BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147496.7 + phase: 0
(110 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MAC4_HUMAN (Q96PK2) Microtubule-actin crosslinking factor 1, iso... 31 0.59
Y587_PSEAE (Q9I5V0) Hypothetical UPF0229 protein PA0587 30 0.77
KLC_STRPU (Q05090) Kinesin light chain (KLC) 30 0.77
GPD2_YEAST (P41911) Glycerol-3-phosphate dehydrogenase [NAD+] 2 ... 30 1.3
YE63_THETN (Q8R9W9) Hypothetical UPF0122 protein TTE1463 29 1.7
UVH1_ARATH (Q9LKI5) DNA repair endonuclease UVH1 (EC 3.1.-.-) (U... 29 1.7
PDX1_PYRAE (Q8ZUF0) Pyridoxine biosynthesis protein pdx1 28 2.9
Y396_PSEPK (P59351) Hypothetical UPF0229 protein PP0396 28 3.8
TFT1_BOVIN (P27628) Tuftelin (Enamelin) 28 3.8
METE_PYRHO (O58816) Probable methylcobalamin:homocysteine methyl... 28 3.8
DLPA_LEGPN (Q48806) Protein dlpA 28 3.8
CC15_YEAST (P27636) Cell division control protein 15 (EC 2.7.1.-) 28 3.8
YH14_CLOPE (Q8XJP2) Hypothetical UPF0122 protein CPE1714 28 5.0
UCP3_PIG (O97649) Mitochondrial uncoupling protein 3 (UCP 3) 28 5.0
DNAK_ACTAC (P71331) Chaperone protein dnaK (Heat shock protein 7... 28 5.0
UCP2_RAT (P56500) Mitochondrial uncoupling protein 2 (UCP 2) 27 6.5
RPSB_ANASP (Q03065) RNA polymerase sigma-B factor 27 6.5
DNAK_BORBR (Q7WGI4) Chaperone protein dnaK (Heat shock protein 7... 27 6.5
THRC_SERMA (P27735) Threonine synthase (EC 4.2.3.1) 27 8.5
SCRR_STAXY (P74892) Sucrose operon repressor (Scr operon regulat... 27 8.5
>MAC4_HUMAN (Q96PK2) Microtubule-actin crosslinking factor 1, isoform
4
Length = 5938
Score = 30.8 bits (68), Expect = 0.59
Identities = 20/80 (25%), Positives = 37/80 (46%), Gaps = 10/80 (12%)
Query: 14 GGGVKAVKEVVTTAKNVVAAVKEIVNCTEQEIY-----DVLRE-----CDMDPNLAVEKL 63
G +E T +++ +K+ +C + E +V+ E C+ L E++
Sbjct: 1343 GNDTNEEQEKAVTKIEIISHMKQSTSCLDSEEIRENQGEVILEVQETYCETSGKLPSEQV 1402
Query: 64 LSQDTFREVRSKREKRKEVI 83
L Q V+SKREKR+ ++
Sbjct: 1403 LQQPMNARVKSKREKREVIV 1422
>Y587_PSEAE (Q9I5V0) Hypothetical UPF0229 protein PA0587
Length = 423
Score = 30.4 bits (67), Expect = 0.77
Identities = 23/69 (33%), Positives = 32/69 (46%), Gaps = 6/69 (8%)
Query: 7 VNRNSGGGGGVKAVKEVVTTAKNVVAAVKEIV-NCTEQEIYDVLRECDMDPNLAVEKLLS 65
+ R SGGGGG K A N + + V T++E D + E PNL +
Sbjct: 88 IARPSGGGGGRGGGK-----ASNSGEGMDDFVFQITQEEFLDFMFEDLELPNLVKRHITG 142
Query: 66 QDTFREVRS 74
DTF+ VR+
Sbjct: 143 TDTFKTVRA 151
>KLC_STRPU (Q05090) Kinesin light chain (KLC)
Length = 686
Score = 30.4 bits (67), Expect = 0.77
Identities = 18/68 (26%), Positives = 34/68 (49%), Gaps = 7/68 (10%)
Query: 2 GSESVVNRNSGGGGGVKAVKEVVTTAKNVVAAVK-------EIVNCTEQEIYDVLRECDM 54
