BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147538.7 - phase: 0 /pseudo
(113 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PML1_MOUSE (O54990) Prominin 1 precursor (Prominin-like protein ... 32 0.20
YNH7_YEAST (P53940) Hypothetical 58.9 kDa protein in TPM1-MKS1 i... 32 0.34
YK69_YEAST (P36165) Hypothetical 102.7 kDa protein in PRP16-SRP4... 31 0.45
INA8_HUMAN (P32881) Interferon alpha-8 precursor (Interferon alp... 31 0.58
PESC_MOUSE (Q9EQ61) Pescadillo homolog 1 30 0.76
CBF1_KLULA (P49379) Centromere-binding protein 1 (CBP-1) (Centro... 30 0.99
KLP5_SCHPO (O14343) Kinesin-like protein 5 30 1.3
FKB4_SPOFR (Q26486) 46 kDa FK506-binding nuclear protein (EC 5.2... 29 1.7
YG58_YEAST (P53313) Hypothetical 86.6 kDa protein in PFK1-TDS4 i... 29 2.2
Y857_METJA (Q58267) Hypothetical protein MJ0857 29 2.2
KAP2_RAT (P12368) cAMP-dependent protein kinase type II-alpha re... 29 2.2
UB11_MOUSE (Q99K46) Ubiquitin carboxyl-terminal hydrolase 11 (EC... 28 2.9
SA3_HUMAN (Q9UJ98) Cohesin subunit SA-3 (Stromal antigen 3) (Str... 28 2.9
KEX1_YEAST (P09620) Carboxypeptidase KEX1 precursor (EC 3.4.16.6... 28 2.9
YP46_YEAST (Q12080) Hypothetical protein YPL146C 28 3.8
YEM8_YEAST (P40026) Hypothetical 53.8 kDa protein in PIP1-GLN3 i... 28 3.8
TML1_ARATH (P33543) Putative kinase-like protein TMKL1 precursor 28 4.9
SM1A_RAT (Q9Z1M9) Structural maintenance of chromosome 1-like 1 ... 28 4.9
SM1A_MOUSE (Q9CU62) Structural maintenance of chromosome 1-like ... 28 4.9
SM1A_HUMAN (Q14683) Structural maintenance of chromosome 1-like ... 28 4.9
>PML1_MOUSE (O54990) Prominin 1 precursor (Prominin-like protein 1)
(Antigen AC133 homolog)
Length = 867
Score = 32.3 bits (72), Expect = 0.20
Identities = 23/78 (29%), Positives = 40/78 (50%), Gaps = 1/78 (1%)
Query: 12 VRKSLNILQFSLNRPSSLCCVDEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLA 71
+R SL+ L SLN S L VD + + E+D+ D+E V+ + + + I +Q +
Sbjct: 320 IRPSLSSLGSSLNS-SQLPSVDRELNTVTEVDKTDLESLVKRGYTTIDEIPNTIQNQTVD 378
Query: 72 QLTEKKNNPCGFQASIKD 89
+ + KN ++IKD
Sbjct: 379 VIKDVKNTLDSISSNIKD 396
>YNH7_YEAST (P53940) Hypothetical 58.9 kDa protein in TPM1-MKS1
intergenic region
Length = 528
Score = 31.6 bits (70), Expect = 0.34
Identities = 22/73 (30%), Positives = 37/73 (50%), Gaps = 5/73 (6%)
Query: 27 SSLCCVDEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQL-----TEKKNNPC 81
S++ D D ++E + VE+HV +F + N N I D DL L + K+NN
Sbjct: 437 STMETADSDCYCDLEKSYDSVEEHVLSSFEAPNLNNEVIEDDDLGDLINERDSRKRNNRR 496
Query: 82 GFQASIKDSFEVK 94
+++I ++ E K
Sbjct: 497 FDESNINNNNETK 509
>YK69_YEAST (P36165) Hypothetical 102.7 kDa protein in PRP16-SRP40
intergenic region
Length = 910
Score = 31.2 bits (69), Expect = 0.45
Identities = 20/80 (25%), Positives = 39/80 (48%), Gaps = 7/80 (8%)
Query: 32 VDEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSF 91
