BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147712.6 + phase: 0
(80 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YVCE_BACSU (P40767) Hypothetical protein yvcE (PSPA2) 35 0.035
MYHA_RAT (Q9JLT0) Myosin heavy chain, nonmuscle type B (Cellular... 34 0.077
SMC2_CHICK (Q90988) Structural maintenance of chromosome 2 (Chro... 33 0.10
PSO2_YEAST (P30620) DNA cross-link repair protein PSO2/SNM1 33 0.10
MYH9_CHICK (P14105) Myosin heavy chain, nonmuscle (Cellular myos... 33 0.10
CRS3_MOUSE (Q03402) Cysteine-rich secretory protein-3 precursor ... 33 0.10
MYHA_BOVIN (Q27991) Myosin heavy chain, nonmuscle type B (Cellul... 33 0.13
MYH9_RAT (Q62812) Myosin heavy chain, nonmuscle type A (Cellular... 33 0.13
MYH9_HUMAN (P35579) Myosin heavy chain, nonmuscle type A (Cellul... 33 0.13
AKA9_HUMAN (Q99996) A-kinase anchor protein 9 (Protein kinase A ... 33 0.17
RA50_AQUAE (O67124) Probable DNA double-strand break repair rad5... 32 0.22
MYHB_CHICK (P10587) Myosin heavy chain, gizzard smooth muscle 32 0.22
MYHA_HUMAN (P35580) Myosin heavy chain, nonmuscle type B (Cellul... 32 0.22
TPM3_MOUSE (P21107) Tropomyosin alpha 3 chain (Tropomyosin 3) (T... 32 0.29
TPM3_HUMAN (P06753) Tropomyosin alpha 3 chain (Tropomyosin 3) (T... 32 0.29
GOA5_HUMAN (Q8TBA6) Golgi autoantigen, golgin subfamily A member... 32 0.29
GOA5_MOUSE (Q9QYE6) Golgi autoantigen, golgin subfamily A member... 31 0.50
PSPA_BACSU (P54617) Phage shock protein A homolog 31 0.65
GAR2_SCHPO (P41891) Protein gar2 31 0.65
CRS1_MOUSE (Q03401) Cysteine-rich secretory protein-1 precursor ... 31 0.65
>YVCE_BACSU (P40767) Hypothetical protein yvcE (PSPA2)
Length = 473
Score = 35.0 bits (79), Expect = 0.035
Identities = 18/62 (29%), Positives = 36/62 (58%)
Query: 2 SVASASEKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIRVCKNMIK 61
S A EK+L + +Q K+E+EL ++N++ + +++ + + + +EEI+ K IK
Sbjct: 49 SSIEAKEKELTELQENQSKIEKELKDINDKALDTSNKIEDKKEENDKTKEEIKKLKKEIK 108
Query: 62 CT 63
T
Sbjct: 109 ET 110
>MYHA_RAT (Q9JLT0) Myosin heavy chain, nonmuscle type B (Cellular
myosin heavy chain, type B) (Nonmuscle myosin heavy
chain-B) (NMMHC-B)
Length = 1976
Score = 33.9 bits (76), Expect = 0.077
Identities = 16/47 (34%), Positives = 29/47 (61%)
Query: 8 EKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIR 54
EK +++KL+ E ++ +QIAEL ++ E VQ ++EEE++
Sbjct: 1047 EKTRQELEKAKRKLDGETTDLQDQIAELQAQVDELKVQLTKKEEELQ 1093
>SMC2_CHICK (Q90988) Structural maintenance of chromosome 2
(Chromosome scaffold protein ScII)
Length = 1189
Score = 33.5 bits (75), Expect = 0.10
Identities = 19/63 (30%), Positives = 34/63 (53%), Gaps = 1/63 (1%)
Query: 14 KRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIRVCKNMIKCTVFSDRPKEVK 73
++S+ K +E+L+ + IAE +T Q + EEE + +N +K ++R KE+K
Sbjct: 734 QQSAYHKQQEDLLALKKTIAECEETLKKTEESQRKAEEEYKALENKMK-NAEAERGKEIK 792
