BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147484.9 - phase: 0
(125 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TP3B_HUMAN (O95985) DNA topoisomerase III beta-1 (EC 5.99.1.2) 96 1e-20
TP3B_MOUSE (Q9Z321) DNA topoisomerase III beta-1 (EC 5.99.1.2) 94 5e-20
TP3B_DROME (O96651) DNA topoisomerase III beta (EC 5.99.1.2) 85 2e-17
TP3A_HUMAN (Q13472) DNA topoisomerase III alpha (EC 5.99.1.2) 59 1e-09
TP3A_MOUSE (O70157) DNA topoisomerase III alpha (EC 5.99.1.2) 58 4e-09
TP3A_DROME (Q9NG98) DNA topoisomerase III alpha (EC 5.99.1.2) 55 2e-08
TOP3_SCHPO (O60126) DNA topoisomerase III (EC 5.99.1.2) 51 4e-07
TOP3_CAEEL (O61660) DNA topoisomerase III (EC 5.99.1.2) 47 6e-06
TOP1_THEAC (Q9HM08) DNA topoisomerase I (EC 5.99.1.2) (Omega-pro... 44 6e-05
TOP3_YEAST (P13099) DNA topoisomerase III (EC 5.99.1.2) 43 1e-04
TOP1_ARCFU (O28469) DNA topoisomerase I (EC 5.99.1.2) (Omega-pro... 35 0.029
TOP1_METJA (Q59046) DNA topoisomerase I (EC 5.99.1.2) (Omega-pro... 31 0.56
Y4ED_RHISN (P55427) Hypothetical 42.2 kDa protein y4eD 30 0.73
VP1_BPPH6 (P11126) P1 protein 29 1.6
TOP3_PASMU (Q9CP53) DNA topoisomerase III (EC 5.99.1.2) 29 2.1
MYS1_YEAST (P08964) Myosin-1 (Type II myosin) 28 3.6
BACH_HALSS (P33742) Halorhodopsin (HR) 28 4.7
TOP3_HAEIN (P43704) DNA topoisomerase III (EC 5.99.1.2) 27 8.0
TOP1_PYRFU (O73954) DNA topoisomerase I (EC 5.99.1.2) (Omega-pro... 27 8.0
TOP1_PYRAB (Q9UYS8) DNA topoisomerase I (EC 5.99.1.2) (Omega-pro... 27 8.0
>TP3B_HUMAN (O95985) DNA topoisomerase III beta-1 (EC 5.99.1.2)
Length = 862
Score = 96.3 bits (238), Expect = 1e-20
Identities = 50/93 (53%), Positives = 63/93 (66%), Gaps = 4/93 (4%)
Query: 7 VLMVAEKPSIALSIATVLSHGKMNTRRG---STEVHEFDGKFRKAPARFKVTSVIGHVFS 63
VLMVAEKPS+A SIA +LS G +++ +G + VHE+ G F P RFK+TSV GHV +
Sbjct: 4 VLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMT 63
Query: 64 VDFPGKYQDWAATDPLDLF-QAQVIKNESNPKL 95
+DF GKY W DP +LF QA K E+NPKL
Sbjct: 64 LDFLGKYNKWDKVDPAELFSQAPTEKKEANPKL 96
>TP3B_MOUSE (Q9Z321) DNA topoisomerase III beta-1 (EC 5.99.1.2)
Length = 862
Score = 94.0 bits (232), Expect = 5e-20
Identities = 49/93 (52%), Positives = 62/93 (65%), Gaps = 4/93 (4%)
Query: 7 VLMVAEKPSIALSIATVLSHGKMNTRRG---STEVHEFDGKFRKAPARFKVTSVIGHVFS 63
