BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147482.3 - phase: 0
(175 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MAVI_CUCPE (P80728) Mavicyanin 62 7e-10
ENL2_ARATH (Q9T076) Early nodulin-like protein 2 precursor (Phyt... 60 3e-09
ENL1_ARATH (Q9SK27) Early nodulin-like protein 1 precursor (Phyt... 57 3e-08
STEL_RHUVE (P00302) Stellacyanin 56 4e-08
BABL_CUCSA (P00303) Basic blue protein (Cusacyanin) (Plantacyani... 56 4e-08
BCP_PEA (Q41001) Blue copper protein precursor 55 8e-08
N552_SOYBN (Q02917) Early nodulin 55-2 precursor (N-55-2) (Nodul... 54 1e-07
UMEC_ARMRU (P42849) Umecyanin (UMC) 50 2e-06
CPC_CUCSA (P29602) Cucumber peeling cupredoxin (CPC) (Stellacyanin) 49 6e-06
NO16_MEDTR (P93328) Early nodulin 16 precursor (N-16) 49 8e-06
BCB2_ARATH (O80517) Uclacyanin II precursor (Blue copper-binding... 46 4e-05
BABL_ARATH (Q8LG89) Putative basic blue protein precursor (Plant... 46 4e-05
NO20_MEDTR (P93329) Early nodulin 20 precursor (N-20) 45 1e-04
BCB1_ARATH (Q07488) Blue copper protein precursor (Blue copper-b... 45 1e-04
N551_SOYBN (Q05544) Early nodulin 55-1 (N-55-1) (Fragment) 44 2e-04
ENL3_ARATH (Q8LC95) Early nodulin-like protein 3 precursor (Phyt... 44 2e-04
BABL_LILLO (P60496) Chemocyanin precursor (Basic blue protein) (... 43 4e-04
NUKM_MOUSE (Q9DC70) NADH-ubiquinone oxidoreductase 20 kDa subuni... 30 3.7
MFGM_MOUSE (P21956) Lactadherin precursor (Milk fat globule-EGF ... 29 6.3
CLYT_CLYGR (Q08121) Clytin precursor (Phialidin) 29 6.3
>MAVI_CUCPE (P80728) Mavicyanin
Length = 108
Score = 62.0 bits (149), Expect = 7e-10
Identities = 35/100 (35%), Positives = 55/100 (55%), Gaps = 4/100 (4%)
Query: 30 LHKVGGSKGWKENV--NYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTF 87
+HKVG S GW V +Y W+S +VGD L F ++ +++NVL+V++ + C +
Sbjct: 3 VHKVGDSTGWTTLVPYDYAKWASSNKFHVGDSLLFNYNNKFHNVLQVDQEQFKSCNSSSP 62
Query: 88 IRNLTRGGRDVVQLTEAKTYYFITG-GGYCFHGMKVAVDV 126
+ T G D + L T+YF+ G G+C G KV + V
Sbjct: 63 AASYT-SGADSIPLKRPGTFYFLCGIPGHCQLGQKVEIKV 101
>ENL2_ARATH (Q9T076) Early nodulin-like protein 2 precursor
(Phytocyanin-like protein)
Length = 349
Score = 60.1 bits (144), Expect = 3e-09
Identities = 46/141 (32%), Positives = 64/141 (44%), Gaps = 6/141 (4%)
Query: 16 VLSLWPMVVMGGPKLHKVGGSKGWKENV--NYTTWSSQEHVYVGDWLKFVFDKRYYNVLE 73
+LSL + + + VGGS W N NY +WS + V D L F + K +VLE
Sbjct: 16 LLSLSTLFTISNARKFNVGGSGAWVTNPPENYESWSGKNRFLVHDTLYFSYAKGADSVLE 75
Query: 74 VNKTGYDYCIDMTFIRNLTRGGRDVVQLTEAKTYYFITGG-GYCFHGMKVAVDV--QEHP 130
VNK YD C I+ + G + + L +YFI+G C G K+ V V P
Sbjct: 76 VNKADYDACNTKNPIKRVDDGDSE-ISLDRYGPFYFISGNEDNCKKGQKLNVVVISARIP 134
Query: 131 TPAPSPSLSDTAKSGGDSILP 151
+ A SP + S S+ P
Sbjct: 135 STAQSPHAAAPGSSTPGSMTP 155
>ENL1_ARATH (Q9SK27) Early nodulin-like protein 1 precursor