Query: 74 TGQ 76
Q
Sbjct: 793 NAQ 795
>PSO2_YEAST (P30620) DNA cross-link repair protein PSO2/SNM1
Length = 661
Score = 33.5 bits (75), Expect = 0.10
Identities = 13/44 (29%), Positives = 31/44 (69%), Gaps = 1/44 (2%)
Query: 22 EEELMNVN-NQIAELNSETGETVVQQLEEEEEIRVCKNMIKCTV 64
EEEL++++ N+++ + + T + +V +LEE+ ++V +I+C +
Sbjct: 106 EEELVSLHDNEVSAIENRTEDRIVTELEEQVNVKVSTEVIQCPI 149
>MYH9_CHICK (P14105) Myosin heavy chain, nonmuscle (Cellular myosin
heavy chain) (NMMHC)
Length = 1959
Score = 33.5 bits (75), Expect = 0.10
Identities = 15/47 (31%), Positives = 30/47 (62%)
Query: 8 EKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIR 54
EK +++KLE + ++++QIAEL ++ E +Q ++EEE++
Sbjct: 1040 EKQRQELEKTRRKLEGDSSDLHDQIAELQAQIAELKIQLSKKEEELQ 1086
>CRS3_MOUSE (Q03402) Cysteine-rich secretory protein-3 precursor
(Crisp-3) (Acidic epididymal glycoprotein 2)
(Sperm-coating glycoprotein 2) (SCP 2)
Length = 241
Score = 33.5 bits (75), Expect = 0.10
Identities = 19/74 (25%), Positives = 41/74 (54%), Gaps = 2/74 (2%)
Query: 5 SASEKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVV-QQLEEEEEIRVCKNMIKCT 63
++ E L+ +S+K ++EE+++ +NQ+ S +G ++ + + ++ + KCT
Sbjct: 22 NSQENSLEKLSTSKKSVQEEIVSKHNQLRRKVSPSGSDLLNMEWNYDAQVNAQQRADKCT 81
Query: 64 VFSDRPKEVKTGQL 77
FS P E++T L
Sbjct: 82 -FSHSPIELRTTNL 94
>MYHA_BOVIN (Q27991) Myosin heavy chain, nonmuscle type B (Cellular
myosin heavy chain, type B) (Nonmuscle myosin heavy
chain-B) (NMMHC-B)
Length = 1976
Score = 33.1 bits (74), Expect = 0.13
Identities = 15/47 (31%), Positives = 29/47 (60%)
Query: 8 EKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIR 54
EK +++KL+ E ++ +QIAEL ++ E +Q ++EEE++
Sbjct: 1047 EKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKIQVAKKEEELQ 1093
>MYH9_RAT (Q62812) Myosin heavy chain, nonmuscle type A (Cellular
myosin heavy chain, type A) (Nonmuscle myosin heavy
chain-A) (NMMHC-A)
Length = 1961
Score = 33.1 bits (74), Expect = 0.13
Identities = 15/47 (31%), Positives = 30/47 (62%)
Query: 8 EKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIR 54
EK +++KLE + ++++QIAEL ++ E +Q ++EEE++
Sbjct: 1040 EKQRQELEKTRRKLEGDSTDLSDQIAELQAQIAELKMQLAKKEEELQ 1086
>MYH9_HUMAN (P35579) Myosin heavy chain, nonmuscle type A (Cellular
myosin heavy chain, type A) (Nonmuscle myosin heavy
chain-A) (NMMHC-A)
Length = 1960
Score = 33.1 bits (74), Expect = 0.13
Identities = 15/47 (31%), Positives = 30/47 (62%)
Query: 8 EKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIR 54
EK +++KLE + ++++QIAEL ++ E +Q ++EEE++
Sbjct: 1040 EKQRQELEKTRRKLEGDSTDLSDQIAELQAQIAELKMQLAKKEEELQ 1086
>AKA9_HUMAN (Q99996) A-kinase anchor protein 9 (Protein kinase A
anchoring protein 9) (PRKA9) (A-kinase anchor protein 450
kDa) (AKAP 450) (A-kinase anchor protein 350 kDa) (AKAP
350) (hgAKAP 350) (AKAP 120 like protein) (Hyperion
protein) (Yotiao protein