VLMVAEKPS+A SIA +LS G M++ +G + VH++ G F P FK+TSV GHV +
Sbjct: 4 VLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVMT 63
Query: 64 VDFPGKYQDWAATDPLDLF-QAQVIKNESNPKL 95
+DF GKY W DP +LF QA K E+NPKL
Sbjct: 64 LDFLGKYNKWDKVDPAELFSQAPTEKKEANPKL 96
>TP3B_DROME (O96651) DNA topoisomerase III beta (EC 5.99.1.2)
Length = 875
Score = 85.1 bits (209), Expect = 2e-17
Identities = 44/95 (46%), Positives = 59/95 (61%), Gaps = 4/95 (4%)
Query: 4 LPKVLMVAEKPSIALSIATVLSHGKMNTRRGS---TEVHEFDGKFR-KAPARFKVTSVIG 59
+ VLMVAEKPS+A S+A +LS+G+ +RG+ HE+ G FR + F++TSV G
Sbjct: 1 MKSVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNFRNEGSVHFRMTSVCG 60
Query: 60 HVFSVDFPGKYQDWAATDPLDLFQAQVIKNESNPK 94
HV S+DF KY W DP+ LF K E+NPK
Sbjct: 61 HVMSLDFNKKYNCWDKVDPIQLFGCATEKKETNPK 95
>TP3A_HUMAN (Q13472) DNA topoisomerase III alpha (EC 5.99.1.2)
Length = 1001
Score = 59.3 bits (142), Expect = 1e-09
Identities = 33/86 (38%), Positives = 51/86 (58%), Gaps = 3/86 (3%)
Query: 6 KVLMVAEKPSIALSIATVLSHGKMNTRRGSTE---VHEFDGKFRKAPARFKVTSVIGHVF 62
KVL VAEK A IA +LS+G+M R G ++ ++EFD +TSV GH+
Sbjct: 35 KVLCVAEKNDAAKGIADLLSNGRMRRREGLSKFNKIYEFDYHLYGQNVTMVMTSVSGHLL 94
Query: 63 SVDFPGKYQDWAATDPLDLFQAQVIK 88
+ DF +++ W + +PL LF+A++ K
Sbjct: 95 AHDFQMQFRKWQSCNPLVLFEAEIEK 120
>TP3A_MOUSE (O70157) DNA topoisomerase III alpha (EC 5.99.1.2)
Length = 1003
Score = 57.8 bits (138), Expect = 4e-09
Identities = 32/86 (37%), Positives = 51/86 (59%), Gaps = 3/86 (3%)
Query: 6 KVLMVAEKPSIALSIATVLSHGKMNTRRGSTE---VHEFDGKFRKAPARFKVTSVIGHVF 62
KVL VAEK A IA +LS+G+M + G ++ ++EFD +TSV GH+
Sbjct: 35 KVLCVAEKNDAAKGIADLLSNGRMRRKEGLSKFNKIYEFDYHLYGQNVTMIMTSVSGHLL 94
Query: 63 SVDFPGKYQDWAATDPLDLFQAQVIK 88
+ DF +++ W + +PL LF+A++ K
Sbjct: 95 AHDFQMQFRKWQSCNPLVLFEAEIEK 120
>TP3A_DROME (Q9NG98) DNA topoisomerase III alpha (EC 5.99.1.2)
Length = 1250
Score = 55.5 bits (132), Expect = 2e-08
Identities = 33/86 (38%), Positives = 46/86 (53%), Gaps = 3/86 (3%)
Query: 6 KVLMVAEKPSIALSIATVLSHGKMNTRRGST---EVHEFDGKFRKAPARFKVTSVIGHVF 62
K L VAEK A +IA +LS+G R G + +V +F+ R A+ +TSV GH+
Sbjct: 27 KYLNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPVRGQNAKMVMTSVSGHMM 86
Query: 63 SVDFPGKYQDWAATDPLDLFQAQVIK 88
+ F Y++W DP LF A V K
Sbjct: 87 QLAFQVSYKNWRTVDPRSLFDAPVKK 112
>TOP3_SCHPO (O60126) DNA topoisomerase III (EC 5.99.1.2)