(Phytocyanin-like protein)
Length = 176
Score = 56.6 bits (135), Expect = 3e-08
Identities = 44/146 (30%), Positives = 70/146 (47%), Gaps = 9/146 (6%)
Query: 33 VGGSKG-WK----ENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTF 87
VGG G WK + ++T W+ + VGD++ F ++ +VLEV K Y+ C
Sbjct: 27 VGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIVFRYESGKDSVLEVTKEAYNSCNTTNP 86
Query: 88 IRNLTRGGRDVVQLTEAKTYYFITG-GGYCFHGMKVAVDVQEHPTPAPSPSLSDTAKSGG 146
+ N T G V+L + +YFI+G G+C G K+++ V SP+ S G
Sbjct: 87 LANYT-DGETKVKLDRSGPFYFISGANGHCEKGQKLSLVVISPRHSVISPAPSPVEFEDG 145
Query: 147 DSILPSMYTCFGIIVANVVYVSLVLV 172
++ P+ + G + YV L LV
Sbjct: 146 PALAPAPIS--GSVRLGGCYVVLGLV 169
>STEL_RHUVE (P00302) Stellacyanin
Length = 107
Score = 56.2 bits (134), Expect = 4e-08
Identities = 33/102 (32%), Positives = 53/102 (51%), Gaps = 5/102 (4%)
Query: 30 LHKVGGSKGWKE----NVNYT-TWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCID 84
++ VG S GWK +V+Y W+S + ++GD L F +D+R++NV +V + Y C D
Sbjct: 2 VYTVGDSAGWKVPFFGDVDYDWKWASNKTFHIGDVLVFKYDRRFHNVDKVTQKNYQSCND 61
Query: 85 MTFIRNLTRGGRDVVQLTEAKTYYFITGGGYCFHGMKVAVDV 126
T I + G + T + YY +C G KV ++V
Sbjct: 62 TTPIASYNTGBBRINLKTVGQKYYICGVPKHCDLGQKVHINV 103
>BABL_CUCSA (P00303) Basic blue protein (Cusacyanin) (Plantacyanin)
(CBP)
Length = 96
Score = 56.2 bits (134), Expect = 4e-08
Identities = 31/96 (32%), Positives = 51/96 (52%), Gaps = 3/96 (3%)
Query: 30 LHKVGGSKGWKENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIR 89
++ VGGS GW N +W + GD L F ++ +NV+ VN+ G+ C +
Sbjct: 2 VYVVGGSGGWTFNTE--SWPKGKRFRAGDILLFNYNPXMHNVVVVNQGGFSTCNTPAGAK 59
Query: 90 NLTRGGRDVVQLTEAKTYYFITGGGYCFHGMKVAVD 125
T GRD ++L + ++Y+ G+C GMK+AV+
Sbjct: 60 VYT-SGRDQIKLPKGQSYFICNFPGHCQSGMKIAVN 94
>BCP_PEA (Q41001) Blue copper protein precursor
Length = 189
Score = 55.1 bits (131), Expect = 8e-08
Identities = 37/136 (27%), Positives = 67/136 (49%), Gaps = 2/136 (1%)
Query: 12 VVLGVLSLWPMVVMGGPKLHKVGGSKGWKENVNYTTWSSQEHVYVGDWLKFVFDKRYYNV 71
V+ +L++ M + ++ VG + GW +Y+TW+S + VGD L F + + V
Sbjct: 8 VLCFLLAIINMALPSLATVYTVGDTSGWVIGGDYSTWASDKTFAVGDSLVFNYGAGAHTV 67
Query: 72 LEVNKTGYDYCIDMTFIRNLTRGGRDVVQLTEAKTYYFITG-GGYCFHGMKVAVDVQEHP 130
EV ++ Y C I + G + L +A +YFI G G+ GMK+++ V+
Sbjct: 68 DEVKESDYKSCTSGNSI-STDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSIKVKASS 126
Query: 131 TPAPSPSLSDTAKSGG 146
+ +PS + ++ G
Sbjct: 127 GSSAAPSATPSSSGKG 142
>N552_SOYBN (Q02917) Early nodulin 55-2 precursor (N-55-2)
(Nodulin-315)
Length = 187
Score = 54.3 bits (129), Expect = 1e-07
Identities = 42/141 (29%), Positives = 70/141 (48%), Gaps = 15/141 (10%)
Query: 11 SVVLGVLSLWPMVVMGGPKLHKVGGS-KGWKENVN----YTTWSSQEHVYVGDWLKFVFD 65
S L +L++ ++ + + VGGS K WK ++ + W++ +GD L F ++
