BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147536.6 + phase: 0
(281 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LHS1_YEAST (P36016) Heat shock protein 70 homolog LHS1 precursor 34 0.35
LNT_PROMP (Q7V0G7) Apolipoprotein N-acyltransferase (EC 2.3.1.-)... 33 0.78
C166_CHICK (P42292) CD166 antigen precursor (Activated leukocyte... 33 0.78
BAF1_YEAST (P14164) Transcription factor BAF1 (ARS binding facto... 33 0.78
RRPL_HRSVA (P28887) RNA polymerase beta subunit (EC 2.7.7.48) (L... 31 3.0
SLK6_MOUSE (Q8C110) SLIT and NTRK-like protein 6 precursor 30 5.0
PTP3_DICDI (P54637) Protein-tyrosine phosphatase 3 (EC 3.1.3.48)... 30 5.0
P53_CRIGR (O09185) Cellular tumor antigen p53 (Tumor suppressor ... 30 5.0
ICAL_BOVIN (P20811) Calpain inhibitor (Calpastatin) 30 5.0
HMG4_HUMAN (O15347) High mobility group protein 4 (HMG-4) (High ... 30 5.0
C166_RAT (O35112) CD166 antigen precursor (Activated leukocyte-c... 30 5.0
C166_RABIT (O46651) CD166 antigen (Activated leukocyte-cell adhe... 30 5.0
C166_MOUSE (Q61490) CD166 antigen precursor (Activated leukocyte... 30 5.0
C166_HUMAN (Q13740) CD166 antigen precursor (Activated leukocyte... 30 5.0
C166_BOVIN (Q9BH13) CD166 antigen precursor (Activated leukocyte... 30 5.0
UN17_CAEEL (P34711) Vesicular acetylcholine transporter unc-17 (... 30 6.6
DYNA_RAT (P28023) Dynactin 1 (150 kDa dynein-associated polypept... 30 6.6
DYNA_MOUSE (O08788) Dynactin 1 (150 kDa dynein-associated polype... 30 6.6
VT3C_CAPVK (P18388) T3C protein 30 8.6
NRM1_CHICK (P51027) Natural resistance-associated macrophage pro... 30 8.6
>LHS1_YEAST (P36016) Heat shock protein 70 homolog LHS1 precursor
Length = 881
Score = 34.3 bits (77), Expect = 0.35
Identities = 18/58 (31%), Positives = 32/58 (55%), Gaps = 1/58 (1%)
Query: 196 LFESTTDAEFNRLKSSPPPKFKFLQEAEEKLRRKMQDDNKGINGNEVDNSLITIIVDK 253
L +S ++ N +K F+ + EEKL+RK++ + K N NE ++++I DK
Sbjct: 801 LIKSGDESRLNEIKKLHLRNFRLQKRKEEKLKRKLEQE-KSRNNNETESTVINSADDK 857
>LNT_PROMP (Q7V0G7) Apolipoprotein N-acyltransferase (EC 2.3.1.-)
(ALP N-acyltransferase)
Length = 496
Score = 33.1 bits (74), Expect = 0.78
Identities = 21/67 (31%), Positives = 33/67 (48%)
Query: 4 LSSVGLGLSIVFGCLLLALVAEVYYLLWWKKKIIKREIENDYGNGNPLKELFYMFCWKRP 63
+SS+ + +SI+FGC ++ + + L KK + K+EI N +K L F W
Sbjct: 81 ISSLIISISILFGCSIIGGILVYLWGLLVKKILQKKEISNMNSLRLTIKVLLLSFAWGIG 140
Query: 64 STSLRQT 70
L QT
Sbjct: 141 EFILSQT 147
>C166_CHICK (P42292) CD166 antigen precursor (Activated
leukocyte-cell adhesion molecule) (SC1 glycoprotein)
(BEN glycoprotein) (DM-GRASP protein) (JC7 protein)
Length = 588
Score = 33.1 bits (74), Expect = 0.78
Identities = 20/45 (44%), Positives = 27/45 (59%), Gaps = 1/45 (2%)
