BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC147484.3 - phase: 0 /pseudo
         (526 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BRNQ_HAEIN (P71345) Branched-chain amino acid transport system c...    35  0.62
BP28_HUMAN (Q9H583) Protein BAP28                                      33  1.4
DNLI_FOWPV (Q67480) DNA ligase (EC 6.5.1.1) (Polydeoxyribonucleo...    32  5.3
YL32_ARCFU (O28148) Hypothetical protein AF2132                        31  6.9
T2BF_BACSU (P25217) Type II restriction enzyme BsuFI (EC 3.1.21....    31  6.9
BRNQ_ECOLI (P37011) Branched-chain amino acid transport system I...    31  6.9
ACVS_CEPAC (P25464) N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D...    31  6.9
TOLB_CHLTR (O84604) TolB protein precursor                             31  9.0

>BRNQ_HAEIN (P71345) Branched-chain amino acid transport system
           carrier protein (Branched-chain amino acid uptake
           carrier)
          Length = 436

 Score = 34.7 bits (78), Expect = 0.62
 Identities = 17/72 (23%), Positives = 32/72 (43%)

Query: 336 LFEILRLSVPFHPVQYETLKLIYECISECPGAVSTSQMEELVLVLIKMLTKHSDGEMGMI 395
           L + LR  +P     Y +  L+  C S C    +   + E +  L+K     S+G   ++
Sbjct: 356 LLQFLRKKLPSIKFTYNSTLLVTVCFSLCDSLNNVKMLPESINSLLKHFPLSSEGMAWLV 415

Query: 396 PETFIMACSVFV 407
           P   ++  S+F+
Sbjct: 416 PTLVMLVASIFI 427


>BP28_HUMAN (Q9H583) Protein BAP28
          Length = 2144

 Score = 33.5 bits (75), Expect = 1.4
 Identities = 74/330 (22%), Positives = 132/330 (39%), Gaps = 42/330 (12%)

Query: 72  LHSIVTFHPHFLVSPLVAALSSFDDEPIAEQLVHLILNLSASSDPS-------VCR---- 120
           L  ++ F+   +VS LVAA     D  IA+   ++   L +S           +C+    
Sbjct: 213 LRVLLAFYASTIVSALVAA-EDVSDNIIAKLFPYIQKGLKSSLPDYRAATYMIICQISVK 271

Query: 121 -----EFVARVSDRVSSGALGWSSRQLHSLHCLGVLLNCEKEDDL----HVHIKDVYSLI 171
                 FV  ++ ++        S     L CL VLL  +K + L      H+ +V  LI
Sbjct: 272 VTMENTFVNSLASQIIKTLTKIPSLIKDGLSCLIVLLQRQKPESLGKKPFPHLCNVPDLI 331

Query: 172 SVLVTGLQLPSEEIRGEVLFVLYKLFAL---HSTSDEGDGSDMLIPFCPKLLYLLGDVLL 228
           ++L  G+   + ++   + ++L  L      H T +E +G D  I +   L  +L  + L
Sbjct: 332 TIL-HGIS-ETYDVSPLLRYMLPHLVVSIIHHVTGEETEGMDGQI-YKRHLEAILTKISL 388

Query: 229 KTQNDDVRLNCIALLTMLAQRQLLREEPAYDTGNMSLSGEVNFKETEDVPEGASLVNLFA 288
           K   D        LL  L    L  E  +Y +     S +V+    + +P    L + + 
Sbjct: 389 KNNLDH-------LLASL----LFEEYISYSSQEEMDSNKVSLLNEQFLPLIRLLESKYP 437

Query: 289 EAIKGPLLSSVSQVQIGALDLLFHYLSSVGTSGNQIQVLVEENIADYLFEILRLSVPFHP 348
             +   L   + ++       LFH   S+ TSG + Q L + + +     +L L+ P  P
Sbjct: 438 RTLDVVLEEHLKEIADLKKQELFHQFVSLSTSGGKYQFLADSDTS----LMLSLNHPLAP 493

Query: 349 VQYETLKLIYECISECPGAVSTSQMEELVL 378
           V+   +  + + +      V  S ++E VL
Sbjct: 494 VRILAMNHLKKIMKTSKEGVDESFIKEAVL 523


>DNLI_FOWPV (Q67480) DNA ligase (EC 6.5.1.1)
           (Polydeoxyribonucleotide synthase [ATP])
          Length = 564