GS+ NSGGG G + ++++T + V+ ++ +I+N Q + + ++
Sbjct: 3 GSKLSTPNNSGGGQGNLSQEQIITGTREVIKGLEQLKNEHNDILNSLYQSLKMLKKDTPG 62
Query: 55 DPNLAVEK 62
D NL EK
Sbjct: 63 DSNLVEEK 70
>GPD2_YEAST (P41911) Glycerol-3-phosphate dehydrogenase [NAD+] 2 (EC
1.1.1.8)
Length = 440
Score = 29.6 bits (65), Expect = 1.3
Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 7/68 (10%)
Query: 13 GGGGVKAVKEVVTTAKNVVAAVKEIVN-------CTEQEIYDVLRECDMDPNLAVEKLLS 65
GG VK + T K+ + A KE++N T +E+++ L+ C++ + + +
Sbjct: 357 GGRNVKVATYMAKTGKSALEAEKELLNGQSAQGIITCREVHEWLQTCELTQEFPLFEAVY 416
Query: 66 QDTFREVR 73
Q + VR
Sbjct: 417 QIVYNNVR 424
>YE63_THETN (Q8R9W9) Hypothetical UPF0122 protein TTE1463
Length = 117
Score = 29.3 bits (64), Expect = 1.7
Identities = 17/52 (32%), Positives = 26/52 (49%)
Query: 34 VKEIVNCTEQEIYDVLRECDMDPNLAVEKLLSQDTFREVRSKREKRKEVILL 85
+ EI++ + Q +YD L+ + EKL +E+ K EK KE I L
Sbjct: 40 ISEILDISRQGVYDALKRAEDSLEFYEEKLGLVKKHQEMMKKIEKIKECIKL 91
>UVH1_ARATH (Q9LKI5) DNA repair endonuclease UVH1 (EC 3.1.-.-)
(Ultraviolet hypersensitive 1) (AtRAD1) (DNA excision
repair protein XP-F homolog)
Length = 956
Score = 29.3 bits (64), Expect = 1.7
Identities = 19/95 (20%), Positives = 46/95 (48%), Gaps = 3/95 (3%)
Query: 3 SESVVNRNSGGGGGVKAVKEVVTTAKNVVAAVKEIVNCTEQEIYDVLRECD---MDPNLA 59
+++ + R +GG ++ +V+ + ++++ +++ +I V E + P++
Sbjct: 706 TQNSLTRKAGGRKELEKETQVIVDMREFMSSLPNVLHQKGMKIIPVTLEVGDYILSPSIC 765
Query: 60 VEKLLSQDTFREVRSKREKRKEVILLRYVNIDMRL 94
VE+ QD F+ S R + ++ RY I + L
Sbjct: 766 VERKSIQDLFQSFTSGRLFHQVEMMSRYYRIPVLL 800
>PDX1_PYRAE (Q8ZUF0) Pyridoxine biosynthesis protein pdx1
Length = 336
Score = 28.5 bits (62), Expect = 2.9
Identities = 15/52 (28%), Positives = 23/52 (43%)
Query: 3 SESVVNRNSGGGGGVKAVKEVVTTAKNVVAAVKEIVNCTEQEIYDVLRECDM 54
SE S G G V E V K++ A+K + E+ + D R+C +
Sbjct: 180 SEGASMIRSKGEAGTGNVAEAVKHFKSIYGAIKSLAAGDEERVRDFARQCQV 231
>Y396_PSEPK (P59351) Hypothetical UPF0229 protein PP0396
Length = 423
Score = 28.1 bits (61), Expect = 3.8
Identities = 23/69 (33%), Positives = 31/69 (44%), Gaps = 6/69 (8%)
Query: 7 VNRNSGGGGGVKAVKEVVTTAKNVVAAVKEIV-NCTEQEIYDVLRECDMDPNLAVEKLLS 65
+ R GGGGG K A N + E V T++E + + E PNL L
Sbjct: 88 IPRPQGGGGGGGRGK-----AGNSGEGMDEFVFQITQEEFLEFMFEDLELPNLVKRHLTG 142
Query: 66 QDTFREVRS 74
DTF+ VR+
Sbjct: 143 ADTFKTVRA 151
>TFT1_BOVIN (P27628) Tuftelin (Enamelin)
Length = 390
Score = 28.1 bits (61), Expect = 3.8
Identities = 17/62 (27%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
Query: 2 GSESVVNRNSGGGGGVKAVKEV-VTTAKNVVAAVKEIVNCTEQEIYDVLRECDMDPNLAV 60
G E ++ G G K + E + K+ V ++E NC ++ D+ + D DP +V