Query: 11 LSIVFGCLLLALVAEVYYLLWWKK-KIIKREIENDYGNGNPLKEL 54
+ IV G LL+ALVA V Y L+ KK K + ++ D GN K+L
Sbjct: 535 VGIVVGLLLVALVAGVVYWLYVKKSKTASKHVDKDLGNIEENKKL 579
>BAF1_YEAST (P14164) Transcription factor BAF1 (ARS binding factor
1) (Protein ABF1) (Bidirectionally acting factor)
(SFB-B) (DNA replication enhancer-binding protein OBF1)
Length = 731
Score = 33.1 bits (74), Expect = 0.78
Identities = 13/42 (30%), Positives = 22/42 (51%)
Query: 238 NGNEVDNSLITIIVDKKYEREVNHHQCHLQQYHSSTSQVLPL 279
N ++VD+ ++ + V+ +Y + NHH H Q H V L
Sbjct: 668 NDDDVDDVMVDVDVESQYNKNTNHHNNHHSQPHHDEEDVAGL 709
>RRPL_HRSVA (P28887) RNA polymerase beta subunit (EC 2.7.7.48)
(Large structural protein) (L protein)
Length = 2165
Score = 31.2 bits (69), Expect = 3.0
Identities = 17/43 (39%), Positives = 23/43 (52%)
Query: 225 KLRRKMQDDNKGINGNEVDNSLITIIVDKKYEREVNHHQCHLQ 267
KL K +D K NG + DNS+IT I+ V +Q HL+
Sbjct: 130 KLGLKEKDKIKSNNGQDEDNSVITTIIKDDILSAVKDNQSHLK 172
>SLK6_MOUSE (Q8C110) SLIT and NTRK-like protein 6 precursor
Length = 840
Score = 30.4 bits (67), Expect = 5.0
Identities = 21/79 (26%), Positives = 39/79 (48%), Gaps = 7/79 (8%)
Query: 4 LSSVGLGLSIVFGCLLLALVAEVYYLLWWKKKIIKREIENDYGNGNPLKELFYMFCWK-- 61
LS + LGL IVF ++ V ++L +++ K+++E + +P+ + M+ K
Sbjct: 606 LSVLILGLLIVFITIVFCAAGIVVFVLHRRRRYKKKKVEEQLRDNSPVHLQYSMYGHKTT 665
Query: 62 -----RPSTSLRQTGFTPP 75
RPS+SL + P
Sbjct: 666 HHTTERPSSSLYEQHMVSP 684
>PTP3_DICDI (P54637) Protein-tyrosine phosphatase 3 (EC 3.1.3.48)
(Protein-tyrosine-phosphate phosphohydrolase 3)
Length = 989
Score = 30.4 bits (67), Expect = 5.0
Identities = 21/67 (31%), Positives = 30/67 (44%), Gaps = 4/67 (5%)
Query: 169 PIASPHFFTPMNCSPYCSPYNQHGFNPLFESTTDAEFNRLKSSPPP----KFKFLQEAEE 224
P ASP F+ + S N + ST+ + N + SSPPP F L E E
Sbjct: 360 PNASPFSFSSSSLFSNSSLSNSGSGSASTTSTSTSSSNSMSSSPPPSLKTSFSQLDEDRE 419
Query: 225 KLRRKMQ 231
K+R + +
Sbjct: 420 KMRLEFE 426
>P53_CRIGR (O09185) Cellular tumor antigen p53 (Tumor suppressor
p53)
Length = 393
Score = 30.4 bits (67), Expect = 5.0
Identities = 19/61 (31%), Positives = 25/61 (40%)
Query: 187 PYNQHGFNPLFESTTDAEFNRLKSSPPPKFKFLQEAEEKLRRKMQDDNKGINGNEVDNSL 246
P N P + T D E+ LK +FK QE E L K +KG N +S
Sbjct: 309 PTNTSSSPPPKKKTLDGEYFTLKIRGHERFKMFQELNEALELKDAQASKGSEDNGAHSSY 368
Query: 247 I 247
+
Sbjct: 369 L 369
>ICAL_BOVIN (P20811) Calpain inhibitor (Calpastatin)
Length = 705
Score = 30.4 bits (67), Expect = 5.0
Identities = 28/107 (26%), Positives = 43/107 (40%), Gaps = 13/107 (12%)
Query: 141 ESEDGK-CGKDSRGRSLGDLLDVETPYLTPIASPHFFTPMNCSPYCSPYNQHGFNPLFES 199
ES+ K GK +L DL+D + P N + Y + S
Sbjct: 121 ESKPSKPSGKSDMDTALDDLIDT-------LGEPEEMKEDNTT-----YTGPEVSDPMSS 168
Query: 200 TTDAEFNRLKSSPPPKFKFLQEAEEKLRRKMQDDNKGINGNEVDNSL 246
