BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147497.9 - phase: 0
(148 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ZMA2_MOUSE (Q9CPW7) Zinc finger matrin type 2 36 0.027
ZMA2_HUMAN (Q96NC0) Zinc finger matrin type 2 36 0.027
SCE3_SCHPO (O14369) Probable RNA-binding protein sce3 35 0.045
YBK9_ENCCU (Q8STZ1) Hypothetical protein ECU11_2090 35 0.059
Y7I5_ENCCU (Q8ST44) Hypothetical protein ECU07_1850/ECU10_0050 35 0.059
IF2_AQUAE (O67825) Translation initiation factor IF-2 33 0.17
HMG4_MOUSE (O54879) High mobility group protein 4 (HMG-4) (High ... 33 0.17
YO14_CAEEL (P34670) Hypothetical zinc finger protein ZK686.4 in ... 33 0.23
P531_HUMAN (Q12888) Tumor suppressor p53-binding protein 1 (p53-... 33 0.23
YQIA_CAEEL (Q09281) Hypothetical protein C45G9.10 in chromosome III 33 0.29
Y5G8_ENCCU (Q8STA9) Hypothetical protein ECU05_1680/ECU11_0050 33 0.29
RS16_BACTN (Q9RQ15) 30S ribosomal protein S16 32 0.38
HS90_CANAL (P46598) Heat shock protein 90 homolog 32 0.38
DD27_MOUSE (Q921N6) Probable ATP-dependent RNA helicase DDX27 (D... 32 0.38
CRTC_DICDI (Q23858) Calreticulin precursor 32 0.38
ATRX_CAEEL (Q9U7E0) Transcriptional regulator ATRX homolog (X-li... 32 0.38
YG49_SCHPO (O60184) Protein C23E6.09 in chromosome II 32 0.65
RPB1_CAEEL (P16356) DNA-directed RNA polymerase II largest subun... 32 0.65
CRTC_CAEEL (P27798) Calreticulin precursor 32 0.65
THO2_HUMAN (Q8NI27) THO complex subunit 2 (Tho2) 31 0.86
>ZMA2_MOUSE (Q9CPW7) Zinc finger matrin type 2
Length = 199
Score = 36.2 bits (82), Expect = 0.027
Identities = 23/95 (24%), Positives = 50/95 (52%), Gaps = 1/95 (1%)
Query: 37 QPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKKAK 96
+ K K I + T S + NV D + K+S +F D+ + +K + +++V+++
Sbjct: 59 ESKLGKTIVITKTTPQSEMGGYYCNVCDCVVKDSINFLDHINGKKHQRNLGMSMRVERST 118
Query: 97 I-TVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEE 130
+ V+++ +V+K ++K D E+ E ++EE
Sbjct: 119 LDQVKKRFEVNKKKMEEKQKDYDFEERMKELREEE 153
>ZMA2_HUMAN (Q96NC0) Zinc finger matrin type 2
Length = 199
Score = 36.2 bits (82), Expect = 0.027
Identities = 23/95 (24%), Positives = 50/95 (52%), Gaps = 1/95 (1%)
Query: 37 QPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKKAK 96
+ K K I + T S + NV D + K+S +F D+ + +K + +++V+++
Sbjct: 59 ESKLGKTIVITKTTPQSEMGGYYCNVCDCVVKDSINFLDHINGKKHQRNLGMSMRVERST 118
Query: 97 I-TVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEE 130
+ V+++ +V+K ++K D E+ E ++EE
Sbjct: 119 LDQVKKRFEVNKKKMEEKQKDYDFEERMKELREEE 153
>SCE3_SCHPO (O14369) Probable RNA-binding protein sce3
Length = 388
Score = 35.4 bits (80), Expect = 0.045
Identities = 31/107 (28%), Positives = 50/107 (45%), Gaps = 12/107 (11%)
Query: 27 VKTQTSSPSYQPKSSKPI--PFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVL 84
V T+ + + SSKP PF P + T+ L+ V + +AK + SF D+A +