V+ED + ++ + + V N +GN +K+ D D +TE +NN + ++
Sbjct: 60 VEEDAGKRLQTGKNKTTNKVNFNLDTGNEDKL---DDDQETVTENENN----DIEMVETD 112
Query: 92 EVKLEQELNGLRQKAGGFLF 111
E + E++ + L K FL+
Sbjct: 113 EGEDERQGSSLASKCKSFLY 132
>INA8_HUMAN (P32881) Interferon alpha-8 precursor (Interferon
alpha-B2) (Interferon alpha-B) (LeIF B)
Length = 189
Score = 30.8 bits (68), Expect = 0.58
Identities = 21/85 (24%), Positives = 37/85 (42%), Gaps = 4/85 (4%)
Query: 18 ILQFSLNRPSSLCCVDEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKK 77
IL + R S C+ + D E +E D + + +S H + Q L K
Sbjct: 39 ILLAQMRRISPFSCLKDRHDFEFPQEEFDDKQFQKAQAISVLHEMI----QQTFNLFSTK 94
Query: 78 NNPCGFQASIKDSFEVKLEQELNGL 102
++ ++ D F ++L+Q+LN L
Sbjct: 95 DSSAALDETLLDEFYIELDQQLNDL 119
>PESC_MOUSE (Q9EQ61) Pescadillo homolog 1
Length = 584
Score = 30.4 bits (67), Expect = 0.76
Identities = 17/71 (23%), Positives = 33/71 (45%)
Query: 33 DEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSFE 92
DED D E ++ E+ E+ V+V +++++ + +L EKK ++D
Sbjct: 460 DEDDDNEGDVAAENEEEDVEVESEEEEEEEVHLSALEQHRLEEKKPQVMAGTVKLEDKQR 519
Query: 93 VKLEQELNGLR 103
+ E+E R
Sbjct: 520 LAQEEESEAKR 530
>CBF1_KLULA (P49379) Centromere-binding protein 1 (CBP-1)
(Centromere-binding factor 1) (Centromere promoter
factor 1)
Length = 359
Score = 30.0 bits (66), Expect = 0.99
Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 6/52 (11%)
Query: 33 DEDGDEEMEIDEEDVEDHVQVNFVSGNHNKM-----NIADQDLAQLTEKKNN 79
DED DE+ E +EED +DHV ++ V + + + + D+D AQ K N
Sbjct: 121 DEDEDEDEE-EEEDEDDHVDIDDVDKDPDAVIDEDDDEEDEDQAQRRRGKKN 171
>KLP5_SCHPO (O14343) Kinesin-like protein 5
Length = 883
Score = 29.6 bits (65), Expect = 1.3
Identities = 11/37 (29%), Positives = 23/37 (61%)
Query: 56 VSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSFE 92
+S + +++ + Q LA+L+ K NN GF ++ +F+
Sbjct: 72 LSATNRRLSTSQQSLARLSRKSNNSAGFGRDLRYAFD 108
>FKB4_SPOFR (Q26486) 46 kDa FK506-binding nuclear protein (EC
5.2.1.8) (Peptidyl-prolyl cis-trans isomerase) (PPIase)
(Rotamase)
Length = 412
Score = 29.3 bits (64), Expect = 1.7
Identities = 20/67 (29%), Positives = 32/67 (46%), Gaps = 1/67 (1%)
Query: 33 DEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKN-NPCGFQASIKDSF 91
+ED DE+ E +E+D ED + K A+QD T K++ + S K
Sbjct: 194 EEDDDEDEEDEEDDDEDDEEEEEAPKKKKKQPAAEQDSTLDTSKESVDMSKLSKSQKRRL 253
Query: 92 EVKLEQE 98
+ KL+Q+
Sbjct: 254 KKKLQQQ 260
>YG58_YEAST (P53313) Hypothetical 86.6 kDa protein in PFK1-TDS4
intergenic region
Length = 767
Score = 28.9 bits (63), Expect = 2.2
Identities = 22/73 (30%), Positives = 35/73 (47%), Gaps = 9/73 (12%)
Query: 33 DEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASI----- 87
+ED D E+D + ED V +V+ + +K D D+ ++K+N G ++
Sbjct: 553 NEDADANWEVDVDSEEDDVDGEWVTMDSDKE--YDVDMEDSDDEKDNAKGKESDSDLELS 610
Query: 88 --KDSFEVKLEQE 98
D EVK EQE
Sbjct: 611 DDDDEKEVKDEQE 623