Length = 622
Score = 51.2 bits (121), Expect = 4e-07
Identities = 32/91 (35%), Positives = 49/91 (53%), Gaps = 5/91 (5%)
Query: 6 KVLMVAEKPSIALSIATVLSHG---KMNTRRGSTEVHEFDGKF--RKAPARFKVTSVIGH 60
+VL VAEK SIA S+A++L G + +TR + ++F F + +TSV GH
Sbjct: 2 RVLCVAEKNSIAKSVASILGGGHVRRRDTRSKYVKNYDFSFNFGGNVGSSDVTMTSVSGH 61
Query: 61 VFSVDFPGKYQDWAATDPLDLFQAQVIKNES 91
+ FP +Y W++ LF AQ+I + S
Sbjct: 62 LTEASFPSEYSSWSSVPQDVLFDAQIITSVS 92
>TOP3_CAEEL (O61660) DNA topoisomerase III (EC 5.99.1.2)
Length = 759
Score = 47.4 bits (111), Expect = 6e-06
Identities = 25/86 (29%), Positives = 43/86 (49%), Gaps = 3/86 (3%)
Query: 4 LPKVLMVAEKPSIALSIATVLSHGKMNTRRGST---EVHEFDGKFRKAPARFKVTSVIGH 60
+ + L VAEK +A +A +LS+G N R G + +++ + + VTSV GH
Sbjct: 1 MKRALFVAEKNDVAKGVAAILSNGTANRREGRSKFNKIYTLNTELFGQQTAISVTSVSGH 60
Query: 61 VFSVDFPGKYQDWAATDPLDLFQAQV 86
+ + F +W ++LF+A V
Sbjct: 61 MMNFQFHENMSNWQTASMVELFRAPV 86
>TOP1_THEAC (Q9HM08) DNA topoisomerase I (EC 5.99.1.2)
(Omega-protein) (Relaxing enzyme) (Untwisting enzyme)
(Swivelase)
Length = 770
Score = 43.9 bits (102), Expect = 6e-05
Identities = 27/86 (31%), Positives = 40/86 (46%), Gaps = 5/86 (5%)
Query: 8 LMVAEKPSIALSIATVLSHGKMNTRRGS-TEVHEFDGKFRKAPARFKVTSVIGHVFSVDF 66
+++AEK IA LS G++ + R T EF+ K + + GH+ DF
Sbjct: 6 IIIAEKADAGRRIAYFLSGGQVKSHRAKGTSYLEFEYNGSKT----YLIPLSGHIVEADF 61
Query: 67 PGKYQDWAATDPLDLFQAQVIKNESN 92
Y DW D DL A+++KN N
Sbjct: 62 ESGYSDWNKIDLSDLIDARIVKNIKN 87
>TOP3_YEAST (P13099) DNA topoisomerase III (EC 5.99.1.2)
Length = 656
Score = 42.7 bits (99), Expect = 1e-04
Identities = 32/100 (32%), Positives = 46/100 (46%), Gaps = 10/100 (10%)
Query: 6 KVLMVAEKPSIALSIATVLSHGKMNTR-RGSTEVHEFDGKFRKAP-------ARFKVTSV 57
KVL VAEK SIA +++ +L G+ +R G V +D F P +TSV
Sbjct: 2 KVLCVAEKNSIAKAVSQILGGGRSTSRDSGYMYVKNYDFMFSGFPFARNGANCEVTMTSV 61
Query: 58 IGHVFSVDFPGKYQDWAATDPLDLFQAQV--IKNESNPKL 95
GH+ +DF W +LF A + I N + K+
Sbjct: 62 AGHLTGIDFSHDSHGWGKCAIQELFDAPLNEIMNNNQKKI 101
>TOP1_ARCFU (O28469) DNA topoisomerase I (EC 5.99.1.2)
(Omega-protein) (Relaxing enzyme) (Untwisting enzyme)
(Swivelase)
Length = 663
Score = 35.0 bits (79), Expect = 0.029
Identities = 21/82 (25%), Positives = 39/82 (46%), Gaps = 8/82 (9%)
Query: 8 LMVAEKPSIALSIATVLSHGKMNTRRGSTEVHEFDGKFRKAPARFK-VTSVIGHVFSVDF 66