Sbjct: 254 VNTEATPSATTTTSSKPKRDPFGGA-KPVDNTSVLQRVEEKLAKRTQSFRREDNANR--- 309
Query: 85 SAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEES 131
+ K + ++ K DK A+AE K D+ + EKK E+
Sbjct: 310 -----ERSTSRKPSADKAEKTDKTDAIAE-KVSDIRLGDGEKKSSET 350
>YBK9_ENCCU (Q8STZ1) Hypothetical protein ECU11_2090
Length = 634
Score = 35.0 bits (79), Expect = 0.059
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 4/70 (5%)
Query: 75 DNDSAEKVVLSAICTVKVKKA----KITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEE 130
D D + V+SA+C+V+ +K + E K K ++ A E+ + V +E EK++E
Sbjct: 304 DKDIEDSDVMSAVCSVRERKRLEEMREMEERKRKEEERAKNEEELLRMVEREEREKREES 363
Query: 131 SGLTAPNQGN 140
G +GN
Sbjct: 364 KGRGKKKRGN 373
>Y7I5_ENCCU (Q8ST44) Hypothetical protein ECU07_1850/ECU10_0050
Length = 634
Score = 35.0 bits (79), Expect = 0.059
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 4/70 (5%)
Query: 75 DNDSAEKVVLSAICTVKVKKA----KITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEE 130
D D + V+SA+C+V+ +K + E K K ++ A E+ + V +E EK++E
Sbjct: 304 DKDIEDSDVMSAVCSVRERKRLEEMREMEERKRKEEERAKNEEELLRMVEREEREKREES 363
Query: 131 SGLTAPNQGN 140
G +GN
Sbjct: 364 KGRGKKKRGN 373
>IF2_AQUAE (O67825) Translation initiation factor IF-2
Length = 805
Score = 33.5 bits (75), Expect = 0.17
Identities = 18/53 (33%), Positives = 31/53 (57%)
Query: 78 SAEKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEE 130
S E+++ + + +K K VE++ K +K V KK + EK++EKK+EE
Sbjct: 127 SVEELIKEILEKKEKEKEKKKVEKERKEEKVRVVEVKKEERKEEKKEEKKEEE 179
>HMG4_MOUSE (O54879) High mobility group protein 4 (HMG-4) (High
mobility group protein 2a) (HMG-2a)
Length = 199
Score = 33.5 bits (75), Expect = 0.17
Identities = 27/92 (29%), Positives = 44/92 (47%), Gaps = 7/92 (7%)
Query: 46 FSSTFDP----SNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKKAKITVEE 101
F S F P +NP + +V + + + DN+ V +A K +K +
Sbjct: 102 FCSEFRPKIKSTNPGISIGDVAKKLGEMWNNLSDNEKQPYVTKAAKLKEKYEKDVADYKS 161
Query: 102 KMKVDKA---AAVAEKKAKDVYEKEDEKKDEE 130
K K D A A VA KK ++ E+E+E+++EE
Sbjct: 162 KGKFDGAKGPAKVARKKVEEEEEEEEEEEEEE 193
>YO14_CAEEL (P34670) Hypothetical zinc finger protein ZK686.4 in
chromosome III
Length = 407
Score = 33.1 bits (74), Expect = 0.23
Identities = 22/94 (23%), Positives = 45/94 (47%), Gaps = 1/94 (1%)
Query: 39 KSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKKAKIT 98
K K + + + F +V D + K+S +F D+ + + + ++K KK+ +
Sbjct: 261 KVGKSVVITKATPSAETGGFYCDVCDCVVKDSINFLDHINGKNHQRNIGMSMKTKKSTVA 320
Query: 99 -VEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEES 131
V E+ K+ K EKK V + ++ ++EE+
Sbjct: 321 DVRERFKLMKDKKEREKKEAQVEQLLEDVQEEEA 354
>P531_HUMAN (Q12888) Tumor suppressor p53-binding protein 1
(p53-binding protein 1) (53BP1)
Length = 1972
Score = 33.1 bits (74), Expect = 0.23