T E + +S+PPPK+K L EE + D K + N+ ++L
Sbjct: 169 TYIEELGKRESTPPPKYKELLNKEEGIAGPPPDSLKPLGPNDAIDAL 215
>HMG4_HUMAN (O15347) High mobility group protein 4 (HMG-4) (High
mobility group protein 2a) (HMG-2a)
Length = 199
Score = 30.4 bits (67), Expect = 5.0
Identities = 26/94 (27%), Positives = 42/94 (44%), Gaps = 6/94 (6%)
Query: 55 FYMFCWK-RPSTSLRQTGFTPPELCSSMRINDNFVHDPQVQ---TSKEFLFKPYGEDVID 110
F++FC + RP G + ++ + N ++D + Q T L + Y +DV
Sbjct: 99 FFLFCSEFRPKIKSTNPGISIGDVAKKLGEMWNNLNDSEKQPYITKAAKLKEKYEKDV-- 156
Query: 111 ADYMMQHHHDGLLGHPRFLFTIVEESKEDLESED 144
ADY + DG G + VEE E+ E E+
Sbjct: 157 ADYKSKGKFDGAKGPAKVARKKVEEEDEEQEEEE 190
>C166_RAT (O35112) CD166 antigen precursor (Activated leukocyte-cell
adhesion molecule) (ALCAM) (MEMD protein) (HB2) (SB-10
antigen) (KG-CAM)
Length = 583
Score = 30.4 bits (67), Expect = 5.0
Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Query: 11 LSIVFGCLLLALVAEV-YYLLWWKKKIIKREIENDYGNGNPLKEL 54
+ IV G LL ALVA V Y+L K K + + D GN K+L
Sbjct: 530 VGIVVGLLLAALVAGVVYWLYMKKSKTASKHVNKDLGNMEENKKL 574
>C166_RABIT (O46651) CD166 antigen (Activated leukocyte-cell
adhesion molecule) (ALCAM) (SB-10 antigen) (Fragment)
Length = 521
Score = 30.4 bits (67), Expect = 5.0
Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Query: 11 LSIVFGCLLLALVAEV-YYLLWWKKKIIKREIENDYGNGNPLKEL 54
+ IV G LL ALVA V Y+L K K + + D GN K+L
Sbjct: 468 VGIVVGLLLAALVAGVVYWLYMKKSKTASKHVNKDLGNMEENKKL 512
>C166_MOUSE (Q61490) CD166 antigen precursor (Activated
leukocyte-cell adhesion molecule) (ALCAM) (DM-GRASP
protein)
Length = 583
Score = 30.4 bits (67), Expect = 5.0
Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Query: 11 LSIVFGCLLLALVAEV-YYLLWWKKKIIKREIENDYGNGNPLKEL 54
+ IV G LL ALVA V Y+L K K + + D GN K+L
Sbjct: 530 VGIVVGLLLAALVAGVVYWLYMKKSKTASKHVNKDLGNMEENKKL 574
>C166_HUMAN (Q13740) CD166 antigen precursor (Activated
leukocyte-cell adhesion molecule) (ALCAM)
Length = 583
Score = 30.4 bits (67), Expect = 5.0
Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Query: 11 LSIVFGCLLLALVAEV-YYLLWWKKKIIKREIENDYGNGNPLKEL 54
+ IV G LL ALVA V Y+L K K + + D GN K+L
Sbjct: 530 VGIVVGLLLAALVAGVVYWLYMKKSKTASKHVNKDLGNMEENKKL 574
>C166_BOVIN (Q9BH13) CD166 antigen precursor (Activated
leukocyte-cell adhesion molecule) (ALCAM)
Length = 583
Score = 30.4 bits (67), Expect = 5.0
Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Query: 11 LSIVFGCLLLALVAEV-YYLLWWKKKIIKREIENDYGNGNPLKEL 54
+ IV G LL ALVA V Y+L K K + + D GN K+L
Sbjct: 530 VGIVVGLLLAALVAGVVYWLYMKKSKTASKHVNKDLGNMEENKKL 574
>UN17_CAEEL (P34711) Vesicular acetylcholine transporter unc-17
(Uncoordinated protein 17)
Length = 532
Score = 30.0 bits (66), Expect = 6.6
