BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147429.11 - phase: 0
(149 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PPA1_LYCES (P27061) Acid phosphatase precursor 1 (EC 3.1.3.2) 40 0.002
SPEE_SULTO (Q975S5) Probable spermidine synthase (EC 2.5.1.16) (... 34 0.13
PUR2_LACLA (Q9ZF44) Phosphoribosylamine--glycine ligase (EC 6.3.... 33 0.30
DEGP_BARHE (P54925) Probable periplasmic serine protease DO-like... 32 0.67
DDLA_ECOLI (P23844) D-alanine--D-alanine ligase A (EC 6.3.2.4) (... 30 1.5
DDLA_ECOL6 (Q8FKE3) D-alanine--D-alanine ligase A (EC 6.3.2.4) (... 30 1.5
DLTA_STRPN (Q97N82) D-alanine--poly(phosphoribitol) ligase subun... 30 1.9
RS4E_METAC (Q8TRT5) 30S ribosomal protein S4e 30 2.5
PANE_SCHPO (Q9HDU6) Probable 2-dehydropantoate 2-reductase (EC 1... 30 2.5
MR11_SULAC (Q8NKQ0) DNA double-strand break repair protein mre11 30 2.5
YF91_MYCTU (O06599) Hypothetical protein Rv1591/MT1626/Mb1617 29 3.3
ORP3_MOUSE (Q9DBS9) Oxysterol binding protein-related protein 3 ... 29 3.3
YOJ8_YEAST (Q12496) Hypothetical 118.4 kDa protein in WRS1-PKH2 ... 29 4.3
DLTA_STRA5 (P59591) D-alanine--poly(phosphoribitol) ligase subun... 29 4.3
DLTA_STRA3 (Q8VM67) D-alanine--poly(phosphoribitol) ligase subun... 29 4.3
DPOL_BPT5 (P19822) DNA polymerase (EC 2.7.7.7) 28 5.7
YVBV_BACSU (O32256) Hypothetical transport protein yvbV 28 7.4
S1A1_MOUSE (Q9QXZ6) Solute carrier organic anion transporter fam... 28 7.4
MYCT_RAT (Q921A2) Proton myo-inositol cotransporter (H(+)-myo-in... 28 7.4
ORP3_HUMAN (Q9H4L5) Oxysterol binding protein-related protein 3 ... 28 9.7
>PPA1_LYCES (P27061) Acid phosphatase precursor 1 (EC 3.1.3.2)
Length = 255
Score = 39.7 bits (91), Expect = 0.002
Identities = 22/81 (27%), Positives = 34/81 (41%), Gaps = 1/81 (1%)
Query: 66 CKIHSLHAKLNNLEE-HNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSEDG 124
C + NNL +P C D +Y+ G Y ++D +Y V +DG
Sbjct: 43 CTTWRFVVETNNLSPWKTIPEECADYVKEYMVGPGYKMEIDRVSDEAGEYAKSVDLGDDG 102
Query: 125 FDVVLIDIDSLFQWNPPHSSN 145
DV + D+D N P+ S+
Sbjct: 103 RDVWIFDVDETLLSNLPYYSD 123
>SPEE_SULTO (Q975S5) Probable spermidine synthase (EC 2.5.1.16)
(Putrescine aminopropyltransferase) (SPDSY)
Length = 300
Score = 33.9 bits (76), Expect = 0.13
Identities = 20/70 (28%), Positives = 38/70 (53%), Gaps = 1/70 (1%)
Query: 72 HAKLNNLEEHNVPNICKDLALQYIKGG-QYARDLDSTKSVIEDYFNGVKPSEDGFDVVLI 130
H + N+ ++ + D A +Y++ Q A D +K VIED + +K +++ FD V+I
Sbjct: 98 HKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIEDGYKFIKETKEKFDAVVI 157
Query: 131 DIDSLFQWNP 140
D+ + +P
Sbjct: 158 DLTDPIKDSP 167
>PUR2_LACLA (Q9ZF44) Phosphoribosylamine--glycine ligase (EC
6.3.4.13) (GARS) (Glycinamide ribonucleotide synthetase)
(Phosphoribosylglycinamide synthetase)