Identities = 15/37 (40%), Positives = 25/37 (67%)
Query: 103 MKVDKAAAVAEKKAKDVYEKEDEKKDEESGLTAPNQG 139
M V+ A AV E+K +++ +KE EK+++ SG T + G
Sbjct: 123 MSVESAPAVEEEKGEELEQKEKEKEEDTSGNTTHSLG 159
>YQIA_CAEEL (Q09281) Hypothetical protein C45G9.10 in chromosome III
Length = 971
Score = 32.7 bits (73), Expect = 0.29
Identities = 25/90 (27%), Positives = 47/90 (51%), Gaps = 14/90 (15%)
Query: 28 KTQTSSPSYQPKSSKPIPFSSTFDPSN--PTAFLKNVFDFIAKESTSF-FDNDSAEKVVL 84
+ QT+SP+Y IP ++++ P+ P+ L N+ DF+ KE + F DS E L
Sbjct: 791 RPQTTSPTY-------IPVTTSYPPTTNPPSPALPNLIDFLNKERENVNFKYDSTE---L 840
Query: 85 SAICTVKV-KKAKITVEEKMKVDKAAAVAE 113
+ IC+ ++ + T+ + V+ +A+
Sbjct: 841 AMICSYEIFENGTTTITRVLPVNGTYTIAK 870
>Y5G8_ENCCU (Q8STA9) Hypothetical protein ECU05_1680/ECU11_0050
Length = 612
Score = 32.7 bits (73), Expect = 0.29
Identities = 21/63 (33%), Positives = 33/63 (52%), Gaps = 5/63 (7%)
Query: 91 KVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESGLT-----APNQGNLMRVI 145
K ++ K EEK + K EKK + EK++EKK+E+SG + A + + V
Sbjct: 340 KKEEKKKKKEEKKEEKKEEKKEEKKEEKKEEKKEEKKEEKSGKSLREGEASEEAEMPSVE 399
Query: 146 IGG 148
+GG
Sbjct: 400 VGG 402
Score = 28.5 bits (62), Expect = 5.5
Identities = 15/40 (37%), Positives = 24/40 (59%)
Query: 91 KVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEE 130
K K+ K +E+ K +K EKK + EK++EKK+E+
Sbjct: 328 KRKEEKKKKKEEKKEEKKKKKEEKKEEKKEEKKEEKKEEK 367
Score = 28.1 bits (61), Expect = 7.2
Identities = 15/38 (39%), Positives = 22/38 (57%)
Query: 93 KKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEE 130
KK K +E+ K K EKK + EK++EKK+E+
Sbjct: 334 KKKKEEKKEEKKKKKEEKKEEKKEEKKEEKKEEKKEEK 371
>RS16_BACTN (Q9RQ15) 30S ribosomal protein S16
Length = 184
Score = 32.3 bits (72), Expect = 0.38
Identities = 17/43 (39%), Positives = 24/43 (55%)
Query: 94 KAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESGLTAP 136
KA++ E+K+ KA A+AEKKA + EK + E AP
Sbjct: 130 KARLEAEKKINEVKAKALAEKKAAEAAEKAAAEAPAEEATEAP 172
>HS90_CANAL (P46598) Heat shock protein 90 homolog
Length = 707
Score = 32.3 bits (72), Expect = 0.38
Identities = 30/118 (25%), Positives = 50/118 (41%), Gaps = 10/118 (8%)
Query: 24 VQTVKTQTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVV 83
VQ + Y +S+ F+ T D +N + KE + + K V
Sbjct: 137 VQVISKHNDDEQYVWESNAGGKFTVTLDETNERLGRGTMLRLFLKEDQLEYLEEKRIKEV 196
Query: 84 LSA--------ICTVKVKKAKITVEEKMKVDKAAAVAEKKAK--DVYEKEDEKKDEES 131
+ I V K+ + V E + DKAA +KK K +V ++EDEKK++++
Sbjct: 197 VKKHSEFVAYPIQLVVTKEVEKEVPETEEEDKAAEEDDKKPKLEEVKDEEDEKKEKKT 254
>DD27_MOUSE (Q921N6) Probable ATP-dependent RNA helicase DDX27
(DEAD-box protein 27)
Length = 760
Score = 32.3 bits (72), Expect = 0.38
Identities = 25/78 (32%), Positives = 38/78 (48%), Gaps = 6/78 (7%)
Query: 64 DFIAKESTSFFDNDSAEKVVLSAICTVKVKKAKITVEEKM-KVDKAAAVAEKKAKD--VY 120
DF+ E +D A V+S + K K+A T++EK+ KV K +K+AK V