 Score = 31.6 bits (70), Expect = 5.3
 Identities = 11/47 (23%), Positives = 24/47 (50%)

Query: 322 NQIQVLVEENIADYLFEILRLSVPFHPVQYETLKLIYECISECPGAV 368
           N ++ +V+ ++ D +  ++ L VP  P+     K   E + +CP  +
Sbjct: 185 NNLEQVVQRSLEDNIKPLIELMVPLQPMLASACKTFSEAVKKCPNGI 231


>YL32_ARCFU (O28148) Hypothetical protein AF2132
          Length = 244

 Score = 31.2 bits (69), Expect = 6.9
 Identities = 14/33 (42%), Positives = 21/33 (63%)

Query: 310 LFHYLSSVGTSGNQIQVLVEENIADYLFEILRL 342
           L  +L   G S  Q+ V +E+NIAD+++E  RL
Sbjct: 55  LSRFLKQHGYSLRQVHVALEQNIADFIYEFQRL 87


>T2BF_BACSU (P25217) Type II restriction enzyme BsuFI (EC 3.1.21.4)
           (Endonuclease BsuFI) (R.BsuFI)
          Length = 395

 Score = 31.2 bits (69), Expect = 6.9
 Identities = 20/66 (30%), Positives = 33/66 (49%), Gaps = 5/66 (7%)

Query: 44  LISNPSSPTIHVSYALS----QLSRSLSIPQFLHSIVTFHPHFLVSPLVAALSSFDDEPI 99
           L+SN   P   VS  +     +L R++SI       VT H    VS L++ L   + +P+
Sbjct: 227 LLSNRGKPKTDVSVTIKTNTKELIRNISIKNTREKTVTIHEGS-VSDLISRLKLSETDPL 285

Query: 100 AEQLVH 105
           ++ L+H
Sbjct: 286 SQALIH 291


>BRNQ_ECOLI (P37011) Branched-chain amino acid transport system II
           carrier protein (LIV-II)
          Length = 439

 Score = 31.2 bits (69), Expect = 6.9
 Identities = 14/51 (27%), Positives = 27/51 (52%)

Query: 35  TICLVCFSNLISNPSSPTIHVSYALSQLSRSLSIPQFLHSIVTFHPHFLVS 85
           T+C +    L + P + T+     ++ L+   ++P F++S+V F    LVS
Sbjct: 87  TVCYLAVGPLFATPRTATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVS 137


>ACVS_CEPAC (P25464)
            N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine
            synthase (EC 6.3.2.26)
            (Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine
            synthetase) (ACV synthetase) (ACVS)
          Length = 3712

 Score = 31.2 bits (69), Expect = 6.9
 Identities = 21/73 (28%), Positives = 39/73 (52%), Gaps = 6/73 (8%)

Query: 420  DLSKSIEEAVKQAILACLYVS----ERNINQILQCFYLLKEAYAYSHDENSTHISKLELR 475
            DL+ +++E+V    +   Y +    E  +   ++ F+LL    A  H++ ST +SKL + 
Sbjct: 2358 DLNVTVKESVNSLNVNFNYPTSLFEEETVQGFMETFHLLLRQLA--HNKASTSLSKLSVE 2415

Query: 476  SGILDICRTHLLP 488
             G+L+   T+L P
Sbjct: 2416 DGVLNPEPTNLQP 2428


>TOLB_CHLTR (O84604) TolB protein precursor
          Length = 431

 Score = 30.8 bits (68), Expect = 9.0
 Identities = 22/61 (36%), Positives = 31/61 (50%), Gaps = 7/61 (11%)

Query: 142 LHSLHCLGVLL----NCEKEDDLHVHIKDVYSLISVLVTGLQLPSEEIRGEVLFVLYKLF 197
           L SL CL  LL    +CE   DL +H++   SL+ + V+ L  P +  +   L  L  LF
Sbjct: 8   LRSLLCLLCLLPSTLHCE---DLEIHVRSESSLLPIAVSLLSSPKDSRQASYLASLRDLF 64

Query: 198 A 198
           A
Sbjct: 65  A 65


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.321    0.137    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,713,678
Number of Sequences: 164201
Number of extensions: 2446683
Number of successful extensions: 5160
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 5154
Number of HSP's gapped (non-prelim): 12
length of query: 526
length of database: 59,974,054
effective HSP length: 115
effective length of query: 411
effective length of database: 41,090,939
effective search space: 16888375929
effective search space used: 16888375929
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 68 (30.8 bits)