Sbjct: 9 SPFLVMLAMCLLISTSEAEKYVVGGSEKSWKFPLSKPDSLSHWANSHRFKIGDTLIFKYE 68
Query: 66 KRYYNVLEVNKTGYDYCIDMTFIRNLTRGGRDVVQLTEAKTYYFITGG-GYCFHGMKVAV 124
KR +V E N+T Y+ C + + GG V LT+ +FI+G +C G+K+AV
Sbjct: 69 KRTESVHEGNETDYEGCNTVGKYHIVFNGGNTKVMLTKPGFRHFISGNQSHCQMGLKLAV 128
Query: 125 DVQE---------HPTPAPSP 136
V P+P+PSP
Sbjct: 129 LVISSNKTKKNLLSPSPSPSP 149
>UMEC_ARMRU (P42849) Umecyanin (UMC)
Length = 115
Score = 50.4 bits (119), Expect = 2e-06
Identities = 30/99 (30%), Positives = 48/99 (48%), Gaps = 3/99 (3%)
Query: 31 HKVGGSKGWKENVN---YTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTF 87
+ VGG WK + Y TW++ + VGD L+F F ++V V K +D C
Sbjct: 3 YDVGGDMEWKRPSDPKFYITWATGKTFRVGDELEFDFAAGMHDVAVVTKDAFDNCKKENP 62
Query: 88 IRNLTRGGRDVVQLTEAKTYYFITGGGYCFHGMKVAVDV 126
I ++T ++ T YY T G +C G K++++V
Sbjct: 63 ISHMTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSINV 101
>CPC_CUCSA (P29602) Cucumber peeling cupredoxin (CPC) (Stellacyanin)
Length = 137
Score = 48.9 bits (115), Expect = 6e-06
Identities = 35/121 (28%), Positives = 60/121 (48%), Gaps = 11/121 (9%)
Query: 30 LHKVGGSKGWKENVN---YTTWSSQEHVYVGDWLKFVFDKRYYNVLEV-NKTGYDYCIDM 85
+H VG + GW + Y+ W++ + VGD L+F F +NV E+ K +D C +
Sbjct: 4 VHIVGDNTGWSVPSSPNFYSQWAAGKTFRVGDSLQFNFPANAHNVHEMETKQSFDACNFV 63
Query: 86 TFIRNLTRGGRDVVQLTEAKTYYFI-TGGGYCFHGMKVAVDV------QEHPTPAPSPSL 138
++ R + +L E +YF+ T G +C +G K++++V P P+ SP
Sbjct: 64 NSDNDVERTSPVIERLDELGMHYFVCTVGTHCSNGQKLSINVVAANATVSMPPPSSSPPS 123
Query: 139 S 139
S
Sbjct: 124 S 124
>NO16_MEDTR (P93328) Early nodulin 16 precursor (N-16)
Length = 180
Score = 48.5 bits (114), Expect = 8e-06
Identities = 45/163 (27%), Positives = 74/163 (44%), Gaps = 26/163 (15%)
Query: 3 NSRVLLMVSVVLGVLSLWPMVVMGGPKLHKVGGS-KGWKENVN----YTTWSSQEHVYVG 57
+S +LLM+ + S+W ++ + +G S WK + + W+S VG
Sbjct: 4 SSPILLMI-----IFSMWLLISHSESTDYLIGDSHNSWKVPLPSRRAFARWASAHEFTVG 58
Query: 58 DWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIRNLT-RGGRDVVQLTEAKTYYFITG-GGY 115
D + F +D +V EVN+ +DY + T ++ G V L + Y+FI+G +
Sbjct: 59 DTILFEYDNETESVHEVNE--HDYIMCHTNGEHVEHHDGNTKVVLDKIGVYHFISGTKRH 116
Query: 116 CFHGMKVAVDVQEH------------PTPAPSPSLSDTAKSGG 146
C G+K+AV VQ P+PSPS + + GG
Sbjct: 117 CKMGLKLAVVVQNKHDLVLPPLITMPMPPSPSPSPNSSGNKGG 159
>BCB2_ARATH (O80517) Uclacyanin II precursor (Blue copper-binding
protein II) (BCB II) (Phytocyanin 2)
Length = 202
Score = 46.2 bits (108), Expect = 4e-05
Identities = 36/121 (29%), Positives = 56/121 (45%), Gaps = 14/121 (11%)
Query: 39 WKENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIRNLTRGGRDV 98
W V+Y+ W++ + VGD L+F + + V V+K GYD C + N + G +
Sbjct: 36 WTTGVDYSGWATGKTFRVGDILEFKYGSS-HTVDVVDKAGYDGCDASSSTENHSDGDTKI 94
Query: 99 VQLTEAKTYYFITGGGYC--FHGMKVAVDV-------QEHPTPAPS----PSLSDTAKSG 145