L++ EK + A IA++L ++G + +P+ V + GH+ +DF
Sbjct: 6 LIITEKDNTARRIASILFKDVKTLKKGRVSYYH-------SPSNDAYVVGLKGHIVELDF 58
Query: 67 PGKYQDWAATDPLDLFQAQVIK 88
P + +W T L QA+++K
Sbjct: 59 PKELNNWTKTPLEKLLQAELVK 80
>TOP1_METJA (Q59046) DNA topoisomerase I (EC 5.99.1.2)
(Omega-protein) (Relaxing enzyme) (Untwisting enzyme)
(Swivelase)
Length = 761
Score = 30.8 bits (68), Expect = 0.56
Identities = 20/58 (34%), Positives = 29/58 (49%), Gaps = 7/58 (12%)
Query: 8 LMVAEKPSIALSIATVLSHGKMNTRRGSTEVH-EFDGKFRKAPARFKVTSVIGHVFSV 64
L++ EKPS+A IA L K + G E DGK + V S +GH+F++
Sbjct: 8 LIICEKPSVAKKIANALGKAKKKSIDGVPYYELERDGK------KIIVASAVGHLFTL 59
>Y4ED_RHISN (P55427) Hypothetical 42.2 kDa protein y4eD
Length = 375
Score = 30.4 bits (67), Expect = 0.73
Identities = 15/45 (33%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Query: 59 GHVFSVDFPGKYQDWAATDPLDLFQ--AQVIKNESNPKLVDVVIW 101
G+V +D G D TDP++L Q ++ + NPKL V++
Sbjct: 164 GYVMIIDVKGAVDDQDPTDPIELTQRCLDILAAKKNPKLSKAVVF 208
>VP1_BPPH6 (P11126) P1 protein
Length = 769
Score = 29.3 bits (64), Expect = 1.6
Identities = 15/52 (28%), Positives = 24/52 (45%), Gaps = 6/52 (11%)
Query: 53 KVTSVIGHVFSVDFPGK------YQDWAATDPLDLFQAQVIKNESNPKLVDV 98
+ TS IG ++D G+ Y+DW + F + N SN + +DV
Sbjct: 338 ETTSYIGQTSAIDHMGQPSHVVVYEDWQFAKEITAFTPVKLANNSNQRFLDV 389
>TOP3_PASMU (Q9CP53) DNA topoisomerase III (EC 5.99.1.2)
Length = 650
Score = 28.9 bits (63), Expect = 2.1
Identities = 22/63 (34%), Positives = 28/63 (43%), Gaps = 11/63 (17%)
Query: 8 LMVAEKPSIALSIATVLSHGKMNTRRGSTEVHEFDGKFRKAPARFKVTSVIGHVFSVDFP 67
L +AEKPS+A +IA VL + H+ F K A VT IGH+ P
Sbjct: 3 LFIAEKPSLARAIADVL-----------PKPHQRGDGFIKCGADDYVTWCIGHLLEQAEP 51
Query: 68 GKY 70
Y
Sbjct: 52 DAY 54
>MYS1_YEAST (P08964) Myosin-1 (Type II myosin)
Length = 1928
Score = 28.1 bits (61), Expect = 3.6
Identities = 18/74 (24%), Positives = 32/74 (42%), Gaps = 1/74 (1%)
Query: 28 KMNTRRGSTEVHEFDGKFRKAPARFKVTSVIGHVF-SVDFPGKYQDWAATDPLDLFQAQV 86
K+ V + K + P R + +G + + D KY++ L+ ++ Q
Sbjct: 1368 KLKFEASEERVKSLEDKLKTMPLRDRTNLPVGDIIKNRDSISKYEEEIRYYKLENYKLQE 1427
Query: 87 IKNESNPKLVDVVI 100
I NESN KL + +
Sbjct: 1428 ILNESNGKLSQLTL 1441
>BACH_HALSS (P33742) Halorhodopsin (HR)
Length = 284
Score = 27.7 bits (60), Expect = 4.7