>Y857_METJA (Q58267) Hypothetical protein MJ0857
Length = 274
Score = 28.9 bits (63), Expect = 2.2
Identities = 19/61 (31%), Positives = 32/61 (52%), Gaps = 6/61 (9%)
Query: 54 NFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSFEVK-LEQELNGLRQKAGGFLFL 112
N GN + + DL +LTEK +N +I++ F K + ++ L++K GF+ L
Sbjct: 214 NITYGNFENIELIKTDLGKLTEKYSN---IMTNIQEKFSFKGIHKQDKPLKRK--GFILL 268
Query: 113 K 113
K
Sbjct: 269 K 269
>KAP2_RAT (P12368) cAMP-dependent protein kinase type II-alpha
regulatory subunit (Fragment)
Length = 370
Score = 28.9 bits (63), Expect = 2.2
Identities = 22/90 (24%), Positives = 38/90 (41%), Gaps = 16/90 (17%)
Query: 21 FSLNRPSSLCCVDEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNP 80
F S + ++EDG E + D+ED+E + F ++++ + E+ N+P
Sbjct: 27 FHAQESSGVPVIEEDGQSESDSDDEDLEVPIPAKFT----RRVSVCAETFNPDEEEDNDP 82
Query: 81 -----------CGFQASIKDSFEVK-LEQE 98
C Q + KD K L+QE
Sbjct: 83 RVVHPKTDEQRCRLQEACKDILLFKNLDQE 112
>UB11_MOUSE (Q99K46) Ubiquitin carboxyl-terminal hydrolase 11 (EC
3.1.2.15) (Ubiquitin thiolesterase 11)
(Ubiquitin-specific processing protease 11)
(Deubiquitinating enzyme 11) (Fragment)
Length = 797
Score = 28.5 bits (62), Expect = 2.9
Identities = 18/35 (51%), Positives = 24/35 (68%), Gaps = 2/35 (5%)
Query: 17 NILQFSLNRPSSLCCVDE-DGDEEMEIDE-EDVED 49
NIL + L+R + DE DGDE+++ DE EDVED
Sbjct: 457 NILMYRLSRYVTKPTSDEDDGDEKVDEDEDEDVED 491
>SA3_HUMAN (Q9UJ98) Cohesin subunit SA-3 (Stromal antigen 3)
(Stromalin 3) (SCC3 homolog 3)
Length = 1225
Score = 28.5 bits (62), Expect = 2.9
Identities = 14/52 (26%), Positives = 27/52 (51%)
Query: 24 NRPSSLCCVDEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTE 75
N+ L ++ED +EE+EI +E E+ + + +++ + DL TE
Sbjct: 1168 NQLMRLSLMEEDEEEELEIQDESNEERQDTDMQASSYSSTSERGLDLLDSTE 1219
>KEX1_YEAST (P09620) Carboxypeptidase KEX1 precursor (EC 3.4.16.6)
(Carboxypeptidase D)
Length = 729
Score = 28.5 bits (62), Expect = 2.9
Identities = 19/66 (28%), Positives = 34/66 (50%), Gaps = 7/66 (10%)
Query: 33 DEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSFE 92
D+DGD++ + D++D +DH + + + D D EK++N G + S S E
Sbjct: 545 DKDGDDDNDNDDDDEDDH------NSEGDDDDDDDDDEDDNNEKQSNQ-GLEDSRHKSSE 597
Query: 93 VKLEQE 98
+ E+E
Sbjct: 598 YEQEEE 603
>YP46_YEAST (Q12080) Hypothetical protein YPL146C
Length = 455
Score = 28.1 bits (61), Expect = 3.8
Identities = 19/73 (26%), Positives = 36/73 (49%), Gaps = 2/73 (2%)
Query: 33 DEDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSFE 92
+ +EE E +EE+ E+ + SG+ ++ ++ + KK + + +
Sbjct: 276 ESSSNEEEEEEEEENENENESTQCSGSDKEIKLSINK--PVKNKKKTKYQRNKAKRHEEK 333
Query: 93 VKLEQELNGLRQK 105
VKL+QEL LRQ+
Sbjct: 334 VKLQQELKELRQR 346
>YEM8_YEAST (P40026) Hypothetical 53.8 kDa protein in PIP1-GLN3
intergenic region
Length = 464
Score = 28.1 bits (61), Expect = 3.8
Identities = 15/61 (24%), Positives = 30/61 (48%)