T Y+ + G+C GMK+AV+V PTP S P+ ++ SG
Sbjct: 95 DLKTVGINYFICSTPGHCRTNGGMKLAVNVVAGSAGPPATPTPPSSTPGTPTTPESPPSG 154
Query: 146 G 146
G
Sbjct: 155 G 155
>BABL_ARATH (Q8LG89) Putative basic blue protein precursor
(Plantacyanin)
Length = 129
Score = 46.2 bits (108), Expect = 4e-05
Identities = 27/94 (28%), Positives = 46/94 (48%), Gaps = 3/94 (3%)
Query: 31 HKVGGSKGWKENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIRN 90
+ VG S W N W +H GD L F ++ R +NV++V+ Y+ C T +
Sbjct: 36 YTVGDSGIW--TFNAVGWPKGKHFRAGDVLVFNYNPRMHNVVKVDSGSYNNCKTPTGAKP 93
Query: 91 LTRGGRDVVQLTEAKTYYFITGGGYCFHGMKVAV 124
T G+D + L++ + ++ +C MK+AV
Sbjct: 94 YT-SGKDRITLSKGQNFFICNFPNHCESDMKIAV 126
>NO20_MEDTR (P93329) Early nodulin 20 precursor (N-20)
Length = 268
Score = 44.7 bits (104), Expect = 1e-04
Identities = 42/146 (28%), Positives = 68/146 (45%), Gaps = 19/146 (13%)
Query: 3 NSRVLLMVSVVLGVLSLWPMVVMGGPKLHKVGGSKG-WKENVN----YTTWSSQEHVYVG 57
+S +LLM + S+W ++ + VG S+ WK + T W+S VG
Sbjct: 4 SSPILLMF-----IFSIWMLISYSESTDYLVGDSENSWKFPLPTRHALTRWASNYQFIVG 58
Query: 58 DWLKFVFDKRYYNVLEVNKTGYDYC-IDMTFIRNLTRGGRDVVQLTEAKTYYFITG-GGY 115
D + F ++ + +V EV + YD C I + + G +V L + ++FI+G +
Sbjct: 59 DTITFQYNNKTESVHEVEEEDYDRCGIRGEHVDHY--DGNTMVVLKKTGIHHFISGKKRH 116
Query: 116 CFHGMKVAV-----DVQEHPTPAPSP 136
C G+K+AV V P P PSP
Sbjct: 117 CRLGLKLAVVVMVAPVLSSPPPPPSP 142
>BCB1_ARATH (Q07488) Blue copper protein precursor (Blue
copper-binding protein) (AtBCB) (Stellacyanin)
(Phytocyanin 1)
Length = 196
Score = 44.7 bits (104), Expect = 1e-04
Identities = 31/126 (24%), Positives = 56/126 (43%), Gaps = 10/126 (7%)
Query: 31 HKVGGSKGWKENVN---YTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTF 87
+ VG W ++ YTTW++ + VGD L+F F ++V V++ ++ C
Sbjct: 25 YDVGDDTEWTRPMDPEFYTTWATGKTFRVGDELEFDFAAGRHDVAVVSEAAFENCEKEKP 84
Query: 88 IRNLTRGGRDVVQLTEAKTYYFITGGGYCFHGMKVAVDV-------QEHPTPAPSPSLSD 140
I ++T ++ T Y+ T G +C G K+++ V P +P+
Sbjct: 85 ISHMTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLSITVVAAGATGGATPGAGATPAPGS 144
Query: 141 TAKSGG 146
T +GG
Sbjct: 145 TPSTGG 150
>N551_SOYBN (Q05544) Early nodulin 55-1 (N-55-1) (Fragment)
Length = 137
Score = 43.9 bits (102), Expect = 2e-04
Identities = 33/110 (30%), Positives = 51/110 (46%), Gaps = 21/110 (19%)
Query: 64 FDKRYYNVLEVNKTGYDYCIDMTFIRNLTRGGRDVVQLTEAKTYYFITGG-GYCFHGMKV 122
+D+R +V EVN+T Y+ C + L G V LT++ +FI+G +C G+K+
Sbjct: 2 YDERTESVHEVNETDYEQCNTVGKEHVLFNDGNTKVMLTKSGFRHFISGNQSHCQMGLKL 61
Query: 123 AVDVQEH--------------------PTPAPSPSLSDTAKSGGDSILPS 152
V V + P+P+PSPS S + S S LP+
Sbjct: 62 MVVVMSNNTKKKLIHSPSPSSPSPSPSPSPSPSPSPSPSLSSPSPSPLPN 111
>ENL3_ARATH (Q8LC95) Early nodulin-like protein 3 precursor
(Phytocyanin-like protein)
Length = 186
Score = 43.5 bits (101), Expect = 2e-04