Identities = 14/37 (37%), Positives = 24/37 (64%), Gaps = 2/37 (5%)
Query: 242 VDNSLITIIVD--KKYEREVNHHQCHLQQYHSSTSQV 276
+DN L +IV KY R+++++Q + YH+ TSQ+
Sbjct: 43 LDNMLYMVIVPIIPKYLRDIHNYQVTFEGYHNETSQL 79
>DYNA_RAT (P28023) Dynactin 1 (150 kDa dynein-associated
polypeptide) (DP-150) (DAP-150) (p150-glued)
Length = 1280
Score = 30.0 bits (66), Expect = 6.6
Identities = 32/120 (26%), Positives = 53/120 (43%), Gaps = 17/120 (14%)
Query: 151 SRGRSLGDLLDVE--TPYLTPIASPHFFTPMNCSPYCSPYNQHGFNPLFESTTDAEFNRL 208
S S G+L E TP TP+A+P TP SP +P PL + + E R
Sbjct: 170 SGSASAGELSSSEPSTPAQTPLAAPIIPTPALTSPGAAP-------PLPSPSKEEEGLR- 221
Query: 209 KSSPPPKFKFLQEAEEKLRRKMQDDNKGINGNEVDNSLITIIVDKKYEREVNHHQCHLQQ 268
+ + L+E E LR K +D + E++ I + ++++ ++ Q LQ+
Sbjct: 222 -----DQVRDLEEKLETLRLKRSEDKAKL--KELEKHKIQLEQVQEWKSKMQEQQADLQR 274
>DYNA_MOUSE (O08788) Dynactin 1 (150 kDa dynein-associated
polypeptide) (DP-150) (DAP-150) (p150-glued)
Length = 1281
Score = 30.0 bits (66), Expect = 6.6
Identities = 32/120 (26%), Positives = 53/120 (43%), Gaps = 17/120 (14%)
Query: 151 SRGRSLGDLLDVE--TPYLTPIASPHFFTPMNCSPYCSPYNQHGFNPLFESTTDAEFNRL 208
S S G+L E TP TP+A+P TP SP +P PL + + E R
Sbjct: 170 SGSASAGELSSSEPSTPAQTPLAAPIIPTPALTSPGAAP-------PLPSPSKEEEGLR- 221
Query: 209 KSSPPPKFKFLQEAEEKLRRKMQDDNKGINGNEVDNSLITIIVDKKYEREVNHHQCHLQQ 268
+ + L+E E LR K +D + E++ I + ++++ ++ Q LQ+
Sbjct: 222 -----AQVRDLEEKLETLRLKRSEDKAKL--KELEKHKIQLEQVQEWKSKMQEQQADLQR 274
>VT3C_CAPVK (P18388) T3C protein
Length = 131
Score = 29.6 bits (65), Expect = 8.6
Identities = 12/45 (26%), Positives = 26/45 (57%)
Query: 216 FKFLQEAEEKLRRKMQDDNKGINGNEVDNSLITIIVDKKYEREVN 260
F + + +EK++ + ++K I E++N L ++DKK ++N
Sbjct: 40 FDSVLDKDEKIQDNINTNHKPIESKEINNDLPLTVLDKKDTSDIN 84
>NRM1_CHICK (P51027) Natural resistance-associated macrophage
protein 1 (NRAMP 1)
Length = 555
Score = 29.6 bits (65), Expect = 8.6
Identities = 15/24 (62%), Positives = 16/24 (66%)
Query: 8 GLGLSIVFGCLLLALVAEVYYLLW 31
GLG I G LL+A VA V YLLW
Sbjct: 498 GLGYLIPLGLLLVAYVAFVTYLLW 521
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.137 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,605,804
Number of Sequences: 164201
Number of extensions: 1662026
Number of successful extensions: 3898
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 3888
Number of HSP's gapped (non-prelim): 28
length of query: 281
length of database: 59,974,054
effective HSP length: 109
effective length of query: 172
effective length of database: 42,076,145
effective search space: 7237096940
effective search space used: 7237096940
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 65 (29.6 bits)
Medicago: description of AC147536.6