Length = 412
Score = 32.7 bits (73), Expect = 0.30
Identities = 19/53 (35%), Positives = 26/53 (48%)
Query: 65 YCKIHSLHAKLNNLEEHNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNG 117
Y SL L L+E VP + K L KG A D+++ KS + D F+G
Sbjct: 121 YATFDSLEPALAYLDEKGVPLVIKADGLAAGKGVTVAFDIETAKSALADIFSG 173
>DEGP_BARHE (P54925) Probable periplasmic serine protease DO-like
precursor (EC 3.4.21.-) (Antigen htrA)
Length = 503
Score = 31.6 bits (70), Expect = 0.67
Identities = 13/37 (35%), Positives = 23/37 (62%)
Query: 97 GGQYARDLDSTKSVIEDYFNGVKPSEDGFDVVLIDID 133
G +Y+ + ++ +EDY V PS+DG +V+ D+D
Sbjct: 402 GSKYSNEHGNSDETLEDYGLIVAPSDDGVGLVVTDVD 438
>DDLA_ECOLI (P23844) D-alanine--D-alanine ligase A (EC 6.3.2.4)
(D-alanylalanine synthetase A) (D-Ala-D-Ala ligase A)
Length = 364
Score = 30.4 bits (67), Expect = 1.5
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 6/53 (11%)
Query: 96 KGGQYARDLDSTKSVIEDYFNGVKPSEDGFDVVLIDIDSLFQWNPPHSSNLLL 148
K ++ L S K++++ + FDVVL+ ID QW+ +SN LL
Sbjct: 13 KSAEHEVSLQSAKNIVDAI------DKSRFDVVLLGIDKQGQWHVSDASNYLL 59
>DDLA_ECOL6 (Q8FKE3) D-alanine--D-alanine ligase A (EC 6.3.2.4)
(D-alanylalanine synthetase A) (D-Ala-D-Ala ligase A)
Length = 364
Score = 30.4 bits (67), Expect = 1.5
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 6/53 (11%)
Query: 96 KGGQYARDLDSTKSVIEDYFNGVKPSEDGFDVVLIDIDSLFQWNPPHSSNLLL 148
K ++ L S K++++ + FDVVL+ ID QW+ +SN LL
Sbjct: 13 KSAEHEVSLQSAKNIVDAI------DKSRFDVVLLGIDKQGQWHVSDASNYLL 59
>DLTA_STRPN (Q97N82) D-alanine--poly(phosphoribitol) ligase subunit
1 (EC 6.1.1.13) (D-alanine-activating enzyme) (DAE)
(D-alanine-D-alanyl carrier protein ligase) (DCL)
Length = 516
Score = 30.0 bits (66), Expect = 1.9
Identities = 23/68 (33%), Positives = 37/68 (53%), Gaps = 7/68 (10%)
Query: 50 NGGIIELRNINNDYSYCK-IHSLHAKLNNLEEHNVPNICKDLALQYIKGG---QYARDLD 105
NG IEL +++ + + + I S A ++H V N+ LA +K G Q+ RD+D
Sbjct: 412 NGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNL---LAYVILKDGVREQFERDID 468
Query: 106 STKSVIED 113
TK++ ED
Sbjct: 469 ITKAIKED 476
>RS4E_METAC (Q8TRT5) 30S ribosomal protein S4e
Length = 235
Score = 29.6 bits (65), Expect = 2.5
Identities = 18/69 (26%), Positives = 31/69 (44%)
Query: 53 IIELRNINNDYSYCKIHSLHAKLNNLEEHNVPNICKDLALQYIKGGQYARDLDSTKSVIE 112
+I L +N Y + L+ L E NV +C+ +KGG++ +L+ ++I
Sbjct: 84 VITLPLVNEAYRMLQDEKGRLTLHKLNETNVNKLCRINNKTTVKGGKFQLNLNDGTNIIG 143
Query: 113 DYFNGVKPS 121
G K S
Sbjct: 144 SNEYGTKDS 152
>PANE_SCHPO (Q9HDU6) Probable 2-dehydropantoate 2-reductase (EC
1.1.1.169) (Ketopantoate reductase) (KPA reductase)
(KPR)
Length = 350
Score = 29.6 bits (65), Expect = 2.5
Identities = 21/69 (30%), Positives = 34/69 (48%), Gaps = 7/69 (10%)