Sbjct: 73 GHEEIIKVYLKGRSGDKMIHEKNINQLKSEVQYIQEARNCLQKLREDISSKLDRDPGDSV 132
Query: 61 EK 62
K
Sbjct: 133 HK 134
>METE_PYRHO (O58816) Probable methylcobalamin:homocysteine
methyltransferase (EC 2.1.1.-) (Methionine synthase)
Length = 338
Score = 28.1 bits (61), Expect = 3.8
Identities = 18/71 (25%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Query: 13 GGGGVKAVKEVVTTAKNVVAAVKEIVNCTEQEIYDVLRECDM---DPNLAVEKLLSQDTF 69
G G V + + + +V A+K++ E E+ + +C M D N+A KL++
Sbjct: 255 GFGVVDVHNPRIESVEEIVKAIKKVFEYVEPELLYINPDCGMKLLDRNIAYNKLVNMVKA 314
Query: 70 REVRSKREKRK 80
E+ K +R+
Sbjct: 315 TELVRKELERE 325
>DLPA_LEGPN (Q48806) Protein dlpA
Length = 615
Score = 28.1 bits (61), Expect = 3.8
Identities = 14/46 (30%), Positives = 24/46 (51%)
Query: 21 KEVVTTAKNVVAAVKEIVNCTEQEIYDVLRECDMDPNLAVEKLLSQ 66
KE + + + +++++NC EI D L C ++ L K LSQ
Sbjct: 383 KENLIESDTMPNLLQQLINCNSAEISDALDACGIEGGLLSIKPLSQ 428
>CC15_YEAST (P27636) Cell division control protein 15 (EC 2.7.1.-)
Length = 974
Score = 28.1 bits (61), Expect = 3.8
Identities = 19/75 (25%), Positives = 34/75 (45%), Gaps = 5/75 (6%)
Query: 13 GGGGVKAVKEVVTTAKNVVAAVKEIVNCTEQEIYDVLRECDMDPNLAVEKLLSQDTFREV 72
G G V + + + V A+KE+V ++E+ D++ E + NL ++ F
Sbjct: 32 GRGSYGVVYKAINKHTDQVVAIKEVVYENDEELNDIMAEISLLKNLNHNNIVKYHGF--- 88
Query: 73 RSKREKRKEVILLRY 87
R+ + ILL Y
Sbjct: 89 --IRKSYELYILLEY 101
>YH14_CLOPE (Q8XJP2) Hypothetical UPF0122 protein CPE1714
Length = 112
Score = 27.7 bits (60), Expect = 5.0
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Query: 31 VAAVKEIVNCTEQEIYDVLRECDMDPNLAVEKL-LSQDTFREVRSKREKRKEV 82
+A + EI + Q IYD+++ C + EKL LS+ + R K E E+
Sbjct: 36 LAEIAEINKTSRQAIYDLIKRCSKQLHSYDEKLKLSKKVDKRYRIKEELMAEL 88
>UCP3_PIG (O97649) Mitochondrial uncoupling protein 3 (UCP 3)
Length = 308
Score = 27.7 bits (60), Expect = 5.0
Identities = 12/27 (44%), Positives = 17/27 (62%)
Query: 37 IVNCTEQEIYDVLRECDMDPNLAVEKL 63
IVNC E YDV++E +D +L + L
Sbjct: 187 IVNCAEMVTYDVIKEKVLDYHLLTDNL 213
>DNAK_ACTAC (P71331) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 632
Score = 27.7 bits (60), Expect = 5.0
Identities = 18/61 (29%), Positives = 33/61 (53%), Gaps = 2/61 (3%)
Query: 16 GVKAVKEVVTTAKNVVAAVKEIVNCTEQEIYDVLRECDMDPNLAVEKLLSQDTFREVRSK 75
G A ++ VT KN + A+K ++ E +V R+ D+ P A+ K + D + EV+ +
Sbjct: 50 GQPAKRQAVTNPKNTLFAIKRLIG-RRFEDEEVKRDIDIMP-FAITKADNGDAWVEVKGE 107
Query: 76 R 76
+
Sbjct: 108 K 108
>UCP2_RAT (P56500) Mitochondrial uncoupling protein 2 (UCP 2)
Length = 309
Score = 27.3 bits (59), Expect = 6.5
Identities = 15/38 (39%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Query: 26 TAKNVVAAVKEIVNCTEQEIYDVLRECDMDPNLAVEKL 63