Sbjct: 54 DFVFTEKEGMYDGSWALADVMSQL---KKKRAATTLDEKIEKVRKRRKAEDKEAKSGKVE 110
Query: 121 EKEDEKKDEESGLTAPNQ 138
EKE + + G P +
Sbjct: 111 EKEGQADSDLKGQENPGE 128
>CRTC_DICDI (Q23858) Calreticulin precursor
Length = 424
Score = 32.3 bits (72), Expect = 0.38
Identities = 25/83 (30%), Positives = 43/83 (51%), Gaps = 7/83 (8%)
Query: 48 STFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKKAKITVEEKMKVDK 107
S F+ T ++ DF K +F N AEK + + K ++ K +EK+ +K
Sbjct: 326 SIFNNMIVTDSVEEAKDFSEK---TFVANQEAEKKMFDDLEAAKAEERK-KADEKLAAEK 381
Query: 108 AAAVAEKKAKDVYEKEDEKKDEE 130
AA EK+A++ E+E+E +E+
Sbjct: 382 AA---EKEAEEADEEEEEVAEED 401
>ATRX_CAEEL (Q9U7E0) Transcriptional regulator ATRX homolog
(X-linked nuclear protein-1)
Length = 1359
Score = 32.3 bits (72), Expect = 0.38
Identities = 22/70 (31%), Positives = 37/70 (52%), Gaps = 1/70 (1%)
Query: 60 KNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDV 119
KN + K + + ++D EK + +K KKAK E + + +K ++KK+K V
Sbjct: 170 KNKEKSVKKRAETSEESDEDEKPSKKSKKGLK-KKAKSESESESEDEKEVKKSKKKSKKV 228
Query: 120 YEKEDEKKDE 129
+KE E +DE
Sbjct: 229 VKKESESEDE 238
>YG49_SCHPO (O60184) Protein C23E6.09 in chromosome II
Length = 1102
Score = 31.6 bits (70), Expect = 0.65
Identities = 28/118 (23%), Positives = 47/118 (39%), Gaps = 4/118 (3%)
Query: 15 NVFVTKRIIVQTVKTQTSSPSYQPKSSKPIPFSSTFDPSNPTAF-LKNVFDFIAKESTSF 73
N T+ +I TV QT++PS Q + I S+ F P A +N
Sbjct: 775 NNVATQPVIASTVPVQTAAPSSQTAVPQTIHQSNAFTPRGKHASGSRNSISSTKSPQHKL 834
Query: 74 FDNDSAEKVVLSAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEES 131
D + ++I V ++ +V K + + +A E K +++ KK E S
Sbjct: 835 SDQPRSRN---NSISNVSHRERSNSVSSKSRETRTSASNESDPKKSTQRDSSKKLENS 889
Score = 28.5 bits (62), Expect = 5.5
Identities = 29/120 (24%), Positives = 48/120 (39%), Gaps = 13/120 (10%)
Query: 35 SYQPKSSKPIPFSSTFDPSNPTAFLKNVFDFIAKESTSFFDNDSAEKVVLSAICTVKVKK 94
S P SS + + P P LK V +ST ++ + T VK+
Sbjct: 893 SGSPSSSSKSDAAKSIKPQKPEPALKPVEGTADPKSTKRNHQETEKTADTDVSSTEPVKR 952
Query: 95 AKITV------EEKMK------VDKAAAVAEKKAKDVYEKEDEKKDEESG-LTAPNQGNL 141
K EE++K VD A +E K++ + + +EK D+ S +T N ++
Sbjct: 953 QKTADVNDDVGEEEVKQSVSEQVDSAQLTSEPKSESLPKSPEEKSDDTSNDVTTENTNDI 1012
>RPB1_CAEEL (P16356) DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6)
Length = 1852
Score = 31.6 bits (70), Expect = 0.65
Identities = 13/25 (52%), Positives = 17/25 (68%)
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
SSPSY P S + P S T+ P++PT
Sbjct: 1664 SSPSYSPSSPRYSPTSPTYSPTSPT 1688
Score = 28.9 bits (63), Expect = 4.2
Identities = 12/25 (48%), Positives = 16/25 (64%)
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
+SP Y P S + P S T+ PS+PT
Sbjct: 1740 TSPQYSPTSPQYSPSSPTYTPSSPT 1764
Score = 28.5 bits (62), Expect = 5.5
Identities = 12/25 (48%), Positives = 16/25 (64%)