Query: 19 TIFIAGFAALGLLLVTLLVSMAMMLQSCQNSNGGIIELRNINNDYSYCKIHSLHAKLNNL 78
TI+I G ++G LL A L S ++ N +I L + + K + K++ L
Sbjct: 4 TIYILGAGSIGSLL-------AYELASLKSINNRVILLLRDKSRVNSFKDKNSTLKIDRL 56
Query: 79 EEHNVPNIC 87
E NVP++C
Sbjct: 57 FEENVPHLC 65
>MR11_SULAC (Q8NKQ0) DNA double-strand break repair protein mre11
Length = 382
Score = 29.6 bits (65), Expect = 2.5
Identities = 12/33 (36%), Positives = 23/33 (69%), Gaps = 1/33 (3%)
Query: 55 ELRNINNDYSYCKIHSLHAKLNNLEEHNVPNIC 87
+L ++NN + K+H++ +L L++HN+P IC
Sbjct: 49 DLFDVNNPPNRAKLHAIK-ELKRLKDHNIPFIC 80
>YF91_MYCTU (O06599) Hypothetical protein Rv1591/MT1626/Mb1617
Length = 193
Score = 29.3 bits (64), Expect = 3.3
Identities = 16/33 (48%), Positives = 20/33 (60%)
Query: 12 YITSFSATIFIAGFAALGLLLVTLLVSMAMMLQ 44
Y+ S S FIA F LGLL V +V+ A+M Q
Sbjct: 38 YLGSESQNFFIAPFMLLGLLSVLAVVASALMWQ 70
>ORP3_MOUSE (Q9DBS9) Oxysterol binding protein-related protein 3
(OSBP-related protein 3) (ORP-3)
Length = 855
Score = 29.3 bits (64), Expect = 3.3
Identities = 10/32 (31%), Positives = 21/32 (65%)
Query: 52 GIIELRNINNDYSYCKIHSLHAKLNNLEEHNV 83
G I+++N+N+D +CK++ + AK + H +
Sbjct: 672 GEIDIKNLNDDSCHCKVNFIKAKYWSTNAHEI 703
>YOJ8_YEAST (Q12496) Hypothetical 118.4 kDa protein in WRS1-PKH2
intergenic region
Length = 1037
Score = 28.9 bits (63), Expect = 4.3
Identities = 21/81 (25%), Positives = 38/81 (45%), Gaps = 8/81 (9%)
Query: 74 KLNNLEEHNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSEDGFDVV----- 128
K + ++ V + ++ +Y+ + + + +VI DY G +EDG +V
Sbjct: 365 KTHTIDLSRVRQVVENTKWEYLLNYEKNGESRFSSAVITDYIYG---NEDGSSLVSSLKD 421
Query: 129 LIDIDSLFQWNPPHSSNLLLR 149
L D D+L QW+ +LL R
Sbjct: 422 LSDFDALLQWSQKDWQSLLNR 442
>DLTA_STRA5 (P59591) D-alanine--poly(phosphoribitol) ligase subunit
1 (EC 6.1.1.13) (D-alanine-activating enzyme) (DAE)
(D-alanine-D-alanyl carrier protein ligase) (DCL)
Length = 511
Score = 28.9 bits (63), Expect = 4.3
Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 1/65 (1%)
Query: 50 NGGIIELRNINNDYSYCK-IHSLHAKLNNLEEHNVPNICKDLALQYIKGGQYARDLDSTK 108
NG IEL +++ + + + + S A ++H V N+ + L+ + RDLD TK
Sbjct: 408 NGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIVLKEGVRDDFERDLDLTK 467
Query: 109 SVIED 113
++ ED
Sbjct: 468 AIKED 472
>DLTA_STRA3 (Q8VM67) D-alanine--poly(phosphoribitol) ligase subunit
1 (EC 6.1.1.13) (D-alanine-activating enzyme) (DAE)
(D-alanine-D-alanyl carrier protein ligase) (DCL)
Length = 511
Score = 28.9 bits (63), Expect = 4.3
Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 1/65 (1%)
Query: 50 NGGIIELRNINNDYSYCK-IHSLHAKLNNLEEHNVPNICKDLALQYIKGGQYARDLDSTK 108
NG IEL +++ + + + + S A ++H V N+ + L+ + RDLD TK
Sbjct: 408 NGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIVLKEGVRDDFERDLDLTK 467
Query: 109 SVIED 113