Length = 3911
Score = 32.7 bits (73), Expect = 0.17
Identities = 16/56 (28%), Positives = 32/56 (56%), Gaps = 2/56 (3%)
Query: 6 ASEKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEE--EEIRVCKNM 59
A + ++ +R Q++L+EE+ +N Q+A+ +S E +V + E EE+ K +
Sbjct: 1623 AHMRQMERQREDQEQLQEEIKRLNRQLAQRSSIDNENLVSERERVLLEELEALKQL 1678
Score = 30.0 bits (66), Expect = 1.1
Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 5/46 (10%)
Query: 8 EKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEI 53
E+ + K +LE+EL+ +N++ EL +E QQ++EE E+
Sbjct: 1947 ERQIQEKTDIIDRLEQELLCASNRLQELEAEQ-----QQIQEEREL 1987
Score = 27.7 bits (60), Expect = 5.5
Identities = 18/66 (27%), Positives = 33/66 (49%), Gaps = 3/66 (4%)
Query: 11 LDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIRVCKNMIKC---TVFSD 67
L + Q++++EE ++ Q + +E G Q L+E E++ K ++C V D
Sbjct: 1971 LQELEAEQQQIQEERELLSRQKEAMKAEAGPVEQQLLQETEKLMKEKLEVQCQAEKVRDD 2030
Query: 68 RPKEVK 73
K+VK
Sbjct: 2031 LQKQVK 2036
>RA50_AQUAE (O67124) Probable DNA double-strand break repair rad50
ATPase
Length = 978
Score = 32.3 bits (72), Expect = 0.22
Identities = 17/58 (29%), Positives = 37/58 (63%), Gaps = 4/58 (6%)
Query: 8 EKDLDS--KRSSQK--KLEEELMNVNNQIAELNSETGETVVQQLEEEEEIRVCKNMIK 61
E+++D+ K +QK L+EE+ + N++ EL E E + +++++ EE+R+ K ++
Sbjct: 520 EREIDTTLKLYAQKINSLKEEMEKLRNEVEELRKEIPENLKERIKKLEELRIEKEKLE 577
>MYHB_CHICK (P10587) Myosin heavy chain, gizzard smooth muscle
Length = 1978
Score = 32.3 bits (72), Expect = 0.22
Identities = 16/47 (34%), Positives = 27/47 (57%)
Query: 8 EKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIR 54
EK ++KLE E +++ QIAEL ++ E Q ++EEE++
Sbjct: 1052 EKSRQELEKIKRKLEGESSDLHEQIAELQAQIAELKAQLAKKEEELQ 1098
>MYHA_HUMAN (P35580) Myosin heavy chain, nonmuscle type B (Cellular
myosin heavy chain, type B) (Nonmuscle myosin heavy
chain-B) (NMMHC-B)
Length = 1976
Score = 32.3 bits (72), Expect = 0.22
Identities = 15/47 (31%), Positives = 29/47 (60%)
Query: 8 EKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIR 54
EK +++KL+ E ++ +QIAEL ++ E +Q ++EEE++
Sbjct: 1047 EKTRQELEKAKRKLDGETTDLQDQIAELQAQIDELKLQLAKKEEELQ 1093
>TPM3_MOUSE (P21107) Tropomyosin alpha 3 chain (Tropomyosin 3)
(Tropomyosin gamma)
Length = 284
Score = 32.0 bits (71), Expect = 0.29
Identities = 16/55 (29%), Positives = 31/55 (56%)
Query: 7 SEKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIRVCKNMIK 61
+E+ + S +LEEEL NV N + L ++ + ++ + EEEI++ + +K
Sbjct: 179 TEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLK 233
>TPM3_HUMAN (P06753) Tropomyosin alpha 3 chain (Tropomyosin 3)
(Tropomyosin gamma) (hTM5)
Length = 284
Score = 32.0 bits (71), Expect = 0.29
Identities = 16/55 (29%), Positives = 31/55 (56%)
Query: 7 SEKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIRVCKNMIK 61
+E+ + S +LEEEL NV N + L ++ + ++ + EEEI++ + +K