Identities = 16/62 (25%), Positives = 29/62 (45%), Gaps = 5/62 (8%)
Query: 68 GKYQDWAATDPLDLFQAQVIKNESNPKLVDVVIWYCGWIVT-----VKEKIYVLRVIFVS 122
G+Y WA + P+ L ++ + KL V+ G VT + Y+LR ++ +
Sbjct: 112 GRYLTWALSTPMILIAVGLLAGSNTTKLFTAVVADIGMCVTGLAAALTTSSYLLRWVWYA 171
Query: 123 LS 124
+S
Sbjct: 172 IS 173
>TOP3_HAEIN (P43704) DNA topoisomerase III (EC 5.99.1.2)
Length = 651
Score = 26.9 bits (58), Expect = 8.0
Identities = 20/63 (31%), Positives = 27/63 (42%), Gaps = 11/63 (17%)
Query: 8 LMVAEKPSIALSIATVLSHGKMNTRRGSTEVHEFDGKFRKAPARFKVTSVIGHVFSVDFP 67
L +AEKPS+A +IA VL + H+ F K VT +GH+ P
Sbjct: 3 LFIAEKPSLARAIADVL-----------PKPHQRGDGFIKCGDNDVVTWCVGHLLEQAEP 51
Query: 68 GKY 70
Y
Sbjct: 52 DAY 54
>TOP1_PYRFU (O73954) DNA topoisomerase I (EC 5.99.1.2)
(Omega-protein) (Relaxing enzyme) (Untwisting enzyme)
(Swivelase) [Contains: Endonuclease PI-PfuI (EC
3.1.-.-) (Pfu topA intein)]
Length = 1060
Score = 26.9 bits (58), Expect = 8.0
Identities = 19/61 (31%), Positives = 31/61 (50%), Gaps = 9/61 (14%)
Query: 7 VLMVAEKPSIALSIATVLSHGK-MNTRRGSTEVHEF--DGKFRKAPARFKVTSVIGHVFS 63
+L++AEKP++A IA L+ K + +E DGK R V S +GH++
Sbjct: 2 ILVIAEKPNVARKIAMALAEVKPIKKTLFGVPYYELIRDGK------RLIVASAVGHLYG 55
Query: 64 V 64
+
Sbjct: 56 L 56
>TOP1_PYRAB (Q9UYS8) DNA topoisomerase I (EC 5.99.1.2)
(Omega-protein) (Relaxing enzyme) (Untwisting enzyme)
(Swivelase)
Length = 685
Score = 26.9 bits (58), Expect = 8.0
Identities = 20/61 (32%), Positives = 31/61 (50%), Gaps = 9/61 (14%)
Query: 7 VLMVAEKPSIALSIATVLSHGK--MNTRRGSTEVHEF-DGKFRKAPARFKVTSVIGHVFS 63
+L++AEKP++A IA LS + T G F DGK + V S +GH++
Sbjct: 2 ILVIAEKPNVARKIAGALSERRPIRKTIFGVPYYEVFRDGK------KLIVASAVGHLYG 55
Query: 64 V 64
+
Sbjct: 56 L 56
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.137 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,647,098
Number of Sequences: 164201
Number of extensions: 497964
Number of successful extensions: 1044
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1023
Number of HSP's gapped (non-prelim): 22
length of query: 125
length of database: 59,974,054
effective HSP length: 101
effective length of query: 24
effective length of database: 43,389,753
effective search space: 1041354072
effective search space used: 1041354072
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147484.9