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
+SPSY P S + P S + PS+PT
Sbjct: 1733 TSPSYSPTSPQYSPTSPQYSPSSPT 1757
Score = 28.5 bits (62), Expect = 5.5
Identities = 11/27 (40%), Positives = 16/27 (58%)
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPTAF 58
+SP Y P S P S T++P++P F
Sbjct: 1747 TSPQYSPSSPTYTPSSPTYNPTSPRGF 1773
Score = 28.5 bits (62), Expect = 5.5
Identities = 12/25 (48%), Positives = 15/25 (60%)
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
SSPSY P S P S + P++PT
Sbjct: 1657 SSPSYSPSSPSYSPSSPRYSPTSPT 1681
Score = 28.1 bits (61), Expect = 7.2
Identities = 12/25 (48%), Positives = 15/25 (60%)
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
SSP Y P S P S T+ P++PT
Sbjct: 1671 SSPRYSPTSPTYSPTSPTYSPTSPT 1695
Score = 27.7 bits (60), Expect = 9.5
Identities = 11/25 (44%), Positives = 16/25 (64%)
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
+SP+Y P S P S ++ PS+PT
Sbjct: 1823 TSPTYSPSSPTYSPASPSYSPSSPT 1847
Score = 27.7 bits (60), Expect = 9.5
Identities = 11/25 (44%), Positives = 16/25 (64%)
Query: 32 SSPSYQPKSSKPIPFSSTFDPSNPT 56
+SP+Y P S P S T+ P++PT
Sbjct: 1678 TSPTYSPTSPTYSPTSPTYSPTSPT 1702
>CRTC_CAEEL (P27798) Calreticulin precursor
Length = 395
Score = 31.6 bits (70), Expect = 0.65
Identities = 32/119 (26%), Positives = 53/119 (43%), Gaps = 17/119 (14%)
Query: 30 QTSSPSYQPKSSKPIPFSSTFDPSNPTAFLKNV----FDFIAKESTSFFDN----DSAEK 81
Q +P+Y+ K P + + P + ++ FD +S + FDN DS E+
Sbjct: 274 QIKNPAYKGKWIHPEIENPEYTPDDELYSYESWGAIGFDLWQVKSGTIFDNIIITDSVEE 333
Query: 82 VVLSAICTVKVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKE--------DEKKDEESG 132
A T K + E+K K D+ AE++A+ E+E +E+K+EE G
Sbjct: 334 AEAHAAETFDKLKT-VEKEKKEKADEETRKAEEEARKKAEEEKEAKKDDDEEEKEEEEG 391
>THO2_HUMAN (Q8NI27) THO complex subunit 2 (Tho2)
Length = 1478
Score = 31.2 bits (69), Expect = 0.86
Identities = 15/47 (31%), Positives = 25/47 (52%)
Query: 91 KVKKAKITVEEKMKVDKAAAVAEKKAKDVYEKEDEKKDEESGLTAPN 137
K K+ + +EK + K K K+ ++KE++ KDE+ T PN
Sbjct: 1187 KPKEERPNKDEKARETKERTPKSDKEKEKFKKEEKAKDEKFKTTVPN 1233
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.311 0.126 0.336
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,804,228
Number of Sequences: 164201
Number of extensions: 594630
Number of successful extensions: 4191
Number of sequences better than 10.0: 114
Number of HSP's better than 10.0 without gapping: 49
Number of HSP's successfully gapped in prelim test: 66
Number of HSP's that attempted gapping in prelim test: 3740
Number of HSP's gapped (non-prelim): 472
length of query: 148
length of database: 59,974,054
effective HSP length: 100
effective length of query: 48
effective length of database: 43,553,954
effective search space: 2090589792
effective search space used: 2090589792
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC147497.9