++ ED
Sbjct: 468 AIKED 472
>DPOL_BPT5 (P19822) DNA polymerase (EC 2.7.7.7)
Length = 829
Score = 28.5 bits (62), Expect = 5.7
Identities = 28/111 (25%), Positives = 43/111 (38%), Gaps = 18/111 (16%)
Query: 43 LQSCQNSNGGIIELRNINNDYSYCKIH------------SLHAKLNN---LEEHNVPNIC 87
LQ +S I N+ D + K H LH + L+E +
Sbjct: 179 LQKILDSENHTIVFHNLKFDMHFYKYHLGLTFDKAHKERRLHDTMLQHYVLDERRGTHGL 238
Query: 88 KDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSEDGFDVVLIDIDSLFQW 138
K LA++Y G Y +LD K +DY K ++ F LI D ++ +
Sbjct: 239 KSLAMKYTDMGDYDFELDKFK---DDYCKAHKIKKEDFTYDLIPFDIMWPY 286
>YVBV_BACSU (O32256) Hypothetical transport protein yvbV
Length = 305
Score = 28.1 bits (61), Expect = 7.4
Identities = 14/43 (32%), Positives = 25/43 (57%)
Query: 1 MGSSFVLESGFYITSFSATIFIAGFAALGLLLVTLLVSMAMML 43
+GS F+L SGF+ SFSA + A F L + ++++ ++
Sbjct: 189 IGSVFLLISGFWTESFSAIQWTAPFITSLLFISVFVIALGWLV 231
>S1A1_MOUSE (Q9QXZ6) Solute carrier organic anion transporter family
member 1A1 (Solute carrier family 21 member 1)
(Sodium-independent organic anion transporting
polypeptide 1) (OATP1)
Length = 670
Score = 28.1 bits (61), Expect = 7.4
Identities = 21/81 (25%), Positives = 33/81 (39%), Gaps = 6/81 (7%)
Query: 7 LESGFYITSFSATIFIAGFAALGLLLVTLLVSMAMMLQSCQNSNGGIIELRNINNDYSYC 66
L SGF + F T+ A + A GL L + + L +C N + + Y
Sbjct: 371 LISGFIMKKFKITVKKAAYIAFGLSLSEYFIFLCNYLLTCDN-----FPVAGLTTSYKGV 425
Query: 67 KIHSLHAKLNNLEEHNVPNIC 87
+ H L+ + N L + N C
Sbjct: 426 Q-HPLYGEKNVLADCNTRCSC 445
>MYCT_RAT (Q921A2) Proton myo-inositol cotransporter
(H(+)-myo-inositol cotransporter) (Hmit)
Length = 618
Score = 28.1 bits (61), Expect = 7.4
Identities = 13/27 (48%), Positives = 17/27 (62%)
Query: 7 LESGFYITSFSATIFIAGFAALGLLLV 33
L + Y+T + A AGFAA+GLL V
Sbjct: 534 LHTAEYLTYYGAFFLYAGFAAVGLLFV 560
>ORP3_HUMAN (Q9H4L5) Oxysterol binding protein-related protein 3
(OSBP-related protein 3) (ORP-3)
Length = 887
Score = 27.7 bits (60), Expect = 9.7
Identities = 10/32 (31%), Positives = 20/32 (62%)
Query: 52 GIIELRNINNDYSYCKIHSLHAKLNNLEEHNV 83
G I ++N+++D YCK++ + AK + H +
Sbjct: 704 GEIVIKNLHDDSCYCKVNFIKAKYWSTNAHEI 735
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.138 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,549,485
Number of Sequences: 164201
Number of extensions: 693751
Number of successful extensions: 1893
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 1878
Number of HSP's gapped (non-prelim): 24
length of query: 149
length of database: 59,974,054
effective HSP length: 100
effective length of query: 49
effective length of database: 43,553,954
effective search space: 2134143746
effective search space used: 2134143746
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC147429.11