Query: 44 EEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSFEVKLEQELNGLR 103
++D ++ V NF+S N +D++ + L N G +A D F+ L++ + R
Sbjct: 38 DDDDDEKVHPNFISDPENDSLNSDEEFSSLENSDLNLSGAKAESGDDFDPILKRTIISKR 97
Query: 104 Q 104
+
Sbjct: 98 K 98
>TML1_ARATH (P33543) Putative kinase-like protein TMKL1 precursor
Length = 674
Score = 27.7 bits (60), Expect = 4.9
Identities = 17/62 (27%), Positives = 30/62 (47%)
Query: 34 EDGDEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSFEV 93
E+GDEE EI E++ + V F G + ++ Q+ EK + ++A + D +
Sbjct: 339 EEGDEEDEIGEKEGGEGKLVVFQGGENLTLDDVLNATGQVMEKTSYGTVYKAKLSDGGNI 398
Query: 94 KL 95
L
Sbjct: 399 AL 400
>SM1A_RAT (Q9Z1M9) Structural maintenance of chromosome 1-like 1
protein (SMC-protein)
Length = 1233
Score = 27.7 bits (60), Expect = 4.9
Identities = 17/72 (23%), Positives = 32/72 (43%), Gaps = 10/72 (13%)
Query: 37 DEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSFEVKLE 96
++ ++ DE ++E M I D+ +AQL + KN ++ + D
Sbjct: 839 EQTVKKDENEIE-----KLKKEEQRHMKIIDETMAQLQDLKNQHLAKKSEVNDK-----N 888
Query: 97 QELNGLRQKAGG 108
E+ +R+K GG
Sbjct: 889 HEMEEIRKKLGG 900
>SM1A_MOUSE (Q9CU62) Structural maintenance of chromosome 1-like 1
protein (SMC1alpha protein) (Chromosome segregation
protein SmcB) (Sb1.8)
Length = 1233
Score = 27.7 bits (60), Expect = 4.9
Identities = 17/72 (23%), Positives = 32/72 (43%), Gaps = 10/72 (13%)
Query: 37 DEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSFEVKLE 96
++ ++ DE ++E M I D+ +AQL + KN ++ + D
Sbjct: 839 EQTVKKDENEIE-----KLKKEEQRHMKIIDETMAQLQDLKNQHLAKKSEVNDK-----N 888
Query: 97 QELNGLRQKAGG 108
E+ +R+K GG
Sbjct: 889 HEMEEIRKKLGG 900
>SM1A_HUMAN (Q14683) Structural maintenance of chromosome 1-like 1
protein (SMC1alpha protein) (SB1.8/DXS423E protein)
(Sb1.8)
Length = 1233
Score = 27.7 bits (60), Expect = 4.9
Identities = 17/72 (23%), Positives = 32/72 (43%), Gaps = 10/72 (13%)
Query: 37 DEEMEIDEEDVEDHVQVNFVSGNHNKMNIADQDLAQLTEKKNNPCGFQASIKDSFEVKLE 96
++ ++ DE ++E M I D+ +AQL + KN ++ + D
Sbjct: 839 EQTVKKDENEIE-----KLKKEEQRHMKIIDETMAQLQDLKNQHLAKKSEVNDK-----N 888
Query: 97 QELNGLRQKAGG 108
E+ +R+K GG
Sbjct: 889 HEMEEIRKKLGG 900
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.314 0.133 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,057,941
Number of Sequences: 164201
Number of extensions: 548989
Number of successful extensions: 2215
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 36
Number of HSP's that attempted gapping in prelim test: 2153
Number of HSP's gapped (non-prelim): 86
length of query: 113
length of database: 59,974,054
effective HSP length: 89
effective length of query: 24
effective length of database: 45,360,165
effective search space: 1088643960
effective search space used: 1088643960
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147538.7