T+ NV A IVNCTE YD++++ + NL + L
Sbjct: 179 TSPNV--ARNAIVNCTELVTYDLIKDTLLKANLMTDDL 214
>RPSB_ANASP (Q03065) RNA polymerase sigma-B factor
Length = 332
Score = 27.3 bits (59), Expect = 6.5
Identities = 17/69 (24%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Query: 20 VKEVVTTAKNVVAAVKEIVNCTEQEIYDVLRECDMDPNLAVEK-LLSQDTFREVRSKREK 78
++E + + V+ I + ++ D+L++ M P E+ LL QD + +
Sbjct: 216 IREYLQLVRQPVSLDMRIGFEQDTQLQDLLKDDGMSPERYAERELLYQDIHNLLAKLTPQ 275
Query: 79 RKEVILLRY 87
+KEV++LR+
Sbjct: 276 QKEVLILRF 284
>DNAK_BORBR (Q7WGI4) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 641
Score = 27.3 bits (59), Expect = 6.5
Identities = 19/61 (31%), Positives = 32/61 (52%), Gaps = 2/61 (3%)
Query: 16 GVKAVKEVVTTAKNVVAAVKEIVNCTEQEIYDVLRECDMDPNLAVEKLLSQDTFREVRSK 75
G A ++ VT KN + AVK ++ E V ++ D+ P ++ K + D + EVR K
Sbjct: 51 GAPAKRQAVTNPKNTLYAVKRLIGRKFDE-KAVQKDIDLMP-YSIVKADNGDAWVEVRGK 108
Query: 76 R 76
+
Sbjct: 109 K 109
>THRC_SERMA (P27735) Threonine synthase (EC 4.2.3.1)
Length = 429
Score = 26.9 bits (58), Expect = 8.5
Identities = 22/94 (23%), Positives = 44/94 (46%), Gaps = 13/94 (13%)
Query: 16 GVKAVKEVVTTAKNVVAAVKEIVNCT-EQEIYDVLRECDMDPNLAVEKLLSQDTFREVRS 74
G+K V+ V+ + ++ ++E + CT I+ V + D D A+ K D
Sbjct: 147 GLKNVRVVILYPQGKISPLQEKLFCTLGGNIHTVAIDGDFDACQALVKQAFDD------- 199
Query: 75 KREKRKEVILLRYVNIDMRLNLSLMMNNVCMYFD 108
++ K+ + L N +N+S ++ +C YF+
Sbjct: 200 --QELKDALHLNSAN---SINISRLLAQICYYFE 228
>SCRR_STAXY (P74892) Sucrose operon repressor (Scr operon regulatory
protein)
Length = 320
Score = 26.9 bits (58), Expect = 8.5
Identities = 15/77 (19%), Positives = 37/77 (47%), Gaps = 18/77 (23%)
Query: 41 TEQEIYDVLRECDMDPNLAVEKLLSQ------------------DTFREVRSKREKRKEV 82
T+Q++ +++RE D PN + L ++ +T + V++ ++
Sbjct: 31 TQQKLDEIIRENDYQPNQFAQSLRARRTNMIGAIIPRMNSFAVDETIKGVKTVCDQLNYS 90
Query: 83 ILLRYVNIDMRLNLSLM 99
+LL Y N++++L + +
Sbjct: 91 LLLNYTNLNIQLEIDAL 107
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.135 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,270,178
Number of Sequences: 164201
Number of extensions: 385545
Number of successful extensions: 1702
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 20
Number of HSP's that attempted gapping in prelim test: 1693
Number of HSP's gapped (non-prelim): 29
length of query: 110
length of database: 59,974,054
effective HSP length: 86
effective length of query: 24
effective length of database: 45,852,768
effective search space: 1100466432
effective search space used: 1100466432
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147496.7