: 353 HEMRTPMHAIIALSSLLQETDLTSEQRLMVETILKSSNLLATLINDVL 400
>ETR1_LYCES (Q41342) Ethylene receptor 1 (EC 2.7.3.-) (LeETR1)
Length = 754
Score = 27.7 bits (60), Expect = 4.6
Identities = 15/48 (31%), Positives = 25/48 (51%), Gaps = 2/48 (4%)
Query: 82 NEMNTPMHPTCTLSE--EDADAKVDQKLFRGIILFDDSILFLILRPIL 127
+EM TPMH LS ++ D +Q+L IL ++L ++ +L
Sbjct: 367 HEMRTPMHAIIALSSLLQETDLTPEQRLMVETILKSSNLLATLINDVL 414
>DHAM_MOUSE (P47738) Aldehyde dehydrogenase, mitochondrial precursor
(EC 1.2.1.3) (ALDH class 2) (AHD-M1) (ALDHI) (ALDH-E2)
Length = 519
Score = 27.7 bits (60), Expect = 4.6
Identities = 21/65 (32%), Positives = 33/65 (50%), Gaps = 2/65 (3%)
Query: 10 GAISEEVYVKQPPGFEDLKHPDYVYKDEIEMSMMGELKF-FLRIQINQCKDEVY-VHQSK 67
GA ++ Y QP F D+K + K+EI +M LKF + + + D Y + +
Sbjct: 391 GAAADRGYFIQPTVFGDVKDGMTIAKEEIFGPVMQILKFKTIEEVVGRANDSKYGLAAAV 450
Query: 68 YTKDL 72
+TKDL
Sbjct: 451 FTKDL 455
>NFIB_CHICK (P17924) Nuclear factor 1 B-type (Nuclear factor 1/B)
(NF1-B) (NFI-B) (NF-I/B) (CCAAT-box binding
transcription factor) (CTF) (TGGCA-binding protein)
Length = 560
Score = 27.3 bits (59), Expect = 6.1
Identities = 12/38 (31%), Positives = 18/38 (46%)
Query: 66 SKYTKDLLMKFKVDDCNEMNTPMHPTCTLSEEDADAKV 103
+K KD+ +F+ D + HP C LS D K+
Sbjct: 78 AKLRKDIRQEFREDFVLTVTGKKHPCCVLSNPDQKGKI 115
>VGLB_VZVD (P09257) Glycoprotein B precursor (Glycoprotein II)
Length = 868
Score = 26.9 bits (58), Expect = 7.9
Identities = 15/62 (24%), Positives = 30/62 (48%)
Query: 23 GFEDLKHPDYVYKDEIEMSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCN 82
G + + DY Y EI + +G + ++ + + KD ++ YT+D L + D +
Sbjct: 593 GHHYVYYEDYRYVREIAVHDVGMISTYVDLNLTLLKDREFMPLQVYTRDELRDTGLLDYS 652
Query: 83 EM 84
E+
Sbjct: 653 EI 654
>RPOA_VIBVY (Q7MPG4) DNA-directed RNA polymerase alpha chain (EC
2.7.7.6) (RNAP alpha subunit) (Transcriptase alpha
chain) (RNA polymerase alpha subunit)
Length = 330
Score = 26.9 bits (58), Expect = 7.9
Identities = 16/63 (25%), Positives = 34/63 (53%), Gaps = 2/63 (3%)
Query: 50 LRIQINQCKDEVYVHQSKYTKDLLMKFKV--DDCNEMNTPMHPTCTLSEEDADAKVDQKL 107
L +++ + KDEV++ +K ++ + D E+ P H C L++++A+ + K+
Sbjct: 88 LAVRVAEGKDEVFITLNKSGSGPVVAGDITHDGDVEIANPEHVICHLTDDNAEIAMRIKV 147
Query: 108 FRG 110
RG
Sbjct: 148 ERG 150
>RPOA_VIBVU (Q8DE63) DNA-directed RNA polymerase alpha chain (EC
2.7.7.6) (RNAP alpha subunit) (Transcriptase alpha
chain) (RNA polymerase alpha subunit)
Length = 330
Score = 26.9 bits (58), Expect = 7.9
Identities = 16/63 (25%), Positives = 34/63 (53%), Gaps = 2/63 (3%)
Query: 50 LRIQINQCKDEVYVHQSKYTKDLLMKFKV--DDCNEMNTPMHPTCTLSEEDADAKVDQKL 107
L +++ + KDEV++ +K ++ + D E+ P H C L++++A+ + K+
Sbjct: 88 LAVRVAEGKDEVFITLNKSGSGPVVAGDITHDGDVEIANPEHVICHLTDDNAEIAMRIKV 147
Query: 108 FRG 110
RG
Sbjct: 148 ERG 150
>RPOA_VIBPA (Q87SZ0) DNA-directed RNA polymerase alpha chain (EC
2.7.7.6) (RNAP alpha subunit) (Transcriptase alpha
chain) (RNA polymerase alpha subunit)
Length = 330
Score = 26.9 bits (58), Expect = 7.9
Identities = 16/63 (25%), Positives = 34/63 (53%), Gaps = 2/63 (3%)
Query: 50 LRIQINQCKDEVYVHQSKYTKDLLMKFKV--DDCNEMNTPMHPTCTLSEEDADAKVDQKL 107
L +++ + KDEV++ +K ++ + D E+ P H C L++++A+ + K+
Sbjct: 88 LAVRVAEGKDEVFITLNKSGSGPVVAGDITHDGDVEIANPEHVICHLTDDNAEIAMRIKV 147
Query: 108 FRG 110
RG
Sbjct: 148 ERG 150
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.141 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,248,986
Number of Sequences: 164201
Number of extensions: 590096
Number of successful extensions: 1345
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1331
Number of HSP's gapped (non-prelim): 24
length of query: 129
length of database: 59,974,054
effective HSP length: 105
effective length of query: 24
effective length of database: 42,732,949
effective search space: 1025590776
effective search space used: 1025590776
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147714.14