Identities = 36/125 (28%), Positives = 54/125 (42%), Gaps = 23/125 (18%)
Query: 34 GGSKGWK------ENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTF 87
G + WK E++N W+ VGD L + +D+ +VL+V K Y C
Sbjct: 30 GKTSSWKIPSSPSESLN--KWAESLRFRVGDTLVWKYDEEKDSVLQVTKDAYINCNTTNP 87
Query: 88 IRNLTRGGRDVVQLTEAKTYYFITGG-GYCFHGMKVAVDVQ-------------EHPTPA 133
N + G V+L + Y+FI+G C G K+ + V P+PA
Sbjct: 88 AANYSNGDTK-VKLERSGPYFFISGSKSNCVEGEKLHIVVMSSRGGHTGGFFTGSSPSPA 146
Query: 134 PSPSL 138
PSP+L
Sbjct: 147 PSPAL 151
>BABL_LILLO (P60496) Chemocyanin precursor (Basic blue protein)
(Plantacyanin)
Length = 126
Score = 42.7 bits (99), Expect = 4e-04
Identities = 26/95 (27%), Positives = 44/95 (45%), Gaps = 3/95 (3%)
Query: 30 LHKVGGSKGWKENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYDYCIDMTFIR 89
++ VG GW + W + + GD L F ++ +NV+ V GY C R
Sbjct: 32 VYTVGDGGGW--TFGTSGWPAGKTFRAGDVLVFKYNPAVHNVVSVPAGGYKSCTASPGSR 89
Query: 90 NLTRGGRDVVQLTEAKTYYFITGGGYCFHGMKVAV 124
+ + G D + L+ Y+ + G+C G+K+AV
Sbjct: 90 -VFKSGDDRITLSRGTNYFICSVPGHCQGGLKIAV 123
>NUKM_MOUSE (Q9DC70) NADH-ubiquinone oxidoreductase 20 kDa subunit,
mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3)
(Complex I-20KD) (CI-20KD) (PSST subunit)
Length = 224
Score = 29.6 bits (65), Expect = 3.7
Identities = 13/30 (43%), Positives = 21/30 (69%), Gaps = 4/30 (13%)
Query: 128 EHPTPAPSPSLSDT----AKSGGDSILPSM 153
E P+P+PSPSLS T +K+G +++P +
Sbjct: 34 EGPSPSPSPSLSSTQSAVSKAGAGAVVPKL 63
>MFGM_MOUSE (P21956) Lactadherin precursor (Milk fat globule-EGF
factor 8) (MFG-E8) (MFGM) (Sperm surface protein SP47)
(MP47)
Length = 463
Score = 28.9 bits (63), Expect = 6.3
Identities = 17/42 (40%), Positives = 21/42 (49%), Gaps = 3/42 (7%)
Query: 103 EAKTYYFITGGGYCFHGMKVAVDVQEHPTPAPSPSLSDTAKS 144
E +T Y+ G Y F AV PTPAP+P LS+ S
Sbjct: 108 ETETNYYNLDGEYMF---TTAVPNTAVPTPAPTPDLSNNLAS 146
>CLYT_CLYGR (Q08121) Clytin precursor (Phialidin)
Length = 198
Score = 28.9 bits (63), Expect = 6.3
Identities = 13/30 (43%), Positives = 16/30 (53%), Gaps = 2/30 (6%)
Query: 38 GWKENVNYTT--WSSQEHVYVGDWLKFVFD 65
GWKE NY WS + + DW + VFD
Sbjct: 94 GWKELANYDLKLWSQNKKSLIRDWGEAVFD 123
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.140 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,446,276
Number of Sequences: 164201
Number of extensions: 972275
Number of successful extensions: 2335
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 2304
Number of HSP's gapped (non-prelim): 23
length of query: 175
length of database: 59,974,054
effective HSP length: 103
effective length of query: 72
effective length of database: 43,061,351
effective search space: 3100417272
effective search space used: 3100417272
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC147482.3