Sbjct: 179 TEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLK 233
>GOA5_HUMAN (Q8TBA6) Golgi autoantigen, golgin subfamily A member 5
(Golgin-84) (RET-fused gene 5 protein) (Ret-II protein)
Length = 731
Score = 32.0 bits (71), Expect = 0.29
Identities = 16/51 (31%), Positives = 27/51 (52%)
Query: 4 ASASEKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIR 54
++AS +L+ R ++ EE+ + QI +L SE + QQ+ E E R
Sbjct: 461 STASSMELEELRHEKEMQREEIQKLMGQIHQLRSELQDMEAQQVNEAESAR 511
>GOA5_MOUSE (Q9QYE6) Golgi autoantigen, golgin subfamily A member 5
(Sumiko protein) (Ret-II protein)
Length = 729
Score = 31.2 bits (69), Expect = 0.50
Identities = 15/51 (29%), Positives = 28/51 (54%)
Query: 4 ASASEKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIR 54
++AS +L+ R ++ +EE+ + Q+ +L SE + QQ+ E E R
Sbjct: 459 STASSMELEELRHEKEMQKEEIQKLMGQMHQLRSELQDMEAQQVSEAESAR 509
>PSPA_BACSU (P54617) Phage shock protein A homolog
Length = 226
Score = 30.8 bits (68), Expect = 0.65
Identities = 11/47 (23%), Positives = 27/47 (57%)
Query: 11 LDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIRVCK 57
LD + +K +++ L N+N+ +A++ +ET + ++ + E C+
Sbjct: 18 LDKAENPEKMVDQYLRNMNSDLAKVKAETAAVMAEEQRAKREYHECQ 64
>GAR2_SCHPO (P41891) Protein gar2
Length = 500
Score = 30.8 bits (68), Expect = 0.65
Identities = 16/54 (29%), Positives = 30/54 (54%)
Query: 2 SVASASEKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQQLEEEEEIRV 55
S +S+SE + S SS + EEE++ + E +SE+ + + EEE +++
Sbjct: 103 SESSSSESESSSSESSSSESEEEVIVKTEEKKESSSESSSSSESEEEEEAVVKI 156
>CRS1_MOUSE (Q03401) Cysteine-rich secretory protein-1 precursor
(CRISP-1) (Acidic epididymal glycoprotein 1)
(Sperm-coating glycoprotein 1) (SCP 1)
Length = 244
Score = 30.8 bits (68), Expect = 0.65
Identities = 18/74 (24%), Positives = 42/74 (56%), Gaps = 2/74 (2%)
Query: 5 SASEKDLDSKRSSQKKLEEELMNVNNQIAELNSETGETVVQ-QLEEEEEIRVCKNMIKCT 63
S+ E L+ +++ ++EE+++ +NQ+ + S +G +++ + + ++ + KCT
Sbjct: 22 SSQENRLEKLSTTKMSVQEEIVSKHNQLRRMVSPSGSDLLKMEWNYDAQVNAQQWADKCT 81
Query: 64 VFSDRPKEVKTGQL 77
FS P E++T L
Sbjct: 82 -FSHSPIELRTTNL 94
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.304 0.122 0.303
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,053,970
Number of Sequences: 164201
Number of extensions: 273362
Number of successful extensions: 1905
Number of sequences better than 10.0: 137
Number of HSP's better than 10.0 without gapping: 82
Number of HSP's successfully gapped in prelim test: 55
Number of HSP's that attempted gapping in prelim test: 1736
Number of HSP's gapped (non-prelim): 221
length of query: 80
length of database: 59,974,054
effective HSP length: 56
effective length of query: 24
effective length of database: 50,778,798
effective search space: 1218691152
effective search space used: 1218691152
T: 11